FastQCFastQC Report
Fri 27 May 2016
SRR2138675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5384119
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64450.047.708441
GTATCAA116900.038.0133441
TCAACGC130000.033.6222724
ATCAACG132150.033.042993
TATCAAC134900.032.561782
CAACGCA134050.032.5428355
AACGCAG138700.031.4518176
ACGCAGA159550.027.2882397
CGCAGAG164050.026.5397058
GTACATG165350.022.7482091
GCAGAGT197300.022.218399
TACATGG166100.022.1063182
ACATGGG171050.020.89143
GAGTACT112200.020.39029112-13
CAGAGTA191650.019.22434210-11
CATGGGG117250.019.2029364
AGAGTAC177200.018.06065810-11
GTACTTT127400.017.77361114-15
GATCAAC21750.016.4703521
AGTACTT124150.016.10915212-13
Produced by FastQC (version 0.11.5)