FastQCFastQC Report
Fri 27 May 2016
SRR2138684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7018630
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73650.045.7593961
GTATCAA135500.037.6554641
TCAACGC153100.032.571044
ATCAACG156150.031.7983553
CAACGCA157200.031.7215395
AACGCAG160600.031.0766056
TATCAAC168900.029.8039382
ACGCAGA188750.026.4190257
CGCAGAG195600.025.4282268
GTACATG177550.021.4867631
GCAGAGT235250.021.2151649
TACATGG178950.020.8406682
ACATGGG176700.020.5729623
GTGGTAT42350.018.5819471
AGAGTAC206950.018.43336510-11
CATGGGG125800.018.4258234
GAGTACT144100.018.40063712-13
CAGAGTA229100.018.06055510-11
TGGTATC41800.016.473142
GTACTTT164650.015.85740914-15
Produced by FastQC (version 0.11.5)