##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138670_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8531745
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.645827201820964	33.0	33.0	33.0	33.0	33.0
2	32.65201878396506	33.0	33.0	33.0	33.0	33.0
3	32.6517462723042	33.0	33.0	33.0	33.0	33.0
4	36.564930972503284	37.0	37.0	37.0	37.0	37.0
5	36.58694417144441	37.0	37.0	37.0	37.0	37.0
6	36.57103171742709	37.0	37.0	37.0	37.0	37.0
7	36.53402510271931	37.0	37.0	37.0	37.0	37.0
8	36.5354820145234	37.0	37.0	37.0	37.0	37.0
9	36.574322837825086	37.0	37.0	37.0	37.0	37.0
10-11	36.49979265671911	37.0	37.0	37.0	37.0	37.0
12-13	36.49961420553474	37.0	37.0	37.0	37.0	37.0
14-15	38.60896522340975	40.0	37.0	40.0	37.0	40.0
16-17	38.552022358849214	40.0	37.0	40.0	37.0	40.0
18-19	38.567646184924655	40.0	37.0	40.0	37.0	40.0
20-21	38.55027631453076	40.0	37.0	40.0	37.0	40.0
22-23	38.55993169435658	40.0	37.0	40.0	37.0	40.0
24-25	38.54710842917747	40.0	37.0	40.0	37.0	40.0
26-27	38.451641790224194	40.0	37.0	40.0	37.0	40.0
28-29	38.39213002628354	40.0	37.0	40.0	37.0	40.0
30-31	38.34077180145398	40.0	37.0	40.0	37.0	40.0
32-33	38.278260180754685	40.0	37.0	40.0	37.0	40.0
34-35	38.24365293094465	40.0	37.0	40.0	37.0	40.0
36-37	38.19246268713841	40.0	37.0	40.0	37.0	40.0
38-39	38.1445083915358	40.0	37.0	40.0	37.0	40.0
40-41	38.11181409175829	40.0	37.0	40.0	37.0	40.0
42-43	38.0579519461719	40.0	37.0	40.0	33.0	40.0
44-45	37.992613095721815	40.0	37.0	40.0	33.0	40.0
46-47	37.95568408487405	40.0	37.0	40.0	33.0	40.0
48-49	37.93434494269752	40.0	37.0	40.0	33.0	40.0
50-51	37.85998359035571	40.0	37.0	40.0	33.0	40.0
52-53	37.755265760516785	40.0	37.0	40.0	33.0	40.0
54-55	37.56197275692341	40.0	37.0	40.0	33.0	40.0
56-57	37.486851658315345	40.0	37.0	40.0	33.0	40.0
58-59	37.37206397936461	40.0	37.0	40.0	33.0	40.0
60-61	37.23824243670508	37.0	37.0	40.0	33.0	40.0
62-63	37.076673619540216	37.0	37.0	40.0	33.0	40.0
64-65	36.892745351758904	37.0	37.0	40.0	33.0	40.0
66-67	36.691507515926645	37.0	37.0	40.0	33.0	40.0
68-69	36.4906064256091	37.0	37.0	40.0	33.0	40.0
70-71	36.224586800682125	37.0	37.0	40.0	33.0	40.0
72-73	36.003501025976696	37.0	37.0	37.0	33.0	40.0
74-75	35.808999452076854	37.0	37.0	37.0	33.0	40.0
76-77	34.17643098938423	35.0	33.0	37.0	30.0	37.0
78-79	35.26046837883456	37.0	33.0	37.0	33.0	37.0
80-81	35.304596728663384	37.0	33.0	37.0	33.0	37.0
82-83	35.180049806579916	37.0	33.0	37.0	33.0	37.0
84-85	35.01386806995119	37.0	33.0	37.0	33.0	37.0
86-87	34.93115503403966	37.0	33.0	37.0	33.0	37.0
88-89	34.83001464929204	37.0	33.0	37.0	33.0	37.0
90-91	34.71878898292378	37.0	33.0	37.0	33.0	37.0
92-93	34.68875781870032	37.0	33.0	37.0	33.0	37.0
94-95	34.71643821896255	37.0	33.0	37.0	33.0	37.0
96-97	34.70651988263351	37.0	33.0	37.0	33.0	37.0
98-99	34.69723539244575	37.0	33.0	37.0	33.0	37.0
100	34.70938199999609	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	5.0
20	55.0
21	312.0
22	1155.0
23	3295.0
24	7540.0
25	14982.0
26	26453.0
27	41245.0
28	61323.0
