##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138684_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7018630
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.776981120247115	33.0	32.0	34.0	28.0	34.0
2	32.16029752245096	33.0	32.0	34.0	29.0	34.0
3	31.97966654461056	33.0	31.0	33.0	29.0	34.0
4	32.5674774136833	33.0	33.0	34.0	31.0	34.0
5	32.85971493012169	33.0	33.0	34.0	31.0	34.0
6	36.58494991187739	38.0	37.0	38.0	34.0	38.0
7	37.04370283089435	38.0	38.0	38.0	36.0	38.0
8	36.99069519265156	38.0	38.0	38.0	35.0	38.0
9	37.10060025959482	38.0	38.0	38.0	36.0	38.0
10-11	37.205150435341366	38.0	38.0	38.0	36.0	38.0
12-13	37.285517900216995	38.0	38.0	38.0	36.5	38.0
14-15	37.244818433226996	38.0	38.0	38.0	36.5	38.0
16-17	37.31481457207461	38.0	38.0	38.0	37.0	38.0
18-19	37.33469558874025	38.0	38.0	38.0	37.0	38.0
20-21	37.34930537405032	38.0	38.0	38.0	37.0	38.0
22-23	37.35938725251431	38.0	38.0	38.0	37.0	38.0
24-25	37.37339782557277	38.0	38.0	38.0	37.0	38.0
26-27	37.34090499756186	38.0	38.0	38.0	37.0	38.0
28-29	37.32167410166149	38.0	38.0	38.0	37.0	38.0
30-31	37.318826894098834	38.0	38.0	38.0	37.0	38.0
32-33	37.302828069364104	38.0	38.0	38.0	37.0	38.0
34-35	37.28167747409826	38.0	38.0	38.0	37.0	38.0
36-37	37.26280265044768	38.0	38.0	38.0	37.0	38.0
38-39	37.26161617864898	38.0	38.0	38.0	37.0	38.0
40-41	37.25166828816239	38.0	38.0	38.0	37.0	38.0
42-43	37.208325648221745	38.0	38.0	38.0	37.0	38.0
44-45	37.12916042360533	38.0	38.0	38.0	36.0	38.0
46-47	37.15415910030704	38.0	38.0	38.0	36.0	38.0
48-49	37.11577290568467	38.0	38.0	38.0	36.0	38.0
50-51	37.07322812872651	38.0	38.0	38.0	36.0	38.0
52-53	37.04904807678078	38.0	38.0	38.0	36.0	38.0
54-55	37.01601196671897	38.0	38.0	38.0	36.0	38.0
56-57	36.96781191311012	38.0	38.0	38.0	36.0	38.0
58-59	36.951822102415406	38.0	38.0	38.0	36.0	38.0
60-61	36.9564095947934	38.0	38.0	38.0	36.0	38.0
62-63	36.958352835470635	38.0	38.0	38.0	36.0	38.0
64-65	36.917049644162304	38.0	38.0	38.0	36.0	38.0
66-67	36.93759249801685	38.0	38.0	38.0	36.0	38.0
68-69	36.919095037270054	38.0	38.0	38.0	35.5	38.0
70-71	36.91907565721296	38.0	38.0	38.0	35.5	38.0
72-73	36.89786425824599	38.0	38.0	38.0	35.0	38.0
74-75	36.88433877714414	38.0	38.0	38.0	35.0	38.0
76-77	36.86681613559817	38.0	38.0	38.0	35.0	38.0
78-79	36.84094349026458	38.0	38.0	38.0	35.0	38.0
80-81	36.802699525605505	38.0	38.0	38.0	35.0	38.0
82-83	36.7631188906678	38.0	38.0	38.0	35.0	38.0
84-85	36.7347050830827	38.0	38.0	38.0	35.0	38.0
86-87	36.72274376255591	38.0	38.0	38.0	35.0	38.0
88-89	36.702453134211765	38.0	38.0	38.0	34.5	38.0
90-91	36.675047893514176	38.0	38.0	38.0	34.0	38.0
92-93	36.64359095538852	38.0	38.0	38.0	34.0	38.0
94-95	36.611757928242895	38.0	38.0	38.0	34.0	38.0
96-97	36.61151663785314	38.0	38.0	38.0	34.0	38.0
98-99	36.61603057866452	38.0	38.0	38.0	34.0	38.0
100	35.146950011163646	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	7.0
20	9.0
21	108.0
22	390.0
23	1384.0
24	3521.0
25	7042.0
26	12555.0
27	20806.0
28	32101.0
29	46143.0
30	62350.0
31	81729.0
32	106944.0
33	144350.0
34	211012.0
35	362435.0
36	929912.0
37	4995829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93791380939015	19.22692890207918	13.306742198976155	25.528415089554514
