##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138685_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6476383
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.830601587336634	33.0	32.0	34.0	28.0	34.0
2	32.19996130556207	33.0	33.0	34.0	29.0	34.0
3	31.983403081627507	33.0	31.0	33.0	29.0	34.0
4	32.57547044391908	33.0	33.0	34.0	31.0	34.0
5	32.871051789247176	33.0	33.0	34.0	31.0	34.0
6	36.59701873715622	38.0	37.0	38.0	34.0	38.0
7	37.06292154123683	38.0	38.0	38.0	36.0	38.0
8	37.01317278487081	38.0	38.0	38.0	36.0	38.0
9	37.117130503245406	38.0	38.0	38.0	36.0	38.0
10-11	37.22251548433748	38.0	38.0	38.0	36.0	38.0
12-13	37.29860726272674	38.0	38.0	38.0	37.0	38.0
14-15	37.25977131062199	38.0	38.0	38.0	37.0	38.0
16-17	37.331574275332386	38.0	38.0	38.0	37.0	38.0
18-19	37.352866021049095	38.0	38.0	38.0	37.0	38.0
20-21	37.36637567362372	38.0	38.0	38.0	37.0	38.0
22-23	37.376361639310474	38.0	38.0	38.0	37.0	38.0
24-25	37.38859559702052	38.0	38.0	38.0	37.0	38.0
26-27	37.35904164513114	38.0	38.0	38.0	37.0	38.0
28-29	37.339326604883155	38.0	38.0	38.0	37.0	38.0
30-31	37.33560405976817	38.0	38.0	38.0	37.0	38.0
32-33	37.32033786759938	38.0	38.0	38.0	37.0	38.0
34-35	37.29934161159957	38.0	38.0	38.0	37.0	38.0
36-37	37.27848877273473	38.0	38.0	38.0	37.0	38.0
38-39	37.27704949610178	38.0	38.0	38.0	37.0	38.0
40-41	37.26385699093838	38.0	38.0	38.0	37.0	38.0
42-43	37.2164797722035	38.0	38.0	38.0	37.0	38.0
44-45	37.13191415087288	38.0	38.0	38.0	36.0	38.0
46-47	37.14976053857244	38.0	38.0	38.0	36.0	38.0
48-49	37.104924264515255	38.0	38.0	38.0	36.0	38.0
50-51	37.06240715687663	38.0	38.0	38.0	36.0	38.0
52-53	37.03894849114913	38.0	38.0	38.0	36.0	38.0
54-55	37.0075916345944	38.0	38.0	38.0	36.0	38.0
56-57	36.959990148750265	38.0	38.0	38.0	36.0	38.0
58-59	36.944452811779826	38.0	38.0	38.0	36.0	38.0
60-61	36.95109550472509	38.0	38.0	38.0	36.0	38.0
62-63	36.95470323856006	38.0	38.0	38.0	36.0	38.0
64-65	36.916949694370565	38.0	38.0	38.0	36.0	38.0
66-67	36.939339427876945	38.0	38.0	38.0	36.0	38.0
68-69	36.921945842229974	38.0	38.0	38.0	36.0	38.0
70-71	36.92184630435992	38.0	38.0	38.0	35.5	38.0
72-73	36.90288069523337	38.0	38.0	38.0	35.0	38.0
74-75	36.89448539937393	38.0	38.0	38.0	35.0	38.0
76-77	36.87769316290114	38.0	38.0	38.0	35.0	38.0
78-79	36.85718731650396	38.0	38.0	38.0	35.0	38.0
80-81	36.81745544225585	38.0	38.0	38.0	35.0	38.0
82-83	36.778915599129974	38.0	38.0	38.0	35.0	38.0
84-85	36.754941297701635	38.0	38.0	38.0	35.0	38.0
86-87	36.743150480360164	38.0	38.0	38.0	35.0	38.0
88-89	36.72291998625535	38.0	38.0	38.0	34.5	38.0
90-91	36.69608065037116	38.0	38.0	38.0	34.0	38.0
92-93	36.66879849728201	38.0	38.0	38.0	34.0	38.0
94-95	36.6372292942103	38.0	38.0	38.0	34.0	38.0
96-97	36.63299891119858	38.0	38.0	38.0	34.0	38.0
98-99	36.637384879017866	38.0	38.0	38.0	34.0	38.0
100	35.16371875942521	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	19.0
21	88.0
22	337.0
23	1166.0
24	2923.0
25	6248.0
26	11315.0
27	18794.0
28	29059.0
29	42277.0
30	57553.0
31	75822.0
32	98058.0
33	131345.0
34	191976.0
35	329472.0
36	850463.0
37	4629463.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.164909024064826	19.318066890114434	13.442148186727065	25.074875899093673
