FastQCFastQC Report
Fri 27 May 2016
SRR2138662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6480905
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95950.050.7227061
GTATCAA176900.038.905141
ATCAACG221250.030.703793
TCAACGC221550.030.6430634
TATCAAC225700.030.2864172
CAACGCA226400.029.9998045
AACGCAG232050.029.2510836
GTGGTAT46750.027.137731
TGGTATC43150.026.8451482
ACGCAGA265800.025.489047
CGCAGAG268450.025.1900168
GCAGAGT309900.021.779719
TACATGG237050.020.7814672
GTACATG245700.020.7233521
GAGTACT167450.020.52122912-13
ACATGGG242650.019.8297253
AGAGTAC282350.018.93906610-11
CAGAGTA304300.018.85553610-11
GTACTTT189000.018.18144414-15
CATGGGG167200.017.4596734
Produced by FastQC (version 0.11.5)