FastQCFastQC Report
Fri 27 May 2016
SRR2138679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5620600
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65550.047.836741
GTATCAA121200.035.9198531
TCAACGC141650.030.8220544
ATCAACG143050.030.5500623
CAACGCA143500.030.4838315
AACGCAG148300.029.5543866
TATCAAC150100.029.5127472
ACGCAGA172600.025.3934847
CGCAGAG175650.024.9525498
GCAGAGT210550.020.8165079
GTACATG157700.019.5610681
GAGTACT118450.019.16379412-13
TACATGG159850.018.9528882
TATACCG5200.018.7666725
ACATGGG160250.018.5866833
CAGAGTA205050.018.43659410-11
AGAGTAC189450.017.96149810-11
GTACTTT133900.016.87344614-15
CATGGGG109850.016.0677854
AGTACTT133800.015.58583912-13
Produced by FastQC (version 0.11.5)