##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138674_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5542783
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.550577210040515	27.0	18.0	33.0	18.0	33.0
2	30.35364599335749	32.0	27.0	33.0	27.0	33.0
3	28.6054180724737	30.0	27.0	31.0	18.0	33.0
4	31.947663294774483	33.0	32.0	33.0	31.0	33.0
5	32.713409310810114	33.0	33.0	33.0	32.0	34.0
6	37.0734196161026	38.0	37.0	38.0	36.0	38.0
7	37.108150003346694	38.0	38.0	38.0	36.0	38.0
8	37.37732453173794	38.0	38.0	38.0	37.0	38.0
9	37.48437743278061	38.0	38.0	38.0	37.0	38.0
10-11	37.44899818376436	38.0	38.0	38.0	37.0	38.0
12-13	37.550492595506626	38.0	38.0	38.0	37.5	38.0
14-15	37.522929095365996	38.0	38.0	38.0	37.0	38.0
16-17	37.561005816031404	38.0	38.0	38.0	38.0	38.0
18-19	37.554108558823245	38.0	38.0	38.0	38.0	38.0
20-21	37.558045093411394	38.0	38.0	38.0	38.0	38.0
22-23	37.563077740036285	38.0	38.0	38.0	38.0	38.0
24-25	37.56817005261238	38.0	38.0	38.0	38.0	38.0
26-27	37.5359471692465	38.0	38.0	38.0	38.0	38.0
28-29	37.511809396684626	38.0	38.0	38.0	38.0	38.0
30-31	37.51475386471357	38.0	38.0	38.0	38.0	38.0
32-33	37.50321567776399	38.0	38.0	38.0	38.0	38.0
34-35	37.402340900229454	38.0	38.0	38.0	37.0	38.0
36-37	37.44378637920529	38.0	38.0	38.0	37.0	38.0
38-39	37.448909171587424	38.0	38.0	38.0	37.0	38.0
40-41	37.44834722334339	38.0	38.0	38.0	37.0	38.0
42-43	37.41665332707294	38.0	38.0	38.0	37.0	38.0
44-45	37.39208660827339	38.0	38.0	38.0	37.0	38.0
46-47	37.37033173762126	38.0	38.0	38.0	37.0	38.0
48-49	37.3197795741996	38.0	38.0	38.0	37.0	38.0
50-51	37.292101259924664	38.0	38.0	38.0	37.0	38.0
52-53	37.271691129251984	38.0	38.0	38.0	37.0	38.0
54-55	37.240844276499985	38.0	38.0	38.0	37.0	38.0
56-57	37.193833957696896	38.0	38.0	38.0	37.0	38.0
58-59	37.183726120491926	38.0	38.0	38.0	37.0	38.0
60-61	37.184965938014415	38.0	38.0	38.0	36.5	38.0
62-63	37.18862047257828	38.0	38.0	38.0	36.0	38.0
64-65	37.19539928606444	38.0	38.0	38.0	36.5	38.0
66-67	37.190001653227384	38.0	38.0	38.0	36.0	38.0
68-69	37.177655916546186	38.0	38.0	38.0	36.0	38.0
70-71	37.17265357389278	38.0	38.0	38.0	36.0	38.0
72-73	37.16207435155211	38.0	38.0	38.0	36.0	38.0
74-75	37.15859417015899	38.0	38.0	38.0	36.0	38.0
76-77	37.145684573941175	38.0	38.0	38.0	36.0	38.0
78-79	37.13181769604999	38.0	38.0	38.0	36.0	38.0
80-81	37.118872641866986	38.0	38.0	38.0	36.0	38.0
82-83	37.07169990078491	38.0	38.0	38.0	36.0	38.0
84-85	37.062404887893685	38.0	38.0	38.0	36.0	38.0
86-87	37.04862221376301	38.0	38.0	38.0	36.0	38.0
88-89	37.03235782166787	38.0	38.0	38.0	36.0	38.0
90-91	37.020235841514705	38.0	38.0	38.0	36.0	38.0
92-93	37.002238853257694	38.0	38.0	38.0	36.0	38.0
94-95	36.986935635175215	38.0	38.0	38.0	36.0	38.0
96-97	36.97624329255255	38.0	38.0	38.0	35.5	38.0
98-99	36.99141925500335	38.0	38.0	38.0	35.0	38.0
100	35.60272685297762	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	6.0
20	11.0
21	54.0
22	266.0
23	775.0
24	1758.0
25	3835.0
26	7076.0
27	11867.0
28	18882.0
29	27364.0
30	36037.0
31	46215.0
32	59952.0
33	82028.0
34	124911.0
35	239475.0
36	866304.0
37	4015965.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.70484429933483	17.519791050813282	11.438459705169768	23.33690494468212
