##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138662_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6480905
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83966421356276	33.0	33.0	33.0	33.0	33.0
2	32.8356099958262	33.0	33.0	33.0	33.0	33.0
3	32.83415464352586	33.0	33.0	33.0	33.0	33.0
4	36.79061118778936	37.0	37.0	37.0	37.0	37.0
5	36.80115477699488	37.0	37.0	37.0	37.0	37.0
6	36.812345035145555	37.0	37.0	37.0	37.0	37.0
7	36.80165300988057	37.0	37.0	37.0	37.0	37.0
8	36.79721489514196	37.0	37.0	37.0	37.0	37.0
9	36.79184018281398	37.0	37.0	37.0	37.0	37.0
10-11	36.76995435051123	37.0	37.0	37.0	37.0	37.0
12-13	36.75665489619119	37.0	37.0	37.0	37.0	37.0
14-15	39.117651161373296	40.0	40.0	40.0	37.0	40.0
16-17	39.0780742812925	40.0	40.0	40.0	37.0	40.0
18-19	39.10317910847328	40.0	40.0	40.0	37.0	40.0
20-21	39.14183951658513	40.0	40.0	40.0	37.0	40.0
22-23	39.16331052935708	40.0	40.0	40.0	37.0	40.0
24-25	39.08563361492283	40.0	40.0	40.0	37.0	40.0
26-27	39.00841203246195	40.0	40.0	40.0	37.0	40.0
28-29	38.975674940740305	40.0	40.0	40.0	37.0	40.0
30-31	38.92767513743972	40.0	40.0	40.0	37.0	40.0
32-33	38.78724428078157	40.0	40.0	40.0	37.0	40.0
34-35	38.85942934965561	40.0	40.0	40.0	37.0	40.0
36-37	38.587367011784025	40.0	37.0	40.0	37.0	40.0
38-39	38.74263525120989	40.0	37.0	40.0	37.0	40.0
40-41	38.74652293396464	40.0	37.0	40.0	37.0	40.0
42-43	38.69291564968602	40.0	37.0	40.0	37.0	40.0
44-45	38.60853575285134	40.0	37.0	40.0	37.0	40.0
46-47	38.53234996784331	40.0	37.0	40.0	37.0	40.0
48-49	38.48128088642133	40.0	37.0	40.0	37.0	40.0
50-51	38.419166280341145	40.0	37.0	40.0	37.0	40.0
52-53	38.35987144371158	40.0	37.0	40.0	37.0	40.0
54-55	38.27807084239846	40.0	37.0	40.0	37.0	40.0
56-57	38.15885289585857	40.0	37.0	40.0	37.0	40.0
58-59	38.026710177632566	40.0	37.0	40.0	37.0	40.0
60-61	37.86955300438346	40.0	37.0	40.0	35.0	40.0
62-63	37.67810378178507	38.5	37.0	40.0	33.0	40.0
64-65	37.45446608776675	37.0	37.0	40.0	33.0	40.0
66-67	37.21414701493458	37.0	37.0	40.0	33.0	40.0
68-69	36.95607323238474	37.0	37.0	40.0	33.0	40.0
70-71	36.702207728975175	37.0	37.0	40.0	33.0	40.0
72-73	36.45124222657228	37.0	37.0	37.0	33.0	40.0
74-75	36.20620951136631	37.0	37.0	37.0	33.0	40.0
76-77	34.5314740454364	35.0	33.0	37.0	30.0	37.0
78-79	35.813451670975944	37.0	37.0	37.0	33.0	37.0
80-81	35.92787624636246	37.0	37.0	37.0	33.0	37.0
82-83	35.84735866776279	37.0	37.0	37.0	33.0	37.0
84-85	35.733954934204675	37.0	37.0	37.0	33.0	37.0
86-87	35.649466499057915	37.0	37.0	37.0	33.0	37.0
88-89	35.57254124620633	37.0	37.0	37.0	33.0	37.0
90-91	35.50787473670277	37.0	35.0	37.0	33.0	37.0
92-93	35.44551384650801	37.0	33.0	37.0	33.0	37.0
94-95	35.39810774636588	37.0	33.0	37.0	33.0	37.0
96-97	35.355161071314	37.0	33.0	37.0	33.0	37.0
98-99	35.31094992688362	37.0	33.0	37.0	33.0	37.0
100	35.21501654501656	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	22.0
21	82.0
22	358.0
23	1032.0
24	2547.0
25	5322.0
26	9769.0
27	15977.0
