##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138660_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6434184
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84100439154367	33.0	33.0	33.0	33.0	33.0
2	32.841534062438996	33.0	33.0	33.0	33.0	33.0
3	32.8431465435244	33.0	33.0	33.0	33.0	33.0
4	36.79632335662144	37.0	37.0	37.0	37.0	37.0
5	36.80575034845133	37.0	37.0	37.0	37.0	37.0
6	36.81482531429005	37.0	37.0	37.0	37.0	37.0
7	36.80569128890315	37.0	37.0	37.0	37.0	37.0
8	36.800572224854	37.0	37.0	37.0	37.0	37.0
9	36.79639065342241	37.0	37.0	37.0	37.0	37.0
10-11	36.77609965770329	37.0	37.0	37.0	37.0	37.0
12-13	36.763935488944675	37.0	37.0	37.0	37.0	37.0
14-15	39.14187774238349	40.0	40.0	40.0	37.0	40.0
16-17	39.099622656113034	40.0	40.0	40.0	37.0	40.0
18-19	39.11648229208242	40.0	40.0	40.0	37.0	40.0
20-21	39.1557678124551	40.0	40.0	40.0	37.0	40.0
22-23	39.17494431137807	40.0	40.0	40.0	37.0	40.0
24-25	39.095557505410085	40.0	40.0	40.0	37.0	40.0
26-27	39.01951715181599	40.0	40.0	40.0	37.0	40.0
28-29	38.99263647944803	40.0	40.0	40.0	37.0	40.0
30-31	38.95556577852754	40.0	40.0	40.0	37.0	40.0
32-33	38.81159211315241	40.0	40.0	40.0	37.0	40.0
34-35	38.884763577052645	40.0	40.0	40.0	37.0	40.0
36-37	38.59175698867635	40.0	37.0	40.0	37.0	40.0
38-39	38.7562331868515	40.0	37.0	40.0	37.0	40.0
40-41	38.770891863002205	40.0	37.0	40.0	37.0	40.0
42-43	38.72550845689592	40.0	37.0	40.0	37.0	40.0
44-45	38.64224541191969	40.0	37.0	40.0	37.0	40.0
46-47	38.56905692057131	40.0	37.0	40.0	37.0	40.0
48-49	38.514814089429336	40.0	37.0	40.0	37.0	40.0
50-51	38.440138694786796	40.0	37.0	40.0	37.0	40.0
52-53	38.392204156009555	40.0	37.0	40.0	37.0	40.0
54-55	38.314650211332975	40.0	37.0	40.0	37.0	40.0
56-57	38.181336409039375	40.0	37.0	40.0	37.0	40.0
58-59	38.06093434917587	40.0	37.0	40.0	37.0	40.0
60-61	37.90145217511988	40.0	37.0	40.0	35.0	40.0
62-63	37.726016254501644	40.0	37.0	40.0	33.0	40.0
64-65	37.52218833325371	37.0	37.0	40.0	33.0	40.0
66-67	37.29147790144894	37.0	37.0	40.0	33.0	40.0
68-69	37.04021202601112	37.0	37.0	40.0	33.0	40.0
70-71	36.79028319319695	37.0	37.0	40.0	33.0	40.0
72-73	36.54079248908539	37.0	37.0	38.5	33.0	40.0
74-75	36.280973861619316	37.0	37.0	37.0	33.0	40.0
76-77	34.60360238027035	35.0	33.0	37.0	30.0	37.0
78-79	35.852653139774205	37.0	37.0	37.0	33.0	37.0
80-81	35.94665998368045	37.0	37.0	37.0	33.0	37.0
82-83	35.86018138533777	37.0	37.0	37.0	33.0	37.0
84-85	35.75013165224887	37.0	37.0	37.0	33.0	37.0
86-87	35.665235514523985	37.0	37.0	37.0	33.0	37.0
88-89	35.59185387990415	37.0	37.0	37.0	33.0	37.0
90-91	35.53380635878697	37.0	37.0	37.0	33.0	37.0
92-93	35.477071962479314	37.0	33.0	37.0	33.0	37.0
94-95	35.41252413311304	37.0	33.0	37.0	33.0	37.0
96-97	35.35998489322411	37.0	33.0	37.0	33.0	37.0
98-99	35.321235910432904	37.0	33.0	37.0	33.0	37.0
100	35.22097535445028	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	18.0
21	76.0
22	351.0
23	1086.0
24	2618.0
25	5432.0
26	9903.0
27	16466.0
28	24538.0
29	34464.0
30	47964.0
