##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138663_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5997366
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84089965494852	33.0	33.0	33.0	33.0	33.0
2	32.837020618718284	33.0	33.0	33.0	33.0	33.0
3	32.835974993021935	33.0	33.0	33.0	33.0	33.0
4	36.79118999907626	37.0	37.0	37.0	37.0	37.0
5	36.80100647517594	37.0	37.0	37.0	37.0	37.0
6	36.81033573738871	37.0	37.0	37.0	37.0	37.0
7	36.800866413688944	37.0	37.0	37.0	37.0	37.0
8	36.79566913208232	37.0	37.0	37.0	37.0	37.0
9	36.79082700638914	37.0	37.0	37.0	37.0	37.0
10-11	36.76999861272432	37.0	37.0	37.0	37.0	37.0
12-13	36.75713579928255	37.0	37.0	37.0	37.0	37.0
14-15	39.12397459151234	40.0	40.0	40.0	37.0	40.0
16-17	39.08510511114379	40.0	40.0	40.0	37.0	40.0
18-19	39.10760015313389	40.0	40.0	40.0	37.0	40.0
20-21	39.14311069464155	40.0	40.0	40.0	37.0	40.0
22-23	39.164161208187	40.0	40.0	40.0	37.0	40.0
24-25	39.07728148375904	40.0	40.0	40.0	37.0	40.0
26-27	39.00199551926396	40.0	40.0	40.0	37.0	40.0
28-29	38.977807458372084	40.0	40.0	40.0	37.0	40.0
30-31	38.93368840013051	40.0	40.0	40.0	37.0	40.0
32-33	38.78758700807019	40.0	40.0	40.0	37.0	40.0
34-35	38.86813131884237	40.0	40.0	40.0	37.0	40.0
36-37	38.5628491558097	40.0	37.0	40.0	37.0	40.0
38-39	38.74308830310566	40.0	37.0	40.0	37.0	40.0
40-41	38.75192682242173	40.0	37.0	40.0	37.0	40.0
42-43	38.70140228545601	40.0	37.0	40.0	37.0	40.0
44-45	38.61972254153676	40.0	37.0	40.0	37.0	40.0
46-47	38.547483382095834	40.0	37.0	40.0	37.0	40.0
48-49	38.501132477010955	40.0	37.0	40.0	37.0	40.0
50-51	38.43847641740389	40.0	37.0	40.0	37.0	40.0
52-53	38.37776495087264	40.0	37.0	40.0	37.0	40.0
54-55	38.29878761577625	40.0	37.0	40.0	37.0	40.0
56-57	38.17884623813063	40.0	37.0	40.0	37.0	40.0
58-59	38.04785569651713	40.0	37.0	40.0	37.0	40.0
60-61	37.89241560629124	40.0	37.0	40.0	35.0	40.0
62-63	37.702866115845595	38.5	37.0	40.0	33.0	40.0
64-65	37.483866312095344	37.0	37.0	40.0	33.0	40.0
66-67	37.244509889513694	37.0	37.0	40.0	33.0	40.0
68-69	36.99097248864785	37.0	37.0	40.0	33.0	40.0
70-71	36.73974578684727	37.0	37.0	40.0	33.0	40.0
72-73	36.487779933230584	37.0	37.0	38.5	33.0	40.0
74-75	36.23515975366018	37.0	37.0	37.0	33.0	40.0
76-77	34.56702637645783	35.0	33.0	37.0	30.0	37.0
78-79	35.8356700469687	37.0	37.0	37.0	33.0	37.0
80-81	35.94298102435983	37.0	37.0	37.0	33.0	37.0
82-83	35.85878437754009	37.0	37.0	37.0	33.0	37.0
84-85	35.74294146115582	37.0	37.0	37.0	33.0	37.0
86-87	35.65736834912628	37.0	37.0	37.0	33.0	37.0
88-89	35.576557135205434	37.0	37.0	37.0	33.0	37.0
90-91	35.50698785706436	37.0	35.0	37.0	33.0	37.0
92-93	35.44128490260805	37.0	33.0	37.0	33.0	37.0
94-95	35.396007770017405	37.0	33.0	37.0	33.0	37.0
96-97	35.353946047325074	37.0	33.0	37.0	33.0	37.0
98-99	35.311369477838824	37.0	33.0	37.0	33.0	37.0
100	35.219640818057734	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	17.0
21	86.0
22	301.0
23	949.0
24	2382.0
25	5004.0
26	9124.0
27	15080.0
28	22733.0
29	32496.0
30	44247.0
31	60680.0
