##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138677_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4083861
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.521683524488225	30.0	18.0	33.0	18.0	33.0
2	31.258010005727424	32.0	31.0	33.0	27.0	34.0
3	31.481097667134115	33.0	31.0	33.0	29.0	34.0
4	32.7273151559272	33.0	33.0	33.0	32.0	34.0
5	32.65643100977237	33.0	33.0	33.0	32.0	34.0
6	36.94781898796262	38.0	37.0	38.0	35.0	38.0
7	37.0035899850656	38.0	38.0	38.0	35.0	38.0
8	37.34557077236468	38.0	38.0	38.0	37.0	38.0
9	37.43799360458155	38.0	38.0	38.0	37.0	38.0
10-11	37.45064303119034	38.0	38.0	38.0	37.0	38.0
12-13	37.48038522858638	38.0	38.0	38.0	37.0	38.0
14-15	37.48894195958188	38.0	38.0	38.0	37.0	38.0
16-17	37.52008111931332	38.0	38.0	38.0	37.5	38.0
18-19	37.52121815605379	38.0	38.0	38.0	38.0	38.0
20-21	37.52729954263321	38.0	38.0	38.0	38.0	38.0
22-23	37.537735049487885	38.0	38.0	38.0	38.0	38.0
24-25	37.549088144709785	38.0	38.0	38.0	38.0	38.0
26-27	37.51954250511481	38.0	38.0	38.0	38.0	38.0
28-29	37.494245260008896	38.0	38.0	38.0	37.0	38.0
30-31	37.49862650085422	38.0	38.0	38.0	38.0	38.0
32-33	37.48633658628659	38.0	38.0	38.0	37.5	38.0
34-35	37.385585310342435	38.0	38.0	38.0	37.0	38.0
36-37	37.42995188904582	38.0	38.0	38.0	37.0	38.0
38-39	37.437467666515374	38.0	38.0	38.0	37.0	38.0
40-41	37.43351532903272	38.0	38.0	38.0	37.0	38.0
42-43	37.40216291841841	38.0	38.0	38.0	37.0	38.0
44-45	37.37058422023768	38.0	38.0	38.0	37.0	38.0
46-47	37.34331785584739	38.0	38.0	38.0	37.0	38.0
48-49	37.287027753275986	38.0	38.0	38.0	37.0	38.0
50-51	37.25527766400948	38.0	38.0	38.0	37.0	38.0
52-53	37.22898464155908	38.0	38.0	38.0	37.0	38.0
54-55	37.19738763310504	38.0	38.0	38.0	37.0	38.0
56-57	37.15113513502209	38.0	38.0	38.0	36.0	38.0
58-59	37.14558335151416	38.0	38.0	38.0	36.0	38.0
60-61	37.14559970350092	38.0	38.0	38.0	36.0	38.0
62-63	37.151013576877396	38.0	38.0	38.0	36.0	38.0
64-65	37.15758521277401	38.0	38.0	38.0	36.0	38.0
66-67	37.15350239908719	38.0	38.0	38.0	36.0	38.0
68-69	37.143602369969486	38.0	38.0	38.0	36.0	38.0
70-71	37.13987121541922	38.0	38.0	38.0	36.0	38.0
72-73	37.1294437768359	38.0	38.0	38.0	36.0	38.0
74-75	37.12590008313921	38.0	38.0	38.0	36.0	38.0
76-77	37.109143420765776	38.0	38.0	38.0	36.0	38.0
78-79	37.09280234306812	38.0	38.0	38.0	36.0	38.0
80-81	37.080022948334204	38.0	38.0	38.0	36.0	38.0
82-83	37.03040513483096	38.0	38.0	38.0	36.0	38.0
84-85	37.01735920147722	38.0	38.0	38.0	36.0	38.0
86-87	37.001426443781284	38.0	38.0	38.0	36.0	38.0
88-89	36.9882898514891	38.0	38.0	38.0	36.0	38.0
90-91	36.975038365703696	38.0	38.0	38.0	36.0	38.0
92-93	36.95363722047485	38.0	38.0	38.0	35.0	38.0
94-95	36.936029033611206	38.0	38.0	38.0	35.0	38.0
96-97	36.92482653379285	38.0	38.0	38.0	35.0	38.0
98-99	36.94217521289236	38.0	38.0	38.0	35.0	38.0
100	35.492630296895335	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	4.0
21	45.0
22	170.0
23	539.0
24	1367.0
25	2741.0
26	5069.0
27	8639.0
28	13956.0
29	20449.0
30	27599.0
31	36362.0
32	46692.0
33	62833.0
34	94208.0
35	174419.0
36	577992.0
37	3010774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.0487430889543	22.05427657797364	13.498329154689642	24.39865117838242
