##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138665_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6314967
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83214179899911	33.0	33.0	33.0	33.0	33.0
2	32.824837564471835	33.0	33.0	33.0	33.0	33.0
3	32.83024788569758	33.0	33.0	33.0	33.0	33.0
4	36.78087818986228	37.0	37.0	37.0	37.0	37.0
5	36.79199131206862	37.0	37.0	37.0	37.0	37.0
6	36.80156222510743	37.0	37.0	37.0	37.0	37.0
7	36.78917102813047	37.0	37.0	37.0	37.0	37.0
8	36.78341201149586	37.0	37.0	37.0	37.0	37.0
9	36.78286394845769	37.0	37.0	37.0	37.0	37.0
10-11	36.75983294924582	37.0	37.0	37.0	37.0	37.0
12-13	36.74429692189999	37.0	37.0	37.0	37.0	37.0
14-15	39.10307251011763	40.0	40.0	40.0	37.0	40.0
16-17	39.05811091332703	40.0	40.0	40.0	37.0	40.0
18-19	39.06901081193298	40.0	40.0	40.0	37.0	40.0
20-21	39.10350053202167	40.0	40.0	40.0	37.0	40.0
22-23	39.12375695820279	40.0	40.0	40.0	37.0	40.0
24-25	39.039387989971104	40.0	40.0	40.0	37.0	40.0
26-27	38.962775089614624	40.0	40.0	40.0	37.0	40.0
28-29	38.9380498670111	40.0	40.0	40.0	37.0	40.0
30-31	38.89939030700751	40.0	40.0	40.0	37.0	40.0
32-33	38.7560214815589	40.0	40.0	40.0	37.0	40.0
34-35	38.83635151590936	40.0	40.0	40.0	37.0	40.0
36-37	38.52904895249789	40.0	37.0	40.0	37.0	40.0
38-39	38.71350686425356	40.0	37.0	40.0	37.0	40.0
40-41	38.719326109581985	40.0	37.0	40.0	37.0	40.0
42-43	38.66528387033326	40.0	37.0	40.0	37.0	40.0
44-45	38.58917640723293	40.0	37.0	40.0	37.0	40.0
46-47	38.5160413678802	40.0	37.0	40.0	37.0	40.0
48-49	38.467616357535235	40.0	37.0	40.0	37.0	40.0
50-51	38.409756060137454	40.0	37.0	40.0	37.0	40.0
52-53	38.348985342236645	40.0	37.0	40.0	37.0	40.0
54-55	38.26732516032475	40.0	37.0	40.0	37.0	40.0
56-57	38.14658420039888	40.0	37.0	40.0	37.0	40.0
58-59	38.01451463798737	40.0	37.0	40.0	37.0	40.0
60-61	37.85810687898304	40.0	37.0	40.0	33.0	40.0
62-63	37.66291770750458	38.5	37.0	40.0	33.0	40.0
64-65	37.44132293984808	37.0	37.0	40.0	33.0	40.0
66-67	37.202799478066105	37.0	37.0	40.0	33.0	40.0
68-69	36.94178501191471	37.0	37.0	40.0	33.0	40.0
70-71	36.68098916817081	37.0	37.0	40.0	33.0	40.0
72-73	36.423013530474186	37.0	37.0	37.0	33.0	40.0
74-75	36.170043579822135	37.0	37.0	37.0	33.0	40.0
76-77	34.50007661278466	35.0	33.0	37.0	30.0	37.0
78-79	35.7826160363409	37.0	35.0	37.0	33.0	37.0
80-81	35.89238450466647	37.0	37.0	37.0	33.0	37.0
82-83	35.810035919101495	37.0	37.0	37.0	33.0	37.0
84-85	35.69234475229405	37.0	37.0	37.0	33.0	37.0
86-87	35.603469033471114	37.0	37.0	37.0	33.0	37.0
88-89	35.51949093365948	37.0	37.0	37.0	33.0	37.0
90-91	35.446825443993475	37.0	33.0	37.0	33.0	37.0
92-93	35.3795293819758	37.0	33.0	37.0	33.0	37.0
94-95	35.326617872719076	37.0	33.0	37.0	33.0	37.0
96-97	35.28678515735599	37.0	33.0	37.0	33.0	37.0
98-99	35.25058060463019	37.0	33.0	37.0	33.0	37.0
100	35.16018394993332	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	18.0
21	69.0
22	320.0
23	967.0
24	2433.0
25	5209.0
26	9694.0
27	15991.0
28	24433.0
29	35149.0
30	48847.0
31	66641.0
32	90128.0
33	123951.0
34	177917.0
35	278314.0
36	555485.0
37	1749898.0
38	2993473.0
