##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138667_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5660356
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82593956987864	33.0	33.0	33.0	33.0	33.0
2	32.83137474038735	33.0	33.0	33.0	33.0	33.0
3	32.83070640786551	33.0	33.0	33.0	33.0	33.0
4	36.78102119371997	37.0	37.0	37.0	37.0	37.0
5	36.7903213154791	37.0	37.0	37.0	37.0	37.0
6	36.796835746726885	37.0	37.0	37.0	37.0	37.0
7	36.78281719383021	37.0	37.0	37.0	37.0	37.0
8	36.77948418792034	37.0	37.0	37.0	37.0	37.0
9	36.779044286260444	37.0	37.0	37.0	37.0	37.0
10-11	36.75731764927859	37.0	37.0	37.0	37.0	37.0
12-13	36.74167322691365	37.0	37.0	37.0	37.0	37.0
14-15	39.0775411475886	40.0	40.0	40.0	37.0	40.0
16-17	39.02958789164498	40.0	40.0	40.0	37.0	40.0
18-19	39.05528044525821	40.0	40.0	40.0	37.0	40.0
20-21	39.08038941260423	40.0	40.0	40.0	37.0	40.0
22-23	39.09811611601354	40.0	40.0	40.0	37.0	40.0
24-25	39.0058720226682	40.0	40.0	40.0	37.0	40.0
26-27	38.90434375419228	40.0	40.0	40.0	37.0	40.0
28-29	38.85840097826314	40.0	40.0	40.0	37.0	40.0
30-31	38.81041527995414	40.0	38.5	40.0	37.0	40.0
32-33	38.66168565726086	40.0	37.0	40.0	37.0	40.0
34-35	38.7456026042277	40.0	37.0	40.0	37.0	40.0
36-37	38.40705776288971	40.0	37.0	40.0	37.0	40.0
38-39	38.58500145111802	40.0	37.0	40.0	37.0	40.0
40-41	38.61620880650284	40.0	37.0	40.0	37.0	40.0
42-43	38.59398498046346	40.0	37.0	40.0	37.0	40.0
44-45	38.51550955996515	40.0	37.0	40.0	37.0	40.0
46-47	38.440770819665104	40.0	37.0	40.0	37.0	40.0
48-49	38.408937909356375	40.0	37.0	40.0	37.0	40.0
50-51	38.268837211235116	40.0	37.0	40.0	37.0	40.0
52-53	38.193447342352826	40.0	37.0	40.0	37.0	40.0
54-55	38.12244231990037	40.0	37.0	40.0	37.0	40.0
56-57	37.97753012842291	40.0	37.0	40.0	35.0	40.0
58-59	37.85850041653693	40.0	37.0	40.0	33.0	40.0
60-61	37.69387755386444	40.0	37.0	40.0	33.0	40.0
62-63	37.50406593051307	37.0	37.0	40.0	33.0	40.0
64-65	37.30366855308429	37.0	37.0	40.0	33.0	40.0
66-67	37.09029966377187	37.0	37.0	40.0	33.0	40.0
68-69	36.857236411996205	37.0	37.0	40.0	33.0	40.0
70-71	36.62258961370929	37.0	37.0	40.0	33.0	40.0
72-73	36.381500724328234	37.0	37.0	37.0	33.0	40.0
74-75	36.13619410092718	37.0	37.0	37.0	33.0	40.0
76-77	34.46934581889653	35.0	33.0	37.0	30.0	37.0
78-79	35.75642046701133	37.0	35.0	37.0	33.0	37.0
80-81	35.87382587005651	37.0	37.0	37.0	33.0	37.0
82-83	35.794618630098725	37.0	37.0	37.0	33.0	37.0
84-85	35.68118740436709	37.0	37.0	37.0	33.0	37.0
86-87	35.60243087831512	37.0	37.0	37.0	33.0	37.0
88-89	35.529251989961	37.0	37.0	37.0	33.0	37.0
90-91	35.460754618747785	37.0	33.0	37.0	33.0	37.0
92-93	35.399532035045894	37.0	33.0	37.0	33.0	37.0
94-95	35.35849625292113	37.0	33.0	37.0	33.0	37.0
96-97	35.3191904312296	37.0	33.0	37.0	33.0	37.0
98-99	35.281688499616656	37.0	33.0	37.0	33.0	37.0
100	35.19276445808982	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	19.0
21	106.0
22	458.0
23	1376.0
24	3193.0
25	6281.0
