##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138678_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4604481
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.433193447860898	30.0	18.0	33.0	18.0	33.0
2	31.17813820927918	32.0	31.0	33.0	27.0	34.0
3	31.431288998694967	33.0	31.0	33.0	29.0	34.0
4	32.73137015876491	33.0	33.0	33.0	32.0	34.0
5	32.654346277028836	33.0	33.0	33.0	32.0	34.0
6	36.95131677164049	38.0	37.0	38.0	35.0	38.0
7	37.0211509179862	38.0	38.0	38.0	35.0	38.0
8	37.35884565491746	38.0	38.0	38.0	37.0	38.0
9	37.447195460248395	38.0	38.0	38.0	37.0	38.0
10-11	37.464037857904074	38.0	38.0	38.0	37.0	38.0
12-13	37.49414092489469	38.0	38.0	38.0	37.0	38.0
14-15	37.50181366803338	38.0	38.0	38.0	37.0	38.0
16-17	37.53195463288914	38.0	38.0	38.0	37.5	38.0
18-19	37.53579078727874	38.0	38.0	38.0	38.0	38.0
20-21	37.54153335112689	38.0	38.0	38.0	38.0	38.0
22-23	37.55187259322086	38.0	38.0	38.0	38.0	38.0
24-25	37.562860857978094	38.0	38.0	38.0	38.0	38.0
26-27	37.53371396968243	38.0	38.0	38.0	38.0	38.0
28-29	37.50865778995212	38.0	38.0	38.0	38.0	38.0
30-31	37.51192683294244	38.0	38.0	38.0	38.0	38.0
32-33	37.50147736829286	38.0	38.0	38.0	38.0	38.0
34-35	37.401940457958176	38.0	38.0	38.0	37.0	38.0
36-37	37.44590945599447	38.0	38.0	38.0	37.0	38.0
38-39	37.45211270072322	38.0	38.0	38.0	37.0	38.0
40-41	37.448189052777224	38.0	38.0	38.0	37.0	38.0
42-43	37.416621709274025	38.0	38.0	38.0	37.0	38.0
44-45	37.38853972952276	38.0	38.0	38.0	37.0	38.0
46-47	37.36468241077772	38.0	38.0	38.0	37.0	38.0
48-49	37.31222160827643	38.0	38.0	38.0	37.0	38.0
50-51	37.27982671152846	38.0	38.0	38.0	37.0	38.0
52-53	37.25493711597865	38.0	38.0	38.0	37.0	38.0
54-55	37.225662731843315	38.0	38.0	38.0	37.0	38.0
56-57	37.17802874296224	38.0	38.0	38.0	37.0	38.0
58-59	37.16818408607766	38.0	38.0	38.0	36.0	38.0
60-61	37.1665967904126	38.0	38.0	38.0	36.0	38.0
62-63	37.170335729399355	38.0	38.0	38.0	36.0	38.0
64-65	37.174110311414026	38.0	38.0	38.0	36.0	38.0
66-67	37.16872924960963	38.0	38.0	38.0	36.0	38.0
68-69	37.157115259260884	38.0	38.0	38.0	36.0	38.0
70-71	37.14993957243055	38.0	38.0	38.0	36.0	38.0
72-73	37.13890565458152	38.0	38.0	38.0	36.0	38.0
74-75	37.134256539587234	38.0	38.0	38.0	36.0	38.0
76-77	37.11826921293263	38.0	38.0	38.0	36.0	38.0
78-79	37.104405114425546	38.0	38.0	38.0	36.0	38.0
80-81	37.094287668524814	38.0	38.0	38.0	36.0	38.0
82-83	37.04380892027138	38.0	38.0	38.0	36.0	38.0
84-85	37.03169103235071	38.0	38.0	38.0	36.0	38.0
86-87	37.0177813687784	38.0	38.0	38.0	36.0	38.0
88-89	37.00176189027586	38.0	38.0	38.0	36.0	38.0
90-91	36.990595643633974	38.0	38.0	38.0	36.0	38.0
92-93	36.97087335567687	38.0	38.0	38.0	36.0	38.0
94-95	36.953728944170194	38.0	38.0	38.0	35.0	38.0
96-97	36.94040690133741	38.0	38.0	38.0	35.0	38.0
98-99	36.95350793737596	38.0	38.0	38.0	35.0	38.0
100	35.525829694039615	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	3.0
20	6.0
21	46.0
22	195.0
23	523.0
24	1401.0
25	2985.0
26	5541.0
27	9544.0
28	15341.0
29	22632.0
30	29892.0
