FastQCFastQC Report
Fri 27 May 2016
SRR2138674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5542783
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59150.047.056821
GTATCAA104500.035.145781
TCAACGC110750.032.117424
ATCAACG112750.031.4724433
CAACGCA113600.031.2740235
AACGCAG118300.030.1760796
TATCAAC119050.030.0961512
ACGCAGA138900.025.7007227
CGCAGAG140200.025.4624128
GTACATG142700.022.6513521
ACATGGG136100.022.0519963
TACATGG141200.021.862452
GCAGAGT173500.020.7232389
GAGTACT106150.019.13086712-13
CAGAGTA167400.018.78400810-11
CATGGGG103100.017.6649324
AGAGTAC154800.016.86070610-11
GTACTTT125450.016.06852314-15
GTACTAG14100.015.1616761
ATGGGGG67050.015.0478445
Produced by FastQC (version 0.11.5)