FastQCFastQC Report
Fri 27 May 2016
SRR2138673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9135054
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119250.049.53861
GTATCAA219050.038.0203631
TCAACGC260500.031.1292384
ATCAACG261900.031.0797143
CAACGCA265950.030.477835
TATCAAC274800.029.8920422
AACGCAG273950.029.7959446
ACGCAGA313150.025.9960677
CGCAGAG314950.025.833858
GCAGAGT371200.021.7783839
GTACATG278000.020.855531
GAGTACT214600.020.3661212-13
TACATGG276250.020.3526172
ACATGGG280900.019.3599623
AGAGTAC336350.019.06609710-11
GTGGTAT57250.018.8449131
CAGAGTA370150.018.77674910-11
CATGGGG201350.018.3400174
GTACTTT246850.017.70565414-15
AGTACTT241100.016.79965612-13
Produced by FastQC (version 0.11.5)