31	38951.0
32	50181.0
33	68348.0
34	104127.0
35	194218.0
36	656448.0
37	3404097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.38113763527312	22.483359145145783	13.627268741037263	24.508234478543837
2	14.151387226086488	20.98979845386889	42.34199853692696	22.516815783117668
3	17.781318792057085	26.740075266220376	31.24744812416494	24.231157817557598
4	12.614081804225059	18.19592696766476	39.71407418121608	29.4759170468941
5	13.192952691085052	37.716715521249846	35.94185750793629	13.148474279728811
6	29.294637115453405	39.2017037316475	18.9142706854475	12.589388467451599
7	25.756105845588245	33.3260143759959	24.16107265943762	16.75680711897823
8	23.459256320093406	38.38762718317222	21.506245763637637	16.646870733096737
9	25.848711287982297	17.202850875049762	21.807908426595745	35.1405294103722
10-11	24.029602902042598	27.23490443331181	29.409796674152854	19.32569599049274
12-13	24.737728312919526	24.93415653143101	29.528528839623835	20.79958631602563
14-15	22.010091908295422	26.231088802407914	26.971020186639926	24.787799102656738
16-17	21.52633488986055	29.195776896462384	27.7308235173519	21.547064696325165
18-19	21.574049279386752	28.536636376607916	29.311229647814812	20.578084696190516
20-21	22.37264724119246	27.76813007459113	29.060872832388775	20.79834985182764
22-23	22.147040477603227	27.641647286333455	28.915178427929924	21.296133808133398
24-25	21.902321307618518	27.804204306868417	28.998920410432998	21.294553975080063
26-27	21.823215610954307	27.943636015490874	28.917284215975126	21.315864157579693
28-29	21.788261639662643	28.01678203035971	28.931923524578384	21.263032805399263
30-31	22.05509965798982	27.888902809425247	28.85330234273927	21.202695189845663
32-33	21.833484664007752	28.06345424163712	28.769163858964898	21.333897235390232
34-35	21.930883762811746	28.123872453158267	28.763163161500067	21.182080622529917
36-37	21.95484941756056	27.948739621249707	28.781059411896432	21.3153515492933
38-39	21.80805759176013	28.12702705121424	28.873727670603206	21.19118768642242
40-41	22.014100826663494	27.953268158839656	28.804968237476785	21.227662777020065
42-43	21.92181954860929	28.074302986454875	28.59451085443638	21.409366610499458
44-45	22.00074903108931	28.20434200782133	28.438699487710185	21.356209473379177
46-47	22.023921995288397	28.152172909060454	28.254072476181147	21.56983261947
48-49	22.00186870666761	28.22731143108448	28.26472930110417	21.506090561143747
50-51	21.935702585610187	28.34174019769526	28.202769108698945	21.51978810799561
52-53	22.107178826560485	28.278721161728537	28.082410118280833	21.531689893430144
54-55	22.115083747958167	28.194736584823275	28.083378851736583	21.60680081548197
56-57	22.088665482768945	28.28314643390551	28.091339266809978	21.536848816515565
58-59	22.102101733341904	28.371630392149072	28.089233074038734	21.43703480047029
60-61	22.142435063944827	28.374553885676868	28.103814088360497	21.37919696201781
62-63	22.169473157719242	28.451640742992762	28.015355608732325	21.363530490555675
