##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138676_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5216880
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.706600880219593	28.0	18.0	33.0	18.0	33.0
2	30.5396190826701	32.0	27.0	33.0	27.0	33.0
3	28.692502798607595	31.0	28.0	33.0	18.0	33.0
4	31.98309775191302	33.0	32.0	33.0	31.0	33.0
5	32.73452101639294	33.0	33.0	33.0	32.0	34.0
6	37.086538122402665	38.0	37.0	38.0	36.0	38.0
7	37.0855842955943	38.0	38.0	38.0	35.0	38.0
8	37.37354740764595	38.0	38.0	38.0	37.0	38.0
9	37.48015978899265	38.0	38.0	38.0	37.0	38.0
10-11	37.44701286976124	38.0	38.0	38.0	37.0	38.0
12-13	37.55491606094064	38.0	38.0	38.0	37.5	38.0
14-15	37.52975034886752	38.0	38.0	38.0	37.5	38.0
16-17	37.571593461992606	38.0	38.0	38.0	38.0	38.0
18-19	37.56151703700296	38.0	38.0	38.0	38.0	38.0
20-21	37.5653113513888	38.0	38.0	38.0	38.0	38.0
22-23	37.57306599020394	38.0	38.0	38.0	38.0	38.0
24-25	37.579516444561364	38.0	38.0	38.0	38.0	38.0
26-27	37.544648286763426	38.0	38.0	38.0	38.0	38.0
28-29	37.52043729018867	38.0	38.0	38.0	38.0	38.0
30-31	37.52343389546413	38.0	38.0	38.0	38.0	38.0
32-33	37.51161635858635	38.0	38.0	38.0	38.0	38.0
34-35	37.406787680385996	38.0	38.0	38.0	37.0	38.0
36-37	37.44824249081238	38.0	38.0	38.0	37.0	38.0
38-39	37.45514691542991	38.0	38.0	38.0	37.0	38.0
40-41	37.45067111581807	38.0	38.0	38.0	37.5	38.0
42-43	37.41816777585345	38.0	38.0	38.0	37.0	38.0
44-45	37.389146807192766	38.0	38.0	38.0	37.0	38.0
46-47	37.36464117713669	38.0	38.0	38.0	37.0	38.0
48-49	37.30995112837656	38.0	38.0	38.0	37.0	38.0
50-51	37.28426169837533	38.0	38.0	38.0	37.0	38.0
52-53	37.266627618502085	38.0	38.0	38.0	37.0	38.0
54-55	37.2406115207623	38.0	38.0	38.0	37.0	38.0
56-57	37.199386016584626	38.0	38.0	38.0	37.0	38.0
58-59	37.19404935210819	38.0	38.0	38.0	37.0	38.0
60-61	37.19486801643737	38.0	38.0	38.0	37.0	38.0
62-63	37.20264850793933	38.0	38.0	38.0	37.0	38.0
64-65	37.209897911643495	38.0	38.0	38.0	37.0	38.0
66-67	37.209527655368035	38.0	38.0	38.0	37.0	38.0
68-69	37.2021529583247	38.0	38.0	38.0	37.0	38.0
70-71	37.19699538689921	38.0	38.0	38.0	36.0	38.0
72-73	37.18880416106694	38.0	38.0	38.0	36.0	38.0
74-75	37.18763350094925	38.0	38.0	38.0	36.0	38.0
76-77	37.17528516152831	38.0	38.0	38.0	36.0	38.0
78-79	37.1621180158819	38.0	38.0	38.0	36.0	38.0
80-81	37.15665716293968	38.0	38.0	38.0	36.0	38.0
82-83	37.11051604326957	38.0	38.0	38.0	36.0	38.0
84-85	37.103568671273095	38.0	38.0	38.0	36.0	38.0
86-87	37.091096063326155	38.0	38.0	38.0	36.0	38.0
88-89	37.07672956043764	38.0	38.0	38.0	36.0	38.0
90-91	37.06922602942079	38.0	38.0	38.0	36.0	38.0
92-93	37.054813490455075	38.0	38.0	38.0	36.0	38.0
94-95	37.04184998285277	38.0	38.0	38.0	36.0	38.0
96-97	37.033324123737756	38.0	38.0	38.0	36.0	38.0
98-99	37.048096179460515	38.0	38.0	38.0	36.0	38.0
100	35.7081681670251	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	10.0
21	47.0
22	233.0
23	684.0
24	1632.0
25	3451.0
26	6439.0
27	10761.0
28	17479.0
29	25560.0
30	34142.0
31	43979.0
32	56483.0
33	76097.0
34	115548.0
35	219942.0
36	782614.0
37	3821773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.6619742068056	17.587101869316527	11.562715646133322	23.18820827774455
