##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138658_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8148182
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78657312760073	33.0	33.0	33.0	33.0	33.0
2	32.78095592366493	33.0	33.0	33.0	33.0	33.0
3	32.77961452014695	33.0	33.0	33.0	33.0	33.0
4	36.722610147883294	37.0	37.0	37.0	37.0	37.0
5	36.738190433154294	37.0	37.0	37.0	37.0	37.0
6	36.747371867736874	37.0	37.0	37.0	37.0	37.0
7	36.73486895604443	37.0	37.0	37.0	37.0	37.0
8	36.72687797106152	37.0	37.0	37.0	37.0	37.0
9	36.72348347152776	37.0	37.0	37.0	37.0	37.0
10-11	36.695065169138346	37.0	37.0	37.0	37.0	37.0
12-13	36.67456127023181	37.0	37.0	37.0	37.0	37.0
14-15	38.92382386647721	40.0	40.0	40.0	37.0	40.0
16-17	38.87766596278777	40.0	38.5	40.0	37.0	40.0
18-19	38.90638568210676	40.0	40.0	40.0	37.0	40.0
20-21	38.940438420088626	40.0	40.0	40.0	37.0	40.0
22-23	38.96553799355547	40.0	40.0	40.0	37.0	40.0
24-25	38.973413906523106	40.0	40.0	40.0	37.0	40.0
26-27	38.879967109879786	40.0	40.0	40.0	37.0	40.0
28-29	38.80639441674504	40.0	38.5	40.0	37.0	40.0
30-31	38.73885297642286	40.0	37.0	40.0	37.0	40.0
32-33	38.419573155489935	40.0	37.0	40.0	37.0	40.0
34-35	38.56252987429128	40.0	37.0	40.0	37.0	40.0
36-37	38.53236005932725	40.0	37.0	40.0	37.0	40.0
38-39	38.56398581984666	40.0	37.0	40.0	37.0	40.0
40-41	38.5304668300474	40.0	37.0	40.0	37.0	40.0
42-43	38.228203091210894	40.0	37.0	40.0	37.0	40.0
44-45	38.13280651208923	40.0	37.0	40.0	35.0	40.0
46-47	38.15595329199071	40.0	37.0	40.0	37.0	40.0
48-49	38.022891314919974	40.0	37.0	40.0	35.0	40.0
50-51	38.12092860486102	40.0	37.0	40.0	37.0	40.0
52-53	38.10025397691227	40.0	37.0	40.0	37.0	40.0
54-55	38.03853221656945	40.0	37.0	40.0	37.0	40.0
56-57	37.92348143003142	40.0	37.0	40.0	33.0	40.0
58-59	37.7915413415965	40.0	37.0	40.0	33.0	40.0
60-61	37.54687954170896	38.5	37.0	40.0	33.0	40.0
62-63	37.355606192631775	37.0	37.0	40.0	33.0	40.0
64-65	37.18548884931512	37.0	37.0	40.0	33.0	40.0
66-67	36.95479739799211	37.0	37.0	40.0	33.0	40.0
68-69	36.68969613851817	37.0	37.0	40.0	33.0	40.0
70-71	36.24504403347652	37.0	37.0	38.5	33.0	40.0
72-73	36.1507154845552	37.0	37.0	37.0	33.0	40.0
74-75	35.92323457477017	37.0	37.0	37.0	33.0	40.0
76-77	33.8612750530592	35.0	33.0	37.0	27.0	37.0
78-79	35.28936532796021	37.0	33.0	37.0	33.0	37.0
80-81	35.605691449111625	37.0	37.0	37.0	33.0	37.0
82-83	35.61174246633066	37.0	37.0	37.0	33.0	37.0
84-85	35.51226766574824	37.0	35.0	37.0	33.0	37.0
86-87	35.07743632545998	37.0	33.0	37.0	33.0	37.0
88-89	35.004770324048714	37.0	33.0	37.0	33.0	37.0
90-91	35.18134860766399	37.0	33.0	37.0	33.0	37.0
92-93	35.17919010926029	37.0	33.0	37.0	33.0	37.0
94-95	35.15328001046218	37.0	33.0	37.0	33.0	37.0
96-97	35.11299130392469	37.0	33.0	37.0	33.0	37.0
98-99	35.078029296806065	37.0	33.0	37.0	33.0	37.0
100	35.02323040832018	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	30.0
21	149.0
22	548.0
23	1633.0
24	4258.0
25	8822.0
26	16282.0
27	26238.0
28	40050.0
29	57548.0
30	79056.0
31	107000.0
32	143571.0
33	195445.0
34	280040.0
35	434738.0
36	868796.0
37	2442217.0
38	3306983.0
39	134774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97933968583422	20.010917772823436	14.507506091542874	23.502236449799476