2	14.476310030006584	21.329703384673255	41.39934080040907	22.794645784911094
3	17.896758164947652	26.69296388266992	31.01562283827261	24.394655114109824
4	12.827615827277178	18.3918355693301	39.02094121224987	29.759607391142843
5	13.585599509875582	37.523755093525466	35.46318055389251	13.42746484270645
6	29.60083607155092	38.787265286453184	18.726225011086324	12.885673630909572
7	26.121334687933796	33.24988289268415	23.726395193176263	16.90238722620579
8	24.080814699618816	37.71286534972664	21.118544436257746	17.087775514396792
9	26.195014962556268	17.020975983266815	21.418775026867955	35.365234027308965
10-11	24.333773847836643	27.14665117152616	28.800343596415257	19.719231384221942
12-13	25.14518736068637	24.954644636533907	28.850222865077924	21.049945137701798
14-15	22.413189381323214	25.992473788897318	26.558225169759698	25.03611166001977
16-17	21.883923081613208	29.124999602092235	27.14868356195277	21.842393754341785
18-19	21.947049617996303	28.344990194328357	28.726663321792785	20.981296865882555
20-21	22.935547078738573	27.45727160221146	28.54292233395318	21.06425898509679
22-23	22.67072931380876	27.34381016666591	28.40222312635684	21.58323739316849
24-25	22.3876952915744	27.49656389682568	28.57595027767387	21.53979053392605
26-27	22.251429626659817	27.624827365952832	28.52552461279644	21.59821839459092
28-29	22.331073284101272	27.700678798771666	28.468412802557413	21.49983511456965
30-31	22.592044505876665	27.430886301454528	28.42323844802334	21.55383074464547
32-33	22.430147952920798	27.534601754023125	28.32279019617891	21.71246009687717
34-35	22.562497704985745	27.678426422397468	28.232298077560515	21.526777795056272
36-37	22.65470022489659	27.455063365214098	28.210368502303524	21.679867907585788
38-39	22.295850605932337	27.79223669303042	28.323847120525453	21.588065580511792
40-41	22.48272461137423	27.575108506003694	28.321451400761706	21.620715481860373
42-43	22.383825572311213	27.718148756644727	28.032888076962177	21.865137594081883
44-45	22.37715420264949	27.75421434364029	27.98619073162175	21.88244072208847
46-47	22.48070058350324	27.770178364903312	27.84363888103013	21.905482170563314
48-49	22.576716807271282	27.951270294069502	27.642132083150283	21.82988081550893
50-51	22.52803768873046	28.005007622763955	27.666998306821043	21.799956381684535
52-53	22.75147485629698	27.870550778355994	27.58459857818577	21.793375787161253
54-55	22.741929152549744	27.878074889571064	27.497428585220586	21.882567372658606
56-57	22.703385318955466	28.10412152499785	27.326110844803058	21.866382311243626
58-59	22.620758734429977	28.033394434116833	27.546989133213945	21.79885769823925
60-61	22.70170502924522	28.139206673901107	27.42028923855807	21.738799058295605
62-63	22.66307836242643	28.124407014023756	27.594232862464658	21.61828176108516
64-65	22.864934176450337	28.035993842245976	27.41944164629202	21.67963033501167
66-67	22.87414182516267	28.39124511139645	27.13203325300598	21.602579810434904
68-69	22.834822407250517	27.946860074196756	27.3889134828489	21.829404035703828
70-71	22.846726032237154	27.897416019785474	27.34233593000534	21.913522017972035