2	14.5374070163412	20.415400493970296	42.579921280672814	22.467271209015685
3	19.06538663031254	26.135732593216073	30.145408198962166	24.65347257750922
4	12.614397877658678	17.624231341338117	39.96526659612642	29.796104184876786
5	13.474490500069006	37.775988713560594	35.56813651071138	13.181384275659013
6	29.69088804036129	37.99167318397203	19.329905997454418	12.987532778212266
7	26.43131526889635	32.86199797580163	23.752568585054668	16.95411817024735
8	23.92966677401052	37.43637959853399	21.612036312892	17.021917314563495
9	26.13941666283296	17.148372207142966	21.535496311971908	35.17671481805217
10-11	24.24560273573477	26.729050696968304	29.34696600266826	19.678380564628668
12-13	25.20591809664014	24.40996342641579	29.394034365367805	20.99008411157627
14-15	22.354338225146066	25.64339221910414	27.036849611261903	24.965419944487895
16-17	21.861275321648186	28.72471668890218	27.67471170508043	21.739296284369203
18-19	21.912541595743047	27.9363910996611	29.320283387772	20.83078391682385
20-21	22.66191128885633	27.190666823466852	29.18178959427568	20.965632293401136
22-23	22.414885915675065	27.082740883849805	29.036489520796664	21.465883679678463
24-25	22.176955136029864	27.162638397614163	29.130328471690454	21.530077994665525
26-27	22.109202844008593	27.3133739185716	29.10108822477223	21.476335012647578
28-29	22.105317964834818	27.373517492457534	29.11778671529028	21.403377827417366
30-31	22.34842072574104	27.232322964781623	28.982359143967827	21.436897165509507
32-33	22.121201809350673	27.427133415772296	28.95150443417033	21.5001603407067
34-35	22.28191606145129	27.42605168364468	28.912305932001814	21.37972632290221
36-37	22.248250531318686	27.34110045912468	28.927213230229142	21.483435779327493
38-39	22.067686061391942	27.49375080436994	29.03736404630257	21.401199087935545
40-41	22.359770396473913	27.332745808317622	28.86439305423551	21.443090740972956
42-43	22.24717750571707	27.406233640178247	28.74322296455216	21.603365889552524
44-45	22.26791720777632	27.497984398448327	28.64399855811718	21.590099835658176
46-47	22.34340847591423	27.536688235416545	28.406359270902758	21.71354401776646
48-49	22.27211514659479	27.612838800366323	28.406356044831988	21.708690008206897
50-51	22.23339542954496	27.76620528665898	28.363968508941884	21.63643077485418
52-53	22.454072845326497	27.635808581210767	28.237443697527397	21.67267487593534
54-55	22.402532264506554	27.544811273872416	28.262092901204017	21.790563560417013
56-57	22.406682936110585	27.63912781291944	28.275509873120235	21.678679377849743
58-59	22.39409858146108	27.660857993314806	28.279894100924913	21.6651493242992
60-61	22.47892555779421	27.577591429324364	28.29550310407714	21.64797990880428
62-63	22.447760707655696	27.603775529270063	28.332900602458714	21.615563160615523
64-65	22.467039420242312	27.569545231608416	28.253322559319553	21.710092788829723
66-67	22.64650783301752	27.552412458400976	28.165521472313458	21.635558236268047
68-69	22.529260531484685	27.540541405992624	28.264037049680894	21.666161012841798
70-71	22.431139235262393	27.525554106581396	28.311298977265842	21.732007680890373