64-65	22.098248163427066	28.46907911411077	27.988850745879112	21.443821976583052
66-67	22.23962869593735	28.66675125680041	27.758198270115113	21.335421777147126
68-69	22.121913994057806	28.342855268064284	28.02829386909385	21.50693686878406
70-71	22.22343953129151	28.102166553643983	28.01233934761955	21.66205456744495
72-73	22.227655641136916	28.371043680864343	27.965736980621042	21.4355636973777
74-75	22.049896106188562	28.454823961107444	27.92801295307582	21.56726697962817
76-77	22.13060938955019	28.126477011922912	28.056165818130925	21.686747780395976
78-79	22.03327275157556	28.131907301265567	28.027368856454483	21.80745109070439
80-81	22.052477630424104	28.18074776095686	28.043491452797674	21.723283155821363
82-83	22.10482573974761	28.024522188752123	28.228197521639224	21.64245454986104
84-85	22.180062104619857	28.01735956262491	28.07785375683399	21.724724575921243
86-87	22.0479766273064	28.106137071109334	28.12878632419178	21.717099977392486
88-89	22.138432833720753	28.019809085331875	28.058222782558772	21.783535298388596
90-91	22.144754552511447	28.110499440950882	28.053625985457604	21.691120021080067
92-93	22.18394749631185	28.094866584851975	28.012638494917347	21.708547423918827
94-95	22.044899364148534	28.110275370193193	28.088480837891012	21.756344427767253
96-97	22.120668637602193	28.228708285113896	28.131477708814167	21.51914536846974
98-99	23.231107391252642	30.111347858518723	28.357369054232397	18.30017569599624
100	29.31831136771621	41.0932792062556	29.588409426028196	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	828.0
1	790.5
2	924.0
3	1198.5
4	1535.0
5	2150.5
6	2674.0
7	3202.5
8	3794.0
9	4112.5
10	4229.0
11	4354.5
12	4847.5
13	6081.0
14	8529.0
15	11375.0
16	13624.0
17	15073.5
18	15482.0
19	15131.5
20	14664.5
21	14882.0
22	16596.0
23	20314.5
24	26140.0
25	33955.5
26	43695.0
27	54491.5
28	65167.5
29	76367.0
30	87205.0
31	97759.5
32	109716.5
33	121985.0
34	133629.0
35	145505.0
36	157526.5
37	167322.0
38	175013.5
39	180774.5
40	186034.0
41	190958.0
42	193503.5
43	195833.5
44	199075.0
45	201312.0
46	200376.0
47	198358.5
48	196001.0
49	190845.5
50	184321.5
51	176961.5
52	166481.0
53	154337.0
54	142974.5
55	130728.5
56	117137.5
57	103525.5
58	90521.5
59	76853.5
60	63117.0
61	50403.5
62	39133.0
63	29959.0
64	22182.0
65	16311.0
66	12078.0
67	9088.5
68	6838.0
69	4984.0
70	3580.5
71	2570.0
72	1795.0
73	1157.5
74	748.5
75	499.5
76	346.5
77	225.5
78	141.0
79	85.0
80	50.5
81	30.5
82	23.5
83	17.0
84	9.5
85	6.0
86	3.5
87	2.5
88	2.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010272601841553913
3	5.429493573759996E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.4295448684014064E-5
22-23	0.0
24-25	1.1945952168407518E-4
26-27	2.0635920882313777E-4
28-29	0.0016622264578214925
30-31	0.0024142847355216324
32-33	0.0020709891480168645
34-35	5.683814845359247E-4
36-37	0.0
38-39	4.4193909173340775E-5
40-41	2.0029227093268433E-4
42-43	0.0018490459091204021
44-45	1.5816619403218613E-4
46-47	5.815630819194319E-4
48-49	0.0010703859316759999