31	63563.0
32	85863.0
33	118080.0
34	170026.0
35	266927.0
36	544034.0
37	1790260.0
38	3112121.0
39	140389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.219881184622636	20.343076915425485	14.472200359828069	23.964841540123814
2	14.350397812683008	20.542589394397176	42.29069296122088	22.81631983169894
3	18.140509990567473	25.876969325559568	30.541430769309674	25.441089914563285
4	12.2785428786752	18.24638169594556	38.7666706876954	30.708404737683836
5	13.329088263823042	37.57307707909584	35.69484363933529	13.40299101774583
6	29.638599082649797	37.787386869881246	18.86820457730149	13.705809470167468
7	26.377377457654305	32.59841496606251	22.689233009189667	18.33497456709351
8	24.20683026783194	36.762221907237965	21.19465343235444	17.836294392575656
9	24.99863230519985	17.31276258185964	21.308684986316837	36.379920126623674
10-11	23.979792931007257	26.837505734992966	28.809193209270983	20.373508124728794
12-13	24.941033703729953	24.13687578720161	28.905747799565574	22.01634270950287
14-15	22.09268494652935	25.821906864957544	26.341055835518535	25.744352352994564
16-17	21.7587731404635	29.165065842071037	26.979769307187983	22.09639171027748
18-19	21.835418446224107	28.075914521561707	28.672190599460627	21.416476432753555
20-21	22.42489096119287	27.123679634756424	28.742097236388364	21.709332167662343
22-23	22.15199582947087	27.405818037351974	28.481261839969314	21.960924293207846
24-25	22.175950390211618	27.45407965046292	28.53567141599424	21.834298543331222
26-27	22.064580386640348	27.604616011823097	28.594712634182706	21.73609096735385
28-29	21.946959672921512	27.428292762525857	28.75296188329845	21.87178568125418
30-31	21.95983889173072	27.15829563884865	28.89820353868372	21.98366193073691
32-33	21.745591493817244	27.5540017761023	28.687822271748338	22.01258445833212
34-35	22.04071121619547	27.472238119987413	28.728062453411983	21.758988210405136
36-37	21.972288422581716	27.505042771739106	28.692014017235966	21.830654788443205
38-39	21.857963382195475	27.34239612027709	28.73894013052875	22.060700366998685
40-41	22.10689835624501	27.1978528941623	28.83976808731991	21.85548066227278
42-43	22.0666137643858	27.242556425551484	28.721635542668828	21.96919426739389
44-45	21.938975591411513	27.525485316081657	28.483848925127376	22.051690167379455
46-47	22.265737157965606	27.23960071596721	28.056950859167962	22.437711266899218
48-49	22.123921293509383	27.458455022257443	28.07736895398817	22.340254730245007
50-51	21.899943877824203	27.667819517957444	28.026647787404702	22.405588816813648
52-53	21.917688331514185	27.667338798235384	28.113771735500066	22.30120113475036
54-55	22.090480589426086	27.432897339299306	28.076223011819895	22.400399059454713
56-57	22.24222234363668	27.317903884156635	27.896244193415892	22.543629578790792
58-59	21.714302098396132	27.418643276824938	28.345713636303397	22.52134098847553
60-61	22.119985781887397	27.89167081893691	28.06008034695421	21.92826305222149
62-63	21.51122867670573	28.26918855727687	28.022000446131567	22.197582319885836
