##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138668_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7233167
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62739474976867	33.0	33.0	33.0	33.0	33.0
2	32.641012159680535	33.0	33.0	33.0	33.0	33.0
3	32.641901949726865	33.0	33.0	33.0	33.0	33.0
4	36.550633491525915	37.0	37.0	37.0	37.0	37.0
5	36.57121133799344	37.0	37.0	37.0	37.0	37.0
6	36.552998845457324	37.0	37.0	37.0	37.0	37.0
7	36.51746558595979	37.0	37.0	37.0	37.0	37.0
8	36.51642454819583	37.0	37.0	37.0	37.0	37.0
9	36.55698022733334	37.0	37.0	37.0	37.0	37.0
10-11	36.480339801362256	37.0	37.0	37.0	37.0	37.0
12-13	36.479417038207465	37.0	37.0	37.0	37.0	37.0
14-15	38.57028069447311	40.0	37.0	40.0	37.0	40.0
16-17	38.50643556273483	40.0	37.0	40.0	37.0	40.0
18-19	38.52128230967155	40.0	37.0	40.0	37.0	40.0
20-21	38.50185973229647	40.0	37.0	40.0	37.0	40.0
22-23	38.508169560116244	40.0	37.0	40.0	37.0	40.0
24-25	38.49802044185628	40.0	37.0	40.0	37.0	40.0
26-27	38.389701409584205	40.0	37.0	40.0	37.0	40.0
28-29	38.32174499341167	40.0	37.0	40.0	37.0	40.0
30-31	38.275736320813806	40.0	37.0	40.0	37.0	40.0
32-33	38.21266427118698	40.0	37.0	40.0	37.0	40.0
34-35	38.17925891694372	40.0	37.0	40.0	37.0	40.0
36-37	38.12370876890033	40.0	37.0	40.0	37.0	40.0
38-39	38.06080233089355	40.0	37.0	40.0	33.0	40.0
40-41	38.04286649981745	40.0	37.0	40.0	33.0	40.0
42-43	38.00160237752358	40.0	37.0	40.0	33.0	40.0
44-45	37.93923289659318	40.0	37.0	40.0	33.0	40.0
46-47	37.90554084691449	40.0	37.0	40.0	33.0	40.0
48-49	37.88286500309327	40.0	37.0	40.0	33.0	40.0
50-51	37.78719829821167	40.0	37.0	40.0	33.0	40.0
52-53	37.685331974642395	40.0	37.0	40.0	33.0	40.0
54-55	37.49884877771339	40.0	37.0	40.0	33.0	40.0
56-57	37.419089092410516	40.0	37.0	40.0	33.0	40.0
58-59	37.30332230281505	40.0	37.0	40.0	33.0	40.0
60-61	37.16319019851998	37.0	37.0	40.0	33.0	40.0
62-63	37.00937578398965	37.0	37.0	40.0	33.0	40.0
64-65	36.83101814207512	37.0	37.0	40.0	33.0	40.0
66-67	36.631502098371676	37.0	37.0	40.0	33.0	40.0
68-69	36.43128282314194	37.0	37.0	40.0	33.0	40.0
70-71	36.163756614554316	37.0	37.0	38.5	33.0	40.0
72-73	35.939277443009416	37.0	37.0	37.0	33.0	40.0
74-75	35.743546858218245	37.0	35.0	37.0	33.0	40.0
76-77	34.123527923032725	35.0	33.0	37.0	30.0	37.0
78-79	35.201968277342985	37.0	33.0	37.0	33.0	37.0
80-81	35.250341882106994	37.0	33.0	37.0	33.0	37.0
82-83	35.120293655972674	37.0	33.0	37.0	33.0	37.0
84-85	34.95515576207632	37.0	33.0	37.0	33.0	37.0
86-87	34.87344110955512	37.0	33.0	37.0	33.0	37.0
88-89	34.77153730502859	37.0	33.0	37.0	33.0	37.0
90-91	34.65487878529733	37.0	33.0	37.0	33.0	37.0
92-93	34.62794391132414	37.0	33.0	37.0	33.0	37.0
94-95	34.654289260676705	37.0	33.0	37.0	33.0	37.0
96-97	34.64855028788626	37.0	33.0	37.0	33.0	37.0
98-99	34.64002133370473	37.0	33.0	37.0	33.0	37.0
100	34.65172467445381	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	7.0
20	49.0
21	295.0
22	1094.0
23	3132.0
24	7223.0
25	14163.0
26	24330.0
27	38063.0
28	55543.0
29	76625.0
30	102709.0
31	135395.0
