##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138657_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8425133
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.844098484854776	33.0	33.0	33.0	33.0	33.0
2	32.88898513530884	33.0	33.0	33.0	33.0	33.0
3	33.55269074090581	33.0	33.0	33.0	33.0	37.0
4	36.71496806044486	37.0	37.0	37.0	37.0	37.0
5	36.73189752612807	37.0	37.0	37.0	37.0	37.0
6	36.73750705181746	37.0	37.0	37.0	37.0	37.0
7	36.729112169505214	37.0	37.0	37.0	37.0	37.0
8	36.71133856284524	37.0	37.0	37.0	37.0	37.0
9	36.71425483728269	37.0	37.0	37.0	37.0	37.0
10-11	36.69887887823255	37.0	37.0	37.0	37.0	37.0
12-13	36.929979740379174	37.0	37.0	37.0	37.0	38.5
14-15	38.895379040307134	40.0	40.0	40.0	37.0	40.0
16-17	38.84628242663943	40.0	37.0	40.0	37.0	40.0
18-19	38.880060587767574	40.0	40.0	40.0	37.0	40.0
20-21	38.91755356310583	40.0	40.0	40.0	37.0	40.0
22-23	38.9449695969422	40.0	40.0	40.0	37.0	40.0
24-25	38.9375310299023	40.0	40.0	40.0	37.0	40.0
26-27	38.84385238531155	40.0	40.0	40.0	37.0	40.0
28-29	38.771625766534044	40.0	37.0	40.0	37.0	40.0
30-31	38.67089736141554	40.0	37.0	40.0	37.0	40.0
32-33	38.4218250149619	40.0	37.0	40.0	37.0	40.0
34-35	38.52471588543063	40.0	37.0	40.0	37.0	40.0
36-37	38.51143227785683	40.0	37.0	40.0	37.0	40.0
38-39	38.52654466681808	40.0	37.0	40.0	37.0	40.0
40-41	38.45886574385152	40.0	37.0	40.0	37.0	40.0
42-43	38.20579986347883	40.0	37.0	40.0	37.0	40.0
44-45	38.07529370931003	40.0	37.0	40.0	35.0	40.0
46-47	38.095328509105926	40.0	37.0	40.0	35.0	40.0
48-49	37.98820145878028	40.0	37.0	40.0	33.0	40.0
50-51	38.06830613368719	40.0	37.0	40.0	37.0	40.0
52-53	38.04668462996554	40.0	37.0	40.0	37.0	40.0
54-55	37.97835694869088	40.0	37.0	40.0	33.0	40.0
56-57	37.85753126239028	40.0	37.0	40.0	33.0	40.0
58-59	37.72327012293894	40.0	37.0	40.0	33.0	40.0
60-61	37.46843225827169	38.5	37.0	40.0	33.0	40.0
62-63	37.28272483204795	37.0	37.0	40.0	33.0	40.0
64-65	37.10385250189957	37.0	37.0	40.0	33.0	40.0
66-67	36.86244931947659	37.0	37.0	40.0	33.0	40.0
68-69	36.56669595556518	37.0	37.0	40.0	33.0	40.0
70-71	36.165986890995725	37.0	37.0	38.5	33.0	40.0
72-73	36.04297256497733	37.0	37.0	37.0	33.0	40.0
74-75	35.580865093047564	37.0	35.0	37.0	33.0	40.0
76-77	33.98088254411479	35.0	33.0	37.0	27.0	37.0
78-79	35.27167198161716	37.0	33.0	37.0	33.0	37.0
80-81	35.55575851077661	37.0	35.0	37.0	33.0	37.0
82-83	35.54847106368746	37.0	37.0	37.0	33.0	37.0
84-85	35.41290704121316	37.0	33.0	37.0	33.0	37.0
86-87	35.03604669231699	37.0	33.0	37.0	33.0	37.0
88-89	34.99297933150238	37.0	33.0	37.0	33.0	37.0
90-91	35.136047103311306	37.0	33.0	37.0	33.0	37.0
92-93	35.12049095359863	37.0	33.0	37.0	33.0	37.0
94-95	35.09492883382653	37.0	33.0	37.0	33.0	37.0
96-97	35.054660150584006	37.0	33.0	37.0	33.0	37.0
98-99	35.01949940627388	37.0	33.0	37.0	33.0	37.0
100	34.94632524102944	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	2.0
18	2.0
19	6.0
20	40.0
21	169.0
22	656.0
