FastQCFastQC Report
Fri 27 May 2016
SRR2138680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5317679
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56850.045.53571
GTATCAA104850.035.8319851
TCAACGC121350.030.4710564
ATCAACG123250.030.001323
CAACGCA124100.029.829695
TATCAAC129450.028.9577352
AACGCAG128600.028.851236
ACGCAGA148100.024.995717
CGCAGAG151750.024.4498758
GCAGAGT185700.020.093049
CGTCGAA2705.6201464E-419.91925694
TATACCG4700.019.6684195
GTACATG141850.019.4050061
TACATGG140600.019.2487412
ACATGGG140850.018.61543
GAGTACT107900.018.32241812-13
AGAGTAC165600.018.04072210-11
CAGAGTA181100.017.54074310-11
CATGGGG102200.016.898014
GTACTTT123250.016.07464614-15
Produced by FastQC (version 0.11.5)