##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138681_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6862002
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.808781897761033	33.0	32.0	34.0	28.0	34.0
2	32.18071183890649	33.0	33.0	34.0	29.0	34.0
3	32.00084129966736	33.0	31.0	33.0	29.0	34.0
4	32.56946413014744	33.0	33.0	34.0	31.0	34.0
5	32.86909622002442	33.0	33.0	34.0	31.0	34.0
6	36.60384549581886	38.0	37.0	38.0	34.0	38.0
7	37.06488048240149	38.0	38.0	38.0	36.0	38.0
8	37.01816743859882	38.0	38.0	38.0	36.0	38.0
9	37.124299584873334	38.0	38.0	38.0	36.0	38.0
10-11	37.22741118408301	38.0	38.0	38.0	36.0	38.0
12-13	37.30477679837459	38.0	38.0	38.0	37.0	38.0
14-15	37.264313679885255	38.0	38.0	38.0	37.0	38.0
16-17	37.331159040758074	38.0	38.0	38.0	37.0	38.0
18-19	37.34973780246639	38.0	38.0	38.0	37.0	38.0
20-21	37.36215162598303	38.0	38.0	38.0	37.0	38.0
22-23	37.37264927625692	38.0	38.0	38.0	37.0	38.0
24-25	37.38615478518237	38.0	38.0	38.0	37.0	38.0
26-27	37.35459309041012	38.0	38.0	38.0	37.0	38.0
28-29	37.33567400569506	38.0	38.0	38.0	37.0	38.0
30-31	37.332424165503774	38.0	38.0	38.0	37.0	38.0
32-33	37.317622180564484	38.0	38.0	38.0	37.0	38.0
34-35	37.297575188620776	38.0	38.0	38.0	37.0	38.0
36-37	37.27672565592289	38.0	38.0	38.0	37.0	38.0
38-39	37.278154378924036	38.0	38.0	38.0	37.0	38.0
40-41	37.26769255574454	38.0	38.0	38.0	37.0	38.0
42-43	37.22671703680987	38.0	38.0	38.0	37.0	38.0
44-45	37.14668319481578	38.0	38.0	38.0	36.0	38.0
46-47	37.17777371027407	38.0	38.0	38.0	37.0	38.0
48-49	37.139320445370245	38.0	38.0	38.0	36.0	38.0
50-51	37.09925671110376	38.0	38.0	38.0	36.0	38.0
52-53	37.07664049108445	38.0	38.0	38.0	36.0	38.0
54-55	37.04592988028406	38.0	38.0	38.0	36.0	38.0
56-57	36.999174568600296	38.0	38.0	38.0	36.0	38.0
58-59	36.981699860008845	38.0	38.0	38.0	36.0	38.0
60-61	36.987752864408	38.0	38.0	38.0	36.0	38.0
62-63	36.98778345712813	38.0	38.0	38.0	36.0	38.0
64-65	36.94889975785668	38.0	38.0	38.0	36.0	38.0
66-67	36.968805763131556	38.0	38.0	38.0	36.0	38.0
68-69	36.95233943963917	38.0	38.0	38.0	36.0	38.0
70-71	36.95092361562706	38.0	38.0	38.0	36.0	38.0
72-73	36.93037470997137	38.0	38.0	38.0	36.0	38.0
74-75	36.91872775031803	38.0	38.0	38.0	35.5	38.0
76-77	36.90107638929301	38.0	38.0	38.0	35.0	38.0
78-79	36.87681117891023	38.0	38.0	38.0	35.0	38.0
80-81	36.8392571226629	38.0	38.0	38.0	35.0	38.0
82-83	36.798591891446804	38.0	38.0	38.0	35.0	38.0
84-85	36.77507611867158	38.0	38.0	38.0	35.0	38.0
86-87	36.76066931665276	38.0	38.0	38.0	35.0	38.0
88-89	36.74128538157128	38.0	38.0	38.0	35.0	38.0
90-91	36.712821967028205	38.0	38.0	38.0	34.5	38.0
92-93	36.685056410536404	38.0	38.0	38.0	34.0	38.0
94-95	36.65244217286126	38.0	38.0	38.0	34.0	38.0
96-97	36.65405814853715	38.0	38.0	38.0	34.0	38.0
98-99	36.660963749195076	38.0	38.0	38.0	34.0	38.0
100	35.20723886848341	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	13.0
21	96.0
22	406.0
23	1305.0
24	3228.0
25	6767.0
26	12202.0
27	19981.0
28	30481.0
29	43892.0
