FastQCFastQC Report
Fri 27 May 2016
SRR2138681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6862002
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74000.046.736811
GTATCAA134050.036.9528271
TCAACGC152600.031.511644
ATCAACG152850.031.4324653
CAACGCA154750.030.9912665
AACGCAG161400.029.7143656
TATCAAC164400.029.5609662
ACGCAGA187600.025.587017
CGCAGAG187800.025.51448
GTACATG177950.022.068241
TACATGG177650.021.6020182
GCAGAGT226450.021.2348929
ACATGGG177200.020.5511993
GAGTACT132400.019.30152712-13
CAGAGTA220550.018.68410710-11
AGAGTAC202000.018.25975410-11
CATGGGG132900.018.1714554
GTACTTT156850.016.33368914-15
AGTACTT150200.015.17093312-13
CGTCGAA3500.00366932915.05267994
Produced by FastQC (version 0.11.5)