FastQCFastQC Report
Fri 27 May 2016
SRR2138678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4604481
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49450.046.7086261
GTATCAA93900.037.593841
TCAACGC107300.032.3044934
ATCAACG108150.032.1290173
CAACGCA109100.031.771515
AACGCAG112300.031.0917556
TATCAAC117350.029.901572
ACGCAGA130800.026.6296637
CGCAGAG134400.025.8849548
GCAGAGT159050.021.899789
GAGTACT90400.019.35867312-13
GTACATG122950.018.7173351
AGAGTAC141950.018.47037310-11
CAGAGTA156900.018.43262110-11
TACATGG123650.018.1332842
ACATGGG122850.017.6993063
TATACCG3151.551598E-917.4241755
GTACTTT102350.016.87191414-15
AGTACTT100400.015.9166512-13
CATGGGG86750.015.4279854
Produced by FastQC (version 0.11.5)