30	58746.0
31	77009.0
32	100949.0
33	136646.0
34	200984.0
35	344011.0
36	889784.0
37	4935499.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.88847511265663	19.071562497358645	13.246075999394929	25.793886390589805
2	14.24902070827286	20.290018124857635	43.17576594173551	22.28519522513399
3	18.36189113230246	25.858901762871778	29.952964339609856	25.826242765215905
4	12.2490946882695	17.302008554064624	39.919944634225494	30.528952123440376
5	13.411873094761559	37.05469628251347	35.902787553836326	13.630643068888643
6	29.71952502831914	37.96168066678257	19.129485758238125	13.189308546660165
7	26.213749281915106	32.34386990851941	24.028672681820844	17.41370812774464
8	23.73932563703712	37.46578622390375	21.66080686073831	17.134081278320817
9	25.740374310587494	16.611085219736164	21.617379301259312	36.03116116841703
10-11	24.274253238960473	26.51544352733263	29.30230555198112	19.907997681725785
12-13	24.940928318003987	24.12025674139996	29.497841883462	21.440973057134055
14-15	22.13048028840563	25.368595054329624	26.86123524883846	25.639689408426285
16-17	21.800751442509053	28.32556737814999	27.641867489983245	22.23181368935771
18-19	21.847224760354194	27.760287741099464	29.16449893194435	21.22798856660199
20-21	22.56516472206283	26.966028180816277	28.93827727227186	21.530529824849033
22-23	22.328251547696766	26.87644228418539	28.80397410069489	21.991332067422952
24-25	22.108215943350825	26.917561611340563	28.92514965783542	22.049072787473197
26-27	22.062561875631875	27.123901106269248	28.79000999338827	22.023527024710607
28-29	22.04705369483697	27.216362724457483	28.822447497798436	21.914136082907117
30-31	22.26468014839788	27.06546019453291	28.721963808754445	21.947895848314765
32-33	22.029464251646516	27.162807438266746	28.77557414035554	22.0321541697312
34-35	22.158212631260138	27.138022539552132	28.779707003551845	21.92405782563588
36-37	22.181568078325697	27.09138419665641	28.68719003379819	22.039857691219705
38-39	22.04301734899545	27.278876620578586	28.757964772810425	21.920141257615548
40-41	22.292478513912105	27.108787512297393	28.604769093855214	21.99396487993529
42-43	22.16281254924237	27.17853494205094	28.523636413699233	22.135016095007458
44-45	22.21960652455139	27.299232790921867	28.380775214921556	22.10038546960519
46-47	22.213042951917554	27.25349587202616	28.205043637082404	22.32841753897388
48-49	22.230692791680777	27.350998762137134	28.128538796881287	22.289769649300798
50-51	22.192667590372338	27.442991569962206	28.057915263785453	22.30642557588
52-53	22.289150153355962	27.40796111711973	27.96863892009995	22.334249809424364
54-55	22.279124760327374	27.31664970444462	27.989431768046245	22.414793767181752
56-57	22.277062943362267	27.38677785138482	27.994773433801274	22.341385771451634
58-59	22.25726675489837	27.492387958472786	28.001537465046994	22.248807821581845
60-61	22.382966652617803	27.388442486020303	28.005112389566666	22.22347847179523
62-63	22.341391840152173	27.436695741239014	27.985335360153474	22.236577058455335