39	136028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59640628367496	19.691488490755376	13.226846632769417	24.485258592800246
2	15.144180484236895	20.571857303450674	40.838693218824424	23.44526899348801
3	18.615292257812886	24.9401694811384	30.200520323215446	26.24401793783327
4	12.484262519780245	16.88899891332392	38.40445668651066	32.222281880385175
5	13.327310559753727	37.52761713529229	35.70631140220304	13.438760902750946
6	31.188888239637674	37.17311586901405	18.079207698155823	13.55878819319246
7	26.985065796860063	32.318712037608435	22.744046010058327	17.95217615547318
8	24.75184747600423	36.660809153872066	20.940125261145464	17.64721810897824
9	25.596459965665698	16.168572218983883	21.18031654005476	37.054651275295655
10-11	24.545852733672245	26.47551444053469	28.66779826402893	20.31083456176414
12-13	24.893122956937066	24.05459917684447	28.93814805366362	22.11412981255484
14-15	21.976765674309938	25.506126952048998	26.457454171969548	26.059653201671523
16-17	21.803075138793282	28.57356182542205	27.352541984779965	22.2708210510047
18-19	21.82517343321034	27.852014111871053	28.870918882078083	21.451893572840522
20-21	22.46295074013007	27.13759312197498	28.849292364837343	21.550163773057605
22-23	22.22555144048693	27.08374838584823	28.664880713511305	22.025819460153535
24-25	22.05722481098806	27.11420995631052	28.781414933499512	22.047150299201913
26-27	21.98624517716878	27.32960809193385	28.71029771310674	21.973849017790627
28-29	21.93290042173772	27.43500871363846	28.71762138497065	21.914469479653167
30-31	22.15078672280224	27.28843758795021	28.630922272000088	21.929853417247465
32-33	21.95128632461243	27.434634629915756	28.598028764497535	22.01605028097428
34-35	22.11488332612732	27.428230569870177	28.592369381837706	21.864516722164797
36-37	22.164085159555373	27.41355718238377	28.45998417834295	21.962373479717908
38-39	21.943106344003436	27.526456410606563	28.601649462710533	21.92878778267947
40-41	22.208538898550497	27.424237208282946	28.447939171820902	21.91928472134565
42-43	22.049890610902345	27.52143612287489	28.37125639025406	22.05741687596871
44-45	22.147693970506463	27.633340520665566	28.175963427148282	22.04300208167969
46-47	22.173200071277606	27.59967148141381	27.940512438535386	22.286616008773194
48-49	22.167975464024895	27.685693261337878	27.928281014760337	22.218050259876886
50-51	22.086247269773875	27.82928712424466	27.871547590178853	22.212918015802604
52-53	22.257839777450428	27.782167775385325	27.696550668641294	22.263441778522957
54-55	22.269155863752623	27.690504532995377	27.758100391220648	22.282239212031353
56-57	22.22609521526601	27.739955034899683	27.74962879455185	22.284320955282457
58-59	22.162137659913764	27.770083826441677	27.71878984145424	22.348988672190323
60-61	22.385103053159547	27.63952062832769	27.86767235338336	22.1077039651294
62-63	22.122681658899623	27.743331805365802	27.81251436653992	22.321472169194656
64-65	22.099768221719007	27.73454335680293	27.767862948056198	22.39782547342186
66-67	22.13997059231264	27.800554973512625	27.83240799870915	22.22706643546558
68-69	22.137009649236685	27.94698896771034	27.70665321562571	22.20934816742727
