##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138683_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6797178
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.850428221829706	33.0	32.0	34.0	28.0	34.0
2	32.20714302317815	33.0	33.0	34.0	29.0	34.0
3	31.999178041240057	33.0	31.0	33.0	29.0	34.0
4	32.57087544272049	33.0	33.0	34.0	31.0	34.0
5	32.873461162853175	33.0	33.0	34.0	31.0	34.0
6	36.5989019266525	38.0	37.0	38.0	34.0	38.0
7	37.07265029693205	38.0	38.0	38.0	36.0	38.0
8	37.01907674037667	38.0	38.0	38.0	36.0	38.0
9	37.129614672442	38.0	38.0	38.0	36.0	38.0
10-11	37.23263227768936	38.0	38.0	38.0	36.0	38.0
12-13	37.30782546815752	38.0	38.0	38.0	37.0	38.0
14-15	37.26807272076736	38.0	38.0	38.0	37.0	38.0
16-17	37.338040360278924	38.0	38.0	38.0	37.0	38.0
18-19	37.35689774491708	38.0	38.0	38.0	37.0	38.0
20-21	37.370226839671204	38.0	38.0	38.0	37.0	38.0
22-23	37.3818909835919	38.0	38.0	38.0	37.0	38.0
24-25	37.39408309420904	38.0	38.0	38.0	37.0	38.0
26-27	37.36247768586091	38.0	38.0	38.0	37.0	38.0
28-29	37.34593824663959	38.0	38.0	38.0	37.0	38.0
30-31	37.34078296983881	38.0	38.0	38.0	37.0	38.0
32-33	37.3270817065088	38.0	38.0	38.0	37.0	38.0
34-35	37.30522258355068	38.0	38.0	38.0	37.0	38.0
36-37	37.28357994804057	38.0	38.0	38.0	37.0	38.0
38-39	37.28118299613034	38.0	38.0	38.0	37.0	38.0
40-41	37.27134347647207	38.0	38.0	38.0	37.0	38.0
42-43	37.23065149253293	38.0	38.0	38.0	37.0	38.0
44-45	37.146036107338006	38.0	38.0	38.0	36.0	38.0
46-47	37.17368586182575	38.0	38.0	38.0	37.0	38.0
48-49	37.132490463491955	38.0	38.0	38.0	36.0	38.0
50-51	37.092442315291365	38.0	38.0	38.0	36.0	38.0
52-53	37.07159463188371	38.0	38.0	38.0	36.0	38.0
54-55	37.041667906270035	38.0	38.0	38.0	36.0	38.0
56-57	36.99602211807881	38.0	38.0	38.0	36.0	38.0
58-59	36.981230643333696	38.0	38.0	38.0	36.0	38.0
60-61	36.98532444734765	38.0	38.0	38.0	36.0	38.0
62-63	36.98881923326765	38.0	38.0	38.0	36.0	38.0
64-65	36.95139685746045	38.0	38.0	38.0	36.0	38.0
66-67	36.97232545261686	38.0	38.0	38.0	36.0	38.0
68-69	36.95410715706395	38.0	38.0	38.0	36.0	38.0
70-71	36.95612757847418	38.0	38.0	38.0	36.0	38.0
72-73	36.93603180360975	38.0	38.0	38.0	36.0	38.0
74-75	36.926902890893324	38.0	38.0	38.0	35.5	38.0
76-77	36.9104837517977	38.0	38.0	38.0	35.0	38.0
78-79	36.88730180154542	38.0	38.0	38.0	35.0	38.0
80-81	36.84895520788412	38.0	38.0	38.0	35.0	38.0
82-83	36.81235688771825	38.0	38.0	38.0	35.0	38.0
84-85	36.787497785977735	38.0	38.0	38.0	35.0	38.0
86-87	36.77569851305347	38.0	38.0	38.0	35.0	38.0
88-89	36.7560399553588	38.0	38.0	38.0	35.0	38.0
90-91	36.73039253703598	38.0	38.0	38.0	35.0	38.0
92-93	36.70127874291851	38.0	38.0	38.0	34.0	38.0
94-95	36.6714570928487	38.0	38.0	38.0	34.0	38.0
96-97	36.671734878448405	38.0	38.0	38.0	34.0	38.0
98-99	36.677449974859485	38.0	38.0	38.0	34.0	38.0
100	35.230836989000444	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	19.0
21	84.0
22	357.0
23	1210.0
24	2923.0
25	6423.0
26	11377.0
27	19376.0
28	29718.0
29	43328.0
30	58491.0
31	76632.0
32	100755.0
33	134639.0
34	196899.0
35	337161.0
36	873684.0
37	4904097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.971374002564005	19.07752011202296	13.361824568960825	25.58928131645221
