FastQCFastQC Report
Fri 27 May 2016
SRR2138664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5460945
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73200.046.756451
GTATCAA130750.035.9433021
TCAACGC150100.030.6216494
ATCAACG150500.030.512253
CAACGCA152750.030.1378695
AACGCAG159350.028.943036
TATCAAC162000.028.8505462
ACGCAGA183600.025.0733957
CGCAGAG189200.024.3312638
GCAGAGT219200.021.0600819
GTACATG164800.020.7419431
TACATGG163050.020.226192
GAGTACT120350.019.9289512-13
ACATGGG166650.019.247443
GTGGTAT42500.019.2222481
CAGAGTA207200.018.99174710-11
AGAGTAC190500.018.33262610-11
GTACTTT131100.018.32792914-15
TGGTATC41400.018.0712592
CATGGGG119300.017.6958984
Produced by FastQC (version 0.11.5)