FastQCFastQC Report
Fri 27 May 2016
SRR2138669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8735298
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC94450.047.1597251
GGTATCA109800.046.6893651
TATACAC129700.037.218433
GTATCAA195150.036.8261951
TTATACA129900.035.157832
TCAACGC232300.030.1715414
ATCAACG234750.029.985813
CAACGCA238600.029.3587275
TATCAAC244950.028.896522
AACGCAG247450.028.3788326
TACACAT186450.025.6129765
ACGCAGA280700.025.0016567
CGCAGAG283350.024.7372158
ACACATC184100.024.5263046
ACATCTC187550.024.0056218
CACATCT202850.022.1522277
ATACACA249500.020.894854
GTACATG248250.020.8427011
GCAGAGT335250.020.8315739
TACATGG246450.020.2557962
Produced by FastQC (version 0.11.5)