##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138675_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5384119
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.819107267131354	28.0	18.0	33.0	18.0	33.0
2	30.738476434120418	32.0	29.0	33.0	27.0	33.0
3	28.876356930446747	31.0	28.0	33.0	18.0	33.0
4	32.00496515771661	33.0	32.0	33.0	31.0	33.0
5	32.75443113348721	33.0	33.0	33.0	32.0	34.0
6	37.08133828394209	38.0	38.0	38.0	36.0	38.0
7	37.036483034643176	38.0	38.0	38.0	35.0	38.0
8	37.348321424545034	38.0	38.0	38.0	36.0	38.0
9	37.457986348370085	38.0	38.0	38.0	37.0	38.0
10-11	37.424340546707825	38.0	38.0	38.0	37.0	38.0
12-13	37.54458965338619	38.0	38.0	38.0	37.5	38.0
14-15	37.52190460500594	38.0	38.0	38.0	37.0	38.0
16-17	37.57326036070154	38.0	38.0	38.0	38.0	38.0
18-19	37.561521578553524	38.0	38.0	38.0	38.0	38.0
20-21	37.565472721707245	38.0	38.0	38.0	38.0	38.0
22-23	37.57599325004492	38.0	38.0	38.0	38.0	38.0
24-25	37.58234301558478	38.0	38.0	38.0	38.0	38.0
26-27	37.54265317309496	38.0	38.0	38.0	38.0	38.0
28-29	37.51640204930054	38.0	38.0	38.0	38.0	38.0
30-31	37.52544226616203	38.0	38.0	38.0	38.0	38.0
32-33	37.5153049603462	38.0	38.0	38.0	38.0	38.0
34-35	37.411765456039106	38.0	38.0	38.0	37.0	38.0
36-37	37.45479667021891	38.0	38.0	38.0	37.0	38.0
38-39	37.4708474226355	38.0	38.0	38.0	37.0	38.0
40-41	37.4678533075179	38.0	38.0	38.0	38.0	38.0
42-43	37.4371293629121	38.0	38.0	38.0	37.0	38.0
44-45	37.41159587838686	38.0	38.0	38.0	37.0	38.0
46-47	37.39084266220912	38.0	38.0	38.0	37.0	38.0
48-49	37.34090401374256	38.0	38.0	38.0	37.0	38.0
50-51	37.32094859519833	38.0	38.0	38.0	37.0	38.0
52-53	37.307681255029806	38.0	38.0	38.0	37.0	38.0
54-55	37.28652209925444	38.0	38.0	38.0	37.0	38.0
56-57	37.24645964742397	38.0	38.0	38.0	37.0	38.0
58-59	37.241836258427966	38.0	38.0	38.0	37.0	38.0
60-61	37.24613189242914	38.0	38.0	38.0	37.0	38.0
62-63	37.25432475611395	38.0	38.0	38.0	37.0	38.0
64-65	37.2614828855118	38.0	38.0	38.0	37.0	38.0
66-67	37.2609134009604	38.0	38.0	38.0	37.0	38.0
68-69	37.254097687848216	38.0	38.0	38.0	37.0	38.0
70-71	37.25174032112837	38.0	38.0	38.0	37.0	38.0
72-73	37.24430176600208	38.0	38.0	38.0	37.0	38.0
74-75	37.24319812102001	38.0	38.0	38.0	37.0	38.0
76-77	37.232864955953985	38.0	38.0	38.0	37.0	38.0
78-79	37.22095599963929	38.0	38.0	38.0	36.0	38.0
80-81	37.21335538393277	38.0	38.0	38.0	36.0	38.0
82-83	37.170648831484854	38.0	38.0	38.0	36.0	38.0
84-85	37.161567959164316	38.0	38.0	38.0	36.0	38.0
86-87	37.15325391109962	38.0	38.0	38.0	36.0	38.0
88-89	37.14199871294378	38.0	38.0	38.0	36.0	38.0
90-91	37.134888150611815	38.0	38.0	38.0	36.0	38.0
92-93	37.12152659471488	38.0	38.0	38.0	36.0	38.0
94-95	37.108787462882525	38.0	38.0	38.0	36.0	38.0
96-97	37.09958942783807	38.0	38.0	38.0	36.0	38.0
98-99	37.11166231295965	38.0	38.0	38.0	36.0	38.0
100	35.797984470042145	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	14.0
21	56.0
22	190.0
23	650.0
24	1626.0
25	3535.0
26	6393.0
27	10625.0
28	16860.0
29	24633.0
30	33209.0
31	42899.0
32	55668.0
33	74677.0
34	114746.0
35	224064.0
36	799488.0
37	3974782.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.141101450395134	18.316181347403354	11.425081800755146	23.117635401446364
