##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138673_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9135054
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64439673810357	33.0	33.0	33.0	33.0	33.0
2	32.64716563251843	33.0	33.0	33.0	33.0	33.0
3	32.647317793633185	33.0	33.0	33.0	33.0	33.0
4	36.56161780762325	37.0	37.0	37.0	37.0	37.0
5	36.58356064452383	37.0	37.0	37.0	37.0	37.0
6	36.56968661597403	37.0	37.0	37.0	37.0	37.0
7	36.53699332264484	37.0	37.0	37.0	37.0	37.0
8	36.53679190073753	37.0	37.0	37.0	37.0	37.0
9	36.57245222633605	37.0	37.0	37.0	37.0	37.0
10-11	36.500046086208144	37.0	37.0	37.0	37.0	37.0
12-13	36.49759032623124	37.0	37.0	37.0	37.0	37.0
14-15	38.60604102613953	40.0	37.0	40.0	37.0	40.0
16-17	38.55157594032832	40.0	37.0	40.0	37.0	40.0
18-19	38.57020002290079	40.0	37.0	40.0	37.0	40.0
20-21	38.556300187649576	40.0	37.0	40.0	37.0	40.0
22-23	38.56299493559493	40.0	37.0	40.0	37.0	40.0
24-25	38.550949352795335	40.0	37.0	40.0	37.0	40.0
26-27	38.455064132903956	40.0	37.0	40.0	37.0	40.0
28-29	38.39638913615881	40.0	37.0	40.0	37.0	40.0
30-31	38.34411979775351	40.0	37.0	40.0	37.0	40.0
32-33	38.28322511707266	40.0	37.0	40.0	37.0	40.0
34-35	38.24868529965918	40.0	37.0	40.0	37.0	40.0
36-37	38.19425438106599	40.0	37.0	40.0	37.0	40.0
38-39	38.148461905953006	40.0	37.0	40.0	37.0	40.0
40-41	38.11801287160638	40.0	37.0	40.0	35.0	40.0
42-43	38.06432809235595	40.0	37.0	40.0	33.0	40.0
44-45	38.0032677262971	40.0	37.0	40.0	33.0	40.0
46-47	37.96809758155171	40.0	37.0	40.0	33.0	40.0
48-49	37.94940470425762	40.0	37.0	40.0	33.0	40.0
50-51	37.87933679250986	40.0	37.0	40.0	33.0	40.0
52-53	37.77285891270628	40.0	37.0	40.0	33.0	40.0
54-55	37.58417810699121	40.0	37.0	40.0	33.0	40.0
56-57	37.509646971848056	40.0	37.0	40.0	33.0	40.0
58-59	37.395963186751146	40.0	37.0	40.0	33.0	40.0
60-61	37.26419561642089	37.0	37.0	40.0	33.0	40.0
62-63	37.10610551884758	37.0	37.0	40.0	33.0	40.0
64-65	36.92346157647558	37.0	37.0	40.0	33.0	40.0
66-67	36.72029721089328	37.0	37.0	40.0	33.0	40.0
68-69	36.51857517668418	37.0	37.0	40.0	33.0	40.0
70-71	36.25516126225314	37.0	37.0	40.0	33.0	40.0
72-73	36.03415186406406	37.0	37.0	37.0	33.0	40.0
74-75	35.839095971304104	37.0	37.0	37.0	33.0	40.0
76-77	34.200758616586675	35.0	33.0	37.0	30.0	37.0
78-79	35.27828999651738	37.0	33.0	37.0	33.0	37.0
80-81	35.325256835848144	37.0	33.0	37.0	33.0	37.0
82-83	35.19595587076695	37.0	33.0	37.0	33.0	37.0
84-85	35.03167307743297	37.0	33.0	37.0	33.0	37.0
86-87	34.94863756620941	37.0	33.0	37.0	33.0	37.0
88-89	34.84893976804531	37.0	33.0	37.0	33.0	37.0
90-91	34.74186063597726	37.0	33.0	37.0	33.0	37.0
92-93	34.71490741902208	37.0	33.0	37.0	33.0	37.0
94-95	34.744944002183686	37.0	33.0	37.0	33.0	37.0
96-97	34.73535575429304	37.0	33.0	37.0	33.0	37.0
98-99	34.71136772100401	37.0	33.0	37.0	33.0	37.0
100	34.694285188810404	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	5.0
20	65.0
21	343.0
22	1209.0
23	3581.0
24	8146.0
25	16173.0
26	28062.0
27	44369.0
28	64978.0
29	91268.0
30	123264.0
31	163093.0
32	214881.0
33	288685.0
34	397534.0
35	580747.0
