FastQCFastQC Report
Fri 27 May 2016
SRR2138658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8148182
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132300.050.1025771
GTATCAA241850.038.3286131
ATCAACG293600.031.0939883
TCAACGC295100.030.9068764
TATCAAC300550.030.5592252
CAACGCA299900.030.4374355
AACGCAG305250.029.9680396
ACGCAGA349650.026.1260347
CGCAGAG355200.025.7058268
GCAGAGT400800.022.781219
GAGTACT242200.021.02108812-13
TACATGG277900.020.663872
GTACATG283250.020.6194861
GTGGTAT66850.020.3919561
TGGTATC65900.019.91022
CAGAGTA393600.019.84916910-11
ACATGGG283500.019.7748473
AGAGTAC370900.018.95127310-11
GTACTTT269600.018.85377114-15
CATGGGG211450.017.8901064
Produced by FastQC (version 0.11.5)