##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138679_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5620600
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.29587926555884	30.0	18.0	33.0	18.0	33.0
2	31.149547913034194	32.0	31.0	33.0	27.0	34.0
3	31.45475572002989	33.0	31.0	33.0	29.0	34.0
4	32.72463260150162	33.0	33.0	33.0	32.0	34.0
5	32.67672276981105	33.0	33.0	33.0	32.0	34.0
6	36.972698288438956	38.0	37.0	38.0	35.0	38.0
7	37.0399293669715	38.0	38.0	38.0	35.0	38.0
8	37.365209764082124	38.0	38.0	38.0	37.0	38.0
9	37.45314717289969	38.0	38.0	38.0	37.0	38.0
10-11	37.468565900437675	38.0	38.0	38.0	37.0	38.0
12-13	37.496010657225206	38.0	38.0	38.0	37.0	38.0
14-15	37.50498158559584	38.0	38.0	38.0	37.0	38.0
16-17	37.53509482973348	38.0	38.0	38.0	37.5	38.0
18-19	37.53755399779383	38.0	38.0	38.0	38.0	38.0
20-21	37.54059778072588	38.0	38.0	38.0	38.0	38.0
22-23	37.54984057789514	38.0	38.0	38.0	38.0	38.0
24-25	37.559817633670676	38.0	38.0	38.0	38.0	38.0
26-27	37.53057414563152	38.0	38.0	38.0	38.0	38.0
28-29	37.507269896496624	38.0	38.0	38.0	38.0	38.0
30-31	37.50842341830088	38.0	38.0	38.0	38.0	38.0
32-33	37.49697925911636	38.0	38.0	38.0	38.0	38.0
34-35	37.399584838747884	38.0	38.0	38.0	37.0	38.0
36-37	37.44249312424158	38.0	38.0	38.0	37.0	38.0
38-39	37.448255024234925	38.0	38.0	38.0	37.0	38.0
40-41	37.44368712091725	38.0	38.0	38.0	37.0	38.0
42-43	37.410734463354586	38.0	38.0	38.0	37.0	38.0
44-45	37.383952219081934	38.0	38.0	38.0	37.0	38.0
46-47	37.35908722125512	38.0	38.0	38.0	37.0	38.0
48-49	37.30628015767667	38.0	38.0	38.0	37.0	38.0
50-51	37.27806186934207	38.0	38.0	38.0	37.0	38.0
52-53	37.25694328269087	38.0	38.0	38.0	37.0	38.0
54-55	37.229331009308666	38.0	38.0	38.0	37.0	38.0
56-57	37.1844217692684	38.0	38.0	38.0	37.0	38.0
58-59	37.17488507735838	38.0	38.0	38.0	37.0	38.0
60-61	37.17570285799475	38.0	38.0	38.0	36.5	38.0
62-63	37.180316031862745	38.0	38.0	38.0	36.0	38.0
64-65	37.184638053089884	38.0	38.0	38.0	36.0	38.0
66-67	37.1819627680088	38.0	38.0	38.0	36.0	38.0
68-69	37.17126774441469	38.0	38.0	38.0	36.0	38.0
70-71	37.16547096966923	38.0	38.0	38.0	36.0	38.0
72-73	37.15554109933894	38.0	38.0	38.0	36.0	38.0
74-75	37.15322938174813	38.0	38.0	38.0	36.0	38.0
76-77	37.13903069886567	38.0	38.0	38.0	36.0	38.0
78-79	37.124006286029584	38.0	38.0	38.0	36.0	38.0
80-81	37.11396731184203	38.0	38.0	38.0	36.0	38.0
82-83	37.06530719605368	38.0	38.0	38.0	36.0	38.0
84-85	37.0550220569171	38.0	38.0	38.0	36.0	38.0
86-87	37.04080496034986	38.0	38.0	38.0	36.0	38.0
88-89	37.02489396184731	38.0	38.0	38.0	36.0	38.0
90-91	37.013726770427695	38.0	38.0	38.0	36.0	38.0
92-93	36.99427208535363	38.0	38.0	38.0	36.0	38.0
94-95	36.977625580533356	38.0	38.0	38.0	36.0	38.0
96-97	36.96257786353871	38.0	38.0	38.0	35.0	38.0
98-99	36.977095418628366	38.0	38.0	38.0	35.0	38.0
100	35.58390252032316	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	9.0
20	11.0
21	58.0
22	254.0
23	728.0
24	1734.0
25	3813.0
26	7019.0
27	12000.0
28	19199.0
29	27738.0
30	37273.0
31	47568.0
32	61620.0
33	83101.0
34	124640.0
35	231163.0
36	774291.0
37	4188380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.456783973241286	22.545849197594563	13.601466035654555	24.39590079350959