36	1026561.0
37	2586414.0
38	3413252.0
39	82421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.25886940569809	20.483863587451154	14.51840350369029	23.738863503160463
2	14.267731750682591	21.45079821093559	41.855231507115334	22.42623853126648
3	17.481921836477376	26.997519664361043	30.970895191205216	24.54966330795636
4	12.119249650850449	18.713135138555284	39.73680943757968	29.43080577301459
5	12.99730685773724	38.14603613727954	35.9112381820622	12.945418822921026
6	28.898689486334167	38.615676071182584	19.457881100517962	13.027753341965292
7	25.712393161551102	33.18270477656727	23.849141997409102	17.255760064472526
8	24.090103900863642	36.60592482540333	21.71092803611232	17.59304323762071
9	25.642169165064598	16.814678928006337	21.469998973186147	36.07315293374292
10-11	23.80971304349492	26.880015457756777	29.500548602575545	19.809722896172755
12-13	24.815401991395234	24.614237301865536	29.360044728806976	21.210315977932257
14-15	21.97397027358893	25.901385725803056	27.083825619782825	25.040818380825193
16-17	21.378510734583507	28.95683484739116	27.759715487177196	21.904938930848136
18-19	21.359397547075258	28.14117464439729	29.510526155619882	20.98890165290758
20-21	22.311554367282213	27.28594741244576	29.338751281770868	21.06374693850116
22-23	21.986896223966273	27.303542164408967	29.0874224791965	21.622139132428256
24-25	21.747586656738257	27.42928731649281	29.24677542476555	21.57635060200338
26-27	21.686168079908057	27.623304348549915	29.102999246565766	21.58752832497626
28-29	21.607467878128002	27.67330821739532	29.107345844844772	21.611878059631902
30-31	21.871277124735546	27.50159948290239	29.053232216409075	21.57389117595299
32-33	21.7285670129797	27.614543731166542	28.977839446026493	21.679049809827262
34-35	21.851270803100498	27.699953185206528	28.940558957088452	21.508217054604522
36-37	21.855466009968826	27.650313469553467	28.79910673106837	21.695113789409337
38-39	21.727362574593773	27.770044051110602	28.886850672747645	21.615742701547976
40-41	21.981146473135173	27.653275715167624	28.701706872720894	21.663870938976313
42-43	21.882428450765964	27.736710312248857	28.534600543134463	21.846260693850713
44-45	21.990471838695075	27.8635736774271	28.331696190291815	21.814258293586008
46-47	22.03996244546557	27.843069724363307	28.026448603863933	22.090519226307194
48-49	22.021099765473807	27.949138285922526	28.009868978304542	22.019892970299125
50-51	22.040217845715283	28.057543510892796	27.867931234403255	22.03430740898867
52-53	22.09508716123907	28.013625268292575	27.790899037695265	22.100388532773085
54-55	22.19899257297148	27.895152130810185	27.792373618389565	22.11348167782877
56-57	22.154324757169963	27.98214753116204	27.77421779845722	22.089309913210784
58-59	22.056004206147662	28.07656952910645	27.85000742807534	22.01741883667055
60-61	22.16896158744743	28.066360583854365	27.758235196348668	22.006442632349536
62-63	22.101570009198618	28.13283802980812	27.69820132236135	22.067390638631913
64-65	22.0864849259337	28.049261226252543	27.698963240054162	22.165290607759598