72-73	22.635337462002507	27.609555868417342	28.151010823235247	21.60409584634491
74-75	22.283513392272	27.691957982699783	28.212473939033693	21.81205468599452
76-77	22.396174811045086	27.506426213768382	28.17174359153509	21.925655383651442
78-79	22.32443963680168	27.668384937409918	28.076331508085584	21.930843917702816
80-81	22.36821148857342	27.63737154003158	28.080161481196463	21.914255490198535
82-83	22.347627461465137	27.6382489014949	28.11339595006161	21.900727686978357
84-85	22.432856859120427	27.57215548745129	28.090927488365956	21.904060165062326
86-87	22.27414784308595	27.599500471182047	28.13317102675973	21.99318065897227
88-89	22.334710469190103	27.648962909292596	28.05308720427531	21.96323941724199
90-91	22.383772213140567	27.629322562401175	28.086217019236788	21.900688205221467
92-93	22.38259240399328	27.678559075488067	28.03004080968991	21.90880771082874
94-95	22.33065357415373	27.731191309440785	28.031640900132558	21.906514216272928
96-97	22.434887507747217	27.81830781878341	28.058795568301715	21.688009105167655
98-99	23.437435884196837	29.77080680948894	28.298212026799895	18.493545279514333
100	29.721903964372036	40.655843785090305	29.622252250537656	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	621.0
1	654.0
2	1058.0
3	1590.0
4	2004.5
5	2796.0
6	3578.0
7	4369.5
8	5546.5
9	6276.0
10	6146.0
11	6019.0
12	6529.0
13	7843.5
14	10159.0
15	12873.0
16	15473.0
17	17551.5
18	18191.5
19	17401.0
20	16360.0
21	15984.5
22	17097.0
23	20301.0
24	25427.5
25	32775.0
26	42720.0
27	54133.5
28	65528.0
29	77657.0
30	89899.0
31	101695.0
32	113644.5
33	126560.5
34	139520.0
35	150278.5
36	162273.0
37	173043.0
38	180569.0
39	187934.5
40	196054.0
41	203256.5
42	207379.0
43	211298.5
44	215322.5
45	217924.5
46	218333.0
47	217696.5
48	216523.0
49	213448.5
50	208963.5
51	201978.5
52	192523.5
53	182337.5
54	170490.5
55	157083.5
56	142659.0
57	127710.0
58	113213.0
59	97372.5
60	80956.5
61	65714.0
62	51905.5
63	39593.0
64	29445.5
65	21689.5
66	15716.0
67	11982.5
68	9167.5
69	6701.5
70	4887.5
71	3448.0
72	2343.5
73	1598.0
74	1077.0
75	699.5
76	448.0
77	287.5
78	182.0
79	112.5
80	77.0
81	51.0
82	31.5
83	24.0
84	17.5
85	11.5
86	7.5
87	4.5
88	3.0
89	2.5
90	3.5
91	4.0
92	4.0
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007130698808483231
3	9.392587140206408E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	4.7921794698047654E-5
22-23	0.0
24-25	1.0543564859793592E-4
26-27	2.4923164758798404E-4
28-29	0.001725934622363587
30-31	0.0026869761116308064
32-33	0.0021628224660155708
34-35	4.72050833516534E-4
36-37	0.0
38-39	9.71314555750833E-6
40-41	2.1485243641686296E-4
42-43	0.0020425576710757436
44-45	2.569842131632651E-4
46-47	4.5808114389633267E-4
48-49	9.331632367399009E-4
50-51	9.501853215169365E-4
52-53	0.0
54-55	0.0027972002177512785
56-57	0.050392240771875735
58-59	0.06623935827343012
60-61	0.04334274350121213
62-63	0.007109400341124263
64-65	0.0
66-67	0.0
68-69	1.5196728708758927E-4
70-71	0.0014575987802638057
72-73	0.007036129084647177
74-75	0.011185677331834171
76-77	0.012439557160779248
78-79	0.0036416378038420416
80-81	0.007060221504675787
82-83	0.04468743544083121
84-85	0.03704476854998891
86-87	0.008614684137925478
88-89	0.002095415398740787