2	14.512765496010546	20.345874195875332	42.75222369091012	22.389136617204
3	19.168377765986563	26.12326807010188	30.00222092603053	24.706133237881023
4	12.511007557034073	17.53357474034253	40.31086910672851	29.644548595894875
5	13.34041040394329	37.89289604157334	35.6871629288031	13.079530625680277
6	29.4858557515241	38.33648187201267	19.320005852655605	12.857656523807625
7	26.12904023123402	32.80079700035163	24.096902224027172	16.973260544387177
8	23.526358509795532	38.02986694590064	21.733703087420164	16.710071456883664
9	25.932352033265598	17.07133402119477	21.829773960120754	35.16653998541888
10-11	24.169861962844298	27.01024918348779	29.400555641453042	19.419333212214877
12-13	24.89749282986543	24.58008007890621	29.55531544352359	20.967111647704773
14-15	22.0690671094286	25.8652377334635	27.041776667064184	25.023918490043716
16-17	21.680291290494324	28.832853460075924	27.80305489137857	21.68380035805118
18-19	21.683809378790404	28.24561235754674	29.31765504801469	20.752923215648167
20-21	22.419223789548717	27.486170414198835	29.14458408766139	20.950021708591056
22-23	22.238282630285287	27.38909996553071	28.937926507801237	21.43469089638276
24-25	21.973740273895686	27.436606264960332	29.10263677124867	21.487016689895317
26-27	21.91965159743155	27.683406217942874	28.971194715136512	21.42574746948906
28-29	21.944835408319705	27.749665279171072	28.98166173865676	21.323837573852465
30-31	22.135165047361667	27.576550072010253	28.931703063773107	21.35658181685497
32-33	21.95165249426105	27.684231546353455	28.9147295028072	21.449386456578296
34-35	22.06951101640949	27.738875990145566	28.828528090452142	21.363084902992803
36-37	22.11262504515867	27.625814107341597	28.836321313164127	21.425239534335606
38-39	21.91381831327911	27.79617179255625	28.93487890625572	21.35513098790892
40-41	22.1788857632418	27.62083300212269	28.808048344977227	21.39223288965828
42-43	22.03609544719034	27.748028048493428	28.661295492195837	21.554581012120398
44-45	22.08251490679695	27.834783144097376	28.564620742377254	21.518081206728418
46-47	22.11919033913681	27.835816923071288	28.35032542634668	21.694667311445222
48-49	22.14374351567427	27.904365272920035	28.29952061524749	21.652370596158203
50-51	22.0557397216446	28.038489420290574	28.265958797726142	21.639812060338684
52-53	22.21354461925841	27.95598224828042	28.175262661761423	21.655210470699746
54-55	22.22192893401015	27.863292240754166	28.140788010635724	21.77399081459995
56-57	22.233126972897548	27.939612467737497	28.177481414690874	21.649779144674085
58-59	22.216821778913893	28.02477731693194	28.18769851996279	21.57070238419138
60-61	22.353541007288673	27.922661665456218	28.198874251796198	21.524923075458915
62-63	22.25590191209461	28.023402798942776	28.161811068864385	21.55888422009823
64-65	22.249770327050854	27.951905643229836	28.189864851863337	21.60845917785597
66-67	22.39399120902709	28.019965883349673	27.980430084446027	21.605612823177207
68-69	22.253755290937704	27.94094259798149	28.143315129655296	21.661986981425517
70-71	22.192891751496777	27.86471628650059	28.25618567538495	21.686206286617683