2	14.279581480956939	20.586989001294015	42.91111072482832	22.222318792920728
3	18.192316482324024	26.065576853699362	30.25330941922806	25.488797244748557
4	12.236918031011749	17.56272947854496	40.03118842281186	30.16916406763143
5	13.283959975094856	37.25437870353616	36.04625119147184	13.415410129897145
6	29.543372411480036	38.216572397916046	19.184334280437685	13.055720910166235
7	26.012768873697574	32.59824210707788	24.14586607357846	17.24312294564609
8	23.71763435314299	37.372478674613134	21.706814577773727	17.203072394470144
9	25.65848320826144	16.78747846802011	21.62194046416466	35.932097859553785
10-11	23.97155655125499	26.613076899568057	29.572836060492445	19.842530488684506
12-13	24.76249638462207	24.362054987939242	29.548978931785836	21.326469695652854
14-15	22.004778710375103	25.580070469593068	26.97740157267159	25.43774924736024
16-17	21.64649653849825	28.61210806097486	27.71188251838322	22.029512882143667
18-19	21.683042132153997	27.962622335128078	29.251613206565953	21.10272232615197
20-21	22.398391805262424	27.199224683492453	29.078151864066566	21.324231647178557
22-23	22.16343712773731	27.08734797896959	28.93150897291778	21.817705920375317
24-25	21.932830232287568	27.202481321089454	29.025098696193556	21.839589750429422
26-27	21.87293361105601	27.330972234573053	28.965557144062366	21.830537010308575
28-29	21.87519422543893	27.363363651033406	28.98874903706948	21.77269308645819
30-31	22.062830318278998	27.273842034450652	28.882325175320272	21.781002471950075
32-33	21.879294099908066	27.39011981407434	28.87743934530843	21.853146740709164
34-35	22.041022012917583	27.374926506983744	28.86827165124492	21.715779828853755
36-37	22.039590654622025	27.287490388001352	28.82535267950183	21.847566277874797
38-39	21.859593510663533	27.46844771209254	28.93233055985826	21.739628217385665
40-41	22.11821163852884	27.289056377772315	28.823517403612993	21.76921458008585
42-43	21.959959573487435	27.40670293323108	28.641462251977707	21.991875241303784
44-45	22.00282947613495	27.53459435874759	28.512824194867935	21.949751970249526
46-47	22.102478089483665	27.483725820116618	28.35540750119253	22.058388589207187
48-49	22.045134133908473	27.556735444946533	28.344965861343557	22.053164559801438
50-51	22.039621917522485	27.68373089686076	28.233265000453645	22.043382185163114
52-53	22.195754216470963	27.604765485794715	28.133370817797875	22.06610947993645
54-55	22.166433914506495	27.514684794447287	28.14295888716305	22.17592240388317
56-57	22.1048341605737	27.636513938547086	28.169225116181874	22.089426784697345
58-59	22.116373126966877	27.625711583992757	28.146139111146063	22.111776177894306
60-61	22.167186256626586	27.613385850550266	28.182912399446696	22.036515493376456
62-63	22.13636974335117	27.63067006924001	28.22037933299662	22.0125808544122
64-65	22.12163835366667	27.52582686347815	28.201435143351283	22.151099639503894
66-67	22.128126367685297	27.614402852508817	28.026285302371086	22.2311854774348
68-69	22.095511556023588	27.561126817493424	28.195805643185288	22.147555983297696
70-71	22.079924837314742	27.374342513147276	28.302620053265944	22.24311259627204