2	14.186617927068893	21.13740200079323	41.96354458867157	22.71243548346631
3	17.545302037005968	26.972061290054555	31.255323097211253	24.227313575728225
4	12.598761698039354	18.272141764224457	39.69549514286731	29.433601394868873
5	13.138348219051347	37.81964558943885	35.893392164537595	13.148614026972211
6	29.289381916521368	39.07445824289222	18.948386293278297	12.687773547308117
7	25.758335409031062	33.34642209016831	24.125520407073978	16.76972209372665
8	23.692684055083088	37.937835818240046	21.464434402021137	16.905045724655732
9	25.884620858983027	17.04145464896986	21.652599366615664	35.421325125431444
10-11	23.964381026936625	27.08365654912287	29.37812689036758	19.57383553357293
12-13	24.842214354339394	24.861206988577734	29.431920079706792	20.86465857737608
14-15	22.08535565597979	26.08505319716756	26.95267409173398	24.87691705511867
16-17	21.570606340960037	29.107862149948403	27.634585275593356	21.6869462334982
18-19	21.60692808596947	28.43657260790663	29.231309824573888	20.725189481550014
20-21	22.46098149323072	27.61882403428284	29.02702552673783	20.89316894574861
22-23	22.25488523237937	27.465114760503646	28.830971057532466	21.44902894958452
24-25	21.9321473102739	27.651561353850973	28.934790083882817	21.48150125199231
26-27	21.869172316452797	27.826906159408004	28.85217362058155	21.451747903557646
28-29	21.88983307912827	27.884159392983943	28.83757969720403	21.388427830683764
30-31	22.1488735602609	27.756834381235706	28.74389186406584	21.350400194437558
32-33	21.89363977275223	27.87806974019057	28.723562288611237	21.50472819844596
34-35	22.03955897682925	27.917672118549657	28.685955053313677	21.356813851307415
36-37	22.05195479157581	27.806160175192794	28.680390126339855	21.46149490689154
38-39	21.882354195734017	27.97445799512486	28.789185739472025	21.3540020696691
40-41	22.145999194516307	27.78364831723397	28.683960069255587	21.386392418994134
42-43	22.037040427713492	27.927951516039258	28.39216529954592	21.642842756701334
44-45	22.095868882318147	28.025146190414514	28.31454730694876	21.56443762031858
46-47	22.114743252133746	28.05197396592467	28.098089120164737	21.735193661776847
48-49	22.117670651850315	28.086245281401546	28.08896797854665	21.70711608820149
50-51	22.10461119326305	28.19959503548765	27.978830961541245	21.71696280970805
52-53	22.2103670130379	28.150571144662596	27.90239425285415	21.736667589445357
54-55	22.218679596321465	28.08493145836411	27.91335615489733	21.7830327904171
56-57	22.224472038451278	28.196278109382998	27.860947638126525	21.71830221403919
58-59	22.188547817844714	28.21921727011994	27.871561565459764	21.720673346575587
60-61	22.271803946573907	28.22102110698157	27.935266691107184	21.571908255337334
62-63	22.27251987379741	28.265298584721847	27.884724946922958	21.577456594557784
64-65	22.23111472576464	28.289152037274906	27.797322515115074	21.682410721845372
66-67	22.316556362240796	28.484617858933287	27.581540232990797	21.617285545835117
68-69	22.211937379938025	28.21259602907571	27.830294497367863	21.745172093618404
70-71	22.335108579041947	27.957566084777696	27.8058077005362	21.901517635644158