2	14.588244821901034	20.584062518342623	42.66583030502063	22.16186235473572
3	19.028813964787886	26.507952368994676	30.03607317605909	24.427160490158347
4	12.548552511562244	17.80584715902453	40.11506803620053	29.530532293212687
5	13.432819742654276	38.097133440029836	35.49453494620011	12.975511871115774
6	29.705732729904373	38.33427530112169	19.182191181138457	12.777800787835483
7	26.53314311960787	33.07666491026666	23.690505354729343	16.699686615396132
8	23.963771974579316	37.795895670210854	21.450566007177777	16.789766348032057
9	26.31351573024296	17.251754651039477	21.614826863967902	34.81990275474966
10-11	24.327926258687818	27.06382418367796	29.21137144257027	19.396878115063952
12-13	25.165686345342664	24.786209220115676	29.290140132489643	20.757964302052017
14-15	22.388667486732743	26.003632163405005	26.90713188174333	24.700568468118924
16-17	21.828455128870665	29.03224278661003	27.64145814756323	21.497843936956073
18-19	21.831222526842367	28.302801256807285	29.241144187192	20.62483202915835
20-21	22.684356136190292	27.446181740410296	29.11582957835147	20.753632545047942
22-23	22.457148254145824	27.380057413338797	28.92495747306271	21.237836859452663
24-25	22.151433133101513	27.521954560234068	29.012488027465988	21.314124279198428
26-27	22.07233406685513	27.719711946250175	28.93481563437994	21.27313835251475
28-29	22.08073848582681	27.768838713681582	28.94914204186269	21.20128075862892
30-31	22.346444824811133	27.6662581744172	28.834683019251834	21.15261398151983
32-33	22.103698828634545	27.754980238733946	28.82754169216735	21.313779240464157
34-35	22.237639203417924	27.84289683188196	28.753764620736348	21.165699343963766
36-37	22.245914962545	27.724029943008716	28.795479533858327	21.23457556058796
38-39	22.075300373563074	27.874304839793385	28.911890013201486	21.138504773442055
40-41	22.347236529985018	27.708327607595695	28.73717869134752	21.207257171071774
42-43	22.258074510624525	27.772808326687894	28.582006350071904	21.387110812615678
44-45	22.263139686418402	27.9302570072824	28.47189637999633	21.33470692630287
46-47	22.37560978158455	27.91099167529248	28.243649070008704	21.469749473114266
48-49	22.294125300370986	27.990772400375537	28.22070164946318	21.4944006497903
50-51	22.25493009797292	28.159665979965087	28.11784467439337	21.46755924766862
52-53	22.457314876042876	28.0637465501471	28.00517329447588	21.473765279334145
54-55	22.478384242165877	27.943004147186745	28.011755048196413	21.566856562450962
56-57	22.444057680995087	28.087186861606973	28.01044522715393	21.458310230244006
58-59	22.442008861239096	28.135052764208446	28.018374779625155	21.4045635949273
60-61	22.58395863152861	28.09223473932193	27.989067184495248	21.334739444654208
62-63	22.547969742883787	28.20463365439271	27.95795803844589	21.28943856427761
64-65	22.568184717014102	28.140635296081722	27.90909348898077	21.38208649792341
66-67	22.898447481285626	28.21855187545448	27.640404608690204	21.242596034569686
68-69	22.513194239044232	28.18309792483215	27.85405869587111	21.449649140252507
70-71	22.406360398699615	28.127811370537152	27.963482098754405	21.502346132008828
