FastQCFastQC Report
Fri 27 May 2016
SRR2138671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5945229
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84500.049.090231
GTATCAA149850.037.4206541
TCAACGC176150.031.2059524
ATCAACG177200.031.095913
CAACGCA179450.030.609765
TATCAAC185050.029.991872
AACGCAG184550.029.9555826
ACGCAGA208750.026.4614777
CGCAGAG213100.025.98388
GCAGAGT246450.022.396989
TACATGG180900.021.2681432
GTACATG186150.021.2622361
ACATGGG181000.020.865473
GAGTACT149250.020.8014512-13
GTGGTAT42550.019.6431521
CAGAGTA245900.019.52427910-11
AGAGTAC226600.019.0792210-11
CTTATAC36300.018.7613051
GTACTTT167150.018.5343814-15
CATGGGG125150.018.268744
Produced by FastQC (version 0.11.5)