64-65	22.227492301630157	27.377639824680994	28.030215947903447	22.364651925785402
66-67	22.250758450696008	27.401454368463344	27.924274998256077	22.423512182584567
68-69	22.23997321639072	27.3250898184426	28.08414539535116	22.35079156981552
70-71	22.27523667131262	27.11939735742192	28.115568139930897	22.48979783133456
72-73	22.381176481830085	27.219485992216306	28.031555906229823	22.36778161972379
74-75	22.24316712415145	27.382025564304215	27.96855651742455	22.40625079411979
76-77	22.35514320509432	27.306042849036917	27.87546847483438	22.463345471034383
78-79	22.276574776147438	27.30837735116416	27.86663705179367	22.548410820894734
80-81	22.307337848936328	27.418896457576675	27.865260718984636	22.408504974502364
82-83	22.400358931907725	27.373559120548464	27.935100822321417	22.290981125222388
84-85	22.47375087766683	27.397673090076108	27.758663841653362	22.3699121906037
86-87	22.301758560652694	27.417581383521007	27.856572030021898	22.424088025804405
88-89	22.38582977774259	27.38611334629549	27.789198386508858	22.438858489453057
90-91	22.4039295902394	27.508305691524836	27.72467209036878	22.363092627866983
92-93	22.497365633025193	27.457570887880294	27.715327714301058	22.329735764793448
94-95	22.321735170294087	27.528782071616213	27.783457113115677	22.366025644974023
96-97	22.38366141185138	27.61118085952226	27.85591361479851	22.14924411382784
98-99	23.470688680870396	29.519336446477308	28.0933133794916	18.916661493160696
100	30.089477429107514	40.61574263322724	29.29477993766525	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	808.0
1	830.0
2	1376.5
3	2092.0
4	2911.5
5	4522.0
6	5811.0
7	6708.0
8	7514.0
9	7559.0
10	6969.5
11	6646.5
12	7408.0
13	9353.0
14	13317.5
15	18262.0
16	21837.5
17	23834.0
18	24021.0
19	22463.0
20	20631.5
21	19708.0
22	21034.0
23	25380.5
24	32845.5
25	43111.5
26	55744.0
27	70172.5
28	84629.0
29	100450.5
30	115966.5
31	130708.5
32	146138.0
33	161582.0
34	176382.0
35	191408.0
36	207958.5
37	222919.5
38	234315.5
39	242386.0
40	251245.0
41	260855.0
42	268312.0
43	274839.0
44	282081.0
45	288921.0
46	291490.5
47	292050.5
48	291382.5
49	287383.0
50	281888.5
51	275003.0
52	265161.5
53	252598.5
54	237849.0
55	220839.5
56	201732.0
57	181383.0
58	160699.5
59	137042.5
60	112799.0
61	91193.5
62	72287.5
63	55191.5
64	40485.0
65	29811.5
66	21738.0
67	16160.0
68	11944.0
69	8471.0
70	6061.0
71	4342.0
72	2960.5
73	1862.5
74	1169.5
75	767.0
76	492.0
77	289.5
78	174.5
79	110.5
80	70.5
81	44.0
82	26.5
83	17.5
84	12.5
85	10.0
86	9.0
87	7.0
88	5.5
89	5.0
90	4.5
91	4.0
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0022588160131693344
3	0.002725152222339778
4	1.4573006536576352E-5
5	0.0
6	5.100552287801723E-4
7	0.0
8	0.0
9	0.0
10-11	1.4573006536576352E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.7689739384901586E-4
24-25	0.0011513690287852458
26-27	0.003046316398583536
28-29	0.0017932949140552477
30-31	0.002684911613877024
32-33	0.0032965028928457485
34-35	9.450794548078389E-4