32	81789.0
33	111931.0
34	160542.0
35	254528.0
36	515957.0
37	1669917.0
38	2876673.0
39	132926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.573350700957725	20.242253015740577	14.645979585037832	23.53841669826387
2	14.560358664120216	20.71744495833671	42.00719115691789	22.715005220625187
3	18.122043057189156	25.975076225637896	30.52291971455279	25.379961002620167
4	12.435740305864886	18.076267595169387	39.028350897580246	30.459641201385484
5	13.391072562711043	37.716432486636926	35.42360415312935	13.46889079752269
6	29.754595600802087	37.851233358110875	18.85512740092901	13.53904364015803
7	26.536849677008206	32.8748653992436	22.842627913654095	17.7456570100941
8	24.38610550031464	36.6906405245236	21.018443763478835	17.90481021168293
9	25.46859738091689	17.3516006860345	21.34206916836491	35.83773276468369
10-11	24.190719725959696	26.905052984927046	28.69072856317257	20.21349872594069
12-13	25.067287872709453	24.36943985076115	28.874442546944774	21.688829729584622
14-15	22.184597371579457	25.81826755278901	26.67578566990909	25.321349405722444
16-17	21.637440503047507	29.04582611766566	27.28474466957661	22.03198870971023
18-19	21.653139061381278	28.130232505403207	29.070461932788493	21.14616650042702
20-21	22.5550159756342	27.16785737748818	29.03320150642177	21.243925140455854
22-23	22.283418946028775	27.17500283272703	28.84947593090831	21.692102290335885
24-25	22.100930699817077	27.29949105819964	28.921789957969313	21.677788284013978
26-27	21.97732102465083	27.496490393053445	28.87304248808912	21.653146094206605
28-29	21.932460160315134	27.532806634121403	28.873279377082195	21.661453828481267
30-31	22.140027257244192	27.409741626373428	28.858826754638066	21.591404361744313
32-33	22.004573208387793	27.54006121001698	28.74650932163629	21.708856259958935
34-35	22.140463225195752	27.486069192955327	28.76441079260167	21.609056789247248
36-37	22.120455800563875	27.52243639563543	28.617581035353567	21.739526768447128
38-39	21.924391966789383	27.523860667371213	28.780276745547162	21.77147062029224
40-41	22.16262258616363	27.47346724382535	28.597006564336684	21.766903605674337
42-43	22.09135454221986	27.48970921733464	28.52591236651562	21.893023873929874
44-45	22.17801872366755	27.66288584024262	28.28572626044711	21.873369175642726
46-47	22.249155512572365	27.597077356225665	27.91143989957282	22.242327231629154
48-49	22.166279315481923	27.684217027159274	27.957403862039985	22.19209979531881
50-51	22.12481999009447	27.79852119235937	27.88805400979878	22.18860480774738
52-53	22.233790086914336	27.83027362270613	27.779249429203105	22.15668686117643
54-55	22.299575505973614	27.760393468398686	27.678927369929813	22.261103655697887
56-57	22.36730656039062	27.858119882396604	27.59038803901556	22.18418551819721
58-59	22.212165828671086	27.98003024347698	27.753076279003658	22.054727648848278
60-61	22.35714396894972	27.972669918923227	27.601340899602274	22.068845212524778
62-63	22.279650918947357	28.076318791755934	27.51409324359043	22.12993704570628