64-65	21.376211732533665	28.42305517169525	27.809583754412486	22.3911493413586
66-67	21.532707633551222	28.718207419408355	27.442209190859256	22.306875756181164
68-69	21.64105407429367	28.512468686028527	27.33029162211641	22.516185617561398
70-71	21.665071225243114	27.9805615522011	27.3088258506406	23.045541371915185
72-73	21.919449726129713	27.437228765579825	27.358372253974046	23.284949254316416
74-75	22.000846437120853	27.555741482805583	27.52544442243415	22.91796765763942
76-77	22.289455269993002	27.45919474218631	26.87465161236761	23.376698375453074
78-79	22.488814217216273	27.46301610614554	26.470681159480364	23.57748851715783
80-81	22.320642767907344	27.81286092215754	26.69625330874872	23.1702430011864
82-83	22.296421737763307	27.617326025558413	26.770831130143534	23.315421106534743
84-85	22.43764242864856	28.00106748773315	26.667011732392982	22.89427835122531
86-87	22.789061813572076	27.896833755573436	26.741351943359586	22.572752487494903
88-89	22.59031117601712	27.99041193367673	27.039252261086784	22.38002462921937
90-91	22.532818696333976	27.665238513701368	26.910929056246562	22.891013733718097
92-93	22.565330469401452	27.21917016621203	27.173548080598092	23.04195128378842
94-95	21.836621538083655	27.67977322175993	27.75749055718797	22.726114682968447
96-97	21.952907517622986	27.607882127124846	27.912568104788164	22.526642250464004
98-99	23.156361928640546	29.38100349268828	28.26780578271662	19.194828795954557
100	22.141466321807723	30.437306587641043	21.931694043455504	25.489533047095726
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1208.0
1	1077.5
2	1346.0
3	1877.5
4	2635.0
5	4329.5
6	5687.0
7	6570.5
8	7141.0
9	7041.5
10	6618.5
11	6309.0
12	7146.0
13	9707.5
14	15261.5
15	22608.0
16	28791.0
17	32654.0
18	33393.0
19	31686.0
20	28895.5
21	25762.5
22	24182.0
23	25875.0
24	30540.5
25	37568.0
26	47747.0
27	60283.5
28	73423.5
29	88056.5
30	101427.5
31	114144.5
32	128363.5
33	141837.0
34	156383.0
35	171101.5
36	185769.5
37	199983.5
38	210949.5
39	219898.5
40	229554.0
41	238350.5
42	245434.0
43	252539.5
44	259459.5
45	266373.5
46	274659.0
47	283237.5
48	294978.0
49	294923.5
50	281004.5
51	269435.5
52	255100.5
53	236153.0
54	217813.0
55	198371.5
56	178751.0
57	160259.0
58	140570.5
59	120648.5
60	98071.0
61	77070.0
62	60318.0
63	46136.0
64	34490.5
65	25443.0
66	18959.5
67	14620.5
68	11067.0
69	7962.0
70	5703.5
71	3987.5
72	2730.0
73	1826.5
74	1223.5
75	817.0
76	528.5
77	318.0
78	207.0
79	141.5
80	91.0
81	55.5
82	41.5
83	31.5
84	23.5
85	18.5
86	15.0
87	12.0
88	7.0
89	2.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001010229113746203
4	0.020764093784075805
5	8.237252773622887E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.771139325182238E-6
22-23	0.0
24-25	0.007220869291617177
26-27	0.05059080921970266
28-29	0.08566507421778406
30-31	0.07598579459664531
32-33	0.03535498953239496
34-35	0.004006232821907458
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.489965439279703E-4