26	11120.0
27	17536.0
28	25334.0
29	35085.0
30	46244.0
31	61008.0
32	81649.0
33	111859.0
34	162239.0
35	253835.0
36	513986.0
37	1640036.0
38	2580284.0
39	108701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.48535109805814	20.24181871246261	14.419658410177735	24.853171779301515
2	14.116832934182938	20.33183071877458	42.22532999691185	23.32600635013063
3	17.510904432316728	26.07175610253355	31.394571289608898	25.022768175540822
4	11.994660102822175	18.4346962557991	37.21256307113001	32.35808057024872
5	13.135574954578445	37.4202958566919	35.510311453016335	13.933817735713323
6	29.789539739196613	37.28398001821794	18.48207073901359	14.444409503571858
7	25.931195847045664	32.9083188407231	21.941499792592552	19.218985519638693
8	24.667194077545656	36.199030591008764	21.229795440428127	17.903979891017453
9	24.65124101734944	16.6351021031186	20.329251375708523	38.38440550382343
10-11	23.859550530037332	26.30263891529084	28.457715380446036	21.38009517422579
12-13	24.996493153434166	23.83832394994237	28.23274719823276	22.9324356983907
14-15	22.40753938444861	25.555318428734868	25.404762527303937	26.632379659512583
16-17	22.274703216546804	29.0775350525656	25.97634318406828	22.671418546819318
18-19	22.538732546150808	27.266014717095533	27.82544066132943	22.369812075424232
20-21	22.653497385182934	26.775131385701993	28.09214198047681	22.479229248638266
22-23	22.61824771103683	27.682924911619107	27.70223628534684	21.996591091997228
24-25	23.377160120012977	27.370800171043147	27.88247155046864	21.36956815847524
26-27	22.903584034353273	27.976768950532005	28.04070907922412	21.0789379358906
28-29	22.598564142874025	27.128617692086053	28.15713965151187	22.115678513528046
30-31	22.263745334077857	27.45768401530244	28.693871463410247	21.584699187209456
32-33	22.692282798242864	27.565474583354455	28.42598283902201	21.31625977938067
34-35	22.93059882487848	27.03182740956793	28.80841403102858	21.229159734525012
36-37	22.10824753279029	27.641677590879667	28.019406443198697	22.23066843313135
38-39	22.17234505900588	26.508323526884407	28.636682482823883	22.682648931285826
40-41	21.962029878955416	26.347518349523774	29.127165146120994	22.56328662539982
42-43	22.3447501278971	26.21000580965579	29.33169656607553	22.113547496371584
44-45	21.836831576388445	26.808341235463345	28.855850190114555	22.49897699803365
46-47	22.883983192427593	26.173938262228575	27.737818834311256	23.204259711032577
48-49	22.382073108308305	26.346049877672794	28.34967211153559	22.922204902483312
50-51	22.09472003307622	27.056426229887837	28.24778585782553	22.601067879210415
52-53	21.966884051512434	27.596178097544417	28.591173631641727	21.845764219301422
54-55	21.92458547696223	27.741910887400493	27.796068739959466	22.537434895677816
56-57	22.59404988140499	28.17684666749376	27.000259335999356	22.228844115101897
58-59	21.713567660663486	28.745041622040574	27.978733722588046	21.5626569947079
