FastQCFastQC Report
Fri 27 May 2016
SRR2138666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5626841
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62300.048.5695231
GTATCAA117200.037.593491
ATCAACG139750.030.883383
TCAACGC140250.030.7424474
CAACGCA141450.030.4773085
TATCAAC147500.029.7837262
AACGCAG147350.029.3439626
ACGCAGA167700.025.706487
CGCAGAG169250.025.471068
GCAGAGT202800.021.0882389
GTACATG148200.019.405351
AGAGTAC183200.018.80651310-11
GAGTACT121050.018.7978212-13
TACATGG150100.018.6171862
CAGAGTA197650.018.24462910-11
ACATGGG148800.018.2037683
GTACTTT138550.016.42393714-15
TATACCG3403.5124685E-916.3849835
AGTACTT138350.016.137512-13
GTGGTAT35750.015.9449361
Produced by FastQC (version 0.11.5)