72-73	22.18321944406129	27.45736763556427	28.1861098581013	22.17330306227314
74-75	22.031912261557096	27.579638301168863	28.13399868843182	22.25445074884222
76-77	22.17096637998159	27.537056549984186	28.037692391272323	22.254284678761906
78-79	22.13433785714231	27.52360206437934	27.990589285395934	22.35147079308242
80-81	22.140456957085263	27.60278309451765	27.968609864310817	22.288150084086265
82-83	22.21203048593309	27.56425262010011	28.041293881462177	22.182423012504625
84-85	22.29291438365844	27.549864139969777	27.948828329961966	22.20839314640982
86-87	22.171525527317073	27.605582144565354	27.999988260399284	22.222904067718286
88-89	22.26027680476462	27.58228023785707	27.913157646828708	22.244285310549607
90-91	22.26953196808038	27.64018110842268	27.913078357703498	22.177208565793446
92-93	22.32376823263676	27.59528469628959	27.915476559382018	22.165470511691634
94-95	22.168914266714957	27.626868885048335	27.962996627552755	22.241220220683953
96-97	22.30769572928898	27.755566317164643	27.95412084399491	21.982617109551466
98-99	23.363535451843116	29.668815536238956	28.192461667526338	18.775187344391586
100	29.71238863830137	40.7381367471336	29.549474614565028	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1169.0
1	1128.5
2	1586.5
3	2258.5
4	2941.5
5	4268.0
6	5441.0
7	6501.0
8	7604.0
9	8032.5
10	7593.0
11	7209.0
12	7909.0
13	9954.5
14	13865.0
15	18292.0
16	21605.0
17	23624.0
18	23916.5
19	22789.0
20	21561.0
21	21268.0
22	23032.5
23	27590.0
24	35019.0
25	45477.0
26	59379.0
27	74928.0
28	90169.5
29	106807.5
30	123412.5
31	138393.5
32	155350.0
33	174037.0
34	190530.0
35	206440.5
36	223513.5
37	237167.5
38	248313.0
39	257163.0
40	265781.0
41	273541.5
42	276394.5
43	279468.5
44	285469.5
45	290530.5
46	291948.5
47	291401.0
48	289310.0
49	285608.5
50	280404.5
51	272377.0
52	259999.0
53	245617.0
54	229491.0
55	212570.0
56	194565.5
57	174771.0
58	154502.0
59	132655.5
60	110626.0
61	89406.0
62	70621.5
63	54387.0
64	40161.5
65	29735.0
66	22098.0
67	16521.0
68	12457.5
69	9149.0
70	6636.0
71	4710.5
72	3334.5
73	2261.5
74	1511.0
75	964.5
76	607.0
77	375.5
78	227.5
79	152.5
80	94.0
81	61.0
82	42.0
83	27.0
84	23.0
85	17.0
86	9.0
87	5.0
88	4.0
89	3.5
90	3.0
91	3.5
92	3.5
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002393629525990115
3	0.0025361074739657166
4	0.0
5	0.0
6	6.269029710926491E-4
7	0.0
8	0.0
9	0.0
10-11	2.137169219634031E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.5647121399153064E-4
24-25	0.001275298647869952
26-27	0.0028073599572711262
28-29	0.0017462165723365227
30-31	0.0024823746053077877
32-33	0.003794403091087969
34-35	0.0010456687219786746
36-37	7.1865405294654985E-6
38-39	2.8893366718655465E-5
40-41	0.0
42-43	5.918392383423562E-4
44-45	0.0
46-47	0.0
48-49	1.869276276042757E-4
50-51	0.006382251343305671
52-53	0.0
54-55	0.00285181757114138
56-57	0.04634715366968022
58-59	0.0579222133670569
60-61	0.03693874629063948
62-63	0.00856342700283376
64-65	0.0
66-67	0.0
68-69	6.254714897504312E-4
70-71	0.0024693732090891314
72-73	0.009965094832414938
74-75	0.004907897082317892
76-77	0.0036301112494024033
78-79	0.003386323178060038
80-81	0.015570542705834427
82-83	0.052134617630092556
84-85	0.03394137630696525