66-67	22.15308416810407	28.08115640977989	27.617108848366733	22.148650573749308
68-69	22.099798805135798	28.06021087127365	27.654272088687808	22.18571823490274
70-71	22.10616134885375	27.86448167147912	27.677911133348204	22.351445846318924
72-73	22.169813984216045	27.897328210000698	27.656099605902007	22.276758199881254
74-75	22.118554631295446	27.970035501496483	27.58818875701337	22.323221110194698
76-77	22.147884344281426	27.97156391269112	27.546424142421543	22.334127600605903
78-79	22.196004740765627	27.95032086146853	27.453705906785736	22.399968490980108
80-81	22.11347052622747	27.994164077180606	27.54787288270313	22.344492513888795
82-83	22.192327673143694	27.936156859303797	27.609996763624938	22.26151870392757
84-85	22.294141769753786	27.915845389226813	27.514761303006207	22.275251538013194
86-87	22.127202851551424	27.92970143667228	27.600634164675185	22.342461547101113
88-89	22.2142626043686	27.86178170945744	27.577245792514994	22.34670989365897
90-91	22.2329784798836	27.92243654341517	27.582052186084937	22.262532790616294
92-93	22.220608035623492	27.895999384553466	27.62093843884213	22.262454140980914
94-95	22.093136522957522	27.95379205345469	27.671398173617867	22.28167324996992
96-97	22.110180365466768	28.057428019594923	27.756019988292273	22.076371626646036
98-99	23.271375995764185	29.907189740706585	27.946301987294635	18.875132276234595
100	22.180624567484834	31.023759499747506	21.71062475451842	25.08499117824924
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1844.0
1	1637.5
2	2271.0
3	3293.5
4	4468.0
5	7452.0
6	10004.5
7	11318.0
8	11753.5
9	11115.0
10	10040.5
11	9183.5
12	10389.5
13	14307.0
14	22888.0
15	34775.0
16	44955.0
17	51616.0
18	53356.5
19	50220.0
20	44887.5
21	39632.0
22	36226.5
23	36891.5
24	41899.5
25	51598.5
26	65620.0
27	82652.5
28	102090.5
29	125008.5
30	148509.5
31	171659.5
32	197260.0
33	221211.5
34	243478.0
35	265031.0
36	287379.5
37	307572.5
38	319537.5
39	329225.0
40	340952.0
41	349466.5
42	354211.0
43	359525.5
44	363928.5
45	368277.5
46	370769.0
47	370008.5
48	365365.0
49	355361.5
50	343864.0
51	329195.5
52	311884.5
53	292220.5
54	270975.0
55	247915.0
56	224450.5
57	201323.0
58	177585.5
59	153273.0
60	127204.0
61	102970.0
62	81599.5
63	62785.5
64	46890.5
65	35109.5
66	26512.5
67	20277.0
68	15635.0
69	11450.0
70	8320.5
71	5922.5
72	4158.0
73	2976.5
74	2032.0
75	1405.0
76	918.5
77	526.5
78	331.5
79	225.5
80	152.5
81	103.0
82	77.0
83	64.0
84	49.0
85	32.5
86	20.0
87	13.5
88	7.5
89	5.5
90	5.0
91	3.0
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0946842788230918E-4
7	0.0
8	0.0
9	0.0
10-11	0.005270904802533187
12-13	1.6420264182346377E-5
14-15	0.004187167366498326
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	138.0
20-21	514.0
22-23	1115.0
24-25	2359.0
26-27	3211.0
28-29	5181.0
30-31	10426.0
32-33	13568.0
34-35	18767.0
36-37	24910.0
38-39	30184.0
40-41	35259.0
42-43	44871.0
44-45	54902.0
46-47	52831.0
48-49	45626.0
50-51	38861.0
52-53	37996.0
54-55	41951.0
56-57	44930.0
58-59	47522.0
60-61	47928.0
62-63	49957.0
64-65	54204.0
66-67	58913.0