23	1905.0
24	4718.0
25	9568.0
26	17260.0
27	28606.0
28	42871.0
29	61672.0
30	84431.0
31	113955.0
32	153160.0
33	207718.0
34	297836.0
35	461462.0
36	913412.0
37	2521071.0
38	3348419.0
39	156191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.13284030003594	0.0	24.324520327534646	29.542639372429413
2	15.27376775932902	23.40920482763426	38.142051843074704	23.174975569962015
3	16.872147655268623	24.465098380781562	32.68921634015113	25.97353762379869
4	13.349128308712594	22.661433171442553	36.90352482218485	27.085913697660004
5	17.629350458192867	36.276031527467836	31.754558188120225	14.340059826219079
6	28.422459324974454	36.631801539512786	20.104382921907582	14.841356213605176
7	26.579319281962672	32.91362878188392	21.912888496834412	18.594163439319
8	24.694779299033023	31.719962165582434	21.82786906746754	21.757389467917005
9	24.338962957617404	20.37760116071758	23.27451685332445	32.00891902834056
10-11	25.09442877637659	25.80330779347934	28.012068177440046	21.09019525270402
12-13	25.39287510357403	23.901325949394508	28.138054319142498	22.56774462788896
14-15	22.77922496891147	26.174607569993256	26.490857770435195	24.555309690660078
16-17	21.72083811614606	28.585370699785983	27.31018014789796	22.38361103616999
18-19	21.82166738495404	27.540716567916494	29.255823023802712	21.381793023326754
20-21	22.756497300140868	26.723667649311256	29.196419796403134	21.323415254144745
22-23	22.641154883166685	26.447783900577853	28.94294677112892	21.968114445126545
24-25	22.19893606283879	26.759165474289176	29.233638700714238	21.808259762157796
26-27	22.155541682454135	27.022261178345214	28.94489716215326	21.87729997704739
28-29	22.12806037004807	26.91630592291358	28.9988320888162	21.956801618222148
30-31	22.32002295719958	26.793151594394544	29.02413024864126	21.862695199764616
32-33	22.23598768376752	27.015408180181055	28.757470734984146	21.99113340106728
34-35	22.289425756342023	26.852563014436235	28.84667043831123	22.011340790910513
36-37	22.260134478169217	26.995245965456494	28.852573697998125	21.892045858376168
38-39	22.112791415125155	27.22039875630618	28.797621496062035	21.869188332506624
40-41	22.43696182318084	26.946199124692367	28.486646540956485	22.130192511170304
42-43	22.30849248637969	27.07441629706257	28.49109024294252	22.126000973615223
44-45	22.486221255691643	27.35529370960937	28.083931867186106	22.074553167512878
46-47	22.547413034214113	27.067938300158257	27.814508836127988	22.570139829499645
48-49	22.424920121341827	27.32289460966139	27.788417884941403	22.463767384055377
50-51	22.360879954185258	27.645583988060775	27.568519538260013	22.42501651949396
52-53	22.533378888054063	27.36779989484117	27.468272032463958	22.63054918464081
54-55	22.555122663014693	27.313458789590783	27.55693743676461	22.57448111062991
56-57	22.54512707478766	27.588969005219553	27.404156724769113	22.461747195223676
58-59	22.55639210918844	27.395427741818274	27.42450467770216	22.623675471291126