72-73	22.304224930486395	28.220708120309173	27.764181796201232	21.7108851530032
74-75	22.1481117635326	28.285330832871686	27.736848608653126	21.82970879494259
76-77	22.23251139777459	27.900833754286513	27.927073465852597	21.9395813820863
78-79	22.17179694881032	27.919543978759197	27.910971826228003	21.997687246202478
80-81	22.16110942529524	28.005611637596445	27.881721459807597	21.951557477300724
82-83	22.242757863030455	27.883078023110496	27.993943968450658	21.880220145408394
84-85	22.35837612462184	27.908366135676125	27.841542378088242	21.891715361613794
86-87	22.14732976233203	27.94780288614555	27.9453198890326	21.959547462489816
88-89	22.267144178551668	27.86369290596225	27.90836118774936	21.96080172773672
90-91	22.310029706806038	27.893917898018454	27.87354797890079	21.92250441627472
92-93	22.360007054733128	27.910475062360472	27.817682866354907	21.91183501655149
94-95	22.180474407937307	27.897239833121883	27.940042755560974	21.982243003379832
96-97	22.27504955823491	28.08920422086268	27.908019434413067	21.727726786489338
98-99	23.32963574098229	29.99584980049519	28.155689577562754	18.518824880959766
100	29.64334595518931	40.94007116524055	29.416582879570136	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1003.0
1	989.5
2	1164.0
3	1482.5
4	1913.0
5	2674.5
6	3319.5
7	3910.5
8	4511.5
9	4805.5
10	4932.5
11	5275.5
12	6092.5
13	7726.5
14	10905.5
15	14756.0
16	17823.5
17	19636.5
18	19941.0
19	19034.5
20	18112.0
21	17892.5
22	19233.0
23	23156.5
24	29641.5
25	38331.0
26	49680.0
27	62840.0
28	76055.5
29	90973.0
30	105838.5
31	118811.5
32	132804.0
33	147905.0
34	161806.0
35	175396.0
36	190274.0
37	202453.5
38	211371.5
39	217582.5
40	222051.0
41	226229.0
42	227855.0
43	228816.5
44	232167.0
45	235025.0
46	234046.0
47	233149.5
48	231810.5
49	228002.0
50	223073.0
51	216228.5
52	206720.5
53	194844.0
54	181493.0
55	166504.0
56	150433.0
57	133793.0
58	117119.5
59	99345.0
60	81536.5
61	65304.5
62	51005.0
63	38683.5
64	28309.0
65	20853.0
66	15355.0
67	11590.0
68	8711.5
69	6185.5
70	4407.0
71	3090.5
72	2098.5
73	1363.0
74	885.0
75	598.5
76	388.5
77	225.0
78	127.0
79	80.5
80	51.5
81	37.0
82	29.0
83	19.5
84	11.5
85	5.0
86	1.5
87	1.5
88	1.5
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009411806568693734
3	8.362096573319575E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.7791887931032946E-5
22-23	0.0
24-25	8.896721958310851E-5
26-27	1.334645913100671E-4
28-29	0.0016288084411795883
30-31	0.0024856456192767786
32-33	0.0022585714348204405
34-35	4.564621072009314E-4
36-37	0.0
38-39	1.807413086700973E-5
40-41	1.2733465822013435E-4
42-43	0.002187955642906964
44-45	1.6605181370093515E-4
46-47	4.001904534288132E-4
48-49	0.0010609017702601256
50-51	0.0011076897908113628
52-53	0.0
54-55	0.0029045482725343417
56-57	0.05345577519314416
58-59	0.06947108868029504
60-61	0.04578276424158638
62-63	0.00765554741599856
64-65	0.0
66-67	0.0
68-69	1.6404683865337233E-4
70-71	0.0015022279594667817
72-73	0.007368187161164127
74-75	0.01190051046425836
76-77	0.01353986540006992
78-79	0.0040591920890664475
80-81	0.007664748696146543
82-83	0.046567411932984894
84-85	0.03915874775504172
