##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138672_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8171254
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65329776800476	33.0	33.0	33.0	33.0	33.0
2	32.65847310584153	33.0	33.0	33.0	33.0	33.0
3	32.65825588092109	33.0	33.0	33.0	33.0	33.0
4	36.57476820571237	37.0	37.0	37.0	37.0	37.0
5	36.59732814571668	37.0	37.0	37.0	37.0	37.0
6	36.57937092642084	37.0	37.0	37.0	37.0	37.0
7	36.5474837766639	37.0	37.0	37.0	37.0	37.0
8	36.54606771494314	37.0	37.0	37.0	37.0	37.0
9	36.58241856145948	37.0	37.0	37.0	37.0	37.0
10-11	36.507877615846965	37.0	37.0	37.0	37.0	37.0
12-13	36.508258157193495	37.0	37.0	37.0	37.0	37.0
14-15	38.63163120372956	40.0	37.0	40.0	37.0	40.0
16-17	38.57900592736439	40.0	37.0	40.0	37.0	40.0
18-19	38.59663553476614	40.0	37.0	40.0	37.0	40.0
20-21	38.58155369978172	40.0	37.0	40.0	37.0	40.0
22-23	38.58790099628997	40.0	37.0	40.0	37.0	40.0
24-25	38.57618572767299	40.0	37.0	40.0	37.0	40.0
26-27	38.481814098536255	40.0	37.0	40.0	37.0	40.0
28-29	38.422268667175274	40.0	37.0	40.0	37.0	40.0
30-31	38.367703579795986	40.0	37.0	40.0	37.0	40.0
32-33	38.305685351486076	40.0	37.0	40.0	37.0	40.0
34-35	38.27256598565634	40.0	37.0	40.0	37.0	40.0
36-37	38.21942379874241	40.0	37.0	40.0	37.0	40.0
38-39	38.17104289825445	40.0	37.0	40.0	37.0	40.0
40-41	38.140376110230136	40.0	37.0	40.0	37.0	40.0
42-43	38.08519932373767	40.0	37.0	40.0	35.0	40.0
44-45	38.02184359496127	40.0	37.0	40.0	33.0	40.0
46-47	37.98189292695551	40.0	37.0	40.0	33.0	40.0
48-49	37.96108074484049	40.0	37.0	40.0	33.0	40.0
50-51	37.88792854557093	40.0	37.0	40.0	33.0	40.0
52-53	37.785366305126814	40.0	37.0	40.0	33.0	40.0
54-55	37.59806855699974	40.0	37.0	40.0	33.0	40.0
56-57	37.519420015777555	40.0	37.0	40.0	33.0	40.0
58-59	37.4045451512105	40.0	37.0	40.0	33.0	40.0
60-61	37.2687274898959	37.0	37.0	40.0	33.0	40.0
62-63	37.10948290685886	37.0	37.0	40.0	33.0	40.0
64-65	36.9260451974256	37.0	37.0	40.0	33.0	40.0
66-67	36.72199555107352	37.0	37.0	40.0	33.0	40.0
68-69	36.52115101750641	37.0	37.0	40.0	33.0	40.0
70-71	36.255080329663684	37.0	37.0	40.0	33.0	40.0
72-73	36.03112656141451	37.0	37.0	37.0	33.0	40.0
74-75	35.83736692013465	37.0	37.0	37.0	33.0	40.0
76-77	34.20649550147894	35.0	33.0	37.0	30.0	37.0
78-79	35.28337311550864	37.0	33.0	37.0	33.0	37.0
80-81	35.325028697030305	37.0	33.0	37.0	33.0	37.0
82-83	35.19312063231203	37.0	33.0	37.0	33.0	37.0
84-85	35.026365109403216	37.0	33.0	37.0	33.0	37.0
86-87	34.94073884475691	37.0	33.0	37.0	33.0	37.0
88-89	34.83637955207431	37.0	33.0	37.0	33.0	37.0
90-91	34.72271085776431	37.0	33.0	37.0	33.0	37.0
92-93	34.691489251553975	37.0	33.0	37.0	33.0	37.0
94-95	34.716648551737705	37.0	33.0	37.0	33.0	37.0
96-97	34.70769512980536	37.0	33.0	37.0	33.0	37.0
98-99	34.69931482793092	37.0	33.0	37.0	33.0	37.0
100	34.708641362127544	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	1.0
19	11.0
20	57.0
21	254.0
22	1033.0
23	3192.0
24	7198.0
25	14164.0
26	25220.0
27	39751.0
28	58785.0
29	82129.0
30	110996.0
31	147801.0
32	194159.0
33	260686.0