2	14.15488584204137	20.80406077512715	42.95619562206367	22.08485776076781
3	17.958431976144137	26.544803549226494	30.544631844339023	24.952132630290347
4	12.149960865501622	17.822278268595294	40.2094193626288	29.818341503274283
5	13.059048546078882	37.57217570362963	35.954976720802335	13.413799029489146
6	28.972666977747625	38.360667179095856	19.455241863385226	13.211423979771297
7	25.850293288707597	33.061339948548444	24.03103707733159	17.057329685412366
8	23.878868806863338	37.02620737532045	21.720148422352416	17.374775395463796
9	25.88177691158784	16.794513233698503	21.580981235976935	35.74272861873673
10-11	23.80501953096652	26.707033340528707	29.52053322364246	19.967413904862312
12-13	24.862064210841144	24.5820313591707	29.52999382525709	21.025910604731067
14-15	22.08664929174201	25.707211880458587	27.229628019219987	24.97651080857942
16-17	21.36909599077139	28.81607681324591	27.882940215240517	21.93188698074218
18-19	21.40897936394435	27.970851940041225	29.753058149896322	20.867110546118102
20-21	22.307516875355525	27.158143188989776	29.50034958189141	21.033990353763286
22-23	22.09053394745083	27.041655523073572	29.325302019682315	21.542508509793286
24-25	21.76958755205112	27.228096834195416	29.408479979862882	21.593835633890578
26-27	21.690291701481286	27.312004905193543	29.386426081544208	21.61127731178096
28-29	21.658960971363335	27.369399916741255	29.42647332943124	21.54516578246417
30-31	21.829336460528452	27.23363780235775	29.364591056889005	21.572434680224788
32-33	21.638135501032497	27.33524150612075	29.325232974043953	21.7013900188028
34-35	21.80433500342696	27.381347728811722	29.31796962168134	21.49634764607998
36-37	21.827973952684022	27.301495974625524	29.201431240792626	21.66909883189783
38-39	21.673278861325723	27.452191365836715	29.31486389619358	21.559665876643987
40-41	21.84139599263442	27.294113300620943	29.23543213562439	21.629058571120247
42-43	21.775665299242736	27.417407327041506	29.034829543698436	21.772097830017323
44-45	21.84712215037102	27.515633254709993	28.899994411118445	21.737250183800537
46-47	21.845474794195706	27.542748195349127	28.699431273784796	21.912345736670375
48-49	21.826451461236022	27.68771201301272	28.563484098957794	21.922352426793463
50-51	21.84864832675203	27.73975149189746	28.501673675683964	21.909926505666547
52-53	21.968567817228877	27.699230715338974	28.356204873393665	21.975996594038488
54-55	21.988155204529317	27.639279644415442	28.367870624559565	22.004694526495676
56-57	21.937859091643997	27.721902326819816	28.359256654415216	21.980981927120975
58-59	21.907428026302224	27.70605873809356	28.423060902193104	21.963452333411116
60-61	21.97405832521199	27.676237135862607	28.473403005056998	21.876301533868403
62-63	21.942103032229085	27.689308164436337	28.40151770483759	21.967071098496984
64-65	21.912080433404245	27.635728222160594	28.435596306789268	22.016595037645892
66-67	21.95559630786004	27.607354056986456	28.342546147336055	22.094503487817445
68-69	21.908063051763346	27.58328713243533	28.47057623317606	22.038073582625266
70-71	21.94335045712253	27.414473652786942	28.482914827420664	22.15926106266986