28	24462.0
29	35462.0
30	48257.0
31	65623.0
32	88348.0
33	121075.0
34	174448.0
35	273063.0
36	557174.0
37	1817732.0
38	3088600.0
39	151548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.13403837889924	20.01735868678834	14.449062283739694	23.39954065057272
2	14.795942850574109	20.751793152345236	41.751468352028	22.70079564505266
3	18.31772349579507	26.02513960748044	30.38419811972166	25.272938777002828
4	12.56396137347269	17.961702500674413	38.676606851066374	30.797729274786516
5	13.46669832912633	37.66666188335161	35.20644864933841	13.660191138183645
6	29.913877768614107	37.57353332597839	18.754896114045803	13.7576927913617
7	26.651185289708767	32.86707643454116	22.692633204776186	17.789105070973886
8	24.67751031684618	36.339554429512546	20.935548353200673	18.0473869004406
9	25.759025938507047	17.09466501977733	21.128978128826144	36.01733091288948
10-11	24.306157859126156	26.84319551050355	28.47883898930782	20.371807641062475
12-13	25.266347832594366	24.401314322613892	28.67619568563341	21.65614215915833
14-15	22.427870798908486	25.725589250266744	26.582252015729285	25.264287935095485
16-17	21.726826731760458	28.928938165271674	27.21623754707097	22.1279975558969
18-19	21.78677977844144	27.922489220255503	29.103790288547664	21.186940712755394
20-21	22.743890005478494	27.00851307528528	29.006307944757708	21.24128897447852
22-23	22.440796372513187	26.934524739524562	28.880250545895287	21.744428342066964
24-25	22.171126070114823	27.11219180754788	28.932583229095023	21.78409889324227
26-27	22.06556108655402	27.34461434589093	28.861679016094815	21.72814555146023
28-29	21.97925990332234	27.37941362471187	28.919804603927012	21.721521868038778
30-31	22.297984107029333	27.197796946540663	28.83118913269667	21.673029813733333
32-33	22.09029463256946	27.38002666031144	28.724384571503798	21.805294135615302
34-35	22.16790871122159	27.32375460446797	28.7843504951297	21.72398618918074
36-37	22.191265985447316	27.364623773609235	28.64988394728785	21.79422629365559
38-39	21.989711100890535	27.375492214156203	28.79075757758953	21.84403910736373
40-41	22.26237575919067	27.30541134762029	28.60690590788809	21.825306985300948
42-43	22.19717214040755	27.321170443319332	28.476748721513346	22.004908694759777
44-45	22.24713830973705	27.523626994447838	28.250676287113297	21.978558408701822
46-47	22.388840747518863	27.41672200851491	27.892940369983098	22.301496873983123
48-49	22.320772409238256	27.59245710637122	27.851871738735706	22.23489874565482
50-51	22.20907163559462	27.726083286228604	27.778569052779385	22.286276025397395
52-53	22.367167804104266	27.70336617537499	27.63191227795741	22.297553742563338
54-55	22.436978046627303	27.585872224371542	27.60355518004906	22.37359454895209
56-57	22.461243106722996	27.7352122216615	27.51595200348908	22.287592668126425
58-59	22.32030945992996	27.87748069715526	27.625570893153377	22.1766389497614
60-61	22.46333675086279	27.82933728053968	27.512736525009668	22.194589443587862
