FastQCFastQC Report
Fri 27 May 2016
SRR2138672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8171254
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104950.052.717541
GTATCAA192400.038.9233321
ATCAACG231150.031.4880493
TCAACGC231200.031.3848174
CAACGCA235800.030.7579065
AACGCAG243350.029.8220316
TATCAAC247550.029.7817212
ACGCAGA277850.026.0385327
CGCAGAG281750.025.6943118
GTACATG247800.022.2731111
TACATGG241300.022.2052652
GCAGAGT335500.021.525679
ACATGGG242700.021.320983
GAGTACT203350.020.73743612-13
AGAGTAC303850.019.89974410-11
CATGGGG174150.019.6121624
CAGAGTA327750.019.38437710-11
GTGGTAT53450.018.4244291
GTACTTT230350.018.23883414-15
AGTACTT220200.018.09343512-13
Produced by FastQC (version 0.11.5)