FastQCFastQC Report
Fri 27 May 2016
SRR2138676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5216880
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58800.049.37631
GTATCAA109600.038.111461
TCAACGC122500.032.869024
ATCAACG124800.032.22893
TATCAAC128450.031.650562
CAACGCA127450.031.5587835
AACGCAG131450.030.6310776
ACGCAGA149400.027.036947
CGCAGAG152750.026.4439858
GCAGAGT185950.021.6764879
GTACATG144550.021.2719961
TACATGG139950.021.2663882
ACATGGG140750.020.198653
GAGTACT113800.019.78218712-13
CAGAGTA182450.018.96648810-11
GATCAAC22600.018.7859861
AGAGTAC166400.018.66991410-11
CATGGGG101100.018.153064
GTGGTAT32300.017.9241451
GTACTTT128800.017.42858914-15
Produced by FastQC (version 0.11.5)