FastQCFastQC Report
Fri 27 May 2016
SRR2138660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6434184
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75000.050.6953961
GTATCAA145250.038.2285271
ATCAACG165300.032.7849963
TCAACGC168750.032.2419174
CAACGCA169400.032.0601965
AACGCAG174100.031.2186726
TATCAAC177500.030.7739222
ACGCAGA200350.027.0854557
CGCAGAG204400.026.527748
GCAGAGT239000.022.5613849
GAGTACT138650.020.48420312-13
TACATGG176100.020.4673772
GTACATG180800.020.2207831
GTGGTAT38700.019.8938831
CAGAGTA234900.019.32152210-11
TGGTATC37700.019.1666162
ACATGGG181950.018.9346793
AGAGTAC215050.018.81552310-11
GTACTTT155950.017.9640914-15
CATGGGG130950.017.6377374
Produced by FastQC (version 0.11.5)