34	358913.0
35	524019.0
36	926352.0
37	2343366.0
38	3020638.0
39	52527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.357776909150054	20.535330317721122	14.549957693152116	23.55693507997671
2	14.37705889450995	21.340641228384285	41.92870519017033	22.353594686935445
3	17.503494567663665	26.946622880649652	31.01175168462515	24.53813086706153
4	11.987658197872689	18.67463672038588	39.786683904330964	29.55102117741047
5	12.906880633009326	38.272571137795985	36.0167729457437	12.803775283450985
6	29.06529130642457	38.700535206341364	19.336891422000647	12.897282065233421
7	25.824285966389006	33.15057884628235	23.808646751159614	17.21648843616904
8	24.225412647801672	36.72307824478348	21.563556829832972	17.487952277581876
9	25.70933420990218	16.68165008700011	21.326689881381732	36.282325821715986
10-11	23.80450070323488	26.926638380616268	29.503995441334354	19.764865474814496
12-13	24.879230585613332	24.56884980988535	29.383931119013745	21.16798848548757
14-15	21.959995743433822	25.916711434246793	27.07683989246969	25.046452929849693
16-17	21.396611829714267	29.00426911218278	27.71780194325131	21.88131711485165
18-19	21.38711512333358	28.1445112341386	29.44867458532069	21.01969905720713
20-21	22.33323585526177	27.323635825596128	29.30880459513955	21.03432372400256
22-23	21.978231455705615	27.299224466541546	29.176097438285638	21.546446639467206
24-25	21.760559906293842	27.424799253112266	29.267047251029364	21.547593589564528
26-27	21.738788942282262	27.626038999582736	29.136947148395485	21.498224909739513
28-29	21.646212282428806	27.65916742322525	29.198039735018227	21.496580559327715
30-31	21.91137474998427	27.52786025257969	29.093170277601637	21.467594719834405
32-33	21.779794279459587	27.548912182560592	29.047301692570677	21.62399184540915
34-35	21.843179124557086	27.675040353297735	29.05877671408572	21.42300380805946
36-37	21.899954036266458	27.594088205351742	28.89541411823368	21.610543640148123
38-39	21.720317636616358	27.718536008339246	28.982042725063057	21.579103629981343
40-41	21.983655739792034	27.586710239664875	28.87790098943602	21.55173303110707
42-43	21.875054601312215	27.67089792500763	28.675704575332833	21.77834289834732
44-45	21.990196737139673	27.85970596432553	28.418071398709987	21.732025899824812
46-47	22.077457897480272	27.799273823225647	28.10904315549293	22.014225123801157
48-49	22.010854859757075	27.948608293523687	28.079817848492265	21.960718998226973
50-51	21.99040372470854	28.063668404979904	27.970643930206567	21.97528394010499
52-53	22.116852406189036	28.019315857231998	27.883074930298314	21.980756806280656
54-55	22.158879962946997	27.926083871967876	27.87802473724018	22.03701142784494
56-57	22.154418110095513	28.068314137430832	27.811737068600944	21.965530683872707
58-59	22.086521332028042	28.12816059503735	27.915096560755643	21.870221512178965
60-61	22.166446778599784	28.121278278358115	27.807438910995142	21.904836032046965
62-63	22.08368509946943	28.194004361642854	27.76467604332421	21.957634495563504