68-69	59245.0
70-71	59875.0
72-73	59490.0
74-75	62681.0
76-77	62245.0
78-79	68216.0
80-81	70929.0
82-83	71061.0
84-85	72342.0
86-87	77088.0
88-89	82913.0
90-91	87201.0
92-93	92417.0
94-95	113996.0
96-97	319036.0
98-99	594235.0
100-101	6415960.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.6956977135289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.96025554116363	71.04278749782131
2	8.793343537406857	14.36756671046407
3	2.3899314941707877	5.857413627114573
4	0.8804430202686649	2.8771362735142096
5	0.40207297831741484	1.6423816247698892
6	0.20172169122924705	0.9887876585355827
7	0.12703229055688134	0.7264594126434506
8	0.07478768519732419	0.4887865698060126
9	0.04530816729888059	0.333133410564301
>10	0.12274801899579386	1.443619419048337
>50	0.0018533695749115022	0.09778596980464416
>100	3.8884175682991846E-4	0.05690239297909891
>500	9.06915066617732E-5	0.050863388880008374
>1k	2.2672556041053712E-5	0.02637604405445506
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0946842788230919E-5
2	0.0	2.1893685576461837E-5	0.0	0.0	1.0946842788230919E-5
3	0.0	2.1893685576461837E-5	0.0	0.0	1.0946842788230919E-5
4	0.0	6.568105672938551E-5	0.0	1.0946842788230919E-5	1.0946842788230919E-5
5	0.0	6.568105672938551E-5	0.0	2.1893685576461837E-5	2.1893685576461837E-5
6	0.0	6.568105672938551E-5	0.0	2.1893685576461837E-5	3.2840528364692754E-5
7	1.0946842788230919E-5	6.568105672938551E-5	0.0	3.2840528364692754E-5	4.3787371152923675E-5
8	2.1893685576461837E-5	6.568105672938551E-5	0.0	4.3787371152923675E-5	4.3787371152923675E-5
9	3.2840528364692754E-5	1.9704317018815653E-4	0.0	6.568105672938551E-5	6.568105672938551E-5
10-11	3.2840528364692754E-5	1.9704317018815653E-4	0.0	9.30481636999628E-5	6.568105672938551E-5
12-13	3.2840528364692754E-5	1.9704317018815653E-4	0.0	1.2588869206465555E-4	6.568105672938551E-5
14-15	3.831394975880822E-5	2.4083054134108018E-4	0.0	1.5325579903523287E-4	6.568105672938551E-5
16-17	4.3787371152923675E-5	2.517773841293111E-4	0.0	1.9156974879404106E-4	6.568105672938551E-5
18-19	4.3787371152923675E-5	2.6819764831165747E-4	0.0	2.0799001297638745E-4	9.30481636999628E-5
20-21	4.3787371152923675E-5	2.846179124940039E-4	0.0	2.1893685576461835E-4	1.3136211345877102E-4
22-23	4.3787371152923675E-5	3.2840528364692754E-4	0.0	2.3535711994696474E-4	1.5325579903523287E-4
24-25	4.3787371152923675E-5	3.995597617704285E-4	0.0	2.6819764831165747E-4	1.6420264182346377E-4
26-27	5.473421394115459E-5	4.269268687410058E-4	0.0	3.174584408586966E-4	1.6420264182346377E-4
28-29	5.473421394115459E-5	4.378737115292367E-4	0.0	4.2145344734689034E-4	1.6967606321757924E-4
30-31	5.473421394115459E-5	4.5976739710569856E-4	0.0	8.155397877232034E-4	1.8062290600581016E-4
32-33	5.473421394115459E-5	4.5976739710569856E-4	0.0	0.0018281227456345634	1.860963273999256E-4
34-35	5.473421394115459E-5	5.035547682586222E-4	0.0	0.0031636375657987355	1.860963273999256E-4
36-37	6.568105672938551E-5	5.582889821997768E-4	0.0	0.0052927984881096495	1.860963273999256E-4
38-39	6.568105672938551E-5	5.692358249880078E-4	0.0	0.01233161840094213	1.9704317018815653E-4
