##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138680_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5317679
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.68149713437009	30.0	18.0	33.0	18.0	33.0
2	31.417115625068757	33.0	32.0	33.0	27.0	34.0
3	31.668815097714624	33.0	31.0	33.0	29.0	34.0
4	32.72796439950587	33.0	33.0	33.0	32.0	34.0
5	32.704160781423624	33.0	33.0	33.0	32.0	34.0
6	37.01389421963981	38.0	37.0	38.0	35.0	38.0
7	37.02884867627399	38.0	38.0	38.0	35.0	38.0
8	37.38009345806695	38.0	38.0	38.0	37.0	38.0
9	37.464055276747615	38.0	38.0	38.0	37.0	38.0
10-11	37.46390793803086	38.0	38.0	38.0	37.0	38.0
12-13	37.503567345828884	38.0	38.0	38.0	37.5	38.0
14-15	37.50839623828365	38.0	38.0	38.0	37.5	38.0
16-17	37.54292314372492	38.0	38.0	38.0	38.0	38.0
18-19	37.53682189165612	38.0	38.0	38.0	38.0	38.0
20-21	37.54397299126066	38.0	38.0	38.0	38.0	38.0
22-23	37.554505422012525	38.0	38.0	38.0	38.0	38.0
24-25	37.56420468090205	38.0	38.0	38.0	38.0	38.0
26-27	37.52974284653969	38.0	38.0	38.0	38.0	38.0
28-29	37.5031303176604	38.0	38.0	38.0	38.0	38.0
30-31	37.50695129395129	38.0	38.0	38.0	38.0	38.0
32-33	37.49774617396106	38.0	38.0	38.0	38.0	38.0
34-35	37.397180239698024	38.0	38.0	38.0	37.0	38.0
36-37	37.43748624953112	38.0	38.0	38.0	37.0	38.0
38-39	37.44726017658952	38.0	38.0	38.0	37.0	38.0
40-41	37.445999573659634	38.0	38.0	38.0	37.0	38.0
42-43	37.412603350869276	38.0	38.0	38.0	37.0	38.0
44-45	37.38495024657416	38.0	38.0	38.0	37.0	38.0
46-47	37.36465754375168	38.0	38.0	38.0	37.0	38.0
48-49	37.31493019302651	38.0	38.0	38.0	37.0	38.0
50-51	37.288655705128946	38.0	38.0	38.0	37.0	38.0
52-53	37.268560139249324	38.0	38.0	38.0	37.0	38.0
54-55	37.24053963783718	38.0	38.0	38.0	37.0	38.0
56-57	37.200554248350244	38.0	38.0	38.0	37.0	38.0
58-59	37.19373564652651	38.0	38.0	38.0	37.0	38.0
60-61	37.19587155254037	38.0	38.0	38.0	37.0	38.0
62-63	37.2026303751686	38.0	38.0	38.0	37.0	38.0
64-65	37.20889798501576	38.0	38.0	38.0	37.0	38.0
66-67	37.207203980155974	38.0	38.0	38.0	37.0	38.0
68-69	37.20028019593349	38.0	38.0	38.0	37.0	38.0
70-71	37.195186153803164	38.0	38.0	38.0	36.0	38.0
72-73	37.18574056241779	38.0	38.0	38.0	36.0	38.0
74-75	37.18251277436946	38.0	38.0	38.0	36.0	38.0
76-77	37.1697301653051	38.0	38.0	38.0	36.0	38.0
78-79	37.15741285711773	38.0	38.0	38.0	36.0	38.0
80-81	37.14906686028175	38.0	38.0	38.0	36.0	38.0
82-83	37.10069973341954	38.0	38.0	38.0	36.0	38.0
84-85	37.09316030666315	38.0	38.0	38.0	36.0	38.0
86-87	37.08100396576371	38.0	38.0	38.0	36.0	38.0
88-89	37.06849611382319	38.0	38.0	38.0	36.0	38.0
90-91	37.06147973344872	38.0	38.0	38.0	36.0	38.0
92-93	37.04510971582698	38.0	38.0	38.0	36.0	38.0
94-95	37.028373876417646	38.0	38.0	38.0	36.0	38.0
96-97	37.013472585437654	38.0	38.0	38.0	36.0	38.0
98-99	37.02875102565828	38.0	38.0	38.0	36.0	38.0
100	35.65286489279671	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	9.0
21	41.0
22	232.0
23	686.0
24	1740.0
25	3586.0
26	6726.0
27	11205.0
28	17807.0
29	26278.0
30	34762.0
31	44570.0
32	56955.0
33	76824.0
34	115485.0
35	213219.0
36	710874.0
37	3996676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.26747383585959	21.5502101574766	13.420855226500134	24.761460780163677