90-91	0.0024578586215505526
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	29.0
20-21	112.0
22-23	224.0
24-25	327.0
26-27	1062.0
28-29	2825.0
30-31	8340.0
32-33	10629.0
34-35	14616.0
36-37	24087.0
38-39	28064.0
40-41	27650.0
42-43	31228.0
44-45	37010.0
46-47	38261.0
48-49	37165.0
50-51	34492.0
52-53	32155.0
54-55	36026.0
56-57	38740.0
58-59	39661.0
60-61	38618.0
62-63	37658.0
64-65	38751.0
66-67	41876.0
68-69	43933.0
70-71	43593.0
72-73	40721.0
74-75	41039.0
76-77	43041.0
78-79	45269.0
80-81	45526.0
82-83	42850.0
84-85	41184.0
86-87	43100.0
88-89	45820.0
90-91	46225.0
92-93	45736.0
94-95	52184.0
96-97	160359.0
98-99	1209544.0
100-101	2627150.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.4314925752171
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.48881387522354	82.71412671372691
2	5.596053830855224	10.009268932492885
3	1.134409941508197	3.0435592278372834
4	0.38575902945148655	1.3799602311285433
5	0.15929767538942216	0.7123114436919227
6	0.0783363831081584	0.4203443798583978
7	0.04570098750301749	0.2860975267189339
8	0.027417022180225382	0.1961556172436311
9	0.01892969011214168	0.15236193966436437
>10	0.06326804969008068	0.8788633117080277
>50	0.0013794320516501391	0.08070507947218174
>100	6.043449452470644E-4	0.11124594834479734
>500	2.9737981554774028E-5	0.01499964811212392
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9168545184094708E-5	0.0	1.9168545184094708E-5	0.0
3	0.0	1.9168545184094708E-5	0.0	1.9168545184094708E-5	0.0
4	0.0	1.9168545184094708E-5	0.0	1.9168545184094708E-5	0.0
5	0.0	1.9168545184094708E-5	0.0	3.8337090368189415E-5	0.0
6	1.9168545184094708E-5	1.9168545184094708E-5	0.0	3.8337090368189415E-5	0.0
7	1.9168545184094708E-5	1.9168545184094708E-5	0.0	3.8337090368189415E-5	0.0
8	1.9168545184094708E-5	3.8337090368189415E-5	0.0	3.8337090368189415E-5	0.0
9	1.9168545184094708E-5	1.3417981628866294E-4	0.0	3.8337090368189415E-5	0.0
10-11	1.9168545184094708E-5	1.3417981628866294E-4	0.0	3.8337090368189415E-5	0.0
12-13	1.9168545184094708E-5	1.5334836147275766E-4	0.0	9.584272592047353E-5	0.0
14-15	1.9168545184094708E-5	1.9168545184094707E-4	0.0	1.1501127110456825E-4	0.0
16-17	3.8337090368189415E-5	2.1085399702504179E-4	0.0	1.1501127110456825E-4	0.0
18-19	4.792136296023677E-5	2.4919108739323117E-4	0.0	1.1501127110456825E-4	1.9168545184094708E-5
20-21	5.7505635552284126E-5	2.875281777614206E-4	0.0	1.1501127110456825E-4	3.8337090368189415E-5
22-23	5.7505635552284126E-5	3.2586526812961004E-4	0.0	1.1501127110456825E-4	3.8337090368189415E-5
24-25	5.7505635552284126E-5	3.929551762739415E-4	0.0	1.1501127110456825E-4	3.8337090368189415E-5
26-27	5.7505635552284126E-5	4.2170799405008357E-4	0.0	1.437640888807103E-4	3.8337090368189415E-5
28-29	5.7505635552284126E-5	4.312922666421309E-4	0.0	3.3544954072165737E-4	3.8337090368189415E-5
30-31	5.7505635552284126E-5	4.7921362960236767E-4	0.0	0.001437640888807103	3.8337090368189415E-5
32-33	5.7505635552284126E-5	4.7921362960236767E-4	0.0	0.0036324393123859473	3.8337090368189415E-5
34-35	5.7505635552284126E-5	5.271349925626044E-4	0.0	0.006919844811458189	5.7505635552284126E-5
36-37	5.7505635552284126E-5	5.750563555228412E-4	0.0	0.010878149391973747	5.7505635552284126E-5
