FastQCFastQC Report
Fri 27 May 2016
SRR2138683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6797178
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72300.046.8940051
GTATCAA130750.037.3793221
TCAACGC149150.031.7374654
ATCAACG150950.031.3029693
CAACGCA152500.030.9843545
AACGCAG158150.029.9043836
TATCAAC161150.029.6398642
ACGCAGA182100.025.9947457
CGCAGAG186950.025.2747488
GTACATG167750.023.0586171
TACATGG167100.022.6123542
ACATGGG166650.021.9563873
GCAGAGT226000.021.0019329
CATGGGG124400.019.91724
GAGTACT144600.019.05183212-13
CAGAGTA219850.018.3403510-11
AGAGTAC202350.018.31424910-11
GTACTTT166550.016.36189314-15
TGCGCGA4959.128553E-615.8979194
AGTACTT162800.015.73008612-13
Produced by FastQC (version 0.11.5)