29	85661.0
30	116077.0
31	154370.0
32	204357.0
33	274309.0
34	377570.0
35	553355.0
36	977238.0
37	2441647.0
38	3125739.0
39	65052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32012853173647	20.56229997497581	14.509763243041137	23.607808250246578
2	14.43389365247086	21.2218367989198	41.89464171749155	22.449627831117784
3	17.633110225399378	26.714734207363204	30.784194792507275	24.86796077473014
4	12.07837318157071	18.519177495342394	39.505997893748585	29.89645142933831
5	12.993637292253812	38.17719587259113	35.91700173880021	12.91216509635485
6	29.27888298465869	38.530704653777384	19.16429434759819	13.02611801396574
7	26.22234958967948	32.91450928268485	23.48117530470027	17.381965822935403
8	24.384390297647197	36.7721257491873	21.331814300591496	17.511669652574007
9	25.678932035591778	16.735544721507733	21.131316043787056	36.45420719911343
10-11	23.907353180089043	26.85935863013641	29.33970102101649	19.89358716875806
12-13	25.00010841864318	24.401959833998752	29.189645070180852	21.40828667717722
14-15	22.04090832794928	25.87814756086011	26.78357093493095	25.297373176259658
16-17	21.546787907983653	29.01597504379233	27.449050575234025	21.988186472989995
18-19	21.554312746102937	28.08677474537741	29.23190390711396	21.12700860140569
20-21	22.377351889115403	27.23599899080738	29.102747471231122	21.283901648846097
22-23	22.09237575526618	27.25507883557356	28.96270672582883	21.689838683331427
24-25	21.913029246591936	27.42530022150354	29.01338804750787	21.648282484396653
26-27	21.79189219156107	27.606991163741583	28.946569604891703	21.654547039805642
28-29	21.64926684978559	27.580401934890137	29.077572155131403	21.692759060192866
30-31	21.98711628212713	27.426818933055376	28.869558225577745	21.716506559239747
32-33	21.885129680176266	27.545750145753118	28.885509640034595	21.683610534036017
34-35	21.940481909492892	27.679686426699384	28.85366824455493	21.526163419252786
36-37	21.907385601749883	27.595266585855384	28.712676512343528	21.784671300051205
38-39	21.784587507187602	27.637153198105498	28.860736388947732	21.71752290575916
40-41	21.97352206488663	27.55764749524825	28.77261003209975	21.69622040776537
42-43	21.970619608965368	27.585247404452783	28.614282464532153	21.829850522049696
44-45	21.9927112414844	27.778195461963996	28.376060033120066	21.85303326343154
46-47	22.10435589936202	27.7036635917021	28.03068223236015	22.16129827657573
48-49	22.058318900446892	27.848963487125673	28.043234432530838	22.0494831798966
50-51	22.06870836765102	27.921120877897724	27.937798727938755	22.072372026512497
52-53	22.14641574304467	27.924708758664362	27.90070671794179	22.028168780349176
54-55	22.17762741907999	27.86860786006486	27.84679998007327	22.10696474078188
56-57	22.22405402201342	27.964459525829355	27.726681375122592	22.084805077034638
58-59	22.05586651116066	28.087391271992168	27.88233310025593	21.974409116591243
60-61	22.225133477854452	28.126028692073895	27.698484968458835	21.95035286161281