56-57	6.48648378940383E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	7.098075259294908E-5
72-73	0.012978331918017427
74-75	0.02882407534282482
76-77	0.003950137766756316
78-79	0.0
80-81	0.0
82-83	0.010076080991012643
84-85	0.0492852733571805
86-87	0.03998267225772083
88-89	0.010117386884416608
90-91	8.823013006003472E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	70.0
20-21	279.0
22-23	626.0
24-25	1707.0
26-27	2494.0
28-29	5209.0
30-31	12219.0
32-33	16484.0
34-35	23306.0
36-37	33165.0
38-39	23640.0
40-41	40723.0
42-43	47427.0
44-45	56514.0
46-47	56345.0
48-49	51268.0
50-51	27573.0
52-53	8507.0
54-55	10588.0
56-57	13293.0
58-59	36395.0
60-61	58934.0
62-63	60915.0
64-65	64892.0
66-67	72225.0
68-69	60064.0
70-71	54279.0
72-73	50638.0
74-75	50965.0
76-77	51694.0
78-79	54387.0
80-81	54582.0
82-83	52618.0
84-85	52020.0
86-87	54404.0
88-89	58307.0
90-91	60842.0
92-93	62403.0
94-95	73298.0
96-97	217738.0
98-99	430802.0
100-101	4270345.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.26390995013533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.50303360999565	74.57611654426475
2	8.101479154855639	13.653246199353083
3	2.0092677340247365	5.079262662167185
4	0.6771475597964607	2.2823640400657137
5	0.29599762000595053	1.2470958398817895
6	0.1676123701344606	0.8474204198123364
7	0.0830848309246324	0.49007368978788113
8	0.04718159433605167	0.3180564493149499
9	0.028053655056480165	0.21275195971062624
>10	0.08499454355812136	1.0406241292604559
>50	0.001295542018236607	0.07236474241141516
>100	7.946306341171924E-4	0.1440934123847081
>500	2.857737346797723E-5	0.012425492718507822
>1k	2.8577285984348583E-5	0.024104418866572178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.55419863653262E-5	0.0	0.0	0.0
3	0.0	1.55419863653262E-5	0.0	0.0	0.0
4	0.0	1.243358909226096E-4	0.0	0.0	0.0
5	0.0	1.243358909226096E-4	0.0	0.0	1.55419863653262E-5
6	0.0	1.243358909226096E-4	0.0	0.0	1.55419863653262E-5
7	0.0	1.243358909226096E-4	0.0	0.0	1.55419863653262E-5
8	0.0	1.3987787728793582E-4	0.0	0.0	1.55419863653262E-5
9	0.0	2.952977409411978E-4	0.0	0.0	3.10839727306524E-5
10-11	0.0	2.952977409411978E-4	0.0	0.0	3.10839727306524E-5
12-13	0.0	2.952977409411978E-4	0.0	2.33129795479893E-5	3.88549659133155E-5
14-15	0.0	4.1963363186380746E-4	0.0	3.10839727306524E-5	4.66259590959786E-5
16-17	0.0	4.5071760459445984E-4	0.0	3.88549659133155E-5	4.66259590959786E-5
18-19	0.0	4.895725705077753E-4	0.0	5.43969522786417E-5	4.66259590959786E-5
20-21	0.0	5.36198529603754E-4	0.0	6.21679454613048E-5	4.66259590959786E-5
22-23	0.0	5.828244886997326E-4	0.0	9.32519181919572E-5	6.21679454613048E-5
24-25	0.0	6.527634273437005E-4	0.0	1.5541986365326204E-4	9.32519181919572E-5
26-27	0.0	6.91618393257016E-4	0.0	2.2535880229722992E-4	1.087939045572834E-4
28-29	0.0	6.993893864396791E-4	0.0	8.625802432756042E-4	1.087939045572834E-4
30-31	0.0	8.237252773622887E-4	0.0	0.0027198476139320854	1.087939045572834E-4
32-33	0.0	8.237252773622887E-4	0.0	0.006364443416601079	1.087939045572834E-4