60-61	22.526267260545943	29.207964327585394	26.691968501595	21.573799910273667
62-63	21.890161800193123	29.649407805307042	26.672253718580112	21.78817667591972
64-65	21.9921436982564	29.147614997408837	26.87327279382409	21.986968510510675
66-67	22.214134838815287	28.508444108340612	26.999445352750783	22.277975700093318
68-69	22.33773296423638	27.98418240037584	27.22289102088983	22.45519361449795
70-71	22.399202107070273	27.577647598281878	27.391201058979764	22.631949235668085
72-73	22.53363578710633	27.553294817433926	27.29199338286706	22.62107601259268
74-75	22.34805060916739	27.736643886345018	27.297639125383665	22.617666379103923
76-77	22.4899599707974	27.610658511583946	27.23465107216559	22.664730445453067
78-79	22.4706900765331	27.61344869031707	27.216363006759604	22.699498226390226
80-81	22.47479239719911	27.685155758455632	27.220879697089018	22.619172147256243
82-83	22.499805229053436	27.635559177578976	27.339451289607865	22.525184303759723
84-85	22.66102931208628	27.643771665413258	27.179350752191688	22.51584827030877
86-87	22.521662427527854	27.63706120631332	27.314087357660565	22.52718900849826
88-89	22.564823355103833	27.608270223777943	27.25004939108913	22.57685703002909
90-91	22.625025581508307	27.689937093658777	27.215090719570412	22.469946605262507
92-93	22.648795124247	27.5691029257062	27.321678341444983	22.460423608601822
94-95	22.468450606126407	27.667095644189722	27.371618405304858	22.49283534437901
96-97	22.532163921326763	27.794954334844192	27.37831676044073	22.29456498338832
98-99	23.71843889288367	29.720675502129524	27.469124070733685	19.091761534253127
100	22.56961404252911	30.666028962606408	21.423999393430257	25.34035760143423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1362.0
1	1250.5
2	1562.0
3	2157.5
4	2955.0
5	4733.0
6	6262.5
7	7447.5
8	8305.5
9	8297.0
10	7823.0
11	7242.5
12	7729.5
13	9856.5
14	14825.0
15	21585.0
16	26962.5
17	30042.0
18	30603.0
19	29053.5
20	26395.5
21	23767.5
22	22691.5
23	24173.0
24	28188.5
25	34567.5
26	42770.0
27	51852.0
28	61938.0
29	73357.0
30	84637.5
31	96001.0
32	107702.0
33	119306.0
34	130967.0
35	142336.0
36	154639.0
37	166414.0
38	175586.0
39	183141.5
40	190486.0
41	196997.5
42	202875.5
43	209775.5
44	220366.0
45	242667.5
46	269730.0
47	290765.0
48	288528.5
49	275879.5
50	269063.0
51	250095.0
52	226501.5
53	208669.0
54	195890.5
55	182420.0
56	167694.5
57	152428.0
58	136388.0
59	118911.0
60	98633.0
61	80388.5
62	64558.5
63	49830.5
64	37347.5
65	27945.0
66	21011.5
67	15933.0
68	12146.0
69	8623.0
70	5918.0
71	4231.5
72	2857.0
73	1767.0
74	1120.5
75	715.0
76	448.0
77	283.0
78	192.0
79	126.5
80	85.0
81	62.0
82	48.5
83	31.5
84	18.5
85	10.5
86	7.0
87	6.0
88	4.5
89	1.5
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	7.596695331530385E-4
4	0.018921071395509397
5	8.480031997987405E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.007872878895329944
26-27	0.050984595810512325
28-29	0.08441949546416572
30-31	0.07460614155986255