60-61	22.72761928107967	27.37750154114605	27.48325167153804	22.411627506236247
62-63	22.450669165442356	27.532068156520705	27.427216570045054	22.590046107991885
64-65	22.54873753558749	27.327651389578143	27.368625142400198	22.75498593243416
66-67	22.496319152157497	27.48789541034605	27.42378754969812	22.59199788779834
68-69	22.537738217780824	27.590889038462603	27.363931042564904	22.507441701191674
70-71	22.585817954876163	27.198791003409934	27.21701428253801	22.998376759175894
72-73	22.518908671096046	27.33749377658335	27.220316896757534	22.923280655563065
74-75	22.475987933043516	27.485140389010894	27.36746795590015	22.671403722045444
76-77	22.76368680402108	27.156775775248814	27.0334841204516	23.0460533002785
78-79	22.629265290398088	27.243703031333293	27.016275521713702	23.110756156554917
80-81	22.77956634870561	27.48698268963969	26.988007380073803	22.7454435815809
82-83	22.833394209867752	27.088318833286383	27.116728690009882	22.961558266835983
84-85	22.76874363359317	27.22038917319371	27.07734726603872	22.933519927174395
86-87	22.705660778381297	27.420478652489095	26.993126768312376	22.880733800817236
88-89	22.95718407921827	27.243583527493925	27.067343242855685	22.73188915043212
90-91	22.88795233114484	27.316243969681825	27.102401583609215	22.693402115564115
92-93	22.802562977864334	27.200415677710847	27.09727829084513	22.899743053579687
94-95	22.937825011767735	27.214277231034984	27.12476005946981	22.723137697727473
96-97	22.985080583896643	27.387457596367266	27.3637249985828	22.26373682115329
98-99	23.874574235177416	29.54440978536349	26.882729528895315	19.698286450563774
100	23.049058890871745	29.7153663984197	21.255665173911577	25.979909536796974
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1798.0
1	1651.0
2	2413.0
3	3584.0
4	5101.5
5	8438.0
6	11116.0
7	13062.5
8	14754.0
9	15095.5
10	14128.0
11	12878.0
12	13856.5
13	17738.0
14	27304.5
15	41439.5
16	53944.0
17	61483.5
18	62199.0
19	57392.5
20	50412.5
21	43514.0
22	39079.0
23	39433.5
24	44995.0
25	55096.0
26	68670.5
27	83956.5
28	98705.0
29	115784.0
30	131396.0
31	145520.0
32	164147.5
33	181600.0
34	198641.0
35	216019.5
36	233961.5
37	250901.5
38	261108.0
39	266796.5
40	276235.0
41	286531.0
42	292684.5
43	300900.5
44	311510.0
45	321946.5
46	330277.5
47	335243.5
48	337964.0
49	341596.0
50	342952.5
51	337836.0
52	328485.5
53	317415.5
54	306348.0
55	290447.0
56	269770.0
57	247342.0
58	223729.5
59	198756.5
60	168735.5
61	137692.0
62	109584.5
63	85196.0
64	64920.5
65	48741.5
66	37112.0
67	28437.5
68	21406.0
69	15169.5
70	10450.0
71	7514.5
72	5310.0
73	3628.5
74	2450.0
75	1648.0
76	1094.0
77	710.5
78	474.0
79	317.5
80	215.5
81	150.5
82	109.5
83	79.5
84	58.0
85	43.0
86	30.5
87	18.0
88	14.0
89	13.0
90	9.5
91	6.0
92	3.5
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0682323946696154E-4
2	6.409394368017692E-4
3	0.003157220188690197
4	0.011097747655734336
5	0.008700159392142533