2	14.169478175550621	20.82178497321771	42.29775708074257	22.710979770489097
3	17.802512657407792	26.50752580516496	31.0514801969371	24.638481340490145
4	12.495131804683961	17.85393589947795	39.750312119253536	29.900620176584557
5	13.204162944021256	37.41848652391391	36.01922944201784	13.358121090046993
6	29.3964152405589	38.702693411919	19.01032386497944	12.890567482542666
7	25.64985588637449	32.95503545813879	24.29695361453747	17.098155040949255
8	23.425069471098197	37.85627150491784	21.723782123742332	16.99487690024163
9	25.689027111264146	16.73818596421484	21.814705250166472	35.75808167435455
10-11	24.04965587430155	26.78325449881424	29.532480241849875	19.634609385034334
12-13	24.751700883035625	24.560687096757817	29.65004092951079	21.03757109069577
14-15	22.00934843942254	25.78111615988855	27.125057755460606	25.084477645228304
16-17	21.609023410401416	28.760526914091656	27.822627127361393	21.807822548145538
18-19	21.5832132778229	28.084376661321603	29.425450464384934	20.906959596470564
20-21	22.40409279304353	27.311279063644772	29.191295386675293	21.0933327566364
22-23	22.15313890898765	27.180621317284416	29.054493259746017	21.61174651398192
24-25	21.88429271788034	27.295312776962543	29.16988227284546	21.65051223231166
26-27	21.834176166169545	27.446462904634057	29.10011560032607	21.619245328870328
28-29	21.832596521309004	27.560134463451487	29.064866018683507	21.542402996556003
30-31	22.08406106659462	27.367651738211713	29.005031565111405	21.543255630082264
32-33	21.874589145775257	27.517665138977147	28.96622210804165	21.641523607205947
34-35	21.989232895126786	27.583898353774654	28.93781773082465	21.489051020273912
36-37	21.98225964832772	27.450590313761523	28.964615772566262	21.602534265344495
38-39	21.81835870164546	27.624863637111517	29.034504832918874	21.522272828324144
40-41	22.107795203738643	27.43824308391622	28.857595448637962	21.59636626370718
42-43	21.951608188439863	27.576948593793492	28.67389332108683	21.79754989667982
44-45	22.03430720321296	27.639330133697467	28.60590542553888	21.720457237550697
46-47	22.059291627603017	27.62271289272864	28.426227489628843	21.891767990039497
48-49	22.040629413225165	27.743274733819405	28.372023355715626	21.8440724972398
50-51	22.02713970837794	27.847894808368967	28.24628934266917	21.878676140583927
52-53	22.15297246052689	27.766602889190782	28.173298609664577	21.90712604061775
54-55	22.14057224161832	27.6667675265992	28.199822345799834	21.992837885982645
56-57	22.093793266406177	27.78012216056248	28.176433776864805	21.94965079616653
58-59	22.1255032188553	27.777753971952578	28.194521973049554	21.902220836142565
60-61	22.094891915684425	27.77451634183504	28.261286096151967	21.869305646328574
62-63	22.093291375670574	27.758833767724273	28.280134878840478	21.867739977764668
64-65	21.983153946885878	27.76970471169643	28.286878628438235	21.960262712979457
66-67	22.047546654855815	27.916335211969585	28.092529455485682	21.94358867768892
68-69	21.965426268722887	27.778474260228318	28.229926969137193	22.0261725019116
70-71	22.141443135371546	27.55417532528139	28.15584370455132	22.148537834795746