64-65	22.248288446265573	27.957890132098484	27.56949351043093	22.22432791120501
66-67	22.334257975558906	27.98113019274992	27.4151663751253	22.26944545656588
68-69	22.308527617450906	27.88237338798204	27.50885109893276	22.3002478956343
70-71	22.312744789772267	27.65204765122162	27.571735521818002	22.46347203718812
72-73	22.3791173558308	27.712318186556097	27.511786563555546	22.39677789405756
74-75	22.265699913577063	27.93390384818727	27.443755759120002	22.356640479115665
76-77	22.365312474426794	27.833066416604368	27.37042612718783	22.431194981781005
78-79	22.33991671199259	27.85673497368304	27.2837035897933	22.51964472453107
80-81	22.32862403854751	27.82919150595136	27.392122795274354	22.450061660226776
82-83	22.395479270125566	27.7907696274595	27.471346362554357	22.342404739860577
84-85	22.5519355776033	27.802909193210716	27.340399426252148	22.304755802933833
86-87	22.31573500445623	27.80033016950263	27.422312417968136	22.461622408073005
88-89	22.437249357584854	27.78511464639527	27.412302375679687	22.365333620340184
90-91	22.45228765032727	27.912960328144194	27.384865966365474	22.249886055163064
92-93	22.505392980814868	27.834581129450385	27.388053883545883	22.271972006188864
94-95	22.30879952378448	27.94774640656249	27.41687236350684	22.326581706146182
96-97	22.369602652097463	27.99779595042301	27.587946002550723	22.04465539492881
98-99	23.52978374045705	29.875468273605655	27.70456866091944	18.890179325017854
100	22.5003138565543	30.811810609701993	21.548015551279658	25.139859982464046
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1553.0
1	1321.0
2	1493.0
3	2054.0
4	2834.0
5	4512.5
6	5868.0
7	6769.5
8	7462.5
9	7509.5
10	7110.0
11	6818.5
12	7690.0
13	10312.0
14	16063.0
15	23614.5
16	29945.5
17	34049.5
18	35066.0
19	33177.0
20	30099.5
21	27001.5
22	25427.0
23	27082.5
24	31952.0
25	39707.0
26	50168.0
27	62371.5
28	74620.5
29	88315.0
30	100913.0
31	112468.5
32	126370.0
33	140606.0
34	154494.5
35	167341.5
36	181501.5
37	194103.5
38	200951.5
39	206444.5
40	215147.5
41	222352.0
42	226112.0
43	230913.5
44	236806.0
45	243060.0
46	248000.5
47	251291.5
48	250934.5
49	247027.5
50	241829.5
51	234006.5
52	223040.5
53	210350.5
54	197965.5
55	183953.5
56	168547.0
57	152789.0
58	136941.5
59	121069.5
60	101599.0
61	81418.0
62	64089.5
63	49068.5
64	36543.5
65	27151.0
66	20661.5
67	16034.0
68	12131.5
69	8828.5
70	6484.5
71	4767.0
72	3400.5
73	2352.5
74	1659.5
75	1107.5
76	689.0
77	443.5
78	278.0
79	165.5
80	101.0
81	68.5
82	48.5
83	31.0
84	21.0
85	15.5
86	11.5
87	9.5
88	9.0
89	7.5
90	4.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.669594618704277E-4
4	0.019825370004098467
5	9.504172331653595E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.337142712791186E-6
22-23	0.0
24-25	0.007772046217990552
26-27	0.05091667108072386
28-29	0.0852927222023549
30-31	0.07498851487735878
32-33	0.03523245383619395
34-35	0.004364268358525653
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.697247943977801E-4