72-73	22.41185467246854	27.906666249526662	28.132968189342776	21.54851088866202
74-75	22.091120400961618	27.999668801328003	28.178078851264445	21.731131946445934
76-77	22.23659958478878	27.798783372270936	28.103793419836382	21.860823623103897
78-79	22.142892540259158	27.893994630675817	28.109273934645927	21.85383889441909
80-81	22.14776064025175	27.901261887677155	28.14174071956119	21.809236752509907
82-83	22.157729826562072	27.87152850501488	28.174473917138908	21.79626775128414
84-85	22.303303787340038	27.834314621612506	28.107259931434964	21.75512165961249
86-87	22.109772394147694	27.836905130260313	28.17051327770089	21.8828091978911
88-89	22.177061967486434	27.845118762876293	28.1254386741988	21.85238059543847
90-91	22.227324220120945	27.928023765685722	28.042089223335893	21.802562790857436
92-93	22.264892037284092	27.941684594691058	28.010766228518186	21.782657139506664
94-95	22.147659531676993	27.95744528204468	28.039762586496973	21.855132599781356
96-97	22.205760750136253	28.0231876032802	28.147742489020004	21.623309157563543
98-99	23.274955224454892	29.99605797017615	28.35299463209938	18.37599217326958
100	29.57219549333611	40.84761205868389	29.580192447979993	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	504.0
1	540.5
2	888.5
3	1339.5
4	1840.5
5	2824.0
6	3693.5
7	4394.5
8	5159.5
9	5463.5
10	5217.0
11	5041.0
12	5615.5
13	6938.0
14	9330.5
15	12344.5
16	15099.5
17	17311.5
18	18089.0
19	17478.5
20	16641.5
21	16189.5
22	17268.0
23	21016.0
24	27410.0
25	36258.0
26	47714.0
27	60970.5
28	74033.5
29	87827.5
30	101465.0
31	114719.5
32	128583.5
33	142703.0
34	155962.0
35	168285.0
36	181291.5
37	192134.0
38	200538.5
39	206873.0
40	213274.0
41	219068.5
42	222589.5
43	225539.5
44	230284.5
45	234540.5
46	234618.5
47	234459.5
48	233485.5
49	228934.5
50	222801.5
51	214641.0
52	202909.0
53	190038.5
54	175687.5
55	160737.0
56	146167.5
57	130626.5
58	114537.5
59	97027.5
60	79380.0
61	63713.5
62	49937.5
63	37818.0
64	27765.0
65	20330.0
66	14898.0
67	11182.5
68	8261.5
69	5850.5
70	4219.5
71	2945.5
72	2036.0
73	1407.0
74	938.0
75	624.0
76	406.5
77	250.0
78	146.5
79	86.5
80	59.0
81	40.0
82	27.0
83	16.0
84	8.5
85	6.5
86	6.0
87	4.0
88	2.0
89	0.5
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007595462423840154
3	9.020739220712773E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.118726817652133E-5
22-23	9.021011017180334E-6
24-25	1.1727830611153492E-4
26-27	2.435997108200766E-4
28-29	0.002174870999015713
30-31	0.0031068303484581184
32-33	0.0022891030371420995
34-35	3.990221056432789E-4
36-37	0.0
38-39	4.573853602472296E-5
40-41	1.748053105853356E-4
42-43	0.0024519253767152026
44-45	2.5150047980702645E-4
46-47	6.008383760728133E-4
48-49	0.0011732559337655393
50-51	9.438078719868576E-4
52-53	0.0
54-55	0.0032679618495426496
56-57	0.05279720940729724
58-59	0.06875344897250098
60-61	0.04506671121810326
62-63	0.007743708237057391
64-65	0.0
66-67	0.0
68-69	1.744233947327623E-4
70-71	0.001264158969026551
72-73	0.007763223767601304
74-75	0.01289354074791829
76-77	0.013541371448775338
78-79	0.0042013411973822416
80-81	0.0070128388309162095
82-83	0.04507296371741992