72-73	22.03316998902286	27.510812094247676	28.400274451222764	22.0557434655067
74-75	21.901369583876093	27.619567554226755	28.359918204922085	22.119144656975067
76-77	22.006329633073946	27.6049866041748	28.268855851334163	22.119827911417094
78-79	21.983735415553717	27.561909625192254	28.252980273864704	22.20137468538932
80-81	22.032225485505617	27.668023872151227	28.18192689365024	22.11782374869292
82-83	22.026418975597057	27.66581386402816	28.248694201015734	22.059072959359053
84-85	22.12353389371454	27.657267569628942	28.13902462283615	22.080173913820364
86-87	22.013336580860383	27.697889310225648	28.2016027370294	22.08717137188457
88-89	22.131611990365833	27.603231994318033	28.12872038985294	22.136435625463196
90-91	22.130857378090933	27.70420774300898	28.100718464730416	22.064216414169675
92-93	22.127129456581503	27.64738296829283	28.15917758174859	22.066309993377075
94-95	21.980471656099326	27.76041867780807	28.127673048259673	22.131436617832936
96-97	22.116228752341648	27.846820164482594	28.139077257718515	21.897873825457243
98-99	23.203918171834072	29.781577920373774	28.364581166177828	18.64992274161433
100	29.391061195487143	40.90620670793737	29.702732096575485	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1327.0
1	1264.0
2	1788.5
3	2542.0
4	3179.0
5	4547.0
6	5781.5
7	7172.0
8	8881.5
9	9486.0
10	8954.0
11	8498.0
12	9108.5
13	11281.5
14	15524.0
15	20398.5
16	24298.0
17	26604.0
18	26493.0
19	24700.5
20	22954.5
21	22132.5
22	23571.0
23	27948.5
24	35082.5
25	45143.5
26	57558.5
27	71632.0
28	86322.5
29	102095.0
30	117606.0
31	133028.0
32	150186.5
33	166956.5
34	181028.0
35	194939.0
36	210829.0
37	224917.0
38	235454.0
39	241394.0
40	247474.5
41	254538.0
42	259377.0
43	263755.0
44	266696.5
45	268254.5
46	268598.0
47	268403.5
48	266791.0
49	262237.5
50	256143.5
51	247694.0
52	236143.0
53	222762.5
54	208533.0
55	192665.0
56	174690.0
57	156919.0
58	139729.0
59	120327.0
60	100172.5
61	81269.0
62	63759.5
63	49094.5
64	37284.5
65	27727.0
66	19941.0
67	14563.0
68	10811.0
69	7777.0
70	5601.5
71	3978.5
72	2763.0
73	1898.0
74	1256.0
75	780.0
76	491.0
77	326.0
78	207.5
79	129.5
80	85.5
81	56.5
82	39.5
83	29.5
84	20.0
85	14.0
86	7.5
87	3.0
88	2.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002794769858422518
3	0.0023933112047264653
4	0.0
5	0.0
6	6.330694154437748E-4
7	0.0
8	0.0
9	0.0
10-11	1.5440717449848162E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.1618009160476965E-4
24-25	0.001189063947241387
26-27	0.003081095594349904
28-29	0.0019929789976319856
30-31	0.0025207039877769054
32-33	0.0033005310136023716
34-35	9.632523126794689E-4
36-37	0.0
38-39	4.7060931120915295E-5
40-41	1.5788340247573804E-5
42-43	5.72063062007266E-4
44-45	0.0
46-47	0.0
48-49	2.2831569635349663E-4
50-51	0.0061356666632122695
52-53	0.0
54-55	0.002925009280636589
56-57	0.04693824476423185
58-59	0.05833134740733071
60-61	0.037811793793043336
62-63	0.008657042300267129
64-65	0.0
66-67	0.0
68-69	6.331496842544948E-4
70-71	0.002658482984897834
72-73	0.009997256672645293
74-75	0.004808972089112184
76-77	0.004050028252662685
78-79	0.003746594261306827
80-81	0.016030482227864757
82-83	0.05348815989780679