72-73	22.656325222728167	28.26944350999036	27.72861522694158	21.345616040339888
74-75	22.330883268967234	28.222699352906353	27.901268856837596	21.545148521288812
76-77	22.340732977624782	27.95275577537938	27.96050134434651	21.746009902649327
78-79	22.24931669378217	28.097357942006823	27.923519584845547	21.729805779365467
80-81	22.28878239761185	28.105981611979118	27.93100878331627	21.674227207092763
82-83	22.32361018529041	27.9314912164635	28.087902092439553	21.656996505806536
84-85	22.42762957460889	27.912696199304342	27.974283889221773	21.68539033686499
86-87	22.258066465734345	27.949107217989255	28.025781820599327	21.767044495677073
88-89	22.30071604542491	27.900881789281552	28.01608223076377	21.782319934529767
90-91	22.349640827370507	27.969794160921037	28.017534026098428	21.66303098561003
92-93	22.42458515291724	27.965749810947766	27.92660983167884	21.68305520445616
94-95	22.272486161687603	28.02264349272965	27.968113430323292	21.736756915259456
96-97	22.301176936499882	28.101568011088695	28.03254829647414	21.56470675593728
98-99	23.39340696099921	30.039654846270007	28.2384078640674	18.328530328663383
100	29.61979318008283	40.893541419002624	29.486665400914546	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	572.0
1	600.5
2	905.5
3	1317.0
4	1845.0
5	2796.0
6	3525.5
7	4024.0
8	4698.0
9	5190.5
10	5268.5
11	5284.0
12	5847.0
13	7371.5
14	10119.0
15	13263.5
16	16134.5
17	18661.0
18	19718.5
19	18939.5
20	17797.0
21	17279.0
22	18333.0
23	21829.0
24	27682.5
25	36185.5
26	47027.0
27	59000.5
28	71363.5
29	84575.5
30	97065.0
31	109371.0
32	123255.5
33	137554.0
34	150627.5
35	162234.5
36	174219.5
37	185551.0
38	194505.0
39	200977.0
40	207029.0
41	213126.0
42	216875.5
43	219639.5
44	223401.0
45	226867.0
46	226498.5
47	224671.5
48	223097.5
49	218549.0
50	213240.5
51	206044.5
52	196354.5
53	185140.5
54	172317.0
55	158513.5
56	143439.0
57	128279.5
58	112852.0
59	96118.0
60	79950.5
61	64760.5
62	50304.0
63	38333.0
64	28769.0
65	21435.0
66	15806.5
67	11729.5
68	8831.0
69	6589.0
70	4677.0
71	3210.0
72	2278.0
73	1544.0
74	1001.0
75	658.5
76	426.0
77	245.0
78	155.5
79	112.0
80	75.0
81	51.5
82	32.5
83	17.0
84	9.0
85	9.0
86	8.0
87	4.0
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008970826982093078
3	0.001170107867229532
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	4.643325720755592E-5
22-23	0.0
24-25	1.2073519187515312E-4
26-27	2.600695202980545E-4
28-29	0.0015144252723573069
30-31	0.002352696334136443
32-33	0.0020403238804537903
34-35	4.762660295036533E-4
36-37	9.371715506581052E-6
38-39	5.653333624794089E-5
40-41	1.2320192710558965E-4
42-43	0.0019066058650056316
44-45	1.7278674103663982E-4
46-47	4.354008779423303E-4
48-49	0.0011215935132492865
50-51	8.452648362161779E-4
52-53	0.0
54-55	0.00290109219641092
56-57	0.05060596014810785
58-59	0.06644351267676549
60-61	0.04296460475068109
62-63	0.0069063165377212694
64-65	0.0
66-67	0.0
68-69	7.412934554165995E-5
70-71	0.001358693617156941
72-73	0.007131720390312573
74-75	0.011649339183599476
76-77	0.01271301600220594
78-79	0.004008153474868655
80-81	0.007272180375289536
82-83	0.044332431546798945