64-65	22.1074566527769	28.079925097661718	27.774467308384782	22.0381509411766
66-67	22.169269807297873	28.11280330114875	27.653119958492432	22.06480693306094
68-69	22.11553836416173	28.076678933388173	27.75390373783802	22.05387896461208
70-71	22.10740371193849	27.90311091557134	27.81580546236863	22.173679910121542
72-73	22.13341587521089	27.93554628457935	27.77033972539475	22.160698114815006
74-75	22.07242056815115	28.069551767011458	27.69085309149156	22.167174573345825
76-77	22.150604309369978	28.041111180894713	27.61542456841335	22.19285994132196
78-79	22.121103201874377	28.014060350534503	27.623039928025033	22.24179651956609
80-81	22.098988016730598	28.03484061969354	27.654234343972135	22.21193701960373
82-83	22.15801864454938	28.04588074524077	27.717679849544712	22.078420760665143
84-85	22.225443524663493	28.001060260907796	27.671611350783426	22.101884863645285
86-87	22.10794444441017	28.002367544891733	27.704971110756006	22.184716899942096
88-89	22.159369957080834	28.00149920750057	27.665966566788576	22.17316426863002
90-91	22.189694082072062	28.040154671248363	27.67117758632268	22.098973660356897
92-93	22.17840750545653	28.021915404373733	27.7226631308052	22.077013959364542
94-95	22.077527750643817	28.062353254595507	27.729821507858983	22.130297486901696
96-97	22.12359871368802	28.168102740361412	27.823531683680446	21.88476686227012
98-99	22.321583046846214	28.880014392479346	26.914011304929865	21.884391255744575
100	20.421925493081723	28.671496654426743	28.0560206781156	22.850557174375936
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1538.0
1	1311.5
2	1790.0
3	2672.5
4	3728.0
5	6234.0
6	8264.0
7	9396.5
8	9993.0
9	9608.5
10	8819.5
11	8104.5
12	9008.5
13	12456.5
14	20194.0
15	31013.5
16	40204.0
17	45698.5
18	46693.0
19	43922.0
20	39184.0
21	33815.0
22	30376.0
23	30831.0
24	35176.5
25	43492.0
26	55803.0
27	69956.5
28	85835.5
29	105396.0
30	124738.0
31	143948.5
32	166615.5
33	188098.5
34	208053.5
35	227193.5
36	247470.5
37	265819.0
38	277972.0
39	288275.0
40	299399.0
41	308355.0
42	312850.5
43	316088.5
44	316072.5
45	317021.5
46	320393.0
47	320853.0
48	318050.5
49	311799.5
50	302905.5
51	290033.5
52	273411.0
53	255622.0
54	237926.5
55	219297.5
56	199552.5
57	177816.5
58	156049.5
59	134695.5
60	111296.0
61	89548.5
62	70308.5
63	53147.5
64	38890.5
65	28292.0
66	21095.0
67	16051.0
68	12053.5
69	8653.0
70	6231.5
71	4634.5
72	3299.0
73	2272.5
74	1606.0
75	1118.5
76	752.0
77	449.5
78	269.5
79	183.0
80	126.5
81	86.5
82	66.5
83	54.0
84	43.0
85	29.0
86	20.0
87	20.0
88	16.5
89	10.0
90	4.0
91	1.5
92	1.5
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5909430792385108E-4
7	0.0
8	0.0
9	0.0
10-11	0.005409206469410937
12-13	6.119011843225043E-6
14-15	0.004264951254727855
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	101.0
20-21	453.0
22-23	935.0
24-25	1753.0
26-27	2439.0
28-29	3837.0
30-31	6692.0
32-33	8887.0
34-35	12325.0
36-37	15347.0
38-39	18977.0
40-41	22559.0
42-43	30668.0
44-45	37912.0
46-47	36298.0
48-49	29249.0
50-51	24038.0
52-53	23248.0
54-55	26124.0
56-57	28079.0
58-59	28788.0
60-61	29993.0