62-63	22.105844019668908	28.219450434913252	27.654241617082278	22.02046392833556
64-65	22.11515606503087	28.12306835598043	27.70077559491881	22.06099998406989
66-67	22.195910683806826	28.04563925062238	27.60465854268083	22.15379152288996
68-69	22.15456943303624	27.995009157742757	27.719900616808413	22.130520792412593
70-71	22.15771737605453	27.83658340631712	27.739401476893207	22.266297740735144
72-73	22.193786068026984	27.887347615169656	27.682410946077784	22.236455370725576
74-75	22.119352411645544	28.01790966146876	27.630501030294386	22.232236896591314
76-77	22.17208235871774	27.968895106418106	27.612349894256266	22.246672640607883
78-79	22.17874376652847	27.950175982970254	27.56071498139217	22.310365269109102
80-81	22.1476021213787	28.017925999279047	27.582531167618146	22.251940711724107
82-83	22.18114559291922	27.97682293892207	27.662257797924887	22.17977367023382
84-85	22.30440517853804	27.965223779961505	27.5474213210652	22.18294972043525
86-87	22.151714201828163	28.0164316143651	27.622827514364335	22.2090266694424
88-89	22.20529059921613	27.89223664726964	27.659858013175377	22.242614740338855
90-91	22.23969133612038	27.972109637498754	27.564829394870205	22.22336963151066
92-93	22.234960279254818	28.0043335542415	27.575369677979772	22.185336488523912
94-95	22.104099358318418	27.993167641651535	27.671944189178998	22.230788810851045
96-97	22.180237054396322	28.114225967821895	27.721216819144622	21.984320158637164
98-99	22.33737777475534	28.82264423844599	26.846002258240496	21.993975728558173
100	20.45644015050795	28.566035460808443	27.997638973061527	22.97988541562208
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1819.0
1	1563.5
2	2036.5
3	2960.5
4	3967.0
5	6358.0
6	8366.0
7	9664.0
8	10420.0
9	10250.5
10	9483.5
11	8707.0
12	9719.5
13	13042.5
14	20594.5
15	31299.0
16	40436.0
17	45927.5
18	46991.0
19	44041.5
20	38967.0
21	33639.0
22	30310.0
23	30891.5
24	35150.0
25	43743.0
26	56257.5
27	70500.5
28	87827.0
29	107901.5
30	127800.0
31	148976.5
32	172095.0
33	195465.5
34	219710.0
35	241628.5
36	263320.5
37	282399.5
38	293866.5
39	304773.5
40	316733.0
41	325300.0
42	328944.5
43	333017.5
44	337718.5
45	341776.0
46	342560.5
47	340436.0
48	342389.0
49	339300.5
50	328756.0
51	318388.0
52	304598.0
53	283433.0
54	260800.0
55	238835.0
56	216077.0
57	191915.5
58	167755.0
59	145448.5
60	121155.5
61	97283.5
62	76552.5
63	58491.5
64	42999.0
65	31161.0
66	23032.0
67	17379.5
68	13179.0
69	9519.5
70	6858.0
71	5082.5
72	3679.5
73	2583.0
74	1819.0
75	1249.5
76	826.5
77	518.0
78	312.0
79	212.0
80	148.0
81	99.0
82	72.5
83	48.5
84	34.0
85	28.0
86	20.5
87	13.0
88	8.5
89	5.5
90	3.5
91	3.0
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0548838484975816E-4
7	0.0
8	0.0
9	0.0
10-11	0.005192372720938096
12-13	2.344186329994626E-5
14-15	0.00441879123203987
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	103.0
20-21	426.0
22-23	924.0
24-25	2219.0
26-27	2661.0
28-29	4203.0
30-31	8575.0
32-33	11017.0
34-35	15410.0
36-37	20712.0
38-39	26176.0
40-41	29794.0