50-51	0.0013239590836252044
52-53	0.0
54-55	0.0029990473891993675
56-57	0.05307077716159754
58-59	0.06905577179930708
60-61	0.045157578450968745
62-63	0.007734823289932925
64-65	0.0
66-67	0.0
68-69	1.6404410616026E-4
70-71	0.001606992201623955
72-73	0.007591029851434911
74-75	0.01172752113394326
76-77	0.013862998066677337
78-79	0.004003156775056902
80-81	0.008193534238613477
82-83	0.045550559556029706
84-85	0.038456484664530804
86-87	0.008986549528060083
88-89	0.0025186368574503597
90-91	0.002207661153923121
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	29.0
20-21	85.0
22-23	224.0
24-25	354.0
26-27	1020.0
28-29	2695.0
30-31	10179.0
32-33	11818.0
34-35	16788.0
36-37	27629.0
38-39	32599.0
40-41	31204.0
42-43	34290.0
44-45	40242.0
46-47	41201.0
48-49	39357.0
50-51	36942.0
52-53	34086.0
54-55	37489.0
56-57	40104.0
58-59	39547.0
60-61	38368.0
62-63	36569.0
64-65	38241.0
66-67	40642.0
68-69	41737.0
70-71	41899.0
72-73	38131.0
74-75	39322.0
76-77	41374.0
78-79	43074.0
80-81	43057.0
82-83	40401.0
84-85	39544.0
86-87	40246.0
88-89	42491.0
90-91	43797.0
92-93	41549.0
94-95	47322.0
96-97	137084.0
98-99	1018488.0
100-101	2233263.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.36548545909807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.07939390226501	79.57195463591798
2	6.459389309197537	11.286553655347019
3	1.412944709004824	3.7032780128721146
4	0.4921505042130024	1.719878708780354
5	0.2147787482611782	0.9382124804067624
6	0.12020498638610276	0.6301060194135684
7	0.06668956229011491	0.4078456189171454
8	0.03993971322794735	0.2791481948205425
9	0.02904821451390089	0.22840302264542728
>10	0.08357816069778841	1.074097540137463
>50	0.001365222478138399	0.08095074699163017
>100	5.169674644042076E-4	0.07957136374998704
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.687189718015993E-5	0.0	0.0	0.0
3	0.0	8.687189718015993E-5	0.0	0.0	0.0
4	0.0	1.520258200652799E-4	0.0	2.1717974295039983E-5	0.0
5	0.0	1.520258200652799E-4	0.0	2.1717974295039983E-5	0.0
6	0.0	1.520258200652799E-4	0.0	2.1717974295039983E-5	0.0
7	0.0	1.520258200652799E-4	0.0	2.1717974295039983E-5	0.0
8	0.0	1.520258200652799E-4	0.0	2.1717974295039983E-5	0.0
9	0.0	2.606156915404798E-4	0.0	2.1717974295039983E-5	0.0
10-11	0.0	2.606156915404798E-4	0.0	2.1717974295039983E-5	0.0
12-13	0.0	2.606156915404798E-4	0.0	2.1717974295039983E-5	0.0
14-15	0.0	3.692055630156797E-4	0.0	2.1717974295039983E-5	2.1717974295039983E-5
16-17	0.0	3.692055630156797E-4	0.0	2.1717974295039983E-5	2.1717974295039983E-5
18-19	0.0	3.800645501631997E-4	0.0	5.429493573759996E-5	2.1717974295039983E-5
20-21	0.0	3.9092353731071973E-4	0.0	8.687189718015993E-5	2.1717974295039983E-5
22-23	0.0	3.9092353731071973E-4	0.0	9.773088432767993E-5	2.1717974295039983E-5
24-25	0.0	4.343594859007997E-4	0.0	1.0858987147519992E-4	2.1717974295039983E-5
26-27	0.0	4.5607746019583965E-4	0.0	1.0858987147519992E-4	2.1717974295039983E-5
28-29	0.0	4.5607746019583965E-4	0.0	2.388977172454398E-4	2.1717974295039983E-5