2	14.723971555863628	20.6160343497482	41.68088047125113	22.97911362313704
3	18.2712615712999	26.002725308290835	30.298822948112004	25.427190172297266
4	12.510814939191475	17.96329947547008	38.631447615435555	30.894437969902892
5	13.521759724254782	37.521373826273	35.16138182408232	13.795484625389903
6	29.938580164262408	37.545430379439246	18.687125545305687	13.828863910992661
7	26.629559820828746	32.8025073568558	22.65731177825925	17.910621044056207
8	24.746194918081112	36.11921039564409	20.878068261116407	18.2565264251584
9	25.807106419566967	16.933998283298042	21.214744589652025	36.04415070748297
10-11	24.502250931557494	26.644305441385573	28.350587898012098	20.502855729044835
12-13	25.383202044333323	24.27202779712088	28.589500087258727	21.755270071287068
14-15	22.503479917360707	25.5571733670161	26.6160782859293	25.323268429693886
16-17	21.691973743345446	28.651108922211115	27.255368129970588	22.40154920447285
18-19	21.66539112651141	27.689520189902485	29.311802313694024	21.333286369892082
20-21	22.7044449694556	26.748241127396682	29.217007906872567	21.330305996275147
22-23	22.40214774880309	26.73087099852387	29.020948786665656	21.846032466007387
24-25	22.078091642372254	26.93434072505907	29.18316449246178	21.804403140106892
26-27	22.04864008739669	27.125765493528824	29.020840933929286	21.8047534851452
28-29	21.949984206852903	27.223370438979394	29.056433092672044	21.770212261495654
30-31	22.275453097919584	27.002882667597838	28.947236544719274	21.774427689763304
32-33	22.068139758679397	27.083752806770793	28.855947886594123	21.992159547955684
34-35	22.135871648792506	27.15296775079217	28.942383887465954	21.768776712949368
36-37	22.143292147372694	27.15321265592311	28.801891753460374	21.901603443243822
38-39	22.03513999836964	27.213825648555506	28.881924714399528	21.869109638675326
40-41	22.31136622184879	27.11516015489166	28.740175692834924	21.83329793042463
42-43	22.281260656497974	27.197272393055204	28.48026877867545	22.041198171771363
44-45	22.417574472727107	27.388068176547044	28.17531221778516	22.019045132940683
46-47	22.374285677345952	27.307824580456646	27.84654022186021	22.471349520337185
48-49	22.41912212519762	27.509709405590684	27.741941296180755	22.32922717303094
50-51	22.28729763486515	27.616079105902504	27.665600625047038	22.431022634185307
52-53	22.424467925294156	27.62850824090277	27.50868096144363	22.438342872359442
54-55	22.486228390925803	27.51070238262146	27.491946369826493	22.511122856626244
56-57	22.50205008335289	27.586088524426632	27.444448849486424	22.46741254273405
58-59	22.403956942681504	27.763486149228218	27.539572785515492	22.292984122574786
60-61	22.595475843074876	27.676467025289835	27.4240016591617	22.304055472473586
62-63	22.50033394324668	27.759148751941854	27.334480521195587	22.40603678361588
64-65	22.415417873366852	27.742375676039522	27.32764955757726	22.514556893016362
66-67	22.495143649071558	27.779505883392858	27.19685601699951	22.528494450536073
68-69	22.478128954848216	27.631456358298596	27.31268267168696	22.577732015166227
70-71	22.46108691675023	27.466158552941717	27.294103679116837	22.778650851191216
