FastQCFastQC Report
Fri 27 May 2016
SRR2138661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5942278
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89650.049.785961
GTATCAA161700.037.9142651
ATCAACG195900.030.5429483
TCAACGC200400.029.8568524
CAACGCA202050.029.60835
TATCAAC204150.029.514922
AACGCAG209800.028.67516
ACGCAGA231150.025.9536957
CGCAGAG235450.025.4618248
GCAGAGT271700.022.033749
CTTATAC88150.021.3996681
GTGGTAT47900.020.9214971
GAGTACT159900.020.60222412-13
TATACAC101600.020.514593
TGGTATC46200.020.3242362
TACATGG191700.019.856272
CAGAGTA263950.019.65818810-11
ACATGGG196500.018.728383
GTACTTT174450.018.70313614-15
GTACATG212150.018.6170621
Produced by FastQC (version 0.11.5)