FastQCFastQC Report
Fri 27 May 2016
SRR2138659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5584660
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC125850.059.3699761
GGTATCA77800.051.152111
TATACAC155800.048.9993173
TTATACA154150.048.4974332
ATACACA195750.039.5509154
GTATCAA144150.037.0028461
ACACATC228950.032.6259236
TACACAT238850.031.9015375
ATCAACG169650.030.8754623
TCAACGC176750.029.6338754
CAACGCA179050.029.2937225
AACGCAG180150.029.184236
TATCAAC181200.029.1608962
ACGCAGA183200.028.6756157
ACATCTC283900.026.3550648
CACATCT297550.025.1040337
CGCAGAG213400.024.6565368
CATCTCC327550.022.7284649
GTGGTAT37050.021.7074551
GCAGAGT246750.021.2396149
Produced by FastQC (version 0.11.5)