56-57	7.973539446911813E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	6.739205549716515E-5
72-73	0.014394472056512581
74-75	0.029458806360907704
76-77	0.003538119282058276
78-79	0.0
80-81	0.0
82-83	0.01113221091503622
84-85	0.05035724705643059
86-87	0.04104183894792805
88-89	0.01037430513877753
90-91	8.467793066613376E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	63.0
20-21	290.0
22-23	697.0
24-25	1736.0
26-27	2458.0
28-29	4673.0
30-31	11010.0
32-33	14508.0
34-35	20348.0
36-37	29234.0
38-39	34040.0
40-41	36600.0
42-43	43128.0
44-45	51885.0
46-47	51417.0
48-49	45464.0
50-51	40166.0
52-53	38326.0
54-55	41732.0
56-57	44796.0
58-59	45013.0
60-61	43850.0
62-63	44444.0
64-65	47275.0
66-67	50097.0
68-69	48859.0
70-71	46470.0
72-73	44169.0
74-75	44662.0
76-77	44521.0
78-79	47487.0
80-81	48193.0
82-83	46099.0
84-85	46201.0
86-87	48445.0
88-89	51557.0
90-91	54615.0
92-93	56216.0
94-95	66421.0
96-97	191360.0
98-99	370199.0
100-101	3998642.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.22112409557619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.49210482150902	76.2661777056813
2	7.452929952571835	12.70294136727522
3	1.7516729346067283	4.478386096049464
4	0.6318601589877924	2.153913320805966
5	0.2844578089552401	1.2120907118465107
6	0.1368123588605613	0.6995581807358638
7	0.08057088972519001	0.4806439254232982
8	0.04803220283464113	0.32746866546838704
9	0.03021451458943559	0.23174234075824984
>10	0.08904587523939211	1.129248959621069
>50	0.0013378187062254938	0.07579647381542061
>100	8.691805836259269E-4	0.1671360919870767
>500	6.098855666390614E-5	0.03599277897642542
>1k	3.049427369713001E-5	0.038903381555725286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.336993273380348E-5	0.0	0.0	0.0
3	0.0	8.336993273380348E-5	0.0	0.0	0.0
4	0.0	1.3339189237408555E-4	0.0	1.6673986546760694E-5	0.0
5	1.6673986546760694E-5	1.3339189237408555E-4	0.0	1.6673986546760694E-5	0.0
6	1.6673986546760694E-5	1.3339189237408555E-4	0.0	1.6673986546760694E-5	0.0
7	1.6673986546760694E-5	1.3339189237408555E-4	0.0	1.6673986546760694E-5	0.0
8	1.6673986546760694E-5	1.6673986546760695E-4	0.0	3.334797309352139E-5	0.0
9	1.6673986546760694E-5	3.168057443884532E-4	0.0	5.002195964028208E-5	0.0
10-11	1.6673986546760694E-5	3.168057443884532E-4	0.0	6.669594618704278E-5	0.0
12-13	1.6673986546760694E-5	3.168057443884532E-4	0.0	8.336993273380346E-5	0.0
14-15	1.6673986546760694E-5	4.5019763676253875E-4	0.0	1.0004391928056416E-4	0.0
16-17	1.6673986546760694E-5	4.5019763676253875E-4	0.0	1.1671790582732487E-4	0.0
18-19	1.6673986546760694E-5	4.6687162330929946E-4	0.0	1.3339189237408555E-4	0.0
20-21	1.6673986546760694E-5	5.002195964028209E-4	0.0	1.3339189237408555E-4	0.0
22-23	1.6673986546760694E-5	5.585785493164833E-4	0.0	1.4172888564746588E-4	0.0
24-25	1.6673986546760694E-5	5.919265224100046E-4	0.0	2.0008783856112836E-4	0.0
26-27	1.6673986546760694E-5	6.00263515683385E-4	0.0	2.9179476456831215E-4	0.0
28-29	1.6673986546760694E-5	6.00263515683385E-4	0.0	5.919265224100047E-4	0.0