84-85	0.03778052036370048
86-87	0.008716225497818503
88-89	0.0021504873439119293
90-91	0.0025088066843170927
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	25.0
20-21	105.0
22-23	231.0
24-25	400.0
26-27	1182.0
28-29	3086.0
30-31	9626.0
32-33	11734.0
34-35	16459.0
36-37	26828.0
38-39	31002.0
40-41	30670.0
42-43	34162.0
44-45	40481.0
46-47	41327.0
48-49	40240.0
50-51	37088.0
52-53	34873.0
54-55	38637.0
56-57	41890.0
58-59	42171.0
60-61	41303.0
62-63	40282.0
64-65	42175.0
66-67	45008.0
68-69	47431.0
70-71	47446.0
72-73	43494.0
74-75	44487.0
76-77	47276.0
78-79	48959.0
80-81	49325.0
82-83	46180.0
84-85	44805.0
86-87	46668.0
88-89	49343.0
90-91	49798.0
92-93	49156.0
94-95	54807.0
96-97	163332.0
98-99	1219744.0
100-101	2680883.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.101866785263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.79280737075928	79.08223012667919
2	6.597549876678073	11.49317820935084
3	1.5380999806829843	4.019141388595947
4	0.5221538737289358	1.8192230860378724
5	0.24060482223729737	1.0478564587202481
6	0.10446879645912986	0.5459656315439931
7	0.0648059733037746	0.3951304877516281
8	0.03852444026870178	0.26844405314090275
9	0.024830045169812867	0.1946468957987793
>10	0.07451512260599367	0.960009304859561
>50	8.764121111883473E-4	0.04841036799771113
>100	7.632859948520222E-4	0.12576398952335835
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.857314074967511E-5	0.0	1.857314074967511E-5	0.0
3	0.0	1.857314074967511E-5	0.0	1.857314074967511E-5	0.0
4	0.0	5.571942224902533E-5	0.0	1.857314074967511E-5	0.0
5	1.857314074967511E-5	7.429256299870043E-5	0.0	7.429256299870043E-5	0.0
6	5.571942224902533E-5	7.429256299870043E-5	0.0	7.429256299870043E-5	0.0
7	5.571942224902533E-5	7.429256299870043E-5	0.0	7.429256299870043E-5	0.0
8	5.571942224902533E-5	9.286570374837555E-5	0.0	7.429256299870043E-5	0.0
9	5.571942224902533E-5	2.043045482464262E-4	0.0	9.286570374837555E-5	0.0
10-11	5.571942224902533E-5	2.3216425937093888E-4	0.0	1.1143884449805066E-4	0.0
12-13	5.571942224902533E-5	2.4145082974577641E-4	0.0	1.392985556225633E-4	0.0
14-15	5.571942224902533E-5	2.6002397049545154E-4	0.0	1.4858512599740087E-4	0.0
16-17	7.429256299870043E-5	2.9717025199480174E-4	0.0	1.67158266747076E-4	0.0
18-19	7.429256299870043E-5	3.0645682236963927E-4	0.0	1.67158266747076E-4	0.0
20-21	9.286570374837555E-5	3.528896742438271E-4	0.0	2.043045482464262E-4	0.0
22-23	1.0215227412321311E-4	4.0860909649285244E-4	0.0	2.043045482464262E-4	9.286570374837554E-6
24-25	1.392985556225633E-4	5.293345113657406E-4	0.0	2.2287768899610132E-4	1.857314074967511E-5
26-27	1.4858512599740087E-4	6.036270743644411E-4	0.0	2.878836816199642E-4	1.857314074967511E-5
28-29	1.4858512599740087E-4	6.129136447392787E-4	0.0	6.500599262386289E-4	1.857314074967511E-5
30-31	1.4858512599740087E-4	6.964927781128166E-4	0.0	0.0014951378303488462	1.857314074967511E-5
32-33	1.67158266747076E-4	7.429256299870044E-4	0.0	0.003445317609064733	1.857314074967511E-5
34-35	1.857314074967511E-4	8.636510448598926E-4	0.0	0.005878399047272172	3.714628149935022E-5
36-37	2.043045482464262E-4	0.001021522741232131	0.0	0.009370149508211092	3.714628149935022E-5
