FastQCFastQC Report
Fri 27 May 2016
SRR2138670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8531745
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106400.049.7926371
GTATCAA194200.037.6248051
ATCAACG232200.030.8589943
TCAACGC233050.030.6897934
CAACGCA237800.029.9872785
AACGCAG245850.029.202956
TATCAAC249750.028.9203322
ACGCAGA279150.025.656047
CGCAGAG284100.025.2408478
GTACATG258600.022.313751
TACATGG255050.022.0530972
CTTATAC53300.021.8676551
GCAGAGT336250.021.1046589
ACATGGG260400.020.921833
GAGTACT193900.020.00496512-13
AGAGTAC306500.019.2558210-11
CATGGGG190250.018.7273754
TATACAC81900.018.6516693
CAGAGTA329450.018.63154210-11
GTACTTT219000.017.73237214-15
Produced by FastQC (version 0.11.5)