72-73	22.81869668341569	28.197940964377683	27.174386726616223	21.808975625590403
74-75	22.61589073754722	28.157890286155514	27.24870633120604	21.97751264509123
76-77	22.730134255906947	27.770283765622782	27.423140304993694	22.07644167347658
78-79	22.55691508090528	27.834736902440966	27.47332995375033	22.13501806290342
80-81	22.654107121968227	27.804643556827408	27.519259579430493	22.02198974177387
82-83	22.772335578047194	27.556544114254265	27.66002095120416	22.01109935649438
84-85	22.711018444819132	27.58383211231746	27.63583378425046	22.069315658612947
86-87	22.537908653464847	27.761485187531704	27.559675823589657	22.14093033541379
88-89	22.789885783389447	27.575393499261676	27.417128874644792	22.217591842704085
90-91	22.81099657779349	27.605077718607507	27.465009637339687	22.118916066259317
92-93	22.784305729102744	27.670927471609936	27.48955253978056	22.055214259506762
94-95	22.506865054962827	27.821698986169462	27.541015891192057	22.13042006767565
96-97	22.640115293392817	27.883664631710225	27.577866041525574	21.898354033371387
98-99	23.755900343509133	29.761788202123203	27.776126052512453	18.706185401855212
100	30.087736721276105	40.780687435661925	29.131575843061974	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	864.0
1	797.5
2	881.0
3	1108.5
4	1350.5
5	1791.5
6	2236.0
7	2725.0
8	3368.0
9	3830.5
10	3967.0
11	4114.0
12	4623.5
13	5815.5
14	8119.5
15	10805.5
16	12805.5
17	13930.5
18	14084.5
19	13540.5
20	12794.0
21	12603.5
22	13797.0
23	16644.0
24	21139.5
25	27482.0
26	35427.0
27	44231.0
28	53073.5
29	62480.5
30	71562.5
31	80189.0
32	89868.0
33	99726.0
34	108767.0
35	117180.5
36	126181.0
37	134166.0
38	140400.0
39	145726.0
40	150994.5
41	155585.0
42	158210.5
43	160689.5
44	164532.0
45	169025.5
46	173272.5
47	177867.0
48	179771.0
49	176319.0
50	170099.0
51	166119.5
52	157559.5
53	144684.0
54	134740.5
55	125146.5
56	114426.5
57	102690.0
58	90871.5
59	77993.0
60	65808.0
61	55308.5
62	45048.0
63	36763.0
64	29530.5
65	22298.5
66	16896.0
67	11790.0
68	7765.5
69	5664.5
70	4071.0
71	2891.5
72	2102.0
73	1372.0
74	843.5
75	553.0
76	369.0
77	231.5
78	143.5
79	78.0
80	39.0
81	20.0
82	11.5
83	9.0
84	7.5
85	6.0
86	4.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010382331817855701
3	7.835722126683548E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.346061445886095E-5
22-23	0.0
24-25	9.795562928349252E-5
26-27	2.3266815141602447E-4
28-29	0.001629227708091884
30-31	0.0023298138429690756
32-33	0.002150485837760467
34-35	5.544970139103585E-4
36-37	1.2371908986793854E-5
38-39	2.4892201211130048E-5
40-41	1.5034583300236367E-4
42-43	0.0017153087013322145
44-45	1.9066516083559643E-4
46-47	3.719181292774593E-4
48-49	9.704956385926001E-4
50-51	0.00105716523101018
52-53	0.0
54-55	0.002943977695788441
56-57	0.05635027609896082
58-59	0.07317815890558207
60-61	0.04888287570312572
62-63	0.007589644425158682
64-65	0.0
66-67	0.0
68-69	9.900434162324972E-5
70-71	0.0014864039771596879
72-73	0.0076813010853447414
74-75	0.012230985737271136
76-77	0.013195837420213784
78-79	0.0038111906382865958
80-81	0.00820434200876013
82-83	0.04864622398907256
84-85	0.04175010791341452
86-87	0.00937656130241836