84-85	0.037917862348819206
86-87	0.00809995592770166
88-89	0.002339540991143134
90-91	0.002734095747803332
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	22.0
20-21	101.0
22-23	222.0
24-25	397.0
26-27	982.0
28-29	2604.0
30-31	9559.0
32-33	11889.0
34-35	16308.0
36-37	26896.0
38-39	31753.0
40-41	30086.0
42-43	34305.0
44-45	41112.0
46-47	41768.0
48-49	40523.0
50-51	37352.0
52-53	35920.0
54-55	39475.0
56-57	42455.0
58-59	42441.0
60-61	42370.0
62-63	40899.0
64-65	42572.0
66-67	45794.0
68-69	47550.0
70-71	47379.0
72-73	44125.0
74-75	44932.0
76-77	47284.0
78-79	49756.0
80-81	49431.0
82-83	46653.0
84-85	45534.0
86-87	47443.0
88-89	49770.0
90-91	50428.0
92-93	50176.0
94-95	57045.0
96-97	170003.0
98-99	1273917.0
100-101	2763552.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.82227233125587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.14463263338845	80.04528748661713
2	6.539441221169645	11.486171756396022
3	1.3736701144795205	3.619164926613837
4	0.45676335036466986	1.6045598138665154
5	0.19699612896200885	0.8650323842952372
6	0.10295267413203116	0.542492267091257
7	0.05411953946885162	0.3327030653572947
8	0.03744226646920246	0.26306119380461995
9	0.022704030730908355	0.17945276128803325
>10	0.06988044639315429	0.9336164351419984
>50	9.80453400703148E-4	0.056111325107307784
>100	4.1714104090384167E-4	0.07234658442067998
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.8041478441425545E-5	0.0
2	0.0	0.0	0.0	3.608295688285109E-5	0.0
3	0.0	0.0	0.0	3.608295688285109E-5	0.0
4	0.0	3.608295688285109E-5	0.0	3.608295688285109E-5	0.0
5	0.0	3.608295688285109E-5	0.0	3.608295688285109E-5	0.0
6	1.8041478441425545E-5	3.608295688285109E-5	0.0	3.608295688285109E-5	0.0
7	1.8041478441425545E-5	3.608295688285109E-5	0.0	3.608295688285109E-5	0.0
8	1.8041478441425545E-5	3.608295688285109E-5	0.0	3.608295688285109E-5	0.0
9	1.8041478441425545E-5	3.608295688285109E-5	0.0	9.020739220712772E-5	0.0
10-11	1.8041478441425545E-5	3.608295688285109E-5	0.0	9.922813142784049E-5	0.0
12-13	1.8041478441425545E-5	3.608295688285109E-5	0.0	1.1726960986926604E-4	0.0
14-15	1.8041478441425545E-5	5.412443532427663E-5	0.0	1.262903490899788E-4	0.0
16-17	1.8041478441425545E-5	5.412443532427663E-5	0.0	1.262903490899788E-4	0.0
18-19	1.8041478441425545E-5	6.31451745449894E-5	0.0	1.4433182753140436E-4	9.020739220712773E-6
20-21	1.8041478441425545E-5	7.216591376570218E-5	0.0	1.4433182753140436E-4	1.8041478441425545E-5
22-23	1.8041478441425545E-5	9.020739220712772E-5	0.0	1.8041478441425544E-4	3.608295688285109E-5
24-25	1.8041478441425545E-5	1.1726960986926604E-4	0.0	1.9845626285568097E-4	6.31451745449894E-5
26-27	1.8041478441425545E-5	1.4433182753140436E-4	0.0	2.7062217662138314E-4	7.216591376570218E-5
28-29	1.8041478441425545E-5	1.4433182753140436E-4	0.0	5.051613963599152E-4	7.216591376570218E-5
30-31	1.8041478441425545E-5	2.525806981799576E-4	0.0	0.0010734679672648197	7.216591376570218E-5
32-33	1.8041478441425545E-5	2.7062217662138314E-4	0.0	0.002616014374006704	8.118665298641496E-5
34-35	1.8041478441425545E-5	3.15725872724947E-4	0.0	0.005015531006716301	1.0824887064855326E-4
36-37	1.8041478441425545E-5	3.698503080492236E-4	0.0	0.00819985195162791	1.262903490899788E-4