2	14.23103518285847	20.389874445716234	43.17268054129285	22.20640983013244
3	18.257007759745804	25.825992963661477	30.27105630851018	25.64594296808254
4	12.247716956613019	17.45642345344251	40.012934781601246	30.282924808343225
5	13.33606387827419	36.97235823454969	36.085960967919334	13.605616919256786
6	29.585495696361196	38.07419427687025	19.176273014224336	13.164037012544219
7	26.073938331466383	32.46467872402341	24.12293748964644	17.33844545486377
8	23.62779082731098	37.39769651464181	21.720102077656346	17.25441058039086
9	25.56384428949779	16.758984390286674	21.732695539237017	35.94447578097852
10-11	24.050813738496604	26.546776217040087	29.533699833497067	19.868710210966242
12-13	24.812488359139632	24.238882665718037	29.604925750068634	21.3437032250737
14-15	22.023978186241408	25.4571897337395	27.09088242208752	25.42794965793157
16-17	21.644548664166216	28.479863260900334	27.827055581007293	22.048532493926157
18-19	21.661195278393475	27.781573470637372	29.448375487592056	21.108855763377097
20-21	22.357050885200522	27.019808730306316	29.26268304854538	21.36045733594778
22-23	22.172248441292517	26.958020184944676	29.096061842921717	21.773669530841094
24-25	21.925989553825783	27.01433003463053	29.203999523575476	21.855680887968212
26-27	21.869783450662776	27.1477026581593	29.14455212723377	21.837961763944154
28-29	21.847046235179732	27.21003299521693	29.170890760463468	21.772030009139865
30-31	22.074006111612725	27.091948643360748	29.062761810773356	21.77128343425317
32-33	21.87426299454081	27.210890929833393	29.061510715450584	21.853335360175212
34-35	22.001927462044307	27.17720003224764	29.066438270550286	21.754434235157767
36-37	22.005926415223872	27.1428852401831	29.021043041657467	21.83014530293556
38-39	21.869106248913997	27.295182742235102	29.07953051356672	21.756180495284177
40-41	22.058443794597018	27.11079199898723	28.988396473834378	21.842367732581373
42-43	21.97001224798682	27.248143458084144	28.792476829790726	21.98936746413831
44-45	22.040371196329936	27.379750459084924	28.641563182840844	21.938315161744296
46-47	22.068179828901247	27.29242637616862	28.536049338394548	22.103344456535584
48-49	22.029490443186734	27.460603394819483	28.430548506105218	22.07935765588856
50-51	22.003822735521616	27.526352597331567	28.374656441539976	22.09516822560684
52-53	22.15467520473805	27.449733627431293	28.26960417179143	22.12598699603922
54-55	22.124666361883687	27.336858661755976	28.301851876894563	22.236623099465774
56-57	22.136789828546828	27.43383933299498	28.303701589381298	22.12566924907689
58-59	22.112678471087122	27.469742992619476	28.318833167480317	22.09874536881308
60-61	22.17179870909744	27.4304615070836	28.37350109740358	22.024238686415384
62-63	22.1342117837863	27.477687482948948	28.348277667897783	22.03982306536697
64-65	22.088668528643396	27.3810413466678	28.304438232350286	22.225851892338515
66-67	22.109249222277455	27.369090733908035	28.21600899894414	22.305651044870363
68-69	22.037917524627616	27.35867004968457	28.381614857891364	22.221797567796454
70-71	22.063139218348844	27.182805868996734	28.42966785418262	22.3243870584718