88-89	0.002185840815120483
90-91	0.0028168789935625565
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	24.0
20-21	89.0
22-23	215.0
24-25	315.0
26-27	1019.0
28-29	2679.0
30-31	8279.0
32-33	10304.0
34-35	14217.0
36-37	22829.0
38-39	26739.0
40-41	26006.0
42-43	29150.0
44-45	34221.0
46-47	35333.0
48-49	33458.0
50-51	31408.0
52-53	29420.0
54-55	32186.0
56-57	34683.0
58-59	33959.0
60-61	32823.0
62-63	31749.0
64-65	33294.0
66-67	35338.0
68-69	36494.0
70-71	36354.0
72-73	32991.0
74-75	34387.0
76-77	36047.0
78-79	37107.0
80-81	36991.0
82-83	34907.0
84-85	34609.0
86-87	35378.0
88-89	37515.0
90-91	37798.0
92-93	36861.0
94-95	41484.0
96-97	123111.0
98-99	928428.0
100-101	1983662.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.12382991170598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.03140521673329	83.84346540199867
2	5.216507752940794	9.402633149182634
3	1.0373396364169176	2.804670628593276
4	0.3310090285603676	1.1932720555685439
5	0.14417323194905135	0.649672191699862
6	0.08343253382856616	0.4511555692721011
7	0.04156952339194543	0.2622483258980462
8	0.02753769663757929	0.19854421503402828
9	0.01970853202681607	0.15985875493747637
>10	0.06542706840316105	0.895616127557162
>50	0.0014772366874074134	0.0839411608087439
>100	4.1254242411745205E-4	0.05492241944948852
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.8973263291772174E-5	0.0	0.0	0.0
3	0.0	4.8973263291772174E-5	0.0	0.0	0.0
4	0.0	1.4691978987531651E-4	0.0	0.0	0.0
5	0.0	1.4691978987531651E-4	0.0	0.0	2.4486631645886087E-5
6	2.4486631645886087E-5	1.4691978987531651E-4	0.0	0.0	2.4486631645886087E-5
7	2.4486631645886087E-5	1.4691978987531651E-4	0.0	0.0	4.8973263291772174E-5
8	2.4486631645886087E-5	1.4691978987531651E-4	0.0	0.0	4.8973263291772174E-5
9	2.4486631645886087E-5	1.958930531670887E-4	0.0	0.0	4.8973263291772174E-5
10-11	2.4486631645886087E-5	1.958930531670887E-4	0.0	2.4486631645886087E-5	4.8973263291772174E-5
12-13	2.4486631645886087E-5	1.958930531670887E-4	0.0	4.8973263291772174E-5	4.8973263291772174E-5
14-15	4.8973263291772174E-5	4.774893170947787E-4	0.0	4.8973263291772174E-5	6.121657911471521E-5
16-17	4.8973263291772174E-5	4.897326329177217E-4	0.0	7.345989493765826E-5	7.345989493765826E-5
18-19	4.8973263291772174E-5	5.019759487406648E-4	0.0	8.57032107606013E-5	8.57032107606013E-5
20-21	4.8973263291772174E-5	5.387058962094939E-4	0.0	9.794652658354435E-5	9.794652658354435E-5
22-23	4.8973263291772174E-5	5.6319252785538E-4	0.0	9.794652658354435E-5	9.794652658354435E-5
24-25	6.121657911471521E-5	5.6319252785538E-4	0.0	9.794652658354435E-5	9.794652658354435E-5
26-27	7.345989493765826E-5	5.6319252785538E-4	0.0	1.4691978987531651E-4	1.2243315822943042E-4
28-29	7.345989493765826E-5	5.6319252785538E-4	0.0	3.7954279051123435E-4	1.2243315822943042E-4
30-31	7.345989493765826E-5	5.6319252785538E-4	0.0	0.0011631150031795891	1.2243315822943042E-4
32-33	8.57032107606013E-5	5.6319252785538E-4	0.0	0.0032322353772569635	1.2243315822943042E-4
34-35	9.794652658354435E-5	6.121657911471521E-4	0.0	0.005607438646907914	1.2243315822943042E-4
36-37	9.794652658354435E-5	6.733823702618673E-4	0.0	0.009562029657718516	1.2243315822943042E-4