62-63	22.397093053404884	27.908742459649073	27.41055617425176	22.283608312694284
64-65	22.36589179873272	27.890039264178583	27.429942711655325	22.314126225433377
66-67	22.477494581086333	27.901541724086353	27.26844267192051	22.352521022906803
68-69	22.440388518141503	27.83180917172356	27.34144916007774	22.386353150057193
70-71	22.43326603696146	27.55935460515287	27.43308358527322	22.574295772612448
72-73	22.506105106617376	27.596048767152926	27.351917472768168	22.545928653461534
74-75	22.358814131903017	27.812574477748676	27.299845252044676	22.528766138303638
76-77	22.49232888384272	27.752526804170575	27.19478885594978	22.56035545603692
78-79	22.475053160777907	27.752141686968685	27.159501110156643	22.613304042096765
80-81	22.493817596249546	27.745514264899935	27.175652557020562	22.58501558182996
82-83	22.504795676287678	27.685315038009676	27.307020893097505	22.502868392605137
84-85	22.627798132649673	27.679705478797562	27.213749801571495	22.47874658698127
86-87	22.442640928653844	27.79041265942351	27.255034234626635	22.51191217729601
88-89	22.521397159109018	27.724968872629912	27.241789333285286	22.51184463497578
90-91	22.53777438575146	27.778151194508926	27.27581164129943	22.40826277844019
92-93	22.64795739377984	27.73993661725347	27.24066589308627	22.371440095880427
94-95	22.452498672845834	27.81423233446032	27.2341901635907	22.49907882910314
96-97	22.49305207478844	27.891915533663887	27.405372336349508	22.209660055198164
98-99	23.680110788067392	29.778044221165896	27.519388761742757	19.022456229023955
100	22.543042467681023	30.842709951579156	21.407226962080237	25.207020618659588
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2448.0
1	2042.0
2	2140.5
3	2776.0
4	3608.0
5	5559.5
6	7263.5
7	8601.5
8	9669.0
9	9898.0
10	9340.0
11	8809.5
12	9879.0
13	12927.0
14	19791.5
15	29044.0
16	36640.0
17	41203.5
18	41947.0
19	39565.0
20	35578.5
21	31365.5
22	29068.0
23	30249.5
24	34783.0
25	42842.0
26	54639.5
27	68115.5
28	80710.5
29	94722.0
30	107411.5
31	118912.5
32	132824.0
33	146330.0
34	161147.5
35	175340.5
36	190101.5
37	203368.0
38	209988.0
39	216029.0
40	225852.5
41	234317.0
42	238929.5
43	244508.0
44	251171.5
45	259203.0
46	265873.5
47	268951.5
48	269151.5
49	266738.0
50	263262.0
51	257117.0
52	248680.5
53	238185.0
54	225817.5
55	211850.0
56	196929.0
57	178900.5
58	159414.0
59	139974.5
60	117782.5
61	95971.0
62	75969.0
63	58429.0
64	43491.0
65	32027.0
66	24195.5
67	18450.5
68	13853.5
69	9978.0
70	7131.0
71	5065.0
72	3550.5
73	2430.5
74	1631.0
75	1077.0
76	718.0
77	479.0
78	320.0
79	212.5
80	133.5
81	82.5
82	63.0
83	45.5
84	31.0
85	22.5
86	18.5
87	16.5
88	12.0
89	7.0
90	3.5
91	1.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	8.640768534641382E-4
4	0.018716521843785708
5	6.789175277218229E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.007724666047098546
26-27	0.05090151806791609
28-29	0.08461965219592864
30-31	0.07502455000929108
32-33	0.035402229937483445