62-63	30684.0
64-65	33169.0
66-67	36155.0
68-69	36268.0
70-71	36973.0
72-73	36950.0
74-75	39622.0
76-77	39444.0
78-79	43800.0
80-81	46896.0
82-83	46985.0
84-85	48968.0
86-87	53462.0
88-89	59712.0
90-91	64507.0
92-93	70501.0
94-95	90580.0
96-97	283962.0
98-99	35726.0
100-101	6648188.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.04159979464507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.15658394028924	71.50465579097599
2	8.773118938797532	14.395214258552969
3	2.3260526262355254	5.724992359886942
4	0.8522236808374921	2.796711766351554
5	0.3819284002749209	1.5667008482782037
6	0.18575043760159637	0.9143557838034216
7	0.1008428097674931	0.5791313808778537
8	0.06318397255425436	0.41469713517855783
9	0.0380807097006253	0.2811782110639283
>10	0.11887763008995585	1.4585288304288089
>50	0.002338312608416358	0.12609105962086734
>100	8.634718688967314E-4	0.14811513215488145
>500	1.2922452972050644E-4	0.06517977989676299
>1k	2.5844844374634637E-5	0.02444766292916273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2238023686450085E-5
2	0.0	0.0	0.0	0.0	1.2238023686450085E-5
3	0.0	0.0	0.0	0.0	1.2238023686450085E-5
4	0.0	2.447604737290017E-5	0.0	1.2238023686450085E-5	1.2238023686450085E-5
5	0.0	2.447604737290017E-5	0.0	2.447604737290017E-5	1.2238023686450085E-5
6	0.0	2.447604737290017E-5	0.0	2.447604737290017E-5	2.447604737290017E-5
7	0.0	2.447604737290017E-5	0.0	2.447604737290017E-5	2.447604737290017E-5
8	0.0	2.447604737290017E-5	0.0	2.447604737290017E-5	4.895209474580034E-5
9	0.0	9.790418949160068E-5	0.0	6.119011843225042E-5	6.119011843225042E-5
10-11	4.895209474580034E-5	9.790418949160068E-5	0.0	9.178517764837563E-5	6.119011843225042E-5
12-13	7.34281421187005E-5	9.790418949160068E-5	0.0	1.2238023686450084E-4	6.119011843225042E-5
14-15	7.34281421187005E-5	1.8357035529675128E-4	0.0	1.2238023686450084E-4	1.0402320133482573E-4
16-17	8.566616580515059E-5	1.8357035529675128E-4	0.0	1.4685628423740104E-4	1.3461826055095094E-4
18-19	9.790418949160068E-5	2.0804640266965145E-4	0.0	1.7745134345352622E-4	1.590943079238511E-4
20-21	9.790418949160068E-5	2.2028442635610153E-4	0.0	1.9580837898320135E-4	1.590943079238511E-4
22-23	9.790418949160068E-5	2.3864146188577664E-4	0.0	2.019273908264264E-4	1.7133233161030118E-4
24-25	1.346182605509509E-4	2.8147454478835195E-4	0.0	2.0804640266965143E-4	1.9580837898320135E-4
26-27	1.5297529608062607E-4	2.93712568474802E-4	0.0	2.2640343819932656E-4	2.141654145128765E-4
28-29	1.590943079238511E-4	2.9983158031802705E-4	0.0	2.5699849741545175E-4	2.2640343819932656E-4
30-31	1.9580837898320135E-4	3.6714071059350256E-4	0.0	3.2430762769092725E-4	2.3864146188577664E-4
32-33	2.2028442635610153E-4	3.6714071059350256E-4	0.0	5.507110658902538E-4	2.569984974154518E-4
34-35	2.508794855722267E-4	4.160928053393029E-4	0.0	8.811377054244061E-4	2.8147454478835195E-4
36-37	2.8147454478835195E-4	4.650449000851032E-4	0.0	0.0013400635936662844	2.8147454478835195E-4
38-39	2.8147454478835195E-4	4.895209474580033E-4	0.0	0.002215082287247465	2.87593556631577E-4