36-37	7.35126714159283E-6
38-39	5.912830976257175E-5
40-41	7.436626187127231E-6
42-43	5.985741664069104E-4
44-45	0.0
46-47	0.0
48-49	2.3756365748102518E-4
50-51	0.006351682948713947
52-53	0.0
54-55	0.002824559766860837
56-57	0.0467993441920471
58-59	0.05829190353240793
60-61	0.036862659044731905
62-63	0.008721610973395999
64-65	0.0
66-67	0.0
68-69	6.769401103562811E-4
70-71	0.002778206096279281
72-73	0.010185882980413247
74-75	0.004858161914439671
76-77	0.003775670207560945
78-79	0.003912738372594512
80-81	0.01641239895394517
82-83	0.052978777383231375
84-85	0.03432241484443534
86-87	0.009179604937342312
88-89	0.008139750513398289
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	36.0
20-21	151.0
22-23	310.0
24-25	538.0
26-27	1337.0
28-29	3682.0
30-31	11997.0
32-33	14625.0
34-35	20130.0
36-37	33790.0
38-39	41924.0
40-41	40476.0
42-43	44864.0
44-45	54335.0
46-47	55528.0
48-49	54734.0
50-51	50470.0
52-53	47727.0
54-55	52000.0
56-57	56087.0
58-59	56123.0
60-61	56285.0
62-63	53084.0
64-65	54760.0
66-67	59043.0
68-69	61436.0
70-71	61232.0
72-73	56781.0
74-75	56510.0
76-77	59819.0
78-79	62733.0
80-81	62821.0
82-83	58740.0
84-85	56661.0
86-87	58563.0
88-89	62969.0
90-91	63964.0
92-93	63950.0
94-95	71767.0
96-97	215068.0
98-99	1595509.0
100-101	3329443.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.59020576010448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.46576564603063	75.6792752448302
2	7.305526880793607	12.35952044064611
3	1.7761226821192557	4.507277494069695
4	0.6666550448493357	2.2556994965926793
5	0.3048769771134652	1.2894803112773343
6	0.1624269033417659	0.824383510479395
7	0.09685738414041523	0.5735230237676242
8	0.05457246470507624	0.3693036814590751
9	0.038350207801709044	0.29196467720003993
>10	0.1263386963928398	1.6354711027545492
>50	0.0019827052969555903	0.11239883730354394
>100	4.399112510030281E-4	0.05705383294319775
>500	8.44961639083964E-5	0.044648346676552715
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.9146013073152704E-5	0.0	0.0	0.0
3	0.0	2.9146013073152704E-5	0.0	1.4573006536576352E-5	0.0
4	0.0	1.0201104575603447E-4	0.0	2.9146013073152704E-5	0.0
5	0.0	1.4573006536576353E-4	0.0	2.9146013073152704E-5	0.0
6	0.0	1.4573006536576353E-4	0.0	2.9146013073152704E-5	0.0
7	0.0	1.6030307190233986E-4	0.0	4.3719019609729055E-5	0.0
8	0.0	2.0402209151206894E-4	0.0	5.829202614630541E-5	0.0
9	0.0	2.4774111112179796E-4	0.0	8.743803921945811E-5	0.0
10-11	0.0	2.4774111112179796E-4	0.0	1.0201104575603447E-4	0.0
12-13	0.0	2.4774111112179796E-4	0.0	1.3115705882918717E-4	0.0
14-15	0.0	2.7688712419495073E-4	0.0	1.8944908497549258E-4	0.0
16-17	0.0	2.9146013073152706E-4	0.0	1.9673558824378075E-4	0.0
18-19	0.0	3.206061438046797E-4	0.0	2.2588160131693346E-4	0.0
20-21	0.0	3.934711764875615E-4	0.0	2.404546078535098E-4	0.0
22-23	0.0	4.371901960972906E-4	0.0	2.4774111112179796E-4	0.0
24-25	0.0	4.881957189753078E-4	0.0	2.623141176583743E-4	0.0
26-27	0.0	5.100552287801724E-4	0.0	2.9146013073152706E-4	0.0
