FastQCFastQC Report
Fri 27 May 2016
SRR2138657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8425133
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134000.051.46921
GTATCAA248150.037.6812741
ATCAACG295700.031.0889073
TCAACGC308750.029.7738044
TATCAAC311900.029.597082
AACGCAG315350.029.1854726
CAACGCA315750.029.1775555
ACGCAGA325050.028.3276377
GTGGTAT60800.026.2744141
CGCAGAG370400.024.836348
TGGTATC62100.024.0780262
GTACATG299700.022.401381
ACATGGG286750.022.180013
TACATGG293650.022.1230582
GCAGAGT421350.021.7017049
GAGTACT248450.020.66488612-13
CGAGACC72250.019.48003886-87
CAGAGTA407500.019.40314710-11
AGAGTAC382400.019.0952110-11
AGACCGA76750.018.84407288-89
Produced by FastQC (version 0.11.5)