84-85	0.03477299891196044
86-87	0.009645480984223702
88-89	0.008237216757595285
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	39.0
20-21	181.0
22-23	370.0
24-25	561.0
26-27	1623.0
28-29	4284.0
30-31	12289.0
32-33	15709.0
34-35	21721.0
36-37	34580.0
38-39	41258.0
40-41	40136.0
42-43	45799.0
44-45	55326.0
46-47	56913.0
48-49	53604.0
50-51	49939.0
52-53	46890.0
54-55	50490.0
56-57	54241.0
58-59	54566.0
60-61	53378.0
62-63	50610.0
64-65	53250.0
66-67	57065.0
68-69	58812.0
70-71	57511.0
72-73	54142.0
74-75	54334.0
76-77	57117.0
78-79	59704.0
80-81	59841.0
82-83	56042.0
84-85	54478.0
86-87	56625.0
88-89	60223.0
90-91	60664.0
92-93	60325.0
94-95	68173.0
96-97	199506.0
98-99	1474160.0
100-101	3129904.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.18824089847517
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.67261747494543	79.05586015300833
2	6.783978359882181	11.829662789829003
3	1.5227057354087863	3.982861034289332
4	0.5129858943366105	1.789053509317605
5	0.20668652630433643	0.9010317322945756
6	0.1102964560987299	0.5769932390750493
7	0.0593820466504352	0.36241913318818453
8	0.03543855489351165	0.24718602089194644
9	0.025066548220016277	0.1966957420230021
>10	0.06939851275480544	0.8875569567490131
>50	9.138536811165319E-4	0.05185559345496967
>100	4.5252330340317447E-4	0.07505306644211054
>500	7.751352068178561E-5	0.0437710294368225
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5440717449848162E-5	0.0	0.0	0.0
3	0.0	1.5440717449848162E-5	0.0	0.0	0.0
4	0.0	3.0881434899696325E-5	0.0	0.0	0.0
5	0.0	3.0881434899696325E-5	0.0	0.0	0.0
6	0.0	3.0881434899696325E-5	0.0	0.0	1.5440717449848162E-5
7	0.0	3.0881434899696325E-5	0.0	0.0	1.5440717449848162E-5
8	0.0	3.0881434899696325E-5	0.0	0.0	3.0881434899696325E-5
9	0.0	6.176286979939265E-5	0.0	1.5440717449848162E-5	3.0881434899696325E-5
10-11	1.5440717449848162E-5	6.176286979939265E-5	0.0	1.5440717449848162E-5	3.0881434899696325E-5
12-13	1.5440717449848162E-5	6.176286979939265E-5	0.0	1.5440717449848162E-5	3.0881434899696325E-5
14-15	2.3161076174772244E-5	1.0036466342401308E-4	0.0	1.5440717449848162E-5	3.860179362462041E-5
16-17	6.176286979939265E-5	1.0808502214893715E-4	0.0	1.5440717449848162E-5	4.6322152349544494E-5
18-19	6.176286979939265E-5	1.1580538087386122E-4	0.0	3.860179362462041E-5	4.6322152349544494E-5
20-21	6.176286979939265E-5	1.235257395987853E-4	0.0	4.6322152349544494E-5	4.6322152349544494E-5
22-23	8.49239459741649E-5	1.235257395987853E-4	0.0	4.6322152349544494E-5	4.6322152349544494E-5
24-25	1.0808502214893715E-4	1.3124609832370937E-4	0.0	4.6322152349544494E-5	4.6322152349544494E-5
26-27	1.1580538087386122E-4	1.3896645704863347E-4	0.0	8.49239459741649E-5	4.6322152349544494E-5
28-29	1.235257395987853E-4	1.3896645704863347E-4	0.0	4.400604473206726E-4	4.6322152349544494E-5
30-31	1.235257395987853E-4	2.0072932684802612E-4	0.0	0.0013510627768617144	4.6322152349544494E-5
32-33	1.235257395987853E-4	2.0072932684802612E-4	0.0	0.0031807877946687215	4.6322152349544494E-5
34-35	1.3124609832370937E-4	2.5477183792249467E-4	0.0	0.005234403215498527	4.6322152349544494E-5
36-37	1.3896645704863347E-4	3.0881434899696325E-4	0.0	0.008222182042044147	4.6322152349544494E-5
