FastQCFastQC Report
Fri 27 May 2016
SRR2138682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7197428
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70600.045.4998861
GTATCAA127000.037.754721
TCAACGC141600.032.800034
ATCAACG143950.032.0571373
CAACGCA146100.031.7310545
AACGCAG150800.030.7989676
TATCAAC155450.030.044872
ACGCAGA176100.026.4713577
CGCAGAG180900.025.6978478
GTACATG186150.022.3942411
TACATGG185450.021.8543622
ACATGGG188350.020.8115633
GCAGAGT224900.020.727469
GAGTACT129950.018.1506612-13
AGAGTAC197350.017.96022410-11
CAGAGTA217900.017.92945910-11
CATGGGG135650.017.2616334
GTACTTT151350.015.58437214-15
GAGTACA133550.015.5429931
AGTACTT144150.015.23215412-13
Produced by FastQC (version 0.11.5)