72-73	22.198482420908654	27.314172349181703	28.310215741089383	22.177129488820256
74-75	22.061378710151907	27.426955628573875	28.294693323780585	22.216972337493633
76-77	22.210578196845844	27.392759577926572	28.108428953976244	22.28823327125134
78-79	22.100523893941396	27.393949411632462	28.13684188054743	22.368684813878712
80-81	22.131892063881086	27.49108433096024	28.111051631514584	22.26597197364409
82-83	22.194542431527626	27.46246150478685	28.175442862558047	22.167553201127475
84-85	22.27749098258988	27.4743625974279	28.04346116583823	22.204685254143993
86-87	22.13046587343673	27.51290069289918	28.115874949046088	22.240758484618002
88-89	22.240324017669437	27.489213831551034	28.00017772331753	22.270284427461995
90-91	22.286574270171013	27.522772178826738	27.97878840224421	22.211865148758044
92-93	22.276781693770754	27.515966405877247	28.015356108814913	22.191895791537085
94-95	22.156588448397603	27.596586461729427	28.039561082140484	22.207264007732487
96-97	22.265790564138992	27.6619507093668	28.062027664318556	22.010231062175656
98-99	23.323910643867173	29.629365911356974	28.256261569304808	18.790461875471046
100	29.71665464858568	40.68781607753954	29.59552927387478	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	972.0
1	964.0
2	1592.0
3	2391.5
4	3017.0
5	4372.0
6	5631.0
7	6850.0
8	8086.5
9	8391.0
10	7838.0
11	7480.5
12	8208.0
13	10242.0
14	14320.0
15	18988.5
16	22393.5
17	24394.5
18	24646.5
19	23257.0
20	21576.5
21	21104.0
22	22843.5
23	26992.5
24	33993.5
25	44100.0
26	56792.0
27	70928.5
28	85544.5
29	101723.0
30	117508.0
31	132756.5
32	150304.0
33	167785.0
34	183214.5
35	198307.0
36	214207.5
37	229001.0
38	239521.5
39	246449.0
40	255017.0
41	263694.0
42	269050.0
43	273816.5
44	278843.0
45	282042.5
46	282851.0
47	282987.0
48	281772.0
49	279049.0
50	274536.5
51	266781.0
52	254831.5
53	240525.0
54	225474.0
55	209027.5
56	190345.5
57	170871.0
58	151228.5
59	128633.5
60	106027.0
61	85686.0
62	67825.0
63	52370.5
64	39086.5
65	29176.5
66	21837.0
67	16491.5
68	12406.5
69	9024.5
70	6378.5
71	4452.0
72	3135.5
73	2064.5
74	1344.0
75	891.0
76	568.5
77	366.0
78	229.0
79	133.5
80	84.0
81	58.5
82	42.5
83	31.0
84	21.5
85	16.5
86	16.0
87	13.5
88	9.5
89	7.0
90	4.5
91	2.5
92	1.5
93	1.5
94	1.5
95	1.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0017360145636909907
3	0.002604021845536486
4	1.471198782788975E-5
5	0.0
6	3.383757200414643E-4
7	0.0
8	0.0
9	0.0
10-11	7.355993913944875E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.059759053799656E-4
24-25	9.78441044733736E-4
26-27	0.0029208880058700284
28-29	0.0017883466036501404
30-31	0.002268637631542313
32-33	0.0030549280490653912
34-35	7.470153961352382E-4
36-37	0.0
38-39	2.9842949241395963E-5
40-41	7.504203854999571E-6
42-43	5.208669883863269E-4
44-45	0.0
46-47	0.0
48-49	2.0095240620024736E-4
50-51	0.005773038259661912
52-53	0.0
54-55	0.0029971273443822684
56-57	0.04361767378573021
58-59	0.054583765203408796
60-61	0.034446407914946915
62-63	0.008097057935227303
64-65	0.0
66-67	0.0
68-69	4.63002131072536E-4
70-71	0.0024495371948189226
72-73	0.009734614476187954
74-75	0.0048842942784700135
76-77	0.003724430361521254
78-79	0.003383675396047247
80-81	0.015297531616092859