32	178395.0
33	239773.0
34	330071.0
35	481264.0
36	846011.0
37	2078458.0
38	2571819.0
39	48747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.74865131691277	20.639520696812337	14.615105665333042	23.99672232094185
2	14.137790541819372	21.193413618128822	42.0628197855794	22.605976054472404
3	17.426612713352256	26.939624095503394	31.091498371321997	24.542264819822353
4	12.023737320042521	18.802165082044976	39.17584095597405	29.998256641938447
5	12.984174152207464	38.16509421115259	35.89426319066047	12.956468445979471
6	29.059695770547833	38.40580581734758	19.268621082434382	13.265877329670204
7	25.900079453439968	33.00613686923031	23.36011321181994	17.733670465509782
8	24.34596076656325	36.577172903653405	21.53225551131337	17.544610818469973
9	25.495802875835714	16.759906137933772	20.960044196408017	36.7842467898225
10-11	23.854042975000024	26.740675948336097	29.24818118162343	20.157099895040453
12-13	24.974855412573774	24.269064989098137	28.9720533204888	21.784026277839292
14-15	22.135132823986744	25.790857463536387	26.43499395087269	25.639015761604174
16-17	21.735790145589064	28.97893135883632	27.06251632237995	22.222762173194674
18-19	21.763177872154756	27.92822286558571	28.680376106344564	21.62822315591497
20-21	22.638546208061328	27.10042109763	28.624947151957098	21.636085542351573
22-23	22.147450058517197	27.437288363847607	28.374939223900704	22.040322353734492
24-25	22.112539619322614	27.591532389660532	28.577970550328295	21.717957440688558
26-27	22.10809517288505	27.760883728457664	28.452251026023013	21.67877007263427
28-29	21.60834027916104	27.456382427506764	28.785823191456256	22.149454101875936
30-31	22.23881485707402	27.257034137871113	28.805115661104068	21.699035343950804
32-33	21.918799389990294	27.67434493276029	28.57369333148482	21.833162345764592
34-35	21.873458665576532	27.74181870796739	28.873803868943803	21.510918757512272
36-37	22.250978527334368	27.4860780215291	28.350931203984373	21.912012247152163
38-39	21.978031795380335	27.44031526249726	28.60015232122357	21.98150062089883
40-41	22.103017459000178	27.269111068648606	28.661489727840877	21.966381744510336
42-43	22.146578113267417	27.269855998399912	28.583620321709695	21.999945566622973
44-45	22.049152332478155	27.578733189289323	28.269525248509847	22.10258922972268
46-47	22.369421384364145	27.334927779603813	27.795664877909253	22.499985958122785
48-49	22.21414614899633	27.419324282173758	28.038298459781547	22.328231109048364
50-51	22.121637997947587	27.70542147571893	27.926051401097013	22.24688912523647
52-53	22.16539599059223	27.81216418289047	28.004340603586954	22.01809922293035
54-55	22.23260176079553	27.74879992074774	27.753405080513787	22.26519323794295
56-57	22.42796482624059	27.93938222574257	27.454508057563327	22.178144890453517
58-59	22.073025463962022	28.162954664413814	27.875010789814414	21.889009081809753
60-61	22.403891826302484	28.319805914516394	27.368768704765888	21.90753355441523
62-63	22.19105152297407	28.425639824253953	27.342850006873228	22.040458645898756