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.30871283675955E-5
22-23	4.5701679851304543E-4
24-25	0.01010400715900371
26-27	0.05016397270633297
28-29	0.0822433118557528
30-31	0.06987912690306516
32-33	0.029333090093988184
34-35	0.00233427905551957
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.453699814493837E-4
56-57	4.163447042710071E-4
58-59	2.6177743211522185E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	6.7560181596363315E-6
68-69	1.1589563121192061E-4
70-71	0.001355204732567544
72-73	0.014879152343574065
74-75	0.02523116196921798
76-77	0.0022107954625718686
78-79	5.702077972637581E-5
80-81	7.123850442603389E-4
82-83	0.011892942305208208
84-85	0.04490880607925733
86-87	0.03510750831738284
88-89	0.007951746228180524
90-91	1.2119134424151375E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	151.0
20-21	521.0
22-23	1162.0
24-25	3041.0
26-27	3683.0
28-29	9705.0
30-31	19739.0
32-33	25388.0
34-35	35312.0
36-37	47595.0
38-39	53599.0
40-41	58288.0
42-43	67938.0
44-45	81641.0
46-47	78908.0
48-49	68443.0
50-51	57818.0
52-53	54795.0
54-55	56507.0
56-57	55357.0
58-59	42198.0
60-61	61864.0
62-63	60116.0
64-65	63528.0
66-67	67132.0
68-69	66141.0
70-71	64362.0
72-73	62490.0
74-75	63544.0
76-77	62854.0
78-79	65813.0
80-81	66770.0
82-83	65268.0
84-85	66985.0
86-87	70426.0
88-89	75105.0
90-91	79145.0
92-93	85272.0
94-95	110891.0
96-97	419634.0
98-99	1471154.0
100-101	4454850.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.03097318895942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.35781902861372	72.4807788377114
2	7.728128297093568	12.678917702794424
3	2.0881440189294787	5.138774580944702
4	0.7837488639945015	2.571667281968415
5	0.3717453749462052	1.5247317442665915
6	0.19871528327155666	0.9780484844571325
7	0.12847287088246268	0.7377128238807559
8	0.08438797218113807	0.5537941986769273
9	0.05318809936983152	0.3926764398040524
>10	0.20120190649672537	2.5439563541337846
>50	0.00366686375384072	0.19238508303825344
>100	6.738060822721587E-4	0.11017995545420702
>500	5.380724118823633E-5	0.02584315227439419
>1k	5.380714358294693E-5	0.07053336059498029
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7803873244493588E-4	0.0	0.0	0.0
3	0.0	1.7803873244493588E-4	0.0	0.0	0.0
4	0.0	1.899079812745983E-4	0.0	0.0	0.0
5	0.0	1.899079812745983E-4	0.0	0.0	0.0
6	0.0	2.0177723010426067E-4	0.0	0.0	0.0
7	0.0	2.0177723010426067E-4	0.0	0.0	0.0
8	0.0	2.2551572776358545E-4	0.0	0.0	0.0
9	0.0	4.1542370903818374E-4	0.0	0.0	0.0
10-11	0.0	4.1542370903818374E-4	0.0	1.1869248829662393E-5	0.0
12-13	0.0	4.1542370903818374E-4	0.0	4.154237090381838E-5	0.0
14-15	0.0	5.993970658979507E-4	0.0	5.9346244148311964E-5	0.0
16-17	0.0	7.002856809500812E-4	0.0	7.715011739280555E-5	0.0
18-19	0.0	7.002856809500812E-4	0.0	3.4420821606020936E-4	0.0
20-21	0.0	7.062203053649124E-4	0.0	9.198667842988354E-4	0.0
22-23	0.0	8.367820424911986E-4	0.0	0.0016557602117379037	0.0