34-35	0.0	0.00100245812056354	0.0	0.011190230183034864	1.243358909226096E-4
36-37	0.0	0.0011734199705821283	0.0	0.02042217008403863	1.243358909226096E-4
38-39	0.0	0.0012122749364954436	0.0	0.15536546670098336	1.243358909226096E-4
40-41	0.0	0.0013366108274180534	0.0	0.2941942599092597	1.243358909226096E-4
42-43	0.0	0.0013832367865140318	0.0	0.3144454681432797	1.243358909226096E-4
44-45	0.0	0.0015775116160806095	0.0	0.3427318833281734	1.3210688410527271E-4
46-47	0.0	0.0017407024729165345	0.0	0.37155449704267085	1.4764887047059893E-4
48-49	0.0	0.0017640154524645238	0.0	0.39817791968647465	1.7096185001858822E-4
50-51	0.0	0.0020437712070403955	0.0	0.42409418195065607	1.787328432012513E-4
52-53	0.0	0.0021137101456843634	0.0	0.8706309922128431	1.865038363839144E-4
54-55	0.0	0.002144794118415016	0.0	1.485487514811513	1.865038363839144E-4
56-57	0.0	0.002160336104780342	0.0	2.16809155597664	1.865038363839144E-4
58-59	0.0	0.002168107097963005	0.0	2.8699210342756754	2.2535880229722992E-4
60-61	0.0	0.0021914200775109945	0.0	3.053782732977484	2.486717818452192E-4
62-63	0.0	0.0022691300093376256	0.0	3.0599062756054227	2.6421376821054543E-4
64-65	0.0	0.0025022598048175184	0.0	3.06550139069694	2.6421376821054543E-4
66-67	7.7709931826631E-6	0.0025333437775481708	0.0	3.0709333149316214	2.6421376821054543E-4
68-69	1.55419863653262E-5	0.002618824702557465	0.0	3.076683849886792	2.6421376821054543E-4
70-71	1.55419863653262E-5	0.0026654506616534436	0.0	3.081012293089535	2.6421376821054543E-4
72-73	1.55419863653262E-5	0.0026732216548361067	0.0	3.0846646598853873	3.10839727306524E-4
74-75	1.55419863653262E-5	0.0027276186071147485	0.0	3.087687576233443	3.10839727306524E-4
76-77	1.55419863653262E-5	0.0028441835048546946	0.0	3.090578385697394	3.10839727306524E-4
78-79	1.55419863653262E-5	0.0028908094639506733	0.0	3.0925522179657903	3.10839727306524E-4
80-81	1.55419863653262E-5	0.0029063514503159995	0.0	3.093321546290874	3.10839727306524E-4
82-83	1.55419863653262E-5	0.002937435423046652	0.0	3.0938655158136603	3.10839727306524E-4
84-85	1.55419863653262E-5	0.0029840613821426306	0.0	3.094168584547784	3.10839727306524E-4
86-87	1.55419863653262E-5	0.0030384583344212723	0.0	3.0943162334182546	3.10839727306524E-4
88	1.55419863653262E-5	0.003155023232161219	0.0	3.0943162334182546	3.10839727306524E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7500	0.0	50.695396	1
GTATCAA	14525	0.0	38.228527	1
ATCAACG	16530	0.0	32.784996	3
TCAACGC	16875	0.0	32.241917	4
CAACGCA	16940	0.0	32.060196	5
AACGCAG	17410	0.0	31.218672	6
TATCAAC	17750	0.0	30.773922	2
ACGCAGA	20035	0.0	27.085455	7
CGCAGAG	20440	0.0	26.52774	8
GCAGAGT	23900	0.0	22.561384	9
GAGTACT	13865	0.0	20.484203	12-13
TACATGG	17610	0.0	20.467377	2
GTACATG	18080	0.0	20.220783	1
GTGGTAT	3870	0.0	19.893883	1
CAGAGTA	23490	0.0	19.321522	10-11
TGGTATC	3770	0.0	19.166616	2
ACATGGG	18195	0.0	18.934679	3
AGAGTAC	21505	0.0	18.815523	10-11
GTACTTT	15595	0.0	17.96409	14-15
CATGGGG	13095	0.0	17.637737	4
>>END_MODULE