30-31	1.6673986546760694E-5	6.836334484171885E-4	0.0	0.0018674864932371978	0.0
32-33	1.6673986546760694E-5	6.919704416905688E-4	0.0	0.004643705253272854	0.0
34-35	2.501097982014104E-5	8.17025340791274E-4	0.0	0.008228612360826403	0.0
36-37	4.168496636690173E-5	9.337432466185989E-4	0.0	0.013756038901077572	0.0
38-39	6.669594618704278E-5	9.670912197121202E-4	0.0	0.02613647391204739	0.0
40-41	6.669594618704278E-5	0.001033787165899163	0.0	0.03887539963377255	0.0
42-43	6.669594618704278E-5	0.001075472132266065	0.0	0.04812112517395137	0.0
44-45	8.336993273380348E-5	0.0012088640246401502	0.0	0.06000134058851836	0.0
46-47	8.336993273380348E-5	0.0013172449371940949	0.0	0.07203162188200621	0.0
48-49	8.336993273380348E-5	0.0013255819304674754	0.0	0.08381179337729264	0.0
50-51	8.336993273380348E-5	0.001508995782481843	0.0	0.09576704173132006	0.0
52-53	8.336993273380348E-5	0.001534006762301984	0.0	0.11998600719048996	0.0
54-55	8.336993273380348E-5	0.0015423437555753643	0.0	0.14917382064059456	0.0
56-57	8.336993273380348E-5	0.0015673547353955053	0.0	0.1789785715929293	0.0
58-59	8.336993273380348E-5	0.0015756917286688858	0.0	0.2024388706642216	0.0
60-61	8.336993273380348E-5	0.0016423876748559284	0.0	0.21956305484774483	0.0
62-63	8.336993273380348E-5	0.0016507246681293087	0.0	0.23199351181835492	0.0
64-65	8.336993273380348E-5	0.00191750845287748	0.0	0.2374875903855126	0.0
66-67	1.0004391928056416E-4	0.0019508564259710013	0.0	0.24355692148853347	0.0
68-69	1.0004391928056416E-4	0.001992541392337903	0.0	0.2493177838404393	0.0
70-71	1.0004391928056416E-4	0.002025889365431424	0.0	0.25454507862284875	0.0
72-73	1.0004391928056416E-4	0.0020342263587048047	0.0	0.25878860819899935	0.0
74-75	1.0004391928056416E-4	0.0020592373385249457	0.0	0.2617148928379559	0.0
76-77	1.0004391928056416E-4	0.002200966224172412	0.0	0.26480791734237996	0.0
78-79	1.0004391928056416E-4	0.002284336156906215	0.0	0.2667254257952575	0.0
80-81	1.0004391928056416E-4	0.002301010143452976	0.0	0.2673423632974876	0.0
82-83	1.0004391928056416E-4	0.0023260211232731167	0.0	0.26782590890734365	0.0
84-85	1.0838091255394451E-4	0.0023426951098198777	0.0	0.26798431177953785	0.0
86-87	1.584028721942266E-4	0.002467750008920583	0.0	0.2680343337391782	0.0
88	1.8341385201436764E-4	0.0026344898743881897	0.0	0.2680343337391782	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8940	0.0	49.34292	1
GTATCAA	15750	0.0	34.479725	1
ATCAACG	18090	0.0	28.954296	3
TCAACGC	18220	0.0	28.747465	4
CAACGCA	18590	0.0	28.26321	5
TATCAAC	19080	0.0	27.811094	2
AACGCAG	19115	0.0	27.531296	6
ACGCAGA	21920	0.0	23.969173	7
CGCAGAG	22250	0.0	23.613678	8
GTGGTAT	4310	0.0	22.059761	1
TGGTATC	4330	0.0	20.869867	2
GCAGAGT	25630	0.0	20.449463	9
GAGTACT	14340	0.0	20.126108	12-13
TACATGG	18190	0.0	20.036505	2
GTACATG	18730	0.0	19.961887	1
ACATGGG	18570	0.0	19.188301	3
CAGAGTA	25460	0.0	18.862097	10-11
AGAGTAC	22990	0.0	18.187891	10-11
GTACTTT	16055	0.0	17.790276	14-15
CATGGGG	13865	0.0	16.896196	4
>>END_MODULE