38-39	9.794652658354435E-5	7.713288968454117E-4	0.0	0.015402091305262348	1.2243315822943042E-4
40-41	9.794652658354435E-5	9.060053708977852E-4	0.0	0.021805345480661563	1.2243315822943042E-4
42-43	9.794652658354435E-5	9.427353183666143E-4	0.0	0.028024949918716627	1.2243315822943042E-4
44-45	9.794652658354435E-5	0.0010651684765960448	0.0	0.03477101693715824	1.2243315822943042E-4
46-47	9.794652658354435E-5	0.0011508716873566461	0.0	0.04183541016699638	1.2243315822943042E-4
48-49	9.794652658354435E-5	0.0011508716873566461	0.0	0.049009993239241004	1.2243315822943042E-4
50-51	1.1018984240648738E-4	0.0012977914772319627	0.0	0.05607438646907914	1.2243315822943042E-4
52-53	1.2243315822943042E-4	0.0012977914772319627	0.0	0.06282045348752076	1.3467647405237347E-4
54-55	1.2243315822943042E-4	0.0013590080563466778	0.0	0.06870948839835636	1.4691978987531651E-4
56-57	1.2243315822943042E-4	0.001395738003815507	0.0	0.07568817841743389	1.4691978987531651E-4
58-59	1.2243315822943042E-4	0.001395738003815507	0.0	0.08293622138461618	1.5916310569825956E-4
60-61	1.2243315822943042E-4	0.0014691978987531652	0.0	0.09041688735243437	1.714064215212026E-4
62-63	1.2243315822943042E-4	0.0014936845303990514	0.0	0.09749352389809546	1.714064215212026E-4
64-65	1.2243315822943042E-4	0.001665090951920254	0.0	0.10452118718046476	1.714064215212026E-4
66-67	1.3467647405237347E-4	0.00168957758356614	0.0	0.11181820341093882	1.8364973734414565E-4
68-69	1.4691978987531651E-4	0.001714064215212026	0.0	0.11760929179519088	1.958930531670887E-4
70-71	1.4691978987531651E-4	0.001738550846857912	0.0	0.12303308070475465	1.958930531670887E-4
72-73	1.4691978987531651E-4	0.0017507941626808553	0.0	0.12750189098012885	1.958930531670887E-4
74-75	1.4691978987531651E-4	0.0018364973734414564	0.0	0.13057496325168755	1.958930531670887E-4
76-77	1.4691978987531651E-4	0.0018854706367332286	0.0	0.1340275783137575	1.958930531670887E-4
78-79	1.4691978987531651E-4	0.0018977139525561716	0.0	0.13653745805746081	1.958930531670887E-4
80-81	1.4691978987531651E-4	0.0019099572683791148	0.0	0.1372965436384833	1.958930531670887E-4
82-83	1.4691978987531651E-4	0.0019344439000250008	0.0	0.13796992600874514	1.958930531670887E-4
84-85	1.4691978987531651E-4	0.001958930531670887	0.0	0.13831273885178758	1.958930531670887E-4
86-87	1.4691978987531651E-4	0.001983417163316773	0.0	0.13843517201001698	2.0813636899003174E-4
88	1.4691978987531651E-4	0.0021058503215462035	0.0	0.13844741532583993	2.2037968481297479E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4720	0.0	51.64766	1
GTATCAA	9065	0.0	38.35048	1
TCAACGC	10710	0.0	32.181374	4
ATCAACG	10860	0.0	31.697842	3
CAACGCA	11075	0.0	31.12077	5
TATCAAC	11345	0.0	30.49372	2
AACGCAG	11410	0.0	30.207054	6
ACGCAGA	12925	0.0	26.764744	7
CGCAGAG	13860	0.0	25.020359	8
CGTCGAA	210	1.2148998E-4	24.980215	94
GCAGAGT	15655	0.0	22.20569	9
GTACATG	12965	0.0	21.255219	1
TACATGG	13345	0.0	20.618206	2
ACATGGG	13270	0.0	20.318409	3
GAGTACT	8435	0.0	19.726866	12-13
CAGAGTA	15280	0.0	19.379677	10-11
AGAGTAC	14360	0.0	18.569479	10-11
CATGGGG	9245	0.0	18.021425	4
GTGGTAT	2530	0.0	17.762745	1
TGGTATC	2230	0.0	17.680838	2
>>END_MODULE