34-35	0.004275179466204202
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.10373495915359E-4
56-57	7.443677162984556E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.612903412278886E-5
72-73	0.013944505791757912
74-75	0.029211606094242552
76-77	0.0035787500699482966
78-79	0.0
80-81	0.0
82-83	0.010948840726772926
84-85	0.05074673446846258
86-87	0.04147307832431144
88-89	0.01017512498676145
90-91	1.6027398838314096E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	62.0
20-21	285.0
22-23	719.0
24-25	2077.0
26-27	2886.0
28-29	5794.0
30-31	13597.0
32-33	17545.0
34-35	24456.0
36-37	35180.0
38-39	40393.0
40-41	43447.0
42-43	51807.0
44-45	62024.0
46-47	61666.0
48-49	54426.0
50-51	47511.0
52-53	44794.0
54-55	48218.0
56-57	51724.0
58-59	51830.0
60-61	51524.0
62-63	51368.0
64-65	53732.0
66-67	57937.0
68-69	56537.0
70-71	54013.0
72-73	51387.0
74-75	52309.0
76-77	52156.0
78-79	55052.0
80-81	55770.0
82-83	53579.0
84-85	53281.0
86-87	55528.0
88-89	59445.0
90-91	61530.0
92-93	63211.0
94-95	74791.0
96-97	206429.0
98-99	392367.0
100-101	4208518.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.69976837908466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.88184053333083	77.02852915192277
2	7.210305465730245	12.358430167110601
3	1.6745103235551217	4.305154406311802
4	0.5987457549298352	2.0524949006178823
5	0.2638038735663698	1.1303965431071612
6	0.13309559864606937	0.6843757185746255
7	0.06844062259868743	0.4105741853099501
8	0.04536305914648476	0.3110082929456369
9	0.03133673931428735	0.2416996170891164
>10	0.09025323345178869	1.1693420236989096
>50	0.0014547146328598916	0.08405010558410028
>100	6.576181738009623E-4	0.09477313821032181
>500	1.6496825496228727E-4	0.08935050368468096
>1k	2.749466865798886E-5	0.039821245832473574
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.542994381185961E-5	0.0	0.0	0.0
3	0.0	1.542994381185961E-5	0.0	0.0	0.0
4	0.0	7.714971905929804E-5	0.0	0.0	0.0
5	0.0	7.714971905929804E-5	0.0	0.0	0.0
6	0.0	7.714971905929804E-5	0.0	0.0	0.0
7	0.0	7.714971905929804E-5	0.0	0.0	0.0
8	0.0	9.257966287115766E-5	0.0	0.0	0.0
9	0.0	2.7773898861347296E-4	0.0	0.0	0.0
10-11	1.542994381185961E-5	2.7773898861347296E-4	0.0	7.714971905929805E-6	0.0
12-13	3.085988762371922E-5	2.7773898861347296E-4	0.0	1.542994381185961E-5	0.0
14-15	3.085988762371922E-5	3.240288200490518E-4	0.0	3.857485952964902E-5	0.0
16-17	3.085988762371922E-5	3.240288200490518E-4	0.0	4.628983143557883E-5	0.0
18-19	3.085988762371922E-5	3.780336233905604E-4	0.0	4.628983143557883E-5	0.0
20-21	3.085988762371922E-5	4.397533986379989E-4	0.0	4.628983143557883E-5	0.0
22-23	3.085988762371922E-5	5.400480334150863E-4	0.0	5.400480334150864E-5	0.0
24-25	3.085988762371922E-5	5.786228929447353E-4	0.0	8.486469096522786E-5	0.0
26-27	3.085988762371922E-5	6.017678086625248E-4	0.0	1.465844662126663E-4	0.0
28-29	3.085988762371922E-5	6.017678086625248E-4	0.0	5.014731738854373E-4	0.0
