FastQCFastQC Report
Fri 27 May 2016
SRR2138677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4083861
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47200.051.647661
GTATCAA90650.038.350481
TCAACGC107100.032.1813744
ATCAACG108600.031.6978423
CAACGCA110750.031.120775
TATCAAC113450.030.493722
AACGCAG114100.030.2070546
ACGCAGA129250.026.7647447
CGCAGAG138600.025.0203598
CGTCGAA2101.2148998E-424.98021594
GCAGAGT156550.022.205699
GTACATG129650.021.2552191
TACATGG133450.020.6182062
ACATGGG132700.020.3184093
GAGTACT84350.019.72686612-13
CAGAGTA152800.019.37967710-11
AGAGTAC143600.018.56947910-11
CATGGGG92450.018.0214254
GTGGTAT25300.017.7627451
TGGTATC22300.017.6808382
Produced by FastQC (version 0.11.5)