86-87	0.009310770854459314
88-89	0.007882762734863859
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	43.0
20-21	176.0
22-23	340.0
24-25	543.0
26-27	1583.0
28-29	3808.0
30-31	11644.0
32-33	14771.0
34-35	20623.0
36-37	33189.0
38-39	39655.0
40-41	38863.0
42-43	43787.0
44-45	53599.0
46-47	55627.0
48-49	52385.0
50-51	48774.0
52-53	46978.0
54-55	51691.0
56-57	54720.0
58-59	55170.0
60-61	54431.0
62-63	51953.0
64-65	54723.0
66-67	59065.0
68-69	60721.0
70-71	59739.0
72-73	56029.0
74-75	56681.0
76-77	59788.0
78-79	62533.0
80-81	62293.0
82-83	58004.0
84-85	57559.0
86-87	59103.0
88-89	62128.0
90-91	64307.0
92-93	63125.0
94-95	73429.0
96-97	218818.0
98-99	1638580.0
100-101	3457652.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.31539811842312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.50575547761734	78.12040316059195
2	6.692905330530298	11.554015763472778
3	1.614627121389127	4.181015483865179
4	0.5508491455933224	1.9018705322032339
5	0.254737436677505	1.099388163124272
6	0.12715141456872736	0.658507498189222
7	0.07754875750528414	0.46855563143603574
8	0.04733476301076253	0.3268575131292141
9	0.028448520678812532	0.22099908494447065
>10	0.09832808794181895	1.2682247703991494
>50	0.0018899524193603857	0.10842733338432639
>100	3.477315610288404E-4	0.04890522446129417
>500	7.626050663243088E-5	0.0428298407988741
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4247794797560207E-5	0.0	1.4247794797560207E-5	0.0
3	0.0	1.4247794797560207E-5	0.0	2.8495589595120414E-5	0.0
4	0.0	2.8495589595120414E-5	0.0	2.8495589595120414E-5	0.0
5	0.0	5.699117919024083E-5	0.0	4.274338439268062E-5	0.0
6	0.0	5.699117919024083E-5	0.0	4.274338439268062E-5	1.4247794797560207E-5
7	0.0	5.699117919024083E-5	0.0	4.274338439268062E-5	1.4247794797560207E-5
8	0.0	7.123897398780104E-5	0.0	4.274338439268062E-5	4.274338439268062E-5
9	0.0	1.1398235838048166E-4	0.0	4.274338439268062E-5	4.274338439268062E-5
10-11	0.0	1.1398235838048166E-4	0.0	4.274338439268062E-5	4.274338439268062E-5
12-13	0.0	1.2110625577926176E-4	0.0	4.274338439268062E-5	4.274338439268062E-5
14-15	0.0	2.137169219634031E-4	0.0	5.699117919024083E-5	7.123897398780104E-5
16-17	7.1238973987801036E-6	2.137169219634031E-4	0.0	5.699117919024083E-5	8.548676878536125E-5
18-19	1.4247794797560207E-5	2.5646030635608375E-4	0.0	5.699117919024083E-5	1.0685846098170155E-4
20-21	2.8495589595120414E-5	3.134514855463246E-4	0.0	5.699117919024083E-5	1.1398235838048166E-4
22-23	2.8495589595120414E-5	3.2769928034388475E-4	0.0	8.548676878536125E-5	1.2110625577926176E-4
24-25	5.6991179190240835E-5	3.41947075141445E-4	0.0	9.973456358292145E-5	1.2823015317804188E-4
26-27	7.123897398780104E-5	3.561948699390052E-4	0.0	1.1398235838048167E-4	1.2823015317804188E-4
28-29	7.123897398780104E-5	3.561948699390052E-4	0.0	2.4933640895730364E-4	1.2823015317804188E-4
30-31	7.836287138658114E-5	4.274338439268062E-4	0.0	0.0010044695332279948	1.35354050576822E-4
32-33	8.548676878536125E-5	4.5592943352192663E-4	0.0	0.0026287181401498582	1.4247794797560208E-4
34-35	8.548676878536125E-5	4.773011257182669E-4	0.0	0.004352701310654643	1.4247794797560208E-4
36-37	8.548676878536125E-5	4.986728179146073E-4	0.0	0.007358986012939848	1.4247794797560208E-4