82-83	0.04963227653680988
84-85	0.03295448638588944
86-87	0.00947072606895965
88-89	0.008099785617567967
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	47.0
20-21	151.0
22-23	356.0
24-25	530.0
26-27	1379.0
28-29	3756.0
30-31	11735.0
32-33	14553.0
34-35	20408.0
36-37	32819.0
38-39	38966.0
40-41	38820.0
42-43	43156.0
44-45	52796.0
46-47	55224.0
48-49	52408.0
50-51	48491.0
52-53	46394.0
54-55	51090.0
56-57	55152.0
58-59	55054.0
60-61	53816.0
62-63	51657.0
64-65	54850.0
66-67	58952.0
68-69	60687.0
70-71	60415.0
72-73	56208.0
74-75	57005.0
76-77	60051.0
78-79	61984.0
80-81	61839.0
82-83	58588.0
84-85	57020.0
86-87	59468.0
88-89	62721.0
90-91	64171.0
92-93	63439.0
94-95	71737.0
96-97	211925.0
98-99	1570136.0
100-101	3317224.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.33507798618864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.84382005936341	76.66829391342877
2	7.0702266317638855	12.06676682003198
3	1.7445249312956577	4.466075131828959
4	0.6185354011630951	2.111310667818848
5	0.2775695442938172	1.1843209354451867
6	0.15538066037694298	0.7955652462487152
7	0.0948586025476821	0.5666336372247126
8	0.05324138501918416	0.3634686194163821
9	0.03427286677904395	0.2632209983459972
>10	0.10570007678228012	1.341723221627806
>50	0.0014531493104063984	0.08235839979033108
>100	3.6247404806522E-4	0.05924083049766168
>500	5.421725647610671E-5	0.03102157829464727
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.471198782788975E-5	0.0	0.0	0.0
3	0.0	2.94239756557795E-5	0.0	0.0	0.0
4	0.0	2.94239756557795E-5	0.0	0.0	2.94239756557795E-5
5	0.0	4.4135963483669255E-5	0.0	0.0	2.94239756557795E-5
6	0.0	4.4135963483669255E-5	0.0	0.0	4.4135963483669255E-5
7	1.471198782788975E-5	4.4135963483669255E-5	0.0	0.0	4.4135963483669255E-5
8	1.471198782788975E-5	5.8847951311559E-5	0.0	0.0	4.4135963483669255E-5
9	1.471198782788975E-5	1.3240789045100776E-4	0.0	0.0	4.4135963483669255E-5
10-11	1.471198782788975E-5	1.471198782788975E-4	0.0	0.0	4.4135963483669255E-5
12-13	1.471198782788975E-5	1.471198782788975E-4	0.0	0.0	5.149195739761413E-5
14-15	1.471198782788975E-5	2.1332382350440138E-4	0.0	0.0	1.3240789045100776E-4
16-17	1.471198782788975E-5	2.35391805246236E-4	0.0	0.0	1.9125584176256675E-4
18-19	1.471198782788975E-5	2.5745978698807066E-4	0.0	7.355993913944875E-6	1.9125584176256675E-4
20-21	1.471198782788975E-5	2.94239756557795E-4	0.0	3.6779969569724374E-5	1.9125584176256675E-4
22-23	1.471198782788975E-5	3.236637322135745E-4	0.0	5.8847951311559E-5	2.059678295904565E-4
24-25	1.471198782788975E-5	3.604437017832989E-4	0.0	6.620394522550388E-5	2.2067981741834627E-4
26-27	1.471198782788975E-5	3.825116835251335E-4	0.0	1.1033990870917314E-4	2.2067981741834627E-4
28-29	1.471198782788975E-5	3.8986767743907837E-4	0.0	3.6779969569724377E-4	2.2067981741834627E-4
30-31	1.471198782788975E-5	4.6342761657852715E-4	0.0	0.0012799429410264083	2.2067981741834627E-4
32-33	1.471198782788975E-5	4.8549559832036175E-4	0.0	0.003597081023919044	2.2067981741834627E-4
34-35	1.471198782788975E-5	5.590555374598105E-4	0.0	0.006598326540808553	2.6481578090201553E-4
36-37	1.471198782788975E-5	6.546834583410939E-4	0.0	0.01129880665181933	2.6481578090201553E-4