64-65	22.17954498682108	28.20057515643403	27.49002206186322	22.129857794881673
66-67	22.329368911699653	28.02331699220593	27.413175025466032	22.234139070628384
68-69	22.298923808188345	27.89185416222682	27.522684770912363	22.286537258672467
70-71	22.304813935208536	27.698928714388384	27.59744091550261	22.398816434900475
72-73	22.333265275515597	27.751745405866334	27.53954975288237	22.375439565735693
74-75	22.235074909449988	27.904124391022417	27.470566890235283	22.39023380929231
76-77	22.339749000652766	27.826286258107874	27.44276720808898	22.391197533150375
78-79	22.341422882764242	27.817969460383157	27.415357601981626	22.425250054870975
80-81	22.301724690342056	27.885558429145078	27.437055920725545	22.375660959787318
82-83	22.35978486740088	27.81224349218643	27.551214942591123	22.27675669782157
84-85	22.454725758742335	27.7806895732773	27.440521620201615	22.32406304777875
86-87	22.26131495312271	27.82384284571725	27.549488928277157	22.36535327288289
88-89	22.36688576739427	27.743577166330653	27.522732536559598	22.366804529715484
90-91	22.39257702618681	27.83361132416047	27.481206424190045	22.292605225462676
92-93	22.379718434195542	27.82117547171934	27.52288698257783	22.276219111507285
94-95	22.2293474873367	27.86225828715426	27.57042058068598	22.337973644823062
96-97	22.305585145952243	28.00330630729608	27.626469525375136	22.064639021376546
98-99	22.53418238306245	28.671932526017674	26.70816597121161	22.085719119708262
100	20.58136678895434	28.50989318051568	27.831510241578115	23.07722978895186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1132.0
1	1031.0
2	1476.5
3	2127.5
4	2928.5
5	4864.0
6	6401.0
7	7282.5
8	7799.5
9	7566.5
10	6916.5
11	6257.0
12	6786.5
13	9225.0
14	14936.5
15	22932.0
16	29813.0
17	34177.5
18	35518.0
19	33688.0
20	29751.0
21	25691.5
22	23065.5
23	23380.0
24	26832.5
25	32990.5
26	42111.0
27	53632.0
28	66938.5
29	82348.0
30	99047.5
31	116523.5
32	136660.0
33	157659.0
34	178331.5
35	197908.0
36	218541.5
37	238300.5
38	250055.0
39	259644.5
40	270575.5
41	278396.0
42	283764.0
43	290234.0
44	295954.5
45	301078.5
46	310072.0
47	318841.5
48	316665.5
49	309854.5
50	303721.5
51	289982.0
52	268987.0
53	246018.5
54	224731.0
55	204180.5
56	184199.0
57	162296.5
58	140841.5
59	121152.0
60	100291.0
61	80550.5
62	63393.5
63	48478.0
64	35610.5
65	26287.0
66	19688.5
67	14872.5
68	11066.5
69	7831.0
70	5671.0
71	4190.0
72	2937.0
73	1926.5
74	1242.0
75	817.0
76	524.5
77	331.0
78	213.5
79	137.0
80	93.0
81	61.0
82	46.5
83	38.0
84	30.0
85	23.0
86	16.0
87	9.0
88	6.0
89	6.0
90	5.0
91	3.5
92	3.0
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.1060162166862731E-4
7	0.0
8	0.0
9	0.0
10-11	0.005260489630614086
12-13	0.0
14-15	0.004223599427470705
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	101.0
20-21	400.0
22-23	828.0
24-25	1916.0
26-27	2372.0
28-29	3879.0
30-31	8255.0
32-33	10632.0
34-35	14452.0
36-37	20403.0
38-39	25052.0
40-41	27431.0
42-43	33799.0
44-45	40994.0
46-47	39573.0
48-49	35463.0
50-51	31333.0
52-53	32429.0