30-31	4.628983143557883E-5	6.171977524743843E-4	0.0	0.0016278590721511888	0.0
32-33	4.628983143557883E-5	6.171977524743843E-4	0.0	0.004389819014474059	0.0
34-35	6.171977524743844E-5	7.252073591574016E-4	0.0	0.008147010332661873	0.0
36-37	7.714971905929804E-5	8.409319377463487E-4	0.0	0.014334417801217577	0.0
38-39	7.714971905929804E-5	9.103666848997169E-4	0.0	0.029316893242533254	0.0
40-41	7.714971905929804E-5	0.0010029463477708746	0.0	0.04449995795340311	0.0
42-43	7.714971905929804E-5	0.0010646661230183131	0.0	0.0539970883696027	0.0
44-45	7.714971905929804E-5	0.0012498254487606285	0.0	0.06626389370003109	0.0
46-47	7.714971905929804E-5	0.0013809799711614352	0.0	0.0788470128786026	0.0
48-49	7.714971905929804E-5	0.0013886949430673649	0.0	0.09074349955754636	0.0
50-51	7.714971905929804E-5	0.0016664339316808377	0.0	0.10243939696693595	0.0
52-53	9.257966287115766E-5	0.0016818638754926975	0.0	0.1334921588883034	0.0
54-55	9.257966287115766E-5	0.0016818638754926975	0.0	0.17173527462599744	0.0
56-57	9.257966287115766E-5	0.001697293819304557	0.0	0.21004011013893892	0.0
58-59	9.257966287115766E-5	0.0017127237631164166	0.0	0.23465858549076093	0.0
60-61	9.257966287115766E-5	0.0017590135945519953	0.0	0.2509063163246491	0.0
62-63	9.257966287115766E-5	0.0017744435383638551	0.0	0.2616455572177034	0.0
64-65	9.257966287115766E-5	0.0019441729202943107	0.0	0.2675012208943041	0.0
66-67	9.257966287115766E-5	0.0019596028641061703	0.0	0.2729248461441728	0.0
68-69	9.257966287115766E-5	0.00197503280791803	0.0	0.27819417195592283	0.0
70-71	9.257966287115766E-5	0.0019827477798239597	0.0	0.28322433363858907	0.0
72-73	9.257966287115766E-5	0.001998177723635819	0.0	0.2872515489734844	0.0
74-75	9.257966287115766E-5	0.0020290376112595387	0.0	0.2902526730448911	0.0
76-77	9.257966287115766E-5	0.0020753274426951176	0.0	0.29300691801530804	0.0
78-79	1.0800960668301726E-4	0.0020984723584129067	0.0	0.2949048011041668	0.0
80-81	1.0800960668301726E-4	0.0020984723584129067	0.0	0.2955528587442649	0.0
82-83	1.1572457858894706E-4	0.0021139023022247664	0.0	0.29605433191815034	0.0
84-85	1.2343955049487688E-4	0.0021293322460366262	0.0	0.2963552158224816	0.0
86-87	1.2343955049487688E-4	0.0021293322460366262	0.0	0.2964555104572587	0.0
88	1.2343955049487688E-4	0.0022373418527196435	0.0	0.2964709404010705	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9595	0.0	50.722706	1
GTATCAA	17690	0.0	38.90514	1
ATCAACG	22125	0.0	30.70379	3
TCAACGC	22155	0.0	30.643063	4
TATCAAC	22570	0.0	30.286417	2
CAACGCA	22640	0.0	29.999804	5
AACGCAG	23205	0.0	29.251083	6
GTGGTAT	4675	0.0	27.13773	1
TGGTATC	4315	0.0	26.845148	2
ACGCAGA	26580	0.0	25.48904	7
CGCAGAG	26845	0.0	25.190016	8
GCAGAGT	30990	0.0	21.77971	9
TACATGG	23705	0.0	20.781467	2
GTACATG	24570	0.0	20.723352	1
GAGTACT	16745	0.0	20.521229	12-13
ACATGGG	24265	0.0	19.829725	3
AGAGTAC	28235	0.0	18.939066	10-11
CAGAGTA	30430	0.0	18.855536	10-11
GTACTTT	18900	0.0	18.181444	14-15
CATGGGG	16720	0.0	17.459673	4
>>END_MODULE