38-39	1.471198782788975E-5	7.061754157387081E-4	0.0	0.018294356863980905	2.6481578090201553E-4
40-41	1.471198782788975E-5	7.429553853084324E-4	0.0	0.025760690686634953	2.721717748159604E-4
42-43	1.471198782788975E-5	8.018033366199915E-4	0.0	0.0332417364971169	2.8688376264385013E-4
44-45	1.471198782788975E-5	8.753632757594401E-4	0.0	0.041127361972865796	3.089517443856848E-4
46-47	1.471198782788975E-5	9.121432453291646E-4	0.0	0.05097703782363799	3.1630773829962965E-4
48-49	1.471198782788975E-5	9.268552331570543E-4	0.0	0.06127542930316081	3.236637322135745E-4
50-51	1.471198782788975E-5	0.001000415172296503	0.0	0.07150026084354419	3.236637322135745E-4
52-53	1.471198782788975E-5	0.001000415172296503	0.0	0.08097478100470519	3.4573171395540917E-4
54-55	1.471198782788975E-5	0.0010298391479522826	0.0	0.08933119009094656	3.6779969569724377E-4
56-57	1.471198782788975E-5	0.0010739751114359518	0.0	0.09531161314298375	3.6779969569724377E-4
58-59	1.471198782788975E-5	0.0010886870992638416	0.0	0.10199821161075964	3.6779969569724377E-4
60-61	1.471198782788975E-5	0.001184315020145125	0.0	0.1085303342063427	3.6779969569724377E-4
62-63	1.471198782788975E-5	0.0012137389958009046	0.0	0.11456960520969144	3.972236713530233E-4
64-65	1.471198782788975E-5	0.0012652309531985186	0.0	0.12088104798785614	3.972236713530233E-4
66-67	1.471198782788975E-5	0.0012799429410264083	0.0	0.12725869471124635	4.0457966526696816E-4
68-69	1.471198782788975E-5	0.001302010922768243	0.0	0.1332023377937138	4.11935659180913E-4
70-71	1.471198782788975E-5	0.0013240789045100775	0.0	0.13855750136306566	4.2664764700880276E-4
72-73	1.471198782788975E-5	0.0013387908923379673	0.0	0.14268421394878875	4.8549559832036175E-4
74-75	1.471198782788975E-5	0.0013829268558216367	0.0	0.14603119117963367	4.8549559832036175E-4
76-77	1.471198782788975E-5	0.0014491308010471404	0.0	0.14986366400879894	4.8549559832036175E-4
78-79	1.471198782788975E-5	0.0014564867949610853	0.0	0.15223965004300316	4.8549559832036175E-4
80-81	1.471198782788975E-5	0.0014638427888750303	0.0	0.1530193853978813	4.8549559832036175E-4
82-83	1.471198782788975E-5	0.001471198782788975	0.0	0.15351223699011562	4.8549559832036175E-4
84-85	1.471198782788975E-5	0.0015006227584447546	0.0	0.1537917647588455	4.8549559832036175E-4
86-87	1.471198782788975E-5	0.0015153347462726443	0.0	0.15389474867364072	4.8549559832036175E-4
88	1.471198782788975E-5	0.0015741826975842034	0.0	0.1539021046675547	4.8549559832036175E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7230	0.0	46.894005	1
GTATCAA	13075	0.0	37.379322	1
TCAACGC	14915	0.0	31.737465	4
ATCAACG	15095	0.0	31.302969	3
CAACGCA	15250	0.0	30.984354	5
AACGCAG	15815	0.0	29.904383	6
TATCAAC	16115	0.0	29.639864	2
ACGCAGA	18210	0.0	25.994745	7
CGCAGAG	18695	0.0	25.274748	8
GTACATG	16775	0.0	23.058617	1
TACATGG	16710	0.0	22.612354	2
ACATGGG	16665	0.0	21.956387	3
GCAGAGT	22600	0.0	21.001932	9
CATGGGG	12440	0.0	19.9172	4
GAGTACT	14460	0.0	19.051832	12-13
CAGAGTA	21985	0.0	18.34035	10-11
AGAGTAC	20235	0.0	18.314249	10-11
GTACTTT	16655	0.0	16.361893	14-15
TGCGCGA	495	9.128553E-6	15.89791	94
AGTACTT	16280	0.0	15.730086	12-13
>>END_MODULE