FastQCFastQC Report
Fri 27 May 2016
SRR2138668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7233167
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92600.046.679681
CTTATAC65800.042.077711
GTATCAA163950.037.5071
TATACAC93250.033.656433
ATCAACG191050.031.3112033
TCAACGC191350.031.2855474
CAACGCA194650.030.7145925
AACGCAG200100.029.9440446
TTATACA96900.029.8902442
TATCAAC201500.029.8839782
ACGCAGA227250.026.3084537
CGCAGAG231650.025.7906328
TACACAT133850.022.9581265
ACACATC130450.021.936576
GTACATG219650.021.88361
ACATCTC132350.021.5891328
GCAGAGT277600.021.4603989
TACATGG219000.021.2651732
ACATGGG218950.020.526183
GAGTACT148200.020.11073112-13
Produced by FastQC (version 0.11.5)