38-39	2.043045482464262E-4	0.0010586690227314813	0.0	0.014589202058869797	3.714628149935022E-5
40-41	2.043045482464262E-4	0.001170107867229532	0.0	0.020644045943263883	3.714628149935022E-5
42-43	2.043045482464262E-4	0.0012072541487288821	0.0	0.02605811647179418	3.714628149935022E-5
44-45	2.1359111862126375E-4	0.0013279795636017703	0.0	0.032196539489561804	3.714628149935022E-5
46-47	2.2287768899610132E-4	0.0014301318377249834	0.0	0.03873428503344744	3.714628149935022E-5
48-49	2.2287768899610132E-4	0.0014301318377249834	0.0	0.045559914258953046	3.714628149935022E-5
50-51	2.2287768899610132E-4	0.0016994423785952725	0.0	0.05219981207696189	3.714628149935022E-5
52-53	2.2287768899610132E-4	0.0017644483712191354	0.0	0.05879327704309656	4.643285187418777E-5
54-55	2.2287768899610132E-4	0.0017830215119688105	0.0	0.06596250937247115	5.571942224902533E-5
56-57	2.4145082974577641E-4	0.0018201677934681607	0.0	0.07283457144985095	6.500599262386289E-5
58-59	2.6002397049545154E-4	0.0018480275045926736	0.0	0.0799945172088507	7.429256299870043E-5
60-61	2.6002397049545154E-4	0.0018944603564668612	0.0	0.08704302412335241	7.429256299870043E-5
62-63	2.6002397049545154E-4	0.0019130334972165364	0.0	0.09374792793398512	9.286570374837555E-5
64-65	2.6002397049545154E-4	0.002024472341714587	0.0	0.10011851521112367	9.286570374837555E-5
66-67	2.6002397049545154E-4	0.002024472341714587	0.0	0.10636837707338935	9.286570374837555E-5
68-69	2.878836816199642E-4	0.0020801917639636123	0.0	0.11272039120977824	9.286570374837555E-5
70-71	2.9717025199480174E-4	0.0021266246158378	0.0	0.11809731545680918	9.286570374837555E-5
72-73	2.9717025199480174E-4	0.0021359111862126376	0.0	0.12270345436272861	9.286570374837555E-5
74-75	2.9717025199480174E-4	0.002154484326962313	0.0	0.12578659572717468	9.286570374837555E-5
76-77	3.1574339274447686E-4	0.0022009171788365004	0.0	0.12928763275848842	9.286570374837555E-5
78-79	3.1574339274447686E-4	0.0022566366010855258	0.0	0.1314792633669501	9.286570374837555E-5
80-81	3.1574339274447686E-4	0.0022659231714603633	0.0	0.1321014635820642	9.286570374837555E-5
82-83	3.1574339274447686E-4	0.002275209741835201	0.0	0.13263079809342998	9.286570374837555E-5
84-85	3.1574339274447686E-4	0.0022844963122100386	0.0	0.13285367578242607	1.1143884449805066E-4
86-87	3.3431653349415193E-4	0.0022844963122100386	0.0	0.13298368776767377	1.1143884449805066E-4
88	3.5288967424382706E-4	0.0023216425937093886	0.0	0.13300226090842346	1.1143884449805066E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6445	0.0	47.70844	1
GTATCAA	11690	0.0	38.013344	1
TCAACGC	13000	0.0	33.622272	4
ATCAACG	13215	0.0	33.04299	3
TATCAAC	13490	0.0	32.56178	2
CAACGCA	13405	0.0	32.542835	5
AACGCAG	13870	0.0	31.451817	6
ACGCAGA	15955	0.0	27.288239	7
CGCAGAG	16405	0.0	26.539705	8
GTACATG	16535	0.0	22.748209	1
GCAGAGT	19730	0.0	22.21839	9
TACATGG	16610	0.0	22.106318	2
ACATGGG	17105	0.0	20.8914	3
GAGTACT	11220	0.0	20.390291	12-13
CAGAGTA	19165	0.0	19.224342	10-11
CATGGGG	11725	0.0	19.202936	4
AGAGTAC	17720	0.0	18.060658	10-11
GTACTTT	12740	0.0	17.773611	14-15
GATCAAC	2175	0.0	16.470352	1
AGTACTT	12415	0.0	16.109152	12-13
>>END_MODULE