72-73	22.227131189425474	27.720795072403508	28.120494675160266	21.931579063010744
74-75	22.06610152267146	27.849648668613774	28.00314395905516	22.081105849659604
76-77	22.199813301679132	27.613857164259947	28.020536815295433	22.165792718765488
78-79	22.134260096873433	27.678825247190797	27.942956588929718	22.24395806700605
80-81	22.257502550521593	27.723824146040478	27.920800618623325	22.097872684814604
82-83	22.261882732193527	27.66139231491272	27.983541360495007	22.093183592398745
84-85	22.383253520295842	27.669012298972845	27.84379297817289	22.103941202558428
86-87	22.215064627928886	27.729623346307697	27.91333084778518	22.14198117797824
88-89	22.32883791037167	27.657647774506266	27.86059828828603	22.15291602683603
90-91	22.347206509584446	27.72433664443115	27.85045833394116	22.077998512043244
92-93	22.478716028487387	27.724067965966036	27.782184678599286	22.015031326947295
94-95	22.292071238072158	27.810452124735484	27.83683391628652	22.060642720905836
96-97	22.37661364940416	27.853544076447566	27.933034454073557	21.83680782007472
98-99	23.435395486213192	29.748566509187786	28.122443247209933	18.693594757389086
100	29.70627717594082	40.92685654954717	29.366866274512006	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	877.0
1	905.0
2	1231.0
3	1665.0
4	2212.5
5	3175.5
6	3947.5
7	4654.0
8	5354.0
9	5656.5
10	5504.0
11	5459.5
12	6084.5
13	7611.0
14	10627.0
15	14327.5
16	17341.5
17	18931.0
18	18989.5
19	18109.0
20	17109.5
21	16691.0
22	18139.0
23	21758.0
24	27559.0
25	36030.5
26	47161.5
27	60134.0
28	73147.0
29	86659.0
30	99816.0
31	111778.5
32	124141.5
33	136940.5
34	149346.0
35	161414.5
36	175133.5
37	187556.0
38	195923.0
39	202049.5
40	207906.5
41	213804.5
42	218010.5
43	221407.5
44	225110.0
45	228570.5
46	229488.5
47	228323.5
48	226818.5
49	223946.5
50	218446.0
51	209967.0
52	199522.0
53	187496.5
54	174823.5
55	161476.5
56	146500.0
57	130722.5
58	114803.0
59	97018.0
60	79802.0
61	64828.5
62	50894.5
63	38739.0
64	28864.0
65	21541.0
66	15711.0
67	11440.5
68	8367.5
69	6020.5
70	4300.5
71	2960.5
72	1961.5
73	1249.0
74	824.5
75	545.0
76	332.5
77	209.5
78	126.5
79	80.5
80	56.5
81	40.5
82	29.0
83	18.0
84	9.5
85	7.0
86	7.0
87	5.5
88	4.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010267637441071564
3	7.898182646978127E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	4.701331464083943E-5
22-23	0.0
24-25	9.403494056286494E-5
26-27	1.6928102399219952E-4
28-29	0.0017309522579848874
30-31	0.0021658492469106747
32-33	0.0020480353960919333
34-35	4.921907780674867E-4
36-37	9.505014940932985E-6
38-39	6.696173834968587E-5
40-41	1.8306234004928038E-4
42-43	0.002067137320514494
44-45	1.9573989730114807E-4
46-47	5.039914143580237E-4
48-49	9.77935187644807E-4
50-51	0.0012592598413171414
52-53	0.0
54-55	0.0027357465555209004
56-57	0.052702717793438436
58-59	0.06974535634101532
60-61	0.04555254380937026
62-63	0.0071099388417344665
64-65	0.0
66-67	0.0
68-69	1.6510092289214552E-4
70-71	0.001514700952368224
72-73	0.0071995505858000524
74-75	0.011254826589094939
76-77	0.012594787878075539
78-79	0.0038609768388048003
80-81	0.00816336560323964
82-83	0.04614533327351309