70-71	22.134552149710693	27.752616578249555	27.523170426132566	22.589660845907183
72-73	22.168601989740377	27.778195043704912	27.505399899756284	22.547803066798426
74-75	22.07764877785366	27.945134822189598	27.668167548136736	22.309048851820005
76-77	22.20697381826884	27.70344088085756	27.571519870113242	22.518065430760362
78-79	22.177569438803832	27.70079082928245	27.297335603774652	22.824304128139065
80-81	22.336545943815192	27.95750931028128	27.348954297673345	22.35699044823018
82-83	22.387020718963488	27.716929732682118	27.440341747254955	22.455707801099443
84-85	22.31631962402833	27.65486798237797	27.382917158180486	22.64589523541321
86-87	22.16305122204823	27.92224438554679	27.417509673512974	22.497194718892004
88-89	22.41402668164236	27.939858279588858	27.426543690902683	22.2195713478661
90-91	22.41495060243858	27.999232978161437	27.371377561778004	22.214438857621982
92-93	22.244825545784717	27.70561834994122	27.447743740028645	22.60181236424542
94-95	22.340509729802662	28.012163647856646	27.487589757579546	22.159736864761143
96-97	22.68937933641505	27.82522054816508	27.55505748385118	21.930342631568685
98-99	23.442171874703195	29.835424732753545	27.90875868218121	18.813644710362052
100	22.309232467485813	30.850690239915462	21.765975078586592	25.07410221401213
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1791.0
1	1504.5
2	1712.0
3	2411.0
4	3193.5
5	4866.0
6	6411.0
7	7701.5
8	8655.5
9	8696.0
10	8041.0
11	7457.5
12	8104.0
13	10342.0
14	15474.0
15	22201.0
16	27375.0
17	30167.5
18	31096.5
19	30677.5
20	29425.0
21	28483.0
22	29059.0
23	32076.0
24	37603.5
25	45783.5
26	56317.5
27	67358.5
28	77752.5
29	89293.0
30	101325.0
31	112717.0
32	125775.0
33	139297.5
34	152520.5
35	166039.0
36	180227.0
37	193479.5
38	203266.0
39	212208.0
40	222242.0
41	231544.0
42	238695.5
43	244830.5
44	250889.0
45	256906.0
46	262162.0
47	265327.5
48	266545.0
49	266171.0
50	263210.0
51	256168.0
52	246124.5
53	234230.5
54	221418.5
55	208652.5
56	193788.0
57	176630.5
58	159323.0
59	140152.5
60	118183.5
61	96915.5
62	77939.5
63	61101.5
64	46312.5
65	34438.5
66	25966.5
67	19993.0
68	15278.5
69	11238.5
70	8226.0
71	6001.0
72	4284.5
73	2990.5
74	2058.5
75	1414.5
76	971.5
77	619.0
78	380.5
79	247.5
80	183.0
81	143.0
82	104.5
83	65.0
84	40.0
85	26.5
86	20.5
87	14.5
88	8.5
89	6.5
90	6.0
91	5.5
92	4.5
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	7.28428192894753E-4
4	0.019287511716213245
5	7.442635883924651E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.007420689500056982
26-27	0.05128617471601093
28-29	0.08609027667401394
30-31	0.07649933334026737
32-33	0.035216382941547095
34-35	0.004313036688527107
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.334762582748958E-4
56-57	6.367718285164743E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	4.6367724057884353E-5
72-73	0.013296632131629916
74-75	0.02914694711246752
76-77	0.0037305938752426435
78-79	0.0
80-81	0.0
82-83	0.01090886055230115
84-85	0.05079970034833513
86-87	0.041643899193860835
88-89	0.010715104349997518
90-91	1.5397898638507538E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	80.0