86-87	0.008859000264641474
88-89	0.0020318917974240405
90-91	0.0027613591802714637
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	35.0
20-21	147.0
22-23	292.0
24-25	445.0
26-27	1375.0
28-29	3459.0
30-31	10982.0
32-33	13352.0
34-35	18369.0
36-37	30252.0
38-39	36152.0
40-41	34850.0
42-43	39442.0
44-45	46707.0
46-47	47541.0
48-49	45098.0
50-51	42735.0
52-53	40089.0
54-55	43849.0
56-57	46459.0
58-59	46326.0
60-61	45846.0
62-63	43200.0
64-65	45669.0
66-67	48933.0
68-69	50165.0
70-71	49849.0
72-73	46474.0
74-75	47528.0
76-77	50344.0
78-79	52043.0
80-81	52292.0
82-83	49246.0
84-85	48146.0
86-87	49914.0
88-89	52166.0
90-91	52692.0
92-93	51720.0
94-95	58325.0
96-97	170279.0
98-99	1265744.0
100-101	2742069.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.17720262530919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.31994622519153	77.8352030695536
2	6.816912217312156	11.74924850860511
3	1.6299114372939592	4.2138362457897145
4	0.6047744670757155	2.0847108716718927
5	0.25747947342016575	1.1094430376393767
6	0.13151504089342542	0.6800158996409128
7	0.07307833262071628	0.4408380394442646
8	0.04488876135924711	0.30947103066039905
9	0.02719436272706751	0.2109180696296987
>10	0.09217545704268282	1.1806860111788202
>50	0.0014488514867928995	0.087458350061645
>100	6.75373576541456E-4	0.09817086612455121
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.779169483685016E-5	0.0	0.0	0.0
3	0.0	1.779169483685016E-5	0.0	0.0	0.0
4	0.0	1.779169483685016E-5	0.0	3.558338967370032E-5	0.0
5	0.0	1.779169483685016E-5	0.0	3.558338967370032E-5	0.0
6	1.2454186385795111E-4	1.779169483685016E-5	0.0	3.558338967370032E-5	0.0
7	1.2454186385795111E-4	1.779169483685016E-5	0.0	3.558338967370032E-5	0.0
8	1.2454186385795111E-4	1.779169483685016E-5	0.0	3.558338967370032E-5	0.0
9	1.779169483685016E-4	3.558338967370032E-5	0.0	5.337508451055048E-5	0.0
10-11	1.8681279578692666E-4	3.558338967370032E-5	0.0	9.785432160267587E-5	0.0
12-13	1.9570864320535175E-4	3.558338967370032E-5	0.0	1.2454186385795111E-4	0.0
14-15	2.135003380422019E-4	8.006262676582572E-5	0.0	1.334377112763762E-4	8.89584741842508E-6
16-17	3.736255915738533E-4	8.89584741842508E-5	0.0	1.4233355869480127E-4	1.779169483685016E-5
18-19	4.0031313382912857E-4	1.1564601643952603E-4	0.0	1.4233355869480127E-4	1.779169483685016E-5
20-21	4.181048286659787E-4	1.4233355869480127E-4	0.0	1.4233355869480127E-4	1.779169483685016E-5
22-23	4.270006760844038E-4	1.6902110095007652E-4	0.0	1.6012525353165143E-4	3.558338967370032E-5
24-25	4.270006760844038E-4	1.779169483685016E-4	0.0	1.8681279578692666E-4	5.337508451055048E-5
26-27	4.4479237092125395E-4	1.779169483685016E-4	0.0	2.2239618546062698E-4	5.337508451055048E-5
28-29	4.4479237092125395E-4	1.779169483685016E-4	0.0	4.892716080133793E-4	5.337508451055048E-5
30-31	4.5368821833967907E-4	2.135003380422019E-4	0.0	0.0013076895705084867	5.337508451055048E-5
32-33	4.714799131765292E-4	2.135003380422019E-4	0.0	0.0030423798171013774	5.337508451055048E-5
34-35	4.981674554318045E-4	2.668754225527524E-4	0.0	0.0053641959933103224	7.116677934740064E-5
36-37	5.159591502686546E-4	3.3804220190015305E-4	0.0	0.008575596911361777	7.116677934740064E-5