38-39	8.548676878536125E-5	5.129206127121675E-4	0.0	0.012245979628502998	1.4247794797560208E-4
40-41	8.548676878536125E-5	5.556639971048481E-4	0.0	0.017296822884238092	1.4247794797560208E-4
42-43	8.548676878536125E-5	5.699117919024083E-4	0.0	0.022269303268586605	1.4247794797560208E-4
44-45	9.261066618414135E-5	6.19779073693869E-4	0.0	0.02797554508500947	1.4247794797560208E-4
46-47	9.973456358292145E-5	6.553985606877695E-4	0.0	0.03385988433640183	1.4247794797560208E-4
48-49	9.973456358292145E-5	6.625224580865496E-4	0.0	0.03968010851120518	1.567257427731623E-4
50-51	9.973456358292145E-5	7.408853294731308E-4	0.0	0.04649767832183774	1.709735375707225E-4
52-53	1.0685846098170155E-4	7.408853294731308E-4	0.0	0.052431884855021565	1.7809743496950258E-4
54-55	1.1398235838048166E-4	7.408853294731308E-4	0.0	0.05884339251392366	1.852213323682827E-4
56-57	1.1398235838048166E-4	7.55133124270691E-4	0.0	0.0663092369878452	1.994691271658429E-4
58-59	1.1398235838048166E-4	7.765048164670313E-4	0.0	0.07368959469298139	2.137169219634031E-4
60-61	1.1398235838048166E-4	7.907526112645915E-4	0.0	0.08058552737500052	2.2084081936218322E-4
62-63	1.1398235838048166E-4	8.121243034609318E-4	0.0	0.08733898210904407	2.4221251155852352E-4
64-65	1.1398235838048166E-4	8.406198930560522E-4	0.0	0.09396420668990957	2.4221251155852352E-4
66-67	1.1398235838048166E-4	8.406198930560522E-4	0.0	0.10071053752655433	2.4221251155852352E-4
68-69	1.1398235838048166E-4	8.548676878536124E-4	0.0	0.10733576210741982	2.5646030635608375E-4
70-71	1.1398235838048166E-4	8.90487174847513E-4	0.0	0.11265731346430857	2.7070810115364393E-4
72-73	1.1398235838048166E-4	8.976110722462931E-4	0.0	0.11696014749317174	2.9920369074876434E-4
74-75	1.1398235838048166E-4	9.118588670438534E-4	0.0	0.1205933351665496	2.9920369074876434E-4
76-77	1.1398235838048166E-4	9.617261488353141E-4	0.0	0.1237777173038043	2.9920369074876434E-4
78-79	1.1398235838048166E-4	0.0010044695332279948	0.0	0.12587926703644442	2.9920369074876434E-4
80-81	1.1398235838048166E-4	0.0010115934306267748	0.0	0.1266771435451078	3.205753829451047E-4
82-83	1.1398235838048166E-4	0.0010115934306267748	0.0	0.12717581636302241	3.561948699390052E-4
84-85	1.1398235838048166E-4	0.0010115934306267748	0.0	0.1274322766693785	3.561948699390052E-4
86-87	1.1398235838048166E-4	0.001025841225424335	0.0	0.12759612630955042	3.561948699390052E-4
88	1.1398235838048166E-4	0.0011113279942096962	0.0	0.1276032502069492	3.561948699390052E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7365	0.0	45.759396	1
GTATCAA	13550	0.0	37.655464	1
TCAACGC	15310	0.0	32.57104	4
ATCAACG	15615	0.0	31.798355	3
CAACGCA	15720	0.0	31.721539	5
AACGCAG	16060	0.0	31.076605	6
TATCAAC	16890	0.0	29.803938	2
ACGCAGA	18875	0.0	26.419025	7
CGCAGAG	19560	0.0	25.428226	8
GTACATG	17755	0.0	21.486763	1
GCAGAGT	23525	0.0	21.215164	9
TACATGG	17895	0.0	20.840668	2
ACATGGG	17670	0.0	20.572962	3
GTGGTAT	4235	0.0	18.581947	1
AGAGTAC	20695	0.0	18.433365	10-11
CATGGGG	12580	0.0	18.425823	4
GAGTACT	14410	0.0	18.400637	12-13
CAGAGTA	22910	0.0	18.060555	10-11
TGGTATC	4180	0.0	16.47314	2
GTACTTT	16465	0.0	15.857409	14-15
>>END_MODULE