32-33	0.03512892305887183
34-35	0.00449511932405511
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.8664977407698056E-4
56-57	7.343290319688212E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.1902095446603285E-5
72-73	0.013859768535429034
74-75	0.028903006507611434
76-77	0.003981815430745702
78-79	0.0
80-81	0.0
82-83	0.010849946473223777
84-85	0.04964016254467077
86-87	0.040108711271951786
88-89	0.010096202053124923
90-91	1.1647798979489741E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	66.0
20-21	283.0
22-23	664.0
24-25	2183.0
26-27	2753.0
28-29	5249.0
30-31	12119.0
32-33	15340.0
34-35	20575.0
36-37	29869.0
38-39	34503.0
40-41	35564.0
42-43	40901.0
44-45	48498.0
46-47	46997.0
48-49	43379.0
50-51	38995.0
52-53	38238.0
54-55	51612.0
56-57	66500.0
58-59	67319.0
60-61	64784.0
62-63	68893.0
64-65	73363.0
66-67	79921.0
68-69	58789.0
70-71	42529.0
72-73	38436.0
74-75	39187.0
76-77	38668.0
78-79	40922.0
80-81	42169.0
82-83	40497.0
84-85	39630.0
86-87	41756.0
88-89	44735.0
90-91	47167.0
92-93	48732.0
94-95	58570.0
96-97	173382.0
98-99	336477.0
100-101	3640142.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.22933779882791
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.03495194324694	77.63654284815479
2	7.190878900267621	12.401294515232816
3	1.6460169060033023	4.2580484343102105
4	0.5761812198892579	1.9873490017268625
5	0.23554810947814583	1.0155578750033167
6	0.11576243641763367	0.5989270940562884
7	0.06674461590480146	0.4028740821775703
8	0.035195159251017676	0.24278842207516368
9	0.022035089052964228	0.17100640236377765
>10	0.07387545403268521	0.9804311009921283
>50	0.0017622144340147007	0.10156804352253646
>100	9.854298108960672E-4	0.17169598733508443
>500	6.252221069575066E-5	0.03191619304952012
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.3000199987421284E-5	0.0	0.0	0.0
3	0.0	5.3000199987421284E-5	0.0	0.0	0.0
4	0.0	8.833366664570214E-5	0.0	0.0	0.0
5	0.0	8.833366664570214E-5	0.0	0.0	0.0
6	0.0	8.833366664570214E-5	0.0	0.0	0.0
7	0.0	8.833366664570214E-5	0.0	0.0	0.0
8	0.0	1.4133386663312344E-4	0.0	0.0	0.0
9	0.0	2.2966753327882558E-4	0.0	1.766673332914043E-5	0.0
10-11	1.766673332914043E-5	2.2966753327882558E-4	0.0	1.766673332914043E-5	0.0
12-13	1.766673332914043E-5	2.2966753327882558E-4	0.0	2.6500099993710645E-5	0.0
14-15	1.766673332914043E-5	3.180011999245277E-4	0.0	3.533346665828086E-5	0.0
16-17	1.766673332914043E-5	3.4450129991823834E-4	0.0	5.3000199987421284E-5	0.0
18-19	1.766673332914043E-5	3.886681332410894E-4	0.0	6.18335666519915E-5	0.0
20-21	1.766673332914043E-5	4.328349665639405E-4	0.0	8.833366664570214E-5	0.0
22-23	1.766673332914043E-5	5.035018998805022E-4	0.0	8.833366664570214E-5	0.0
24-25	1.766673332914043E-5	5.476687332033534E-4	0.0	1.0600039997484257E-4	0.0
26-27	1.766673332914043E-5	5.653354665324938E-4	0.0	1.6783396662683407E-4	0.0
28-29	1.766673332914043E-5	5.653354665324938E-4	0.0	6.890025998364767E-4	0.0