40-41	6.568105672938551E-5	6.513371458997397E-4	0.0	0.020054615988039044	1.9704317018815653E-4
42-43	6.568105672938551E-5	7.060713598408942E-4	0.0	0.024624922852125447	1.9704317018815653E-4
44-45	6.568105672938551E-5	8.429068946937807E-4	0.0	0.030558111643346604	1.9704317018815653E-4
46-47	8.210132091173189E-5	9.30481636999628E-4	0.0	0.03698938178143227	2.134634343705029E-4
48-49	8.757474230584735E-5	9.359550583937434E-4	0.0	0.04409935617238825	2.2988369855284928E-4
50-51	8.757474230584735E-5	0.0011275248071877845	0.0	0.05065651500253857	2.408305413410802E-4
52-53	8.757474230584735E-5	0.0011384716499760156	0.0	0.06928256800671348	2.572508055234266E-4
54-55	8.757474230584735E-5	0.0011494184927642464	0.0	0.09326162713433331	2.7367106970577296E-4
56-57	9.852158509407826E-5	0.0011603653355524773	0.0	0.12024559460732252	2.7914449109988845E-4
58-59	1.0946842788230918E-4	0.0011603653355524773	0.0	0.14676432126181194	2.846179124940039E-4
60-61	1.0946842788230918E-4	0.0011767855997348235	0.0	0.15753601456543115	2.846179124940039E-4
62-63	1.0946842788230918E-4	0.001182259021128939	0.0	0.17117030725817273	3.065115980704657E-4
64-65	1.0946842788230918E-4	0.0013026742917994793	0.0	0.17655068048858824	3.065115980704657E-4
66-67	1.0946842788230918E-4	0.0013026742917994793	0.0	0.18065574653417485	3.119850194645812E-4
68-69	1.0946842788230918E-4	0.001335514820164172	0.0	0.18462945046630266	3.448255478292739E-4
70-71	1.0946842788230918E-4	0.001335514820164172	0.0	0.1885374733417011	3.6124581201162027E-4
72-73	1.0946842788230918E-4	0.0013409882415582875	0.0	0.19157522221543516	3.831394975880821E-4
74-75	1.204152706705401E-4	0.001395722455499442	0.0	0.1942298315915812	3.831394975880821E-4
76-77	1.204152706705401E-4	0.001466876933622943	0.0	0.19697201571003303	3.831394975880821E-4
78-79	1.204152706705401E-4	0.001477823776411174	0.0	0.1987016168705735	3.831394975880821E-4
80-81	1.2588869206465555E-4	0.001477823776411174	0.0	0.19915043742489097	3.995597617704285E-4
82-83	1.3136211345877102E-4	0.001477823776411174	0.0	0.1995499971866614	4.159800259527749E-4
84-85	1.3136211345877102E-4	0.001477823776411174	0.0	0.19974704035684956	4.2145344734689034E-4
86-87	1.3136211345877102E-4	0.0015106643047758668	0.0	0.19985103536333776	4.269268687410058E-4
88	1.3136211345877102E-4	0.0015653985187170213	0.0	0.19985650878473188	4.269268687410058E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11925	0.0	49.5386	1
GTATCAA	21905	0.0	38.020363	1
TCAACGC	26050	0.0	31.129238	4
ATCAACG	26190	0.0	31.079714	3
CAACGCA	26595	0.0	30.47783	5
TATCAAC	27480	0.0	29.892042	2
AACGCAG	27395	0.0	29.795944	6
ACGCAGA	31315	0.0	25.996067	7
CGCAGAG	31495	0.0	25.83385	8
GCAGAGT	37120	0.0	21.778383	9
GTACATG	27800	0.0	20.85553	1
GAGTACT	21460	0.0	20.36612	12-13
TACATGG	27625	0.0	20.352617	2
ACATGGG	28090	0.0	19.359962	3
AGAGTAC	33635	0.0	19.066097	10-11
GTGGTAT	5725	0.0	18.844913	1
CAGAGTA	37015	0.0	18.776749	10-11
CATGGGG	20135	0.0	18.340017	4
GTACTTT	24685	0.0	17.705654	14-15
AGTACTT	24110	0.0	16.799656	12-13
>>END_MODULE