FastQCFastQC Report
Fri 27 May 2016
SRR2138665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6314967
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74800.050.642011
GTATCAA120000.032.2377361
CGAGACC46050.027.91474786-87
AGACCGA46550.027.91008688-89
GACCGAG46950.027.41689190-91
GAGACCG46550.027.12132388-89
ACCGAGG48950.026.76627590-91
TCAACGC141050.026.6693085
ACGAGAC47650.026.56778786-87
ATCAACG143400.026.3271334
CAACGCA144350.026.0302726
CCCACGA49150.025.85405282-83
CACGAGA51300.025.12497784-85
AACGCAG150650.024.9698377
TATCAAC153050.024.9163363
TCTCCGA49250.024.72767874-75
GCCCACG50050.024.6823882-83
ATCTCCG48400.024.51538774-75
TCCGAGC50450.024.39143476-77
CGAGCCC55550.022.62424778-79
Produced by FastQC (version 0.11.5)