30-31	1.766673332914043E-5	6.18335666519915E-4	0.0	0.0025793430660545026	0.0
32-33	1.766673332914043E-5	6.360023998490554E-4	0.0	0.006095022998553448	0.0
34-35	1.766673332914043E-5	7.773362664821788E-4	0.0	0.010229038597572309	0.0
36-37	1.766673332914043E-5	8.833366664570214E-4	0.0	0.016253394662809194	0.0
38-39	1.766673332914043E-5	8.833366664570214E-4	0.0	0.030448614892773528	0.0
40-41	1.766673332914043E-5	9.89337066431864E-4	0.0	0.04514733702261836	0.0
42-43	1.766673332914043E-5	9.981704330964342E-4	0.0	0.05621554545332484	0.0
44-45	1.766673332914043E-5	0.0011218375664004171	0.0	0.06867059245036886	0.0
46-47	1.766673332914043E-5	0.0012543380663689705	0.0	0.08071930458084262	0.0
48-49	1.766673332914043E-5	0.0012543380663689705	0.0	0.09272384987799354	0.0
50-51	1.766673332914043E-5	0.001484005599647796	0.0	0.10423372664192852	0.0
52-53	1.766673332914043E-5	0.0015016723329769365	0.0	0.12903782023604168	0.0
54-55	1.766673332914043E-5	0.0015105056996415067	0.0	0.15764026149592003	0.0
56-57	1.766673332914043E-5	0.0015370057996352173	0.0	0.18686103842231833	0.0
58-59	1.766673332914043E-5	0.0015370057996352173	0.0	0.20788445108399542	0.0
60-61	1.766673332914043E-5	0.0015370057996352173	0.0	0.223554843546943	0.0
62-63	1.766673332914043E-5	0.001563505899628928	0.0	0.2356742226107333	0.0
64-65	1.766673332914043E-5	0.0017666733329140428	0.0	0.24065624140955091	0.0
66-67	1.766673332914043E-5	0.0017843400662431834	0.0	0.2458855944749765	0.0
68-69	1.766673332914043E-5	0.0018285068995660344	0.0	0.2511326142737312	0.0
70-71	2.6500099993710645E-5	0.0018726737328888855	0.0	0.25622063347252366	0.0
72-73	3.533346665828086E-5	0.0018726737328888855	0.0	0.2601956484715803	0.0
74-75	3.533346665828086E-5	0.0019256739328763067	0.0	0.26322549323752786	0.0
76-77	3.533346665828086E-5	0.0020316743328511492	0.0	0.2660345038368611	0.0
78-79	3.533346665828086E-5	0.002102341266167711	0.0	0.26796901113640204	0.0
80-81	3.533346665828086E-5	0.002102341266167711	0.0	0.26845484630295335	0.0
82-83	3.533346665828086E-5	0.002111174632832281	0.0	0.26888768126951734	0.0
84-85	3.533346665828086E-5	0.0021288413661614217	0.0	0.2692056824694419	0.0
86-87	3.533346665828086E-5	0.002155341466155132	0.0	0.26925868266942926	0.0
88	3.533346665828086E-5	0.0022790085994591155	0.0	0.26925868266942926	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	10865	0.0	54.104584	1
GGTATCA	7355	0.0	48.81423	1
TATACAC	14015	0.0	43.53766	3
TTATACA	13605	0.0	43.48687	2
GTATCAA	12985	0.0	35.73016	1
TACACAT	19575	0.0	30.950392	5
TCAACGC	15505	0.0	29.325083	4
ATCAACG	15550	0.0	29.131823	3
CAACGCA	16000	0.0	28.307238	5
TATCAAC	16195	0.0	28.205767	2
ATACACA	22235	0.0	28.162502	4
ACACATC	21105	0.0	27.947815	6
AACGCAG	16565	0.0	27.494093	6
ACATCTC	22450	0.0	26.254667	8
CACATCT	23130	0.0	25.246012	7
ACGCAGA	18790	0.0	24.103874	7
CGCAGAG	19120	0.0	23.70989	8
CATCTCC	26035	0.0	22.526142	9
GTGGTAT	3990	0.0	21.967468	1
TGGTATC	3815	0.0	20.655542	2
>>END_MODULE