FastQCFastQC Report
Fri 27 May 2016
SRR2138667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5660356
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC108650.054.1045841
GGTATCA73550.048.814231
TATACAC140150.043.537663
TTATACA136050.043.486872
GTATCAA129850.035.730161
TACACAT195750.030.9503925
TCAACGC155050.029.3250834
ATCAACG155500.029.1318233
CAACGCA160000.028.3072385
TATCAAC161950.028.2057672
ATACACA222350.028.1625024
ACACATC211050.027.9478156
AACGCAG165650.027.4940936
ACATCTC224500.026.2546678
CACATCT231300.025.2460127
ACGCAGA187900.024.1038747
CGCAGAG191200.023.709898
CATCTCC260350.022.5261429
GTGGTAT39900.021.9674681
TGGTATC38150.020.6555422
Produced by FastQC (version 0.11.5)