84-85	0.03904026342006028
86-87	0.008688858591583817
88-89	0.0022089026221411463
90-91	0.002188123819639241
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	20.0
20-21	112.0
22-23	301.0
24-25	452.0
26-27	1177.0
28-29	3241.0
30-31	11365.0
32-33	14218.0
34-35	19144.0
36-37	31609.0
38-39	37576.0
40-41	36793.0
42-43	41246.0
44-45	48180.0
46-47	49583.0
48-49	47958.0
50-51	44020.0
52-53	40320.0
54-55	43653.0
56-57	46839.0
58-59	48859.0
60-61	48706.0
62-63	46804.0
64-65	48185.0
66-67	51501.0
68-69	53144.0
70-71	52641.0
72-73	48715.0
74-75	49459.0
76-77	51810.0
78-79	54097.0
80-81	54003.0
82-83	50545.0
84-85	49126.0
86-87	50107.0
88-89	52660.0
90-91	53285.0
92-93	52445.0
94-95	58268.0
96-97	162950.0
98-99	1170008.0
100-101	2492554.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.30518065802106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.09371797068174	79.5295350424119
2	6.403520763400506	11.181210741921403
3	1.4431230675085687	3.779763603617793
4	0.5012643933721126	1.7505191368314248
5	0.22312809159686403	0.9740119173371843
6	0.12095198856572878	0.633584112760671
7	0.07036766578418402	0.43004232416400034
8	0.037703048715754126	0.26333371835896663
9	0.023260167027836953	0.18276597760509447
>10	0.08114802894104849	1.0872415958309525
>50	0.0010680638560094245	0.06084635080670658
>100	7.46750549678513E-4	0.12714547835392978
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.76103935570387E-5	0.0	0.0	0.0
3	0.0	3.76103935570387E-5	0.0	0.0	0.0
4	0.0	7.52207871140774E-5	0.0	0.0	5.641559033555805E-5
5	0.0	7.52207871140774E-5	0.0	0.0	5.641559033555805E-5
6	0.0	7.52207871140774E-5	0.0	0.0	7.52207871140774E-5
7	0.0	7.52207871140774E-5	0.0	3.76103935570387E-5	7.52207871140774E-5
8	0.0	7.52207871140774E-5	0.0	3.76103935570387E-5	7.52207871140774E-5
9	3.76103935570387E-5	9.402598389259676E-5	0.0	3.76103935570387E-5	1.128311806711161E-4
10-11	7.52207871140774E-5	9.402598389259676E-5	0.0	3.76103935570387E-5	1.128311806711161E-4
12-13	7.52207871140774E-5	9.402598389259676E-5	0.0	3.76103935570387E-5	1.128311806711161E-4
14-15	9.402598389259676E-5	1.2223377906037577E-4	0.0	3.76103935570387E-5	1.2223377906037577E-4
16-17	9.402598389259676E-5	1.410389758388951E-4	0.0	3.76103935570387E-5	1.3163637744963546E-4
18-19	1.128311806711161E-4	1.8805196778519352E-4	0.0	3.76103935570387E-5	1.3163637744963546E-4
20-21	1.504415742281548E-4	2.256623613422322E-4	0.0	3.76103935570387E-5	1.410389758388951E-4
22-23	1.8805196778519352E-4	2.5387015651001123E-4	0.0	5.641559033555805E-5	1.5984417261741447E-4
24-25	1.974545661744532E-4	3.102857468455693E-4	0.0	1.0342858228185644E-4	1.8805196778519352E-4
26-27	2.0685716456371285E-4	3.384935420133483E-4	0.0	1.7864936939593383E-4	1.8805196778519352E-4
28-29	2.256623613422322E-4	3.5729873879186767E-4	0.0	4.04311730738166E-4	1.974545661744532E-4
30-31	2.3506495973149187E-4	4.513247226844644E-4	0.0	0.00144800015194599	2.0685716456371285E-4
32-33	2.4446755812075155E-4	4.513247226844644E-4	0.0	0.004372208251005749	2.256623613422322E-4
34-35	2.5387015651001123E-4	5.359481081878014E-4	0.0	0.007992208630870724	2.820779516777903E-4