72-73	22.55434028634838	27.453706122657827	27.28627018854109	22.705683402452696
74-75	22.447124827294523	27.62916872091013	27.24251054697422	22.681195904821127
76-77	22.634373951738326	27.56002361007833	27.042922723615476	22.762679714567877
78-79	22.594864831009367	27.6025352994618	27.026329531843352	22.776270337685478
80-81	22.524620323646	27.637659404664372	27.10146837593851	22.736251895751113
82-83	22.59765459172323	27.567757088730318	27.251729735402336	22.58285858414412
84-85	22.786343513492916	27.55339233101169	27.107624038037514	22.55264011745788
86-87	22.621788654038888	27.6486037085496	27.095151179111543	22.63445645829997
88-89	22.6885821416328	27.637766866055657	27.09960420414675	22.574046788164797
90-91	22.657876983142764	27.70014330528744	27.161653332543416	22.48032637902638
92-93	22.735526087160554	27.66733559260983	27.133267613922452	22.46387070630716
94-95	22.49939215867432	27.76111872022049	27.196446369772538	22.543042751332656
96-97	22.486337527594774	27.857513038247735	27.327363272532164	22.32878616162533
98-99	23.70513121484981	29.698726867356264	27.40266704864625	19.19347486914768
100	22.67169315959393	30.709324851450436	21.27952342716638	25.33945856178925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2303.0
1	2073.5
2	2739.5
3	3868.0
4	5441.0
5	8997.0
6	11731.5
7	13311.5
8	14241.0
9	13893.0
10	12693.5
11	11504.0
12	12909.0
13	17666.0
14	27830.0
15	41279.0
16	52338.5
17	58895.5
18	59853.5
19	56153.0
20	49962.0
21	43237.0
22	38850.0
23	39632.5
24	44847.0
25	53679.5
26	66095.0
27	80455.5
28	95267.0
29	111900.5
30	127343.0
31	140904.0
32	158316.0
33	176996.5
34	195886.0
35	213624.5
36	230484.5
37	247153.5
38	258118.5
39	264825.0
40	275848.5
41	287443.5
42	295298.0
43	305317.5
44	315367.0
45	324833.0
46	333667.5
47	339314.5
48	339402.5
49	336874.0
50	332232.5
51	323068.5
52	312785.5
53	300352.0
54	287433.0
55	271405.5
56	251025.5
57	228776.5
58	205015.5
59	179923.5
60	150041.0
61	120651.0
62	95774.5
63	73615.0
64	54868.5
65	40788.0
66	30897.0
67	23462.5
68	17642.5
69	13012.0
70	9259.0
71	6313.0
72	4250.0
73	2769.0
74	1752.0
75	1146.0
76	747.5
77	486.5
78	325.0
79	228.5
80	164.0
81	113.0
82	79.5
83	47.5
84	25.0
85	15.0
86	11.0
87	10.0
88	6.5
89	4.0
90	3.5
91	3.5
92	3.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0013868124202429451
4	0.01088586386509285
5	0.011671315147354342
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.6818950664815246E-5
22-23	0.0
24-25	0.005806627399706932
26-27	0.046768813472070785
28-29	0.08251000979122879
30-31	0.0751191950490108
32-33	0.03450211961413126
34-35	0.0031673911145894676
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.3378166422353695E-4
56-57	4.644343573191018E-4
58-59	2.7141006417016E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.4287284866529255E-4
72-73	0.013071453653013721
74-75	0.029113194367127414
76-77	0.002433667175782021
78-79	0.0
80-81	0.0
82-83	0.007668625581163928
84-85	0.0470805390429035
86-87	0.03993753045773905
88-89	0.009922208336298368
90-91	1.0971027631865287E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	121.0
20-21	475.0
22-23	1189.0
24-25	2351.0
26-27	3970.0
28-29	8001.0