38-39	5.515425399423549E-4	4.0031313382912857E-4	0.0	0.013797459345977297	7.116677934740064E-5
40-41	6.049176244529054E-4	4.270006760844038E-4	0.0	0.01917944703412447	7.116677934740064E-5
42-43	6.405010141266057E-4	4.270006760844038E-4	0.0	0.024374621926484714	7.116677934740064E-5
44-45	6.582927089634559E-4	4.4479237092125395E-4	0.0	0.0308952780841903	7.116677934740064E-5
46-47	6.582927089634559E-4	4.714799131765292E-4	0.0	0.03778955983346974	7.116677934740064E-5
48-49	6.582927089634559E-4	4.8037576059495427E-4	0.0	0.044630466498238625	7.116677934740064E-5
50-51	7.116677934740064E-4	5.337508451055047E-4	0.0	0.051222289435291606	7.116677934740064E-5
52-53	7.294594883108565E-4	5.337508451055047E-4	0.0	0.05756502864462869	8.006262676582572E-5
54-55	7.294594883108565E-4	5.693342347792051E-4	0.0	0.06387218446429208	8.89584741842508E-5
56-57	7.294594883108565E-4	6.049176244529054E-4	0.0	0.07070419528164253	9.785432160267587E-5
58-59	7.472511831477066E-4	6.405010141266057E-4	0.0	0.07731380991353237	1.0675016902110095E-4
60-61	8.095221150766821E-4	6.405010141266057E-4	0.0	0.0841725082731381	1.0675016902110095E-4
62-63	9.429598263530584E-4	6.582927089634559E-4	0.0	0.09117354019143864	1.2454186385795111E-4
64-65	9.607515211899085E-4	6.849802512187311E-4	0.0	0.09763192541721524	1.2454186385795111E-4
66-67	0.001005230758282034	6.938760986371561E-4	0.0	0.10433939437070774	1.2454186385795111E-4
68-69	0.001014126605700459	7.205636408924314E-4	0.0	0.11087784222325019	1.2454186385795111E-4
70-71	0.0010586058427925844	7.472511831477066E-4	0.0	0.11669572643490018	1.5122940611322634E-4
72-73	0.0011920435540689607	7.472511831477066E-4	0.0	0.12116144183894959	2.3129203287905207E-4
74-75	0.0011920435540689607	7.739387254029819E-4	0.0	0.12448848877344056	2.3129203287905207E-4
76-77	0.0011920435540689607	8.273138099135324E-4	0.0	0.12811799452015799	2.4018788029747716E-4
78-79	0.0011920435540689607	8.362096573319574E-4	0.0	0.13069779027150125	2.4908372771590223E-4
80-81	0.0012009394014873857	8.451055047503825E-4	0.0	0.13151620823399637	2.4908372771590223E-4
82-83	0.0012187310963242357	8.628971995872326E-4	0.0	0.13213891755328613	2.4908372771590223E-4
84-85	0.001227626943742661	8.895847418425079E-4	0.0	0.13250364729744155	2.4908372771590223E-4
86-87	0.0012365227911610862	9.251681315162083E-4	0.0	0.1326281891612995	2.4908372771590223E-4
88	0.0012632103334163612	0.001014126605700459	0.0	0.13265487670355477	2.4908372771590223E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6555	0.0	47.83674	1
GTATCAA	12120	0.0	35.919853	1
TCAACGC	14165	0.0	30.822054	4
ATCAACG	14305	0.0	30.550062	3
CAACGCA	14350	0.0	30.483831	5
AACGCAG	14830	0.0	29.554386	6
TATCAAC	15010	0.0	29.512747	2
ACGCAGA	17260	0.0	25.393484	7
CGCAGAG	17565	0.0	24.952549	8
GCAGAGT	21055	0.0	20.816507	9
GTACATG	15770	0.0	19.561068	1
GAGTACT	11845	0.0	19.163794	12-13
TACATGG	15985	0.0	18.952888	2
TATACCG	520	0.0	18.766672	5
ACATGGG	16025	0.0	18.586683	3
CAGAGTA	20505	0.0	18.436594	10-11
AGAGTAC	18945	0.0	17.961498	10-11
GTACTTT	13390	0.0	16.873446	14-15
CATGGGG	10985	0.0	16.067785	4
AGTACTT	13380	0.0	15.585839	12-13
>>END_MODULE