20-21	297.0
22-23	675.0
24-25	1767.0
26-27	3182.0
28-29	7774.0
30-31	15628.0
32-33	19598.0
34-35	27106.0
36-37	40205.0
38-39	43970.0
40-41	44263.0
42-43	50630.0
44-45	59526.0
46-47	59351.0
48-49	54308.0
50-51	47290.0
52-53	45088.0
54-55	49417.0
56-57	51575.0
58-59	29243.0
60-61	50742.0
62-63	48640.0
64-65	50867.0
66-67	54245.0
68-69	54531.0
70-71	52740.0
72-73	49603.0
74-75	49857.0
76-77	50263.0
78-79	53241.0
80-81	53456.0
82-83	50798.0
84-85	50426.0
86-87	53138.0
88-89	56073.0
90-91	58470.0
92-93	59940.0
94-95	70567.0
96-97	221747.0
98-99	382667.0
100-101	4091983.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.37469888965667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.89675533883118	79.42076627777166
2	6.707463338933857	11.721251791055137
3	1.4157322933504677	3.710975485195806
4	0.47400698136485797	1.6566486907339826
5	0.2010821124677825	0.8784744514484286
6	0.1119326541241684	0.586804917001158
7	0.057044904593301235	0.34889969534202214
8	0.035863632794382955	0.25068592931987416
9	0.023939734369988572	0.18825543738685044
>10	0.07372651398777051	0.9952425686258408
>50	0.002019942812598577	0.11842776503840335
>100	3.7985078712777945E-4	0.07940710383976518
>500	2.6350791289915485E-5	0.019938718655628393
>1k	2.635079125127782E-5	0.02422116858540998
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.167079099542405E-5	0.0	0.0	0.0
3	0.0	3.167079099542405E-5	0.0	0.0	0.0
4	0.0	4.750618649313607E-5	0.0	1.5835395497712024E-5	0.0
5	0.0	4.750618649313607E-5	0.0	1.5835395497712024E-5	0.0
6	1.266831639816962E-4	4.750618649313607E-5	0.0	1.5835395497712024E-5	0.0
7	1.266831639816962E-4	4.750618649313607E-5	0.0	3.167079099542405E-5	0.0
8	1.266831639816962E-4	4.750618649313607E-5	0.0	3.167079099542405E-5	0.0
9	1.266831639816962E-4	9.501237298627214E-5	0.0	4.750618649313607E-5	0.0
10-11	1.266831639816962E-4	9.501237298627214E-5	0.0	8.709467523741613E-5	0.0
12-13	1.266831639816962E-4	9.501237298627214E-5	0.0	1.1084776848398416E-4	0.0
14-15	1.266831639816962E-4	1.504362572282642E-4	0.0	1.5835395497712022E-4	2.3753093246568034E-5
16-17	1.7418935047483226E-4	1.5835395497712022E-4	0.0	1.5835395497712022E-4	3.167079099542405E-5
18-19	1.9002474597254427E-4	1.5835395497712022E-4	0.0	1.7418935047483226E-4	3.958848874428006E-5
20-21	1.9002474597254427E-4	1.5835395497712022E-4	0.0	2.2169553696796832E-4	4.750618649313607E-5
22-23	1.9002474597254427E-4	1.9002474597254427E-4	0.0	2.2169553696796832E-4	4.750618649313607E-5
24-25	1.9002474597254427E-4	2.1377783921911232E-4	0.0	2.929548167076724E-4	4.750618649313607E-5
26-27	1.9002474597254427E-4	2.2169553696796832E-4	0.0	4.1172028294051257E-4	4.750618649313607E-5
28-29	1.9002474597254427E-4	2.2169553696796832E-4	0.0	0.0011876546623284018	4.750618649313607E-5
30-31	1.9002474597254427E-4	2.3753093246568034E-4	0.0	0.0036817294532180455	4.750618649313607E-5
32-33	1.9002474597254427E-4	2.3753093246568034E-4	0.0	0.007410965092929227	4.750618649313607E-5
34-35	2.058601414702563E-4	3.246256077030965E-4	0.0	0.011781534250297744	4.750618649313607E-5
36-37	2.058601414702563E-4	4.117202829405126E-4	0.0	0.01919249934322697	4.750618649313607E-5
