FastQCFastQC Report
Fri 27 May 2016
SRR2138663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5997366
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89400.049.342921
GTATCAA157500.034.4797251
ATCAACG180900.028.9542963
TCAACGC182200.028.7474654
CAACGCA185900.028.263215
TATCAAC190800.027.8110942
AACGCAG191150.027.5312966
ACGCAGA219200.023.9691737
CGCAGAG222500.023.6136788
GTGGTAT43100.022.0597611
TGGTATC43300.020.8698672
GCAGAGT256300.020.4494639
GAGTACT143400.020.12610812-13
TACATGG181900.020.0365052
GTACATG187300.019.9618871
ACATGGG185700.019.1883013
CAGAGTA254600.018.86209710-11
AGAGTAC229900.018.18789110-11
GTACTTT160550.017.79027614-15
CATGGGG138650.016.8961964
Produced by FastQC (version 0.11.5)