30-31	0.0	5.429493573759996E-4	0.0	9.664498561292793E-4	2.1717974295039983E-5
32-33	0.0	5.429493573759996E-4	0.0	0.0026930288125849577	2.1717974295039983E-5
34-35	0.0	6.515392288511995E-4	0.0	0.004951698139269116	2.1717974295039983E-5
36-37	0.0	7.601291003263994E-4	0.0	0.008350561116442874	2.1717974295039983E-5
38-39	0.0	7.601291003263994E-4	0.0	0.013291400268564471	3.257696144255997E-5
40-41	0.0	7.818470746214395E-4	0.0	0.019209548263962865	4.3435948590079966E-5
42-43	0.0	8.361420103590394E-4	0.0	0.025323158028016622	4.3435948590079966E-5
44-45	0.0	9.447318818342394E-4	0.0	0.0313390369077427	4.3435948590079966E-5
46-47	0.0	9.990268175718393E-4	0.0	0.03795216008058237	4.3435948590079966E-5
48-49	0.0	0.0010098858047193592	0.0	0.04393546199886589	4.3435948590079966E-5
50-51	0.0	0.0011076166890470391	0.0	0.0512218423748518	4.3435948590079966E-5
52-53	0.0	0.0011076166890470391	0.0	0.057867542509134035	4.3435948590079966E-5
54-55	0.0	0.0011184756761945592	0.0	0.06458925555344891	4.3435948590079966E-5
56-57	0.0	0.0011293346633420792	0.0	0.0712023787262886	4.3435948590079966E-5
58-59	0.0	0.0011293346633420792	0.0	0.07824986138502907	4.3435948590079966E-5
60-61	0.0	0.0011619116247846392	0.0	0.0844069070976729	4.3435948590079966E-5
62-63	0.0	0.001194488586227199	0.0	0.09125892798775802	4.3435948590079966E-5
64-65	0.0	0.0012813604834073591	0.0	0.09730738382892665	4.3435948590079966E-5
66-67	0.0	0.0012813604834073591	0.0	0.10359473738734072	4.3435948590079966E-5
68-69	0.0	0.0012922194705548792	0.0	0.11024043752162296	4.3435948590079966E-5
70-71	0.0	0.001303078457702399	0.0	0.11562649514679288	4.3435948590079966E-5
72-73	0.0	0.0013139374448499189	0.0	0.12035015455596407	1.0858987147519992E-4
74-75	0.0	0.0013790913677350389	0.0	0.12398791525038327	1.0858987147519992E-4
76-77	0.0	0.0014876812392102389	0.0	0.1272130344331967	1.0858987147519992E-4
78-79	0.0	0.0015419761749478388	0.0	0.1296020116056511	1.0858987147519992E-4
80-81	0.0	0.0015528351620953589	0.0	0.13021011488591222	1.0858987147519992E-4
82-83	0.0	0.0015745531363903988	0.0	0.13065533335896054	1.303078457702399E-4
84-85	0.0	0.0015854121235379188	0.0	0.13089423107620599	1.303078457702399E-4
86-87	0.0	0.0015854121235379188	0.0	0.13104625689627125	1.303078457702399E-4
88	0.0	0.0016288480721279987	0.0	0.13104625689627125	1.303078457702399E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4945	0.0	46.708626	1
GTATCAA	9390	0.0	37.59384	1
TCAACGC	10730	0.0	32.304493	4
ATCAACG	10815	0.0	32.129017	3
CAACGCA	10910	0.0	31.77151	5
AACGCAG	11230	0.0	31.091755	6
TATCAAC	11735	0.0	29.90157	2
ACGCAGA	13080	0.0	26.629663	7
CGCAGAG	13440	0.0	25.884954	8
GCAGAGT	15905	0.0	21.89978	9
GAGTACT	9040	0.0	19.358673	12-13
GTACATG	12295	0.0	18.717335	1
AGAGTAC	14195	0.0	18.470373	10-11
CAGAGTA	15690	0.0	18.432621	10-11
TACATGG	12365	0.0	18.133284	2
ACATGGG	12285	0.0	17.699306	3
TATACCG	315	1.551598E-9	17.424175	5
GTACTTT	10235	0.0	16.871914	14-15
AGTACTT	10040	0.0	15.91665	12-13
CATGGGG	8675	0.0	15.427985	4
>>END_MODULE