38-39	2.058601414702563E-4	4.2755567843822463E-4	0.0	0.03593843008205744	4.750618649313607E-5
40-41	2.058601414702563E-4	4.5922646943364866E-4	0.0	0.053982863251700285	4.750618649313607E-5
42-43	2.2169553696796832E-4	4.5922646943364866E-4	0.0	0.06543185419654608	5.542388424199208E-5
44-45	2.2169553696796832E-4	4.7506186493136073E-4	0.0	0.08004000654318541	6.33415819908481E-5
46-47	2.2169553696796832E-4	5.067326559267848E-4	0.0	0.09458481730783391	6.33415819908481E-5
48-49	2.2169553696796832E-4	5.067326559267848E-4	0.0	0.1084803768570762	7.917697748856011E-5
50-51	2.4544863021453637E-4	5.700742379176328E-4	0.0	0.12279557438700789	7.917697748856011E-5
52-53	2.533663279633924E-4	5.700742379176328E-4	0.0	0.15251861173621334	7.917697748856011E-5
54-55	2.533663279633924E-4	5.938273311642008E-4	0.0	0.18961302568960375	7.917697748856011E-5
56-57	2.533663279633924E-4	6.175804244107689E-4	0.0	0.22828306149501654	7.917697748856011E-5
58-59	2.533663279633924E-4	6.33415819908481E-4	0.0	0.4329080421164513	7.917697748856011E-5
60-61	2.533663279633924E-4	6.65086610903905E-4	0.0	0.622251866082594	7.917697748856011E-5
62-63	2.850371189588164E-4	6.80922006401617E-4	0.0	0.6293065347768246	7.917697748856011E-5
64-65	2.850371189588164E-4	7.759343793878891E-4	0.0	0.6360920017475942	7.917697748856011E-5
66-67	3.1670790995424043E-4	7.759343793878891E-4	0.0	0.642647855483647	7.917697748856011E-5
68-69	3.1670790995424043E-4	7.838520771367452E-4	0.0	0.6492987215926861	7.917697748856011E-5
70-71	3.4046100320080845E-4	7.917697748856011E-4	0.0	0.6548490277146342	7.917697748856011E-5
72-73	4.117202829405126E-4	7.917697748856011E-4	0.0	0.6594412924089705	1.1084776848398416E-4
74-75	4.117202829405126E-4	8.313582636298812E-4	0.0	0.6627825608589879	1.1084776848398416E-4
76-77	4.117202829405126E-4	8.551113568764493E-4	0.0	0.6660446523315166	1.1084776848398416E-4
78-79	4.117202829405126E-4	8.551113568764493E-4	0.0	0.6680399121642282	1.1084776848398416E-4
80-81	4.117202829405126E-4	8.709467523741613E-4	0.0	0.6686654102863878	1.1876546623284018E-4
82-83	4.117202829405126E-4	8.709467523741613E-4	0.0	0.6691642252445658	1.266831639816962E-4
84-85	4.117202829405126E-4	8.709467523741613E-4	0.0	0.669322579199543	1.425185594794082E-4
86-87	4.117202829405126E-4	8.867821478718733E-4	0.0	0.669370085386036	1.5835395497712022E-4
88	4.117202829405126E-4	9.659591253604334E-4	0.0	0.6693780030837849	1.5835395497712022E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7480	0.0	50.64201	1
GTATCAA	12000	0.0	32.237736	1
CGAGACC	4605	0.0	27.914747	86-87
AGACCGA	4655	0.0	27.910086	88-89
GACCGAG	4695	0.0	27.416891	90-91
GAGACCG	4655	0.0	27.121323	88-89
ACCGAGG	4895	0.0	26.766275	90-91
TCAACGC	14105	0.0	26.669308	5
ACGAGAC	4765	0.0	26.567787	86-87
ATCAACG	14340	0.0	26.327133	4
CAACGCA	14435	0.0	26.030272	6
CCCACGA	4915	0.0	25.854052	82-83
CACGAGA	5130	0.0	25.124977	84-85
AACGCAG	15065	0.0	24.969837	7
TATCAAC	15305	0.0	24.916336	3
TCTCCGA	4925	0.0	24.727678	74-75
GCCCACG	5005	0.0	24.68238	82-83
ATCTCCG	4840	0.0	24.515387	74-75
TCCGAGC	5045	0.0	24.391434	76-77
CGAGCCC	5555	0.0	22.624247	78-79
>>END_MODULE