40-41	2.8147454478835195E-4	5.507110658902538E-4	0.0	0.003077862957142196	2.93712568474802E-4
42-43	2.8147454478835195E-4	5.568300777334789E-4	0.0	0.004026309792842077	2.93712568474802E-4
44-45	2.998315803180271E-4	6.119011843225043E-4	0.0	0.005078779829876786	2.9983158031802705E-4
46-47	3.2430762769092725E-4	6.608532790683046E-4	0.0	0.006186320973500518	3.2430762769092725E-4
48-49	3.304266395341523E-4	6.608532790683046E-4	0.0	0.007869049230387405	3.7937873427995264E-4
50-51	3.304266395341523E-4	7.832335159328054E-4	0.0	0.009264183930642714	3.7937873427995264E-4
52-53	3.6102169875027747E-4	7.832335159328054E-4	0.0	0.010775579855919301	4.0997379349607787E-4
54-55	3.6714071059350256E-4	7.893525277760305E-4	0.0	0.012446070089119736	4.528068763986531E-4
56-57	3.6714071059350256E-4	8.077095633057056E-4	0.0	0.014324606724989825	4.650449000851032E-4
58-59	3.7937873427995264E-4	8.077095633057056E-4	0.0	0.016307166562194737	4.650449000851032E-4
60-61	3.916167579664027E-4	8.077095633057056E-4	0.0	0.018234655292810625	4.650449000851032E-4
62-63	3.916167579664027E-4	8.138285751489306E-4	0.0	0.020321238331350366	4.895209474580033E-4
64-65	3.916167579664027E-4	9.668038712295567E-4	0.0	0.022432297417263006	5.017589711444534E-4
66-67	3.916167579664027E-4	9.668038712295567E-4	0.0	0.0248737831427098	5.507110658902538E-4
68-69	3.916167579664027E-4	9.790418949160067E-4	0.0	0.027339744915529488	5.81306125106379E-4
70-71	4.038547816528528E-4	9.912799186024568E-4	0.0	0.029603779297522753	6.241392080089544E-4
72-73	4.038547816528528E-4	9.912799186024568E-4	0.0	0.03147619692154962	7.220433975005549E-4
74-75	4.038547816528528E-4	9.912799186024568E-4	0.0	0.03306102098894491	7.220433975005549E-4
76-77	4.038547816528528E-4	0.0010341130015050321	0.0	0.034474512724729886	7.281624093437801E-4
78-79	4.038547816528528E-4	0.0010647080607211574	0.0	0.03576562422365037	7.342814211870051E-4
80-81	4.038547816528528E-4	0.0010647080607211574	0.0	0.03627350220663805	7.465194448734552E-4
82-83	4.1609280533930285E-4	0.0010769460844076075	0.0	0.036640642917231556	8.444236343650559E-4
84-85	4.1609280533930285E-4	0.0010891841080940576	0.0	0.03676914216593928	8.444236343650559E-4
86-87	4.1609280533930285E-4	0.0010891841080940576	0.0	0.03683645129621475	8.444236343650559E-4
88	4.1609280533930285E-4	0.001150374226526308	0.0	0.03684868931990121	8.444236343650559E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10495	0.0	52.71754	1
GTATCAA	19240	0.0	38.923332	1
ATCAACG	23115	0.0	31.488049	3
TCAACGC	23120	0.0	31.384817	4
CAACGCA	23580	0.0	30.757906	5
AACGCAG	24335	0.0	29.822031	6
TATCAAC	24755	0.0	29.781721	2
ACGCAGA	27785	0.0	26.038532	7
CGCAGAG	28175	0.0	25.694311	8
GTACATG	24780	0.0	22.273111	1
TACATGG	24130	0.0	22.205265	2
GCAGAGT	33550	0.0	21.52567	9
ACATGGG	24270	0.0	21.32098	3
GAGTACT	20335	0.0	20.737436	12-13
AGAGTAC	30385	0.0	19.899744	10-11
CATGGGG	17415	0.0	19.612162	4
CAGAGTA	32775	0.0	19.384377	10-11
GTGGTAT	5345	0.0	18.424429	1
GTACTTT	23035	0.0	18.238834	14-15
AGTACTT	22020	0.0	18.093435	12-13
>>END_MODULE