54-55	41358.0
56-57	45605.0
58-59	44267.0
60-61	47110.0
62-63	47747.0
64-65	46201.0
66-67	50075.0
68-69	38907.0
70-71	38767.0
72-73	37450.0
74-75	38908.0
76-77	38455.0
78-79	42157.0
80-81	44100.0
82-83	43409.0
84-85	44216.0
86-87	47402.0
88-89	52689.0
90-91	55941.0
92-93	60319.0
94-95	78452.0
96-97	245239.0
98-99	32103.0
100-101	5642978.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.60468271557033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.14227888119174	73.69107259689352
2	8.241162336541773	13.779995247081667
3	2.102991113876368	5.274597144878927
4	0.746788736225099	2.497401413906446
5	0.3296038770608437	1.3778213781746844
6	0.1554881019220774	0.7799720056344925
7	0.09400787054769424	0.550164873293447
8	0.05464620345328276	0.3654942801057767
9	0.035141873980415086	0.2644222701746844
>10	0.09515767141080465	1.19433572576358
>50	0.002183556866160302	0.11599998046189851
>100	5.231055837520535E-4	0.09458584151684632
>500	2.6671339947141335E-5	0.01413724211399836
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3825202708578414E-5	0.0	0.0	0.0
3	0.0	1.3825202708578414E-5	0.0	0.0	0.0
4	0.0	2.7650405417156827E-5	0.0	1.3825202708578414E-5	1.3825202708578414E-5
5	0.0	2.7650405417156827E-5	0.0	1.3825202708578414E-5	1.3825202708578414E-5
6	0.0	2.7650405417156827E-5	0.0	1.3825202708578414E-5	1.3825202708578414E-5
7	0.0	2.7650405417156827E-5	0.0	1.3825202708578414E-5	1.3825202708578414E-5
8	0.0	4.147560812573524E-5	0.0	1.3825202708578414E-5	1.3825202708578414E-5
9	0.0	8.295121625147048E-5	0.0	1.3825202708578414E-5	1.3825202708578414E-5
10-11	0.0	8.295121625147048E-5	0.0	1.3825202708578414E-5	1.3825202708578414E-5
12-13	1.3825202708578414E-5	8.986381760575968E-5	0.0	1.3825202708578414E-5	1.3825202708578414E-5
14-15	1.3825202708578414E-5	1.5898983114865175E-4	0.0	1.3825202708578414E-5	1.3825202708578414E-5
16-17	1.3825202708578414E-5	1.797276352115194E-4	0.0	1.3825202708578414E-5	1.3825202708578414E-5
18-19	1.3825202708578414E-5	1.935528379200978E-4	0.0	1.3825202708578414E-5	1.3825202708578414E-5
20-21	1.3825202708578414E-5	2.4885364875441146E-4	0.0	2.073780406286762E-5	1.3825202708578414E-5
22-23	2.7650405417156827E-5	2.765040541715683E-4	0.0	2.7650405417156827E-5	1.3825202708578414E-5
24-25	2.7650405417156827E-5	3.041544595887251E-4	0.0	5.5300810834313655E-5	1.3825202708578414E-5
26-27	2.7650405417156827E-5	3.179796622973035E-4	0.0	6.221341218860287E-5	1.3825202708578414E-5
28-29	2.7650405417156827E-5	3.248922636515927E-4	0.0	1.3133942573149494E-4	1.3825202708578414E-5
30-31	2.7650405417156827E-5	3.456300677144603E-4	0.0	3.318048650058819E-4	1.3825202708578414E-5
32-33	4.147560812573524E-5	3.456300677144603E-4	0.0	7.603861489718128E-4	2.7650405417156827E-5
34-35	1.5207722979436255E-4	4.078434799030632E-4	0.0	0.0015000344938807578	2.7650405417156827E-5
36-37	1.5207722979436255E-4	4.907946961545337E-4	0.0	0.002737390136298526	2.7650405417156827E-5
38-39	1.797276352115194E-4	5.253577029259797E-4	0.0	0.004714394123625239	2.7650405417156827E-5