38-39	1.8041478441425545E-5	3.9691252571136195E-4	0.0	0.014171581315739763	1.4433182753140433E-4
40-41	1.8041478441425545E-5	4.3299548259421306E-4	0.0	0.0206935757723151	1.623733059728299E-4
42-43	1.8041478441425545E-5	4.510369610356386E-4	0.0	0.02639468295980557	1.7139404519354265E-4
44-45	2.706221766213832E-5	4.780991786977769E-4	0.0	0.032393474541579564	1.894355236349682E-4
46-47	3.608295688285109E-5	4.871199179184897E-4	0.0	0.039276298566983406	1.9845626285568097E-4
48-49	3.608295688285109E-5	4.961406571392025E-4	0.0	0.04672742916329216	2.4355995895924482E-4
50-51	3.608295688285109E-5	5.592858316841919E-4	0.0	0.05410639384583521	2.525806981799576E-4
52-53	3.608295688285109E-5	5.592858316841919E-4	0.0	0.061656752573571796	2.525806981799576E-4
54-55	3.608295688285109E-5	5.592858316841919E-4	0.0	0.06901767577767343	2.525806981799576E-4
56-57	3.608295688285109E-5	6.134102670084685E-4	0.0	0.07470976222594318	2.525806981799576E-4
58-59	3.608295688285109E-5	6.134102670084685E-4	0.0	0.08022143388979869	2.525806981799576E-4
60-61	3.608295688285109E-5	6.134102670084685E-4	0.0	0.08618414251468982	2.886636550628087E-4
62-63	3.608295688285109E-5	6.314517454498941E-4	0.0	0.09169581417854533	2.886636550628087E-4
64-65	3.608295688285109E-5	7.216591376570218E-4	0.0	0.09712629918941441	2.886636550628087E-4
66-67	3.608295688285109E-5	7.397006160984473E-4	0.0	0.10266503307093205	2.886636550628087E-4
68-69	3.608295688285109E-5	7.397006160984473E-4	0.0	0.10825789138777397	2.886636550628087E-4
70-71	3.608295688285109E-5	7.397006160984473E-4	0.0	0.11304790391397246	2.886636550628087E-4
72-73	3.608295688285109E-5	7.487213553191601E-4	0.0	0.1170260499103068	3.0670513350423425E-4
74-75	3.608295688285109E-5	7.667628337605856E-4	0.0	0.12060728338092977	3.0670513350423425E-4
76-77	3.608295688285109E-5	8.028457906434367E-4	0.0	0.12381866654350351	3.0670513350423425E-4
78-79	3.608295688285109E-5	8.208872690848622E-4	0.0	0.1262001416977717	3.0670513350423425E-4
80-81	3.608295688285109E-5	8.389287475262878E-4	0.0	0.12693984231387012	3.0670513350423425E-4
82-83	3.608295688285109E-5	8.479494867470005E-4	0.0	0.12766150145152716	3.247466119456598E-4
84-85	3.608295688285109E-5	8.659909651884261E-4	0.0	0.12791408214970712	3.247466119456598E-4
86-87	3.608295688285109E-5	8.659909651884261E-4	0.0	0.12801331028113494	3.247466119456598E-4
88	3.608295688285109E-5	9.201154005127028E-4	0.0	0.12802233102035565	3.247466119456598E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5915	0.0	47.05682	1
GTATCAA	10450	0.0	35.14578	1
TCAACGC	11075	0.0	32.11742	4
ATCAACG	11275	0.0	31.472443	3
CAACGCA	11360	0.0	31.274023	5
AACGCAG	11830	0.0	30.176079	6
TATCAAC	11905	0.0	30.096151	2
ACGCAGA	13890	0.0	25.700722	7
CGCAGAG	14020	0.0	25.462412	8
GTACATG	14270	0.0	22.651352	1
ACATGGG	13610	0.0	22.051996	3
TACATGG	14120	0.0	21.86245	2
GCAGAGT	17350	0.0	20.723238	9
GAGTACT	10615	0.0	19.130867	12-13
CAGAGTA	16740	0.0	18.784008	10-11
CATGGGG	10310	0.0	17.664932	4
AGAGTAC	15480	0.0	16.860706	10-11
GTACTTT	12545	0.0	16.068523	14-15
GTACTAG	1410	0.0	15.161676	1
ATGGGGG	6705	0.0	15.047844	5
>>END_MODULE