42-43	38527.0
44-45	47160.0
46-47	46049.0
48-49	39578.0
50-51	34596.0
52-53	34547.0
54-55	39153.0
56-57	43218.0
58-59	43447.0
60-61	44989.0
62-63	45684.0
64-65	48328.0
66-67	51692.0
68-69	49329.0
70-71	50125.0
72-73	49082.0
74-75	51069.0
76-77	51442.0
78-79	56339.0
80-81	59337.0
82-83	58453.0
84-85	59862.0
86-87	64066.0
88-89	70715.0
90-91	74428.0
92-93	79730.0
94-95	100317.0
96-97	291442.0
98-99	35478.0
100-101	6651343.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.2127508829094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.48944536805382	71.9274797692772
2	8.537914768748015	14.038509198851873
3	2.20370526973069	5.435180170791716
4	0.8432172483852087	2.77292838326662
5	0.3664471174415538	1.5063312788991354
6	0.19480120378412444	0.9609085703037054
7	0.10445235418676621	0.6011120761723021
8	0.07097535809614566	0.46680635471869686
9	0.0441267282924479	0.32650017483463645
>10	0.14202021001226925	1.7210741035877706
>50	0.0024075063821144345	0.1300022924599626
>100	4.1325566769193246E-4	0.06622614874770759
>500	4.907440175696101E-5	0.024348398372160778
>1k	2.4536817449150688E-5	0.02259307971655224
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.172093164997313E-5	0.0
2	0.0	1.172093164997313E-5	0.0	1.172093164997313E-5	0.0
3	0.0	1.172093164997313E-5	0.0	2.344186329994626E-5	0.0
4	0.0	4.688372659989252E-5	0.0	2.344186329994626E-5	0.0
5	0.0	4.688372659989252E-5	0.0	2.344186329994626E-5	0.0
6	8.20465215498119E-5	4.688372659989252E-5	0.0	2.344186329994626E-5	0.0
7	8.20465215498119E-5	5.860465824986565E-5	0.0	2.344186329994626E-5	0.0
8	8.20465215498119E-5	5.860465824986565E-5	0.0	2.344186329994626E-5	0.0
9	8.20465215498119E-5	1.7581397474959693E-4	0.0	2.344186329994626E-5	0.0
10-11	8.20465215498119E-5	1.7581397474959693E-4	0.0	2.344186329994626E-5	0.0
12-13	8.20465215498119E-5	1.7581397474959693E-4	0.0	2.344186329994626E-5	0.0
14-15	8.20465215498119E-5	2.4613956464943575E-4	0.0	2.9302329124932825E-5	0.0
16-17	2.344186329994626E-4	2.69581427949382E-4	0.0	3.516279494991939E-5	0.0
18-19	2.344186329994626E-4	3.0474422289930136E-4	0.0	3.516279494991939E-5	0.0
20-21	2.4613956464943575E-4	3.457674836742073E-4	0.0	3.516279494991939E-5	0.0
22-23	2.4613956464943575E-4	4.5711633434895204E-4	0.0	3.516279494991939E-5	0.0
24-25	2.4613956464943575E-4	5.567442533737237E-4	0.0	4.688372659989252E-5	0.0
26-27	2.4613956464943575E-4	5.977675141486296E-4	0.0	5.860465824986565E-5	0.0
28-29	2.4613956464943575E-4	5.977675141486296E-4	0.0	1.1134885067474474E-4	0.0
30-31	2.4613956464943575E-4	6.915349673484146E-4	0.0	2.813023595993551E-4	0.0
32-33	2.4613956464943575E-4	6.973954331734012E-4	0.0	6.563721723984953E-4	0.0
34-35	2.4613956464943575E-4	8.028838180231593E-4	0.0	0.0012189768915972054	0.0
36-37	2.4613956464943575E-4	9.02511737047931E-4	0.0	0.0021214886286451363	1.172093164997313E-5
38-39	2.4613956464943575E-4	9.259536003478773E-4	0.0	0.0037506981279914016	1.172093164997313E-5
40-41	2.4613956464943575E-4	0.0010548838484975816	0.0	0.0054092099564626	1.172093164997313E-5