30-31	18465.0
32-33	23999.0
34-35	32272.0
36-37	46164.0
38-39	53238.0
40-41	57015.0
42-43	66324.0
44-45	79719.0
46-47	78355.0
48-49	67712.0
50-51	57049.0
52-53	52516.0
54-55	55853.0
56-57	59785.0
58-59	58297.0
60-61	59937.0
62-63	60004.0
64-65	61447.0
66-67	65474.0
68-69	63698.0
70-71	61344.0
72-73	58787.0
74-75	59281.0
76-77	58205.0
78-79	61531.0
80-81	62985.0
82-83	60438.0
84-85	61395.0
86-87	63798.0
88-89	68288.0
90-91	72512.0
92-93	76231.0
94-95	91613.0
96-97	262654.0
98-99	493116.0
100-101	5402574.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.73484425970697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.25883416023281	73.02080898790166
2	7.831251770703866	12.958347912154778
3	2.110328596467502	5.237931232966344
4	0.8437816453726642	2.7924057207642674
5	0.3737907741178084	1.5462760741176094
6	0.20248959073932718	1.0051766852418023
7	0.11746911609923809	0.6803152318057132
8	0.07680831894989826	0.5083779444935786
9	0.04599025392341302	0.34244968462361586
>10	0.13696085760019114	1.65746028448381
>50	0.0018212236014357476	0.10021896520361767
>100	3.724478312590342E-4	0.055556624761432104
>500	5.062220638714196E-5	0.027014021317085163
>1k	5.062215418260863E-5	0.0676606301647834
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2272676285335796E-5	0.0	0.0	0.0
3	0.0	1.2272676285335796E-5	0.0	6.136338142667898E-5	0.0
4	0.0	3.681802885600739E-5	0.0	8.590873399735058E-5	1.2272676285335796E-5
5	0.0	3.681802885600739E-5	0.0	9.818141028268637E-5	1.2272676285335796E-5
6	8.590873399735058E-5	3.681802885600739E-5	0.0	1.1045408656802217E-4	1.2272676285335796E-5
7	8.590873399735058E-5	3.681802885600739E-5	0.0	1.1045408656802217E-4	1.2272676285335796E-5
8	8.590873399735058E-5	6.136338142667898E-5	0.0	1.2272676285335796E-4	1.2272676285335796E-5
9	8.590873399735058E-5	1.5954479170936535E-4	0.0	1.7181746799470115E-4	2.454535257067159E-5
10-11	8.590873399735058E-5	1.5954479170936535E-4	0.0	1.9022648242270486E-4	2.454535257067159E-5
12-13	8.590873399735058E-5	1.5954479170936535E-4	0.0	2.3318084942138012E-4	3.068169071333949E-5
14-15	8.590873399735058E-5	2.2704451127871224E-4	0.0	2.577262019920517E-4	5.522704328401109E-5
16-17	1.8409014428003695E-4	2.3318084942138014E-4	0.0	2.6999887827738753E-4	7.363605771201478E-5
18-19	1.8409014428003695E-4	2.6386254013471963E-4	0.0	3.190895834187307E-4	8.590873399735058E-5
20-21	1.8409014428003695E-4	3.068169071333949E-4	0.0	3.497712741320702E-4	8.590873399735058E-5
22-23	1.8409014428003695E-4	3.436349359894023E-4	0.0	3.927256411307455E-4	8.590873399735058E-5
24-25	1.9022648242270486E-4	4.172709937014171E-4	0.0	4.295436699867529E-4	9.818141028268637E-5
26-27	1.9636282056537273E-4	4.540890225574245E-4	0.0	5.890884616961182E-4	9.818141028268637E-5
28-29	1.9636282056537273E-4	4.540890225574245E-4	0.0	0.0013193127006735981	9.818141028268637E-5
30-31	2.0249915870804063E-4	5.093160658414355E-4	0.0	0.0029699876610512627	1.0431774842535427E-4
32-33	2.0863549685070854E-4	5.277250802694393E-4	0.0	0.006866562381645378	1.3499943913869376E-4
34-35	2.2090817313604434E-4	5.890884616961182E-4	0.0	0.01182472360092104	1.4727211542402957E-4