24-25	0.0	8.78324413395017E-4	0.0	0.0028842274656079615	0.0
26-27	0.0	9.139321598840042E-4	0.0	0.007531038382420788	0.0
28-29	0.0	9.139321598840042E-4	0.0	0.01894332113214118	0.0
30-31	0.0	0.0011038401411586026	0.0	0.020937354935524462	0.0
32-33	0.0	0.0011275786388179273	0.0	0.024367567847296892	0.0
34-35	0.0	0.0012284672538700576	0.0	0.028699843670123664	0.0
36-37	0.0	0.001335290493337019	0.0	0.03548905400069055	0.0
38-39	0.0	0.0013887021130705	0.0	0.05446204825490589	0.0
40-41	0.0	0.0015073946013671239	0.0	0.07136385858834512	0.0
42-43	0.0	0.0017447795779603715	0.0	0.081915620797915	0.0
44-45	0.0	0.0019821645545536194	0.0	0.09715573629520151	0.0
46-47	0.0	0.0019821645545536194	0.0	0.1128468832480152	0.0
48-49	0.0	0.0019880991789684504	0.0	0.1274579285573296	0.0
50-51	0.0	0.002201745657902374	0.0	0.14477516259980705	0.0
52-53	0.0	0.0022551572776358547	0.0	0.1857656134330461	0.0
54-55	0.0	0.0022610919020506857	0.0	0.23664314854139396	0.0
56-57	0.0	0.002302634272954504	0.0	0.2946422329475392	0.0
58-59	0.0	0.0023085688973693355	0.0	0.502003944626156	0.0
60-61	0.0	0.0023441766438583225	0.0	0.6290345802256179	0.0
62-63	0.0	0.0024094575124214658	0.0	0.6357881828096956	0.0
64-65	0.0	0.0027655349773113374	0.0	0.6412421026469255	0.0
66-67	0.0	0.0027655349773113374	0.0	0.646571395371444	0.0
68-69	0.0	0.002842685094704143	0.0	0.6517582571100065	1.1869248829662393E-5
70-71	0.0	0.0028842274656079615	0.0	0.656482218144212	1.1869248829662393E-5
72-73	0.0	0.0028901620900227925	0.0	0.6599124310559845	1.1869248829662393E-5
74-75	0.0	0.002913900587682117	0.0	0.6625474042961697	1.1869248829662393E-5
76-77	1.1869248829662393E-5	0.003038527700393572	0.0	0.664885646315613	1.1869248829662393E-5
78-79	1.1869248829662393E-5	0.003086004695712222	0.0	0.6662802830530984	1.1869248829662393E-5
80-81	1.1869248829662393E-5	0.0030919393201270535	0.0	0.6667965953771887	1.1869248829662393E-5
82-83	1.1869248829662393E-5	0.0030978739445418845	0.0	0.6672357575838862	1.1869248829662393E-5
84-85	1.1869248829662393E-5	0.0031038085689567155	0.0	0.667490946433724	1.1869248829662393E-5
86-87	3.5607746488987174E-5	0.0031631548131050274	0.0	0.6675799657999464	1.1869248829662393E-5
88	3.5607746488987174E-5	0.00332338967230547	0.0	0.6675859004243613	1.1869248829662393E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13400	0.0	51.4692	1
GTATCAA	24815	0.0	37.681274	1
ATCAACG	29570	0.0	31.088907	3
TCAACGC	30875	0.0	29.773804	4
TATCAAC	31190	0.0	29.59708	2
AACGCAG	31535	0.0	29.185472	6
CAACGCA	31575	0.0	29.177555	5
ACGCAGA	32505	0.0	28.327637	7
GTGGTAT	6080	0.0	26.274414	1
CGCAGAG	37040	0.0	24.83634	8
TGGTATC	6210	0.0	24.078026	2
GTACATG	29970	0.0	22.40138	1
ACATGGG	28675	0.0	22.18001	3
TACATGG	29365	0.0	22.123058	2
GCAGAGT	42135	0.0	21.701704	9
GAGTACT	24845	0.0	20.664886	12-13
CGAGACC	7225	0.0	19.480038	86-87
CAGAGTA	40750	0.0	19.403147	10-11
AGAGTAC	38240	0.0	19.09521	10-11
AGACCGA	7675	0.0	18.844072	88-89
>>END_MODULE