40-41	2.2120324333725462E-4	5.737459124060041E-4	0.0	0.0068227375366834465	2.7650405417156827E-5
42-43	2.2120324333725462E-4	6.221341218860286E-4	0.0	0.008675314699632954	2.7650405417156827E-5
44-45	2.2120324333725462E-4	7.050853381374992E-4	0.0	0.010756007707274005	2.7650405417156827E-5
46-47	2.3502844604583303E-4	7.603861489718128E-4	0.0	0.013203068586692386	2.7650405417156827E-5
48-49	2.4885364875441146E-4	7.880365543889696E-4	0.0	0.015643216864756476	4.147560812573524E-5
50-51	2.765040541715683E-4	9.33201182829043E-4	0.0	0.018311480987512112	4.147560812573524E-5
52-53	2.765040541715683E-4	9.677641896004889E-4	0.0	0.020938269502142007	4.8388209480024444E-5
54-55	2.834166555258575E-4	9.885019936633566E-4	0.0	0.023848474672297764	6.221341218860287E-5
56-57	2.903292568801467E-4	0.0010092397977262242	0.0	0.026876194065476436	6.912601354289207E-5
58-59	2.903292568801467E-4	0.0010299776017890917	0.0	0.02990391345865511	7.603861489718127E-5
60-61	3.041544595887251E-4	0.001071453209914827	0.0	0.033152836095171036	8.295121625147048E-5
62-63	3.041544595887251E-4	0.0011267540207491407	0.0	0.03580727501521809	9.67764189600489E-5
64-65	3.041544595887251E-4	0.0013203068586692384	0.0	0.038841907009751056	9.67764189600489E-5
66-67	3.041544595887251E-4	0.0013410446627321062	0.0	0.04202861623407838	9.67764189600489E-5
68-69	3.041544595887251E-4	0.0013548698654406847	0.0	0.045471091708514405	9.67764189600489E-5
70-71	3.041544595887251E-4	0.001368695068149263	0.0	0.04843659768950448	1.1060162166862731E-4
72-73	3.041544595887251E-4	0.001375607669503552	0.0	0.05094587198111146	1.1060162166862731E-4
74-75	3.041544595887251E-4	0.0014101706762749983	0.0	0.05306804059687824	1.1060162166862731E-4
76-77	3.041544595887251E-4	0.0014516462844007334	0.0	0.05511417059774784	1.2442682437720573E-4
78-79	3.041544595887251E-4	0.0014792966898178903	0.0	0.05662803029433718	1.2442682437720573E-4
80-81	3.041544595887251E-4	0.0014792966898178903	0.0	0.057132650193200296	1.3133942573149494E-4
82-83	3.110670609430143E-4	0.0014792966898178903	0.0	0.05762344488935483	1.3825202708578414E-4
84-85	3.179796622973035E-4	0.0014792966898178903	0.0	0.0577686095177949	1.3825202708578414E-4
86-87	3.179796622973035E-4	0.001506947095235047	0.0	0.057851560734046376	1.3825202708578414E-4
88	3.179796622973035E-4	0.0015622479060693607	0.0	0.05787229853810924	1.3825202708578414E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9260	0.0	46.67968	1
CTTATAC	6580	0.0	42.07771	1
GTATCAA	16395	0.0	37.507	1
TATACAC	9325	0.0	33.65643	3
ATCAACG	19105	0.0	31.311203	3
TCAACGC	19135	0.0	31.285547	4
CAACGCA	19465	0.0	30.714592	5
AACGCAG	20010	0.0	29.944044	6
TTATACA	9690	0.0	29.890244	2
TATCAAC	20150	0.0	29.883978	2
ACGCAGA	22725	0.0	26.308453	7
CGCAGAG	23165	0.0	25.790632	8
TACACAT	13385	0.0	22.958126	5
ACACATC	13045	0.0	21.93657	6
GTACATG	21965	0.0	21.8836	1
ACATCTC	13235	0.0	21.589132	8
GCAGAGT	27760	0.0	21.460398	9
TACATGG	21900	0.0	21.265173	2
ACATGGG	21895	0.0	20.52618	3
GAGTACT	14820	0.0	20.110731	12-13
>>END_MODULE