36-37	3.384935420133483E-4	5.829611001340999E-4	0.0	0.014009871599996917	2.9148055006704995E-4
38-39	3.7610393557038703E-4	5.829611001340999E-4	0.0	0.03146109421046288	3.008831484563096E-4
40-41	4.04311730738166E-4	6.111688953018789E-4	0.0	0.05057657673582779	3.008831484563096E-4
42-43	4.137143291274257E-4	6.487792888589176E-4	0.0	0.06222639614012053	3.008831484563096E-4
44-45	4.137143291274257E-4	7.145974775837353E-4	0.0	0.07616104695300337	3.008831484563096E-4
46-47	4.3251952590594506E-4	7.522078711407741E-4	0.0	0.09103595760481217	3.1028574684556926E-4
48-49	4.607273210737241E-4	7.522078711407741E-4	0.0	0.10460390708051388	3.384935420133483E-4
50-51	4.983377146307627E-4	8.08623461476332E-4	0.0	0.11902749300963822	3.384935420133483E-4
52-53	5.077403130200225E-4	8.274286582548514E-4	0.0	0.16034251033204522	3.5729873879186767E-4
54-55	5.171429114092822E-4	8.274286582548514E-4	0.0	0.2107874506904234	3.7610393557038703E-4
56-57	5.359481081878014E-4	8.650390518118901E-4	0.0	0.28223779585040765	3.7610393557038703E-4
58-59	5.453507065770612E-4	8.650390518118901E-4	0.0	0.3270223719784515	3.7610393557038703E-4
60-61	5.453507065770612E-4	8.932468469796692E-4	0.0	0.3461190493070379	3.7610393557038703E-4
62-63	5.641559033555805E-4	9.120520437581885E-4	0.0	0.3619248171993834	3.7610393557038703E-4
64-65	6.017662969126192E-4	9.684676340937465E-4	0.0	0.3715718831467638	3.8550653395964666E-4
66-67	6.205714936911385E-4	9.778702324830062E-4	0.0	0.3797991567373661	3.9490913234890634E-4
68-69	6.205714936911385E-4	9.966754292615254E-4	0.0	0.38794180694246494	3.9490913234890634E-4
70-71	6.205714936911385E-4	0.001015480626040045	0.0	0.3947210803811212	4.3251952590594506E-4
72-73	6.299740920803982E-4	0.001015480626040045	0.0	0.3993001457966906	4.7012991946298373E-4
74-75	6.581818872481773E-4	0.0010248832244293044	0.0	0.4028919383813878	4.7012991946298373E-4
76-77	6.581818872481773E-4	0.0011001040115433819	0.0	0.40673760112259505	4.7012991946298373E-4
78-79	6.581818872481773E-4	0.0011471170034896803	0.0	0.4094079390651448	4.7012991946298373E-4
80-81	6.581818872481773E-4	0.00115651960187894	0.0	0.4103293937072922	4.795325178522434E-4
82-83	6.581818872481773E-4	0.0011941299954359788	0.0	0.4109687703977619	5.077403130200225E-4
84-85	6.581818872481773E-4	0.001212935192214498	0.0	0.4112602509478289	5.077403130200225E-4
86-87	6.581818872481773E-4	0.001241142987382277	0.0	0.4114012899236678	5.077403130200225E-4
88	6.581818872481773E-4	0.0012787533809393158	0.0	0.41143890031722485	5.265455097985418E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5685	0.0	45.5357	1
GTATCAA	10485	0.0	35.831985	1
TCAACGC	12135	0.0	30.471056	4
ATCAACG	12325	0.0	30.00132	3
CAACGCA	12410	0.0	29.82969	5
TATCAAC	12945	0.0	28.957735	2
AACGCAG	12860	0.0	28.85123	6
ACGCAGA	14810	0.0	24.99571	7
CGCAGAG	15175	0.0	24.449875	8
GCAGAGT	18570	0.0	20.09304	9
CGTCGAA	270	5.6201464E-4	19.919256	94
TATACCG	470	0.0	19.668419	5
GTACATG	14185	0.0	19.405006	1
TACATGG	14060	0.0	19.248741	2
ACATGGG	14085	0.0	18.6154	3
GAGTACT	10790	0.0	18.322418	12-13
AGAGTAC	16560	0.0	18.040722	10-11
CAGAGTA	18110	0.0	17.540743	10-11
CATGGGG	10220	0.0	16.89801	4
GTACTTT	12325	0.0	16.074646	14-15
>>END_MODULE