38-39	1.3896645704863347E-4	3.2425506644681145E-4	0.0	0.013425703822642979	4.6322152349544494E-5
40-41	1.5440717449848162E-4	3.5513650134650775E-4	0.0	0.01847481842874333	4.6322152349544494E-5
42-43	1.5440717449848162E-4	3.8601793624620405E-4	0.0	0.023778704872766173	4.6322152349544494E-5
44-45	1.6212753322340573E-4	4.4778080604559675E-4	0.0	0.029800584678206954	4.6322152349544494E-5
46-47	1.8528860939817795E-4	4.786622409452931E-4	0.0	0.03609267703902008	4.6322152349544494E-5
48-49	2.0072932684802612E-4	4.863825996702172E-4	0.0	0.04287887235822835	6.176286979939265E-5
50-51	2.3161076174772245E-4	5.481454694696098E-4	0.0	0.049626465883812	6.176286979939265E-5
52-53	2.3161076174772245E-4	5.71306545644382E-4	0.0	0.05679867913926647	7.720358724924083E-5
54-55	2.3161076174772245E-4	5.71306545644382E-4	0.0	0.0634459080014261	9.264430469908899E-5
56-57	2.3161076174772245E-4	5.71306545644382E-4	0.0	0.07006997578741096	9.264430469908899E-5
58-59	2.3161076174772245E-4	5.71306545644382E-4	0.0	0.07687933218279401	1.1580538087386122E-4
60-61	2.3161076174772245E-4	5.71306545644382E-4	0.0	0.08406698615569833	1.235257395987853E-4
62-63	2.3161076174772245E-4	6.176286979939265E-4	0.0	0.09092266470343091	1.235257395987853E-4
64-65	2.3161076174772245E-4	7.874765899422563E-4	0.0	0.09785554683841274	1.235257395987853E-4
66-67	2.3161076174772245E-4	7.951969486671804E-4	0.0	0.10453365713547208	1.235257395987853E-4
68-69	2.3161076174772245E-4	8.337987422918009E-4	0.0	0.11101103810568337	1.3124609832370937E-4
70-71	2.3161076174772245E-4	8.49239459741649E-4	0.0	0.11678586643192658	1.3896645704863347E-4
72-73	2.470514791975706E-4	8.569598184665732E-4	0.0	0.12152616668902996	1.698478919483298E-4
74-75	2.470514791975706E-4	8.646801771914972E-4	0.0	0.12499260775652088	1.698478919483298E-4
76-77	2.547718379224947E-4	8.724005359164212E-4	0.0	0.1284976506176364	1.698478919483298E-4
78-79	3.0881434899696325E-4	8.801208946413454E-4	0.0	0.1307056732129647	1.698478919483298E-4
80-81	3.0881434899696325E-4	8.801208946413454E-4	0.0	0.13167071805358022	1.7756825067325387E-4
82-83	3.165347077218873E-4	8.801208946413454E-4	0.0	0.1322420245992246	2.470514791975706E-4
84-85	3.2425506644681145E-4	8.801208946413454E-4	0.0	0.13248907607842217	2.470514791975706E-4
86-87	3.2425506644681145E-4	8.801208946413454E-4	0.0	0.1325894407418462	2.547718379224947E-4
88	3.2425506644681145E-4	9.110023295410416E-4	0.0	0.13260488145929603	2.624921966474188E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8365	0.0	47.980095	1
GTATCAA	16040	0.0	38.48527	1
TCAACGC	18105	0.0	32.92358	4
ATCAACG	18170	0.0	32.8058	3
CAACGCA	18470	0.0	32.22701	5
TATCAAC	19265	0.0	31.271973	2
AACGCAG	19080	0.0	31.19669	6
ACGCAGA	21750	0.0	27.386324	7
CGCAGAG	22140	0.0	26.75061	8
GTACATG	21165	0.0	23.353048	1
TACATGG	21145	0.0	22.85346	2
GCAGAGT	26210	0.0	22.59666	9
ACATGGG	21070	0.0	22.249844	3
GAGTACT	15230	0.0	19.847578	12-13
GTGGTAT	4380	0.0	19.372774	1
CAGAGTA	25835	0.0	19.221495	10-11
CATGGGG	14755	0.0	19.207308	4
TGGTATC	4135	0.0	19.084173	2
AGAGTAC	24235	0.0	18.486092	10-11
GTACTTT	17325	0.0	17.46012	14-15
>>END_MODULE