38-39	5.7505635552284126E-5	5.94224900706936E-4	0.0	0.01713667939458067	6.708990814433148E-5
40-41	6.708990814433148E-5	6.708990814433147E-4	0.0	0.02373065893790925	7.667418073637883E-5
42-43	8.625845332842618E-5	6.996518992194568E-4	0.0	0.03003711030347641	7.667418073637883E-5
44-45	9.584272592047353E-5	7.85910352547883E-4	0.0	0.03744575301712901	7.667418073637883E-5
46-47	9.584272592047353E-5	8.434159881001671E-4	0.0	0.04516109245372713	9.584272592047353E-5
48-49	9.584272592047353E-5	8.625845332842618E-4	0.0	0.05347065679103219	9.584272592047353E-5
50-51	9.584272592047353E-5	0.0010159328947570196	0.0	0.0613872659520633	9.584272592047353E-5
52-53	9.584272592047353E-5	0.0010159328947570196	0.0	0.06916011102421371	9.584272592047353E-5
54-55	9.584272592047353E-5	0.0010446857125331614	0.0	0.07672210209933908	9.584272592047353E-5
56-57	9.584272592047353E-5	0.0010734385303093035	0.0	0.08213721611384582	1.0542699851252089E-4
58-59	9.584272592047353E-5	0.001083022802901351	0.0	0.0882040606646118	1.1501127110456825E-4
60-61	1.1501127110456825E-4	0.0010926070754933984	0.0	0.09397379276502431	1.1501127110456825E-4
62-63	1.3417981628866294E-4	0.0010926070754933984	0.0	0.09962851359433224	1.1501127110456825E-4
64-65	1.3417981628866294E-4	0.001245955436966156	0.0	0.10475609943107758	1.1501127110456825E-4
66-67	1.3417981628866294E-4	0.001245955436966156	0.0	0.10988368526782291	1.1501127110456825E-4
68-69	1.3417981628866294E-4	0.0012651239821502508	0.0	0.11541381055343423	1.1501127110456825E-4
70-71	1.3417981628866294E-4	0.0012842925273343455	0.0	0.12064682338869209	1.1501127110456825E-4
72-73	1.3417981628866294E-4	0.001332213890294582	0.0	0.12436552115440647	1.3417981628866294E-4
74-75	1.3417981628866294E-4	0.0013609667080707244	0.0	0.1275474996549662	1.3417981628866294E-4
76-77	1.437640888807103E-4	0.0014280566162150558	0.0	0.13084448942663046	1.3417981628866294E-4
78-79	1.5334836147275766E-4	0.0014951465243593872	0.0	0.13303928785020933	1.3417981628866294E-4
80-81	1.7251690665685238E-4	0.0015143150695434819	0.0	0.13389228811090154	1.5334836147275766E-4
82-83	1.7251690665685238E-4	0.0015334836147275765	0.0	0.13425649046939933	1.7251690665685238E-4
84-85	1.7251690665685238E-4	0.0015526521599116714	0.0	0.13440025455828003	1.8210117924889974E-4
86-87	1.7251690665685238E-4	0.0015526521599116714	0.0	0.13452485010197665	1.9168545184094707E-4
88	1.7251690665685238E-4	0.00162932634064805	0.0	0.13452485010197665	2.1085399702504179E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5880	0.0	49.3763	1
GTATCAA	10960	0.0	38.11146	1
TCAACGC	12250	0.0	32.86902	4
ATCAACG	12480	0.0	32.2289	3
TATCAAC	12845	0.0	31.65056	2
CAACGCA	12745	0.0	31.558783	5
AACGCAG	13145	0.0	30.631077	6
ACGCAGA	14940	0.0	27.03694	7
CGCAGAG	15275	0.0	26.443985	8
GCAGAGT	18595	0.0	21.676487	9
GTACATG	14455	0.0	21.271996	1
TACATGG	13995	0.0	21.266388	2
ACATGGG	14075	0.0	20.19865	3
GAGTACT	11380	0.0	19.782187	12-13
CAGAGTA	18245	0.0	18.966488	10-11
GATCAAC	2260	0.0	18.785986	1
AGAGTAC	16640	0.0	18.669914	10-11
CATGGGG	10110	0.0	18.15306	4
GTGGTAT	3230	0.0	17.924145	1
GTACTTT	12880	0.0	17.428589	14-15
>>END_MODULE