36-37	2.2090817313604434E-4	6.504518431227972E-4	0.0	0.02005968938838136	1.4727211542402957E-4
38-39	2.2090817313604434E-4	6.688608575508008E-4	0.0	0.03967142609234796	1.5954479170936535E-4
40-41	2.3318084942138014E-4	6.995425482641404E-4	0.0	0.060074750416718725	1.5954479170936535E-4
42-43	2.454535257067159E-4	7.118152245494762E-4	0.0	0.07266037994733059	1.5954479170936535E-4
44-45	2.515898638493838E-4	7.731786059761551E-4	0.0	0.08922235659439123	1.5954479170936535E-4
46-47	2.577262019920517E-4	8.222693111174983E-4	0.0	0.1066802386102814	1.5954479170936535E-4
48-49	2.577262019920517E-4	8.222693111174983E-4	0.0	0.12165290367839107	1.5954479170936535E-4
50-51	2.7613521642005543E-4	9.08178045114849E-4	0.0	0.13650897832179004	1.5954479170936535E-4
52-53	2.8227155456272333E-4	9.08178045114849E-4	0.0	0.1817828811383938	1.9022648242270486E-4
54-55	2.8227155456272333E-4	9.449960739708564E-4	0.0	0.23818196500765448	2.2090817313604434E-4
56-57	2.9454423084805914E-4	0.001030904807968207	0.0	0.2959003615775887	2.2704451127871224E-4
58-59	2.9454423084805914E-4	0.0010370411461108747	0.0	0.338652229417556	2.3931718756404802E-4
60-61	3.252259215613986E-4	0.00107999551310955	0.0	0.3680268801065072	2.515898638493838E-4
62-63	3.9272564113074546E-4	0.0011168135419655574	0.0	0.3881479328763152	2.577262019920517E-4
64-65	3.9272564113074546E-4	0.0012886310099602587	0.0	0.393584728470719	2.577262019920517E-4
66-67	4.0499831741608127E-4	0.0013009036862455944	0.0	0.39884970659712804	2.577262019920517E-4
68-69	4.0499831741608127E-4	0.0013193127006735981	0.0	0.403764913449405	2.577262019920517E-4
70-71	4.356800081294208E-4	0.0013438580532442697	0.0	0.40827512198426597	2.6999887827738753E-4
72-73	4.909070514134318E-4	0.0013499943913869376	0.0	0.41174215303487327	3.313622597040665E-4
74-75	4.909070514134318E-4	0.0013622670676722735	0.0	0.4144175964650765	3.313622597040665E-4
76-77	4.909070514134318E-4	0.0013990850965282808	0.0	0.41720349398184775	3.374985978467344E-4
78-79	5.031797276987676E-4	0.0013990850965282808	0.0	0.41893394133808004	3.436349359894023E-4
80-81	5.031797276987676E-4	0.0014052214346709487	0.0	0.41960280219563084	3.62043950417406E-4
82-83	5.031797276987676E-4	0.0014113577728136165	0.0	0.4199587098079056	4.1727099370141707E-4
84-85	5.215887421267714E-4	0.0014236304490989524	0.0	0.42010598192332965	4.23407331844085E-4
86-87	5.277250802694393E-4	0.001448175801669624	0.0	0.42021643600989766	4.295436699867529E-4
88	5.277250802694393E-4	0.0015954479170936535	0.0	0.42021643600989766	4.295436699867529E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13230	0.0	50.102577	1
GTATCAA	24185	0.0	38.328613	1
ATCAACG	29360	0.0	31.093988	3
TCAACGC	29510	0.0	30.906876	4
TATCAAC	30055	0.0	30.559225	2
CAACGCA	29990	0.0	30.437435	5
AACGCAG	30525	0.0	29.968039	6
ACGCAGA	34965	0.0	26.126034	7
CGCAGAG	35520	0.0	25.705826	8
GCAGAGT	40080	0.0	22.78121	9
GAGTACT	24220	0.0	21.021088	12-13
TACATGG	27790	0.0	20.66387	2
GTACATG	28325	0.0	20.619486	1
GTGGTAT	6685	0.0	20.391956	1
TGGTATC	6590	0.0	19.9102	2
CAGAGTA	39360	0.0	19.849169	10-11
ACATGGG	28350	0.0	19.774847	3
AGAGTAC	37090	0.0	18.951273	10-11
GTACTTT	26960	0.0	18.853771	14-15
CATGGGG	21145	0.0	17.890106	4
>>END_MODULE