28-29	0.0	5.319147385850369E-4	0.0	5.902067647313423E-4	0.0
30-31	0.0	6.703583006825122E-4	0.0	0.0015957442157551105	0.0
32-33	0.0	6.703583006825122E-4	0.0	0.0035558135949246296	0.0
34-35	0.0	8.233748693165639E-4	0.0	0.005865635130971982	0.0
36-37	0.0	9.982509477554802E-4	0.0	0.009800346895847595	0.0
38-39	0.0	0.001034683464096921	0.0	0.015651409020283003	0.0
40-41	0.0	0.0011075484967798027	0.0	0.022566300621888483	0.0
42-43	1.4573006536576352E-5	0.0011366945098529556	0.0	0.028701536373787126	0.0
44-45	1.4573006536576352E-5	0.0012532785621455662	0.0	0.03603175866168503	0.0
46-47	1.4573006536576352E-5	0.0013261435948284481	0.0	0.04413435029602149	0.0
48-49	1.4573006536576352E-5	0.0013480031046333126	0.0	0.05205477934865073	0.0
50-51	1.4573006536576352E-5	0.0014645871569259235	0.0	0.05992420287840196	0.0
52-53	1.4573006536576352E-5	0.0014718736601942116	0.0	0.06818709758464075	0.0
54-55	1.4573006536576352E-5	0.0015083061765356525	0.0	0.07525500575488028	0.0
56-57	1.4573006536576352E-5	0.0015447386928770933	0.0	0.08141938751985207	0.0
58-59	1.4573006536576352E-5	0.0015957442157551105	0.0	0.08735060118023866	0.0
60-61	1.4573006536576352E-5	0.0016394632353648397	0.0	0.09351498294521045	0.0
62-63	1.4573006536576352E-5	0.0016758957517062805	0.0	0.09969393771671883	0.0
64-65	1.4573006536576352E-5	0.0017924798039988914	0.0	0.10611334709608071	0.0
66-67	1.4573006536576352E-5	0.0018143393138037559	0.0	0.11254004297871087	0.0
68-69	1.4573006536576352E-5	0.0018361988236086204	0.0	0.11888658732538987	0.0
70-71	1.4573006536576352E-5	0.0018507718301451967	0.0	0.12455548686811808	0.0
72-73	1.4573006536576352E-5	0.001865344836681773	0.0	0.12895653484216413	0.0
74-75	1.4573006536576352E-5	0.0019017773530232139	0.0	0.1324759159207473	0.0
76-77	1.4573006536576352E-5	0.001981928888974384	0.0	0.13566011784898924	0.0
78-79	1.4573006536576352E-5	0.0020110749020475368	0.0	0.13783149582293913	0.0
80-81	1.4573006536576352E-5	0.0020110749020475368	0.0	0.13852371363342653	0.0
82-83	1.4573006536576352E-5	0.002025647908584113	0.0	0.1389609038295238	0.0
84-85	1.4573006536576352E-5	0.0020912264379987066	0.0	0.1391503529144993	0.0
86-87	1.4573006536576352E-5	0.0021422319608767238	0.0	0.13928879647659675	0.0
88	1.4573006536576352E-5	0.0021859509804864528	0.0	0.13930336948313335	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7400	0.0	46.73681	1
GTATCAA	13405	0.0	36.952827	1
TCAACGC	15260	0.0	31.51164	4
ATCAACG	15285	0.0	31.432465	3
CAACGCA	15475	0.0	30.991266	5
AACGCAG	16140	0.0	29.714365	6
TATCAAC	16440	0.0	29.560966	2
ACGCAGA	18760	0.0	25.58701	7
CGCAGAG	18780	0.0	25.5144	8
GTACATG	17795	0.0	22.06824	1
TACATGG	17765	0.0	21.602018	2
GCAGAGT	22645	0.0	21.234892	9
ACATGGG	17720	0.0	20.551199	3
GAGTACT	13240	0.0	19.301527	12-13
CAGAGTA	22055	0.0	18.684107	10-11
AGAGTAC	20200	0.0	18.259754	10-11
CATGGGG	13290	0.0	18.171455	4
GTACTTT	15685	0.0	16.333689	14-15
AGTACTT	15020	0.0	15.170933	12-13
CGTCGAA	350	0.003669329	15.052679	94
>>END_MODULE