42-43	2.520000304744223E-4	0.0010724652459725413	0.0	0.0071849311014335285	1.172093164997313E-5
44-45	2.8130235959935516E-4	0.0012013954941222458	0.0	0.009189210413578935	1.172093164997313E-5
46-47	2.9302329124932826E-4	0.0013127443447969905	0.0	0.011386885097948895	1.172093164997313E-5
48-49	2.9302329124932826E-4	0.0013244652764469636	0.0	0.014217490091417406	1.172093164997313E-5
50-51	3.2232562037426106E-4	0.0015588839094464262	0.0	0.016590978750536965	1.172093164997313E-5
52-53	3.281860861992476E-4	0.0015706048410963993	0.0	0.019128560452756148	1.172093164997313E-5
54-55	3.281860861992476E-4	0.0015764653069213859	0.0	0.021824374732249966	1.172093164997313E-5
56-57	3.281860861992476E-4	0.0015940467043963457	0.0	0.025047630935992576	1.172093164997313E-5
58-59	3.281860861992476E-4	0.0016057676360463188	0.0	0.02838809645623492	1.172093164997313E-5
60-61	3.5162794949919387E-4	0.0016233490335212785	0.0	0.031412096821927984	1.172093164997313E-5
62-63	3.9851167609908643E-4	0.001711256020896077	0.0	0.03457674836742074	1.172093164997313E-5
64-65	3.9851167609908643E-4	0.002021860709620365	0.0	0.03779414410533836	1.172093164997313E-5
66-67	4.1023260774905953E-4	0.0020511630387452977	0.0	0.04141005151935507	1.172093164997313E-5
68-69	4.1023260774905953E-4	0.00209804676534519	0.0	0.045178331044821426	1.172093164997313E-5
70-71	4.1609307357404614E-4	0.0021214886286451363	0.0	0.04889386637786291	1.172093164997313E-5
72-73	4.6297680017393864E-4	0.0021449304919450825	0.0	0.051771355097931314	1.172093164997313E-5
74-75	4.688372659989252E-4	0.002232837479319881	0.0	0.0542210298127757	1.172093164997313E-5
76-77	4.688372659989252E-4	0.0023793491249445454	0.0	0.05644800682627059	1.172093164997313E-5
78-79	4.688372659989252E-4	0.0024027909882444916	0.0	0.0578838209533923	1.172093164997313E-5
80-81	4.688372659989252E-4	0.0024145119198944647	0.0	0.058458146604240986	1.172093164997313E-5
82-83	4.688372659989252E-4	0.0024320933173694243	0.0	0.058932844336064896	1.172093164997313E-5
84-85	4.688372659989252E-4	0.002449674714844384	0.0	0.05909693737916452	1.172093164997313E-5
86-87	4.8055819764889835E-4	0.0024848375097943037	0.0	0.05913210017411444	1.172093164997313E-5
88	5.040000609488446E-4	0.0025903258946440616	0.0	0.05913210017411444	1.172093164997313E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10640	0.0	49.792637	1
GTATCAA	19420	0.0	37.624805	1
ATCAACG	23220	0.0	30.858994	3
TCAACGC	23305	0.0	30.689793	4
CAACGCA	23780	0.0	29.987278	5
AACGCAG	24585	0.0	29.20295	6
TATCAAC	24975	0.0	28.920332	2
ACGCAGA	27915	0.0	25.65604	7
CGCAGAG	28410	0.0	25.240847	8
GTACATG	25860	0.0	22.31375	1
TACATGG	25505	0.0	22.053097	2
CTTATAC	5330	0.0	21.867655	1
GCAGAGT	33625	0.0	21.104658	9
ACATGGG	26040	0.0	20.92183	3
GAGTACT	19390	0.0	20.004965	12-13
AGAGTAC	30650	0.0	19.25582	10-11
CATGGGG	19025	0.0	18.727375	4
TATACAC	8190	0.0	18.651669	3
CAGAGTA	32945	0.0	18.631542	10-